Silencing of the tomato phosphatidylinositol‐phospholipase C2 (SlPLC2) reduces plant susceptibility to Botrytis cinerea

Autores
Gonorazky, Ana Gabriela; Guzzo, María Carla; Abd El Haliem, Ahmed M.; Joosten, Matthieu H. A. J.; Laxalt, Ana Maria
Año de publicación
2016
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The tomato [Solanum lycopersicum (Sl)] phosphatidylinositol‐phospholipase C (PI‐PLC) gene family is composed of six members, named SlPLC1 to SlPLC6, differentially regulated on pathogen attack. We have previously shown that the fungal elicitor xylanase induces a raise of SlPLC2 and SlPLC5 transcripts and that SlPLC2, but not SlPLC5, is required for xylanase‐induced expression of defense‐related genes. In this work we studied the role of SlPLC2 in the interaction between tomato and the necrotrophic fungus Botrytis cinerea. Inoculation of tomato leaves with B. cinerea increases SlPLC2 transcript levels. We knocked‐down the expression of SlPLC2 by virus‐induced gene silencing and plant defense responses were analyzed upon B. cinerea inoculation. SlPLC2 silenced plants developed smaller necrotic lesions concomitantly with less proliferation of the fungus. Silencing of SlPLC2 resulted as well in a reduced production of reactive oxygen species. Upon B. cinerea inoculation, transcript levels of the salicylic acid (SA)‐defense pathway marker gene SlPR1a were diminished in SlPLC2 silenced plants compared to non‐silenced infected plants, while transcripts of the jasmonic acid (JA)‐defense gene markers Proteinase Inhibitor I and II (SlPI‐I and SlPI‐II) were increased. This implies that SlPLC2 participates in plant susceptibility to B. cinerea.
Instituto de Fisiología y Recursos Genéticos Vegetales
Fil: Gonorazky, Ana Gabriela. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones Biológicas. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Instituto de Investigaciones Biológicas; Argentina
Fil: Guzzo, Maria Carla. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Fisiología y Recursos Genéticos Vegetales; Argentina
Fil: Abd El Haliem, Ahmed M. Wageningen University. Laboratory of Phytopathology; Holanda
Fil: Joosten, Matthieu H. A. J. Wageningen University. Laboratory of Phytopathology; Holanda
Fil: Laxalt, Ana Maria. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones Biológicas. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Instituto de Investigaciones Biológicas; Argentina
Fuente
Molecular plant pathology 17 (9) : 1354–1363. (December 2016)
Materia
Tomate
Tomatoes
Solanum Lycopersicum
Botrytis Cinerea
Fosfatidilinositoles
Phosphatidylinositols
Cell Death
Muerte Celular
Jasmonic Acid
Phospholipids
Fosfolípidos
Reactive Oxygen Species
Especies de Oxígeno Reactivo
Salicylic Acids
Ácido Salicílico
Ácido Jasmónico
Necrotrophic Fungus
Phospholipid Signalling
Nivel de accesibilidad
acceso restringido
Condiciones de uso
Repositorio
INTA Digital (INTA)
Institución
Instituto Nacional de Tecnología Agropecuaria
OAI Identificador
oai:localhost:20.500.12123/4719
Acceder
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oai_identifier_str oai:localhost:20.500.12123/4719
network_acronym_str INTADig
repository_id_str l
network_name_str INTA Digital (INTA)
spelling Silencing of the tomato phosphatidylinositol‐phospholipase C2 (SlPLC2) reduces plant susceptibility to Botrytis cinereaGonorazky, Ana GabrielaGuzzo, María CarlaAbd El Haliem, Ahmed M.Joosten, Matthieu H. A. J.Laxalt, Ana MariaTomateTomatoesSolanum LycopersicumBotrytis CinereaFosfatidilinositolesPhosphatidylinositolsCell DeathMuerte CelularJasmonic AcidPhospholipidsFosfolípidosReactive Oxygen SpeciesEspecies de Oxígeno ReactivoSalicylic AcidsÁcido SalicílicoÁcido JasmónicoNecrotrophic FungusPhospholipid SignallingThe tomato [Solanum lycopersicum (Sl)] phosphatidylinositol‐phospholipase C (PI‐PLC) gene family is composed of six members, named SlPLC1 to SlPLC6, differentially regulated on pathogen attack. We have previously shown that the fungal elicitor xylanase induces a raise of SlPLC2 and SlPLC5 transcripts and that SlPLC2, but not SlPLC5, is required for xylanase‐induced expression of defense‐related genes. In this work we studied the role of SlPLC2 in the interaction between tomato and the necrotrophic fungus Botrytis cinerea. Inoculation of tomato leaves with B. cinerea increases SlPLC2 transcript levels. We knocked‐down the expression of SlPLC2 by virus‐induced gene silencing and plant defense responses were analyzed upon B. cinerea inoculation. SlPLC2 silenced plants developed smaller necrotic lesions concomitantly with less proliferation of the fungus. Silencing of SlPLC2 resulted as well in a reduced production of reactive oxygen species. Upon B. cinerea inoculation, transcript levels of the salicylic acid (SA)‐defense pathway marker gene SlPR1a were diminished in SlPLC2 silenced plants compared to non‐silenced infected plants, while transcripts of the jasmonic acid (JA)‐defense gene markers Proteinase Inhibitor I and II (SlPI‐I and SlPI‐II) were increased. This implies that SlPLC2 participates in plant susceptibility to B. cinerea.Instituto de Fisiología y Recursos Genéticos VegetalesFil: Gonorazky, Ana Gabriela. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones Biológicas. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Instituto de Investigaciones Biológicas; ArgentinaFil: Guzzo, Maria Carla. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Fisiología y Recursos Genéticos Vegetales; ArgentinaFil: Abd El Haliem, Ahmed M. Wageningen University. Laboratory of Phytopathology; HolandaFil: Joosten, Matthieu H. A. J. Wageningen University. Laboratory of Phytopathology; HolandaFil: Laxalt, Ana Maria. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones Biológicas. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Instituto de Investigaciones Biológicas; ArgentinaWiley2019-03-25T13:39:46Z2019-03-25T13:39:46Z2016-12info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://hdl.handle.net/20.500.12123/4719https://onlinelibrary.wiley.com/doi/full/10.1111/mpp.123651364-3703 (Online)https://doi.org/10.1111/mpp.12365Molecular plant pathology 17 (9) : 1354–1363. (December 2016)reponame:INTA Digital (INTA)instname:Instituto Nacional de Tecnología Agropecuariaenginfo:eu-repo/semantics/restrictedAccess2025-09-29T13:44:36Zoai:localhost:20.500.12123/4719instacron:INTAInstitucionalhttp://repositorio.inta.gob.ar/Organismo científico-tecnológicoNo correspondehttp://repositorio.inta.gob.ar/oai/requesttripaldi.nicolas@inta.gob.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:l2025-09-29 13:44:36.834INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuariafalse
dc.title.none.fl_str_mv Silencing of the tomato phosphatidylinositol‐phospholipase C2 (SlPLC2) reduces plant susceptibility to Botrytis cinerea
title Silencing of the tomato phosphatidylinositol‐phospholipase C2 (SlPLC2) reduces plant susceptibility to Botrytis cinerea
spellingShingle Silencing of the tomato phosphatidylinositol‐phospholipase C2 (SlPLC2) reduces plant susceptibility to Botrytis cinerea
Gonorazky, Ana Gabriela
Tomate
Tomatoes
Solanum Lycopersicum
Botrytis Cinerea
Fosfatidilinositoles
Phosphatidylinositols
Cell Death
Muerte Celular
Jasmonic Acid
Phospholipids
Fosfolípidos
Reactive Oxygen Species
Especies de Oxígeno Reactivo
Salicylic Acids
Ácido Salicílico
Ácido Jasmónico
Necrotrophic Fungus
Phospholipid Signalling
title_short Silencing of the tomato phosphatidylinositol‐phospholipase C2 (SlPLC2) reduces plant susceptibility to Botrytis cinerea
title_full Silencing of the tomato phosphatidylinositol‐phospholipase C2 (SlPLC2) reduces plant susceptibility to Botrytis cinerea
title_fullStr Silencing of the tomato phosphatidylinositol‐phospholipase C2 (SlPLC2) reduces plant susceptibility to Botrytis cinerea
title_full_unstemmed Silencing of the tomato phosphatidylinositol‐phospholipase C2 (SlPLC2) reduces plant susceptibility to Botrytis cinerea
title_sort Silencing of the tomato phosphatidylinositol‐phospholipase C2 (SlPLC2) reduces plant susceptibility to Botrytis cinerea
dc.creator.none.fl_str_mv Gonorazky, Ana Gabriela
Guzzo, María Carla
Abd El Haliem, Ahmed M.
Joosten, Matthieu H. A. J.
Laxalt, Ana Maria
author Gonorazky, Ana Gabriela
author_facet Gonorazky, Ana Gabriela
Guzzo, María Carla
Abd El Haliem, Ahmed M.
Joosten, Matthieu H. A. J.
Laxalt, Ana Maria
author_role author
author2 Guzzo, María Carla
Abd El Haliem, Ahmed M.
Joosten, Matthieu H. A. J.
Laxalt, Ana Maria
author2_role author
author
author
author
dc.subject.none.fl_str_mv Tomate
Tomatoes
Solanum Lycopersicum
Botrytis Cinerea
Fosfatidilinositoles
Phosphatidylinositols
Cell Death
Muerte Celular
Jasmonic Acid
Phospholipids
Fosfolípidos
Reactive Oxygen Species
Especies de Oxígeno Reactivo
Salicylic Acids
Ácido Salicílico
Ácido Jasmónico
Necrotrophic Fungus
Phospholipid Signalling
topic Tomate
Tomatoes
Solanum Lycopersicum
Botrytis Cinerea
Fosfatidilinositoles
Phosphatidylinositols
Cell Death
Muerte Celular
Jasmonic Acid
Phospholipids
Fosfolípidos
Reactive Oxygen Species
Especies de Oxígeno Reactivo
Salicylic Acids
Ácido Salicílico
Ácido Jasmónico
Necrotrophic Fungus
Phospholipid Signalling
dc.description.none.fl_txt_mv The tomato [Solanum lycopersicum (Sl)] phosphatidylinositol‐phospholipase C (PI‐PLC) gene family is composed of six members, named SlPLC1 to SlPLC6, differentially regulated on pathogen attack. We have previously shown that the fungal elicitor xylanase induces a raise of SlPLC2 and SlPLC5 transcripts and that SlPLC2, but not SlPLC5, is required for xylanase‐induced expression of defense‐related genes. In this work we studied the role of SlPLC2 in the interaction between tomato and the necrotrophic fungus Botrytis cinerea. Inoculation of tomato leaves with B. cinerea increases SlPLC2 transcript levels. We knocked‐down the expression of SlPLC2 by virus‐induced gene silencing and plant defense responses were analyzed upon B. cinerea inoculation. SlPLC2 silenced plants developed smaller necrotic lesions concomitantly with less proliferation of the fungus. Silencing of SlPLC2 resulted as well in a reduced production of reactive oxygen species. Upon B. cinerea inoculation, transcript levels of the salicylic acid (SA)‐defense pathway marker gene SlPR1a were diminished in SlPLC2 silenced plants compared to non‐silenced infected plants, while transcripts of the jasmonic acid (JA)‐defense gene markers Proteinase Inhibitor I and II (SlPI‐I and SlPI‐II) were increased. This implies that SlPLC2 participates in plant susceptibility to B. cinerea.
Instituto de Fisiología y Recursos Genéticos Vegetales
Fil: Gonorazky, Ana Gabriela. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones Biológicas. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Instituto de Investigaciones Biológicas; Argentina
Fil: Guzzo, Maria Carla. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Fisiología y Recursos Genéticos Vegetales; Argentina
Fil: Abd El Haliem, Ahmed M. Wageningen University. Laboratory of Phytopathology; Holanda
Fil: Joosten, Matthieu H. A. J. Wageningen University. Laboratory of Phytopathology; Holanda
Fil: Laxalt, Ana Maria. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones Biológicas. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Instituto de Investigaciones Biológicas; Argentina
description The tomato [Solanum lycopersicum (Sl)] phosphatidylinositol‐phospholipase C (PI‐PLC) gene family is composed of six members, named SlPLC1 to SlPLC6, differentially regulated on pathogen attack. We have previously shown that the fungal elicitor xylanase induces a raise of SlPLC2 and SlPLC5 transcripts and that SlPLC2, but not SlPLC5, is required for xylanase‐induced expression of defense‐related genes. In this work we studied the role of SlPLC2 in the interaction between tomato and the necrotrophic fungus Botrytis cinerea. Inoculation of tomato leaves with B. cinerea increases SlPLC2 transcript levels. We knocked‐down the expression of SlPLC2 by virus‐induced gene silencing and plant defense responses were analyzed upon B. cinerea inoculation. SlPLC2 silenced plants developed smaller necrotic lesions concomitantly with less proliferation of the fungus. Silencing of SlPLC2 resulted as well in a reduced production of reactive oxygen species. Upon B. cinerea inoculation, transcript levels of the salicylic acid (SA)‐defense pathway marker gene SlPR1a were diminished in SlPLC2 silenced plants compared to non‐silenced infected plants, while transcripts of the jasmonic acid (JA)‐defense gene markers Proteinase Inhibitor I and II (SlPI‐I and SlPI‐II) were increased. This implies that SlPLC2 participates in plant susceptibility to B. cinerea.
publishDate 2016
dc.date.none.fl_str_mv 2016-12
2019-03-25T13:39:46Z
2019-03-25T13:39:46Z
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/20.500.12123/4719
https://onlinelibrary.wiley.com/doi/full/10.1111/mpp.12365
1364-3703 (Online)
https://doi.org/10.1111/mpp.12365
url http://hdl.handle.net/20.500.12123/4719
https://onlinelibrary.wiley.com/doi/full/10.1111/mpp.12365
https://doi.org/10.1111/mpp.12365
identifier_str_mv 1364-3703 (Online)
dc.language.none.fl_str_mv eng
language eng
dc.rights.none.fl_str_mv info:eu-repo/semantics/restrictedAccess
eu_rights_str_mv restrictedAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Wiley
publisher.none.fl_str_mv Wiley
dc.source.none.fl_str_mv Molecular plant pathology 17 (9) : 1354–1363. (December 2016)
reponame:INTA Digital (INTA)
instname:Instituto Nacional de Tecnología Agropecuaria
reponame_str INTA Digital (INTA)
collection INTA Digital (INTA)
instname_str Instituto Nacional de Tecnología Agropecuaria
repository.name.fl_str_mv INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuaria
repository.mail.fl_str_mv tripaldi.nicolas@inta.gob.ar
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score 12.559606

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