Enhanced sensitivity in detection of antiviral antibody responses using biotinylation of foot-and-mouth disease virus (FMDV) capsids

Autores
Kenney, Mary; Waters, Ryan A.; Rieder, Elizabeth; Pega, Juan Franco; Perez Filgueira, Daniel Mariano; Golde, William T.
Año de publicación
2017
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Analysis of the immune response to infection of livestock by foot-and-mouth disease virus (FMDV) is most often reported as the serum antibody response to the virus. While measurement of neutralizing antibody has been sensitive and specific, measurements of the quality of the antibody response are less robust. Determining the immunoglobulin (Ig) isotype of the serum antibody response provides a deeper understanding of the biology of the response and more sensitive methods for these assays will facilitate analyses of B cell mediated immunity. We tested the hypothesis that using the virus as the molecular probe could be achieved by adding tags to the surface of the FMDV capsid, and that would enhance sensitivity in assays for anti-FMDV antibody responses. The use of a FLAG-tagged virus in these assays failed to yield improvement whereas chemically biotinylating the virus capsid resulted in significant enhancement of the signal. Here we describe methods using biotinylated virus for measuring anti-viral antibody in serum and antibody secreting cells (ASCs) in blood that are sensitive and specific. Finally, we describe using the biotinylated virus in flow cytometry where such assays should greatly enhance the analysis of anti-virus antibody producing B cells, allowing the investigator to focus on only the FMDV specific B cells when analyzing the development of the B cell response to either infection or vaccination.
Instituto de Virología
Fil: Kenney, Mary. United States Department of Agriculture. Agricultural Research Service. Plum Island Animal Disease Center; Estados Unidos
Fil: Waters, Ryan A. United States Department of Agriculture. Agricultural Research Service. Plum Island Animal Disease Center; Estados Unidos
Fil: Rieder, Elizabeth. United States Department of Agriculture. Agricultural Research Service. Plum Island Animal Disease Center; Estados Unidos
Fil: Pega, Juan Franco. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Perez Filgueira, Daniel Mariano. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Golde, William T. United States Department of Agriculture. Agricultural Research Service. Plum Island Animal Disease Center; Estados Unidos
Fuente
Journal of immunological methods 450 : 1-9. (November 2017)
Materia
Virus Fiebre Aftosa
Respuesta Inmunológica
ELISA
Citometría de Flujo (Células)
Aphthovirus
Immune Response
Flow Cytometry
Foot and Mouth Disease Virus
Biotinylation
Biotinilación
Nivel de accesibilidad
acceso restringido
Condiciones de uso
Repositorio
INTA Digital (INTA)
Institución
Instituto Nacional de Tecnología Agropecuaria
OAI Identificador
oai:localhost:20.500.12123/4444
Acceder
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oai_identifier_str oai:localhost:20.500.12123/4444
network_acronym_str INTADig
repository_id_str l
network_name_str INTA Digital (INTA)
spelling Enhanced sensitivity in detection of antiviral antibody responses using biotinylation of foot-and-mouth disease virus (FMDV) capsidsKenney, MaryWaters, Ryan A.Rieder, ElizabethPega, Juan FrancoPerez Filgueira, Daniel MarianoGolde, William T.Virus Fiebre AftosaRespuesta InmunológicaELISACitometría de Flujo (Células)AphthovirusImmune ResponseFlow CytometryFoot and Mouth Disease VirusBiotinylationBiotinilaciónAnalysis of the immune response to infection of livestock by foot-and-mouth disease virus (FMDV) is most often reported as the serum antibody response to the virus. While measurement of neutralizing antibody has been sensitive and specific, measurements of the quality of the antibody response are less robust. Determining the immunoglobulin (Ig) isotype of the serum antibody response provides a deeper understanding of the biology of the response and more sensitive methods for these assays will facilitate analyses of B cell mediated immunity. We tested the hypothesis that using the virus as the molecular probe could be achieved by adding tags to the surface of the FMDV capsid, and that would enhance sensitivity in assays for anti-FMDV antibody responses. The use of a FLAG-tagged virus in these assays failed to yield improvement whereas chemically biotinylating the virus capsid resulted in significant enhancement of the signal. Here we describe methods using biotinylated virus for measuring anti-viral antibody in serum and antibody secreting cells (ASCs) in blood that are sensitive and specific. Finally, we describe using the biotinylated virus in flow cytometry where such assays should greatly enhance the analysis of anti-virus antibody producing B cells, allowing the investigator to focus on only the FMDV specific B cells when analyzing the development of the B cell response to either infection or vaccination.Instituto de VirologíaFil: Kenney, Mary. United States Department of Agriculture. Agricultural Research Service. Plum Island Animal Disease Center; Estados UnidosFil: Waters, Ryan A. United States Department of Agriculture. Agricultural Research Service. Plum Island Animal Disease Center; Estados UnidosFil: Rieder, Elizabeth. United States Department of Agriculture. Agricultural Research Service. Plum Island Animal Disease Center; Estados UnidosFil: Pega, Juan Franco. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Perez Filgueira, Daniel Mariano. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Golde, William T. United States Department of Agriculture. Agricultural Research Service. Plum Island Animal Disease Center; Estados UnidosElsevier2019-02-14T15:50:37Z2019-02-14T15:50:37Z2017-11info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://hdl.handle.net/20.500.12123/4444https://www.sciencedirect.com/science/article/pii/S0022175917302144?via%3Dihub0022-1759https://doi.org/10.1016/j.jim.2017.07.001Journal of immunological methods 450 : 1-9. (November 2017)reponame:INTA Digital (INTA)instname:Instituto Nacional de Tecnología Agropecuariaenginfo:eu-repo/semantics/restrictedAccess2025-10-23T11:16:50Zoai:localhost:20.500.12123/4444instacron:INTAInstitucionalhttp://repositorio.inta.gob.ar/Organismo científico-tecnológicoNo correspondehttp://repositorio.inta.gob.ar/oai/requesttripaldi.nicolas@inta.gob.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:l2025-10-23 11:16:50.691INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuariafalse
dc.title.none.fl_str_mv Enhanced sensitivity in detection of antiviral antibody responses using biotinylation of foot-and-mouth disease virus (FMDV) capsids
title Enhanced sensitivity in detection of antiviral antibody responses using biotinylation of foot-and-mouth disease virus (FMDV) capsids
spellingShingle Enhanced sensitivity in detection of antiviral antibody responses using biotinylation of foot-and-mouth disease virus (FMDV) capsids
Kenney, Mary
Virus Fiebre Aftosa
Respuesta Inmunológica
ELISA
Citometría de Flujo (Células)
Aphthovirus
Immune Response
Flow Cytometry
Foot and Mouth Disease Virus
Biotinylation
Biotinilación
title_short Enhanced sensitivity in detection of antiviral antibody responses using biotinylation of foot-and-mouth disease virus (FMDV) capsids
title_full Enhanced sensitivity in detection of antiviral antibody responses using biotinylation of foot-and-mouth disease virus (FMDV) capsids
title_fullStr Enhanced sensitivity in detection of antiviral antibody responses using biotinylation of foot-and-mouth disease virus (FMDV) capsids
title_full_unstemmed Enhanced sensitivity in detection of antiviral antibody responses using biotinylation of foot-and-mouth disease virus (FMDV) capsids
title_sort Enhanced sensitivity in detection of antiviral antibody responses using biotinylation of foot-and-mouth disease virus (FMDV) capsids
dc.creator.none.fl_str_mv Kenney, Mary
Waters, Ryan A.
Rieder, Elizabeth
Pega, Juan Franco
Perez Filgueira, Daniel Mariano
Golde, William T.
author Kenney, Mary
author_facet Kenney, Mary
Waters, Ryan A.
Rieder, Elizabeth
Pega, Juan Franco
Perez Filgueira, Daniel Mariano
Golde, William T.
author_role author
author2 Waters, Ryan A.
Rieder, Elizabeth
Pega, Juan Franco
Perez Filgueira, Daniel Mariano
Golde, William T.
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv Virus Fiebre Aftosa
Respuesta Inmunológica
ELISA
Citometría de Flujo (Células)
Aphthovirus
Immune Response
Flow Cytometry
Foot and Mouth Disease Virus
Biotinylation
Biotinilación
topic Virus Fiebre Aftosa
Respuesta Inmunológica
ELISA
Citometría de Flujo (Células)
Aphthovirus
Immune Response
Flow Cytometry
Foot and Mouth Disease Virus
Biotinylation
Biotinilación
dc.description.none.fl_txt_mv Analysis of the immune response to infection of livestock by foot-and-mouth disease virus (FMDV) is most often reported as the serum antibody response to the virus. While measurement of neutralizing antibody has been sensitive and specific, measurements of the quality of the antibody response are less robust. Determining the immunoglobulin (Ig) isotype of the serum antibody response provides a deeper understanding of the biology of the response and more sensitive methods for these assays will facilitate analyses of B cell mediated immunity. We tested the hypothesis that using the virus as the molecular probe could be achieved by adding tags to the surface of the FMDV capsid, and that would enhance sensitivity in assays for anti-FMDV antibody responses. The use of a FLAG-tagged virus in these assays failed to yield improvement whereas chemically biotinylating the virus capsid resulted in significant enhancement of the signal. Here we describe methods using biotinylated virus for measuring anti-viral antibody in serum and antibody secreting cells (ASCs) in blood that are sensitive and specific. Finally, we describe using the biotinylated virus in flow cytometry where such assays should greatly enhance the analysis of anti-virus antibody producing B cells, allowing the investigator to focus on only the FMDV specific B cells when analyzing the development of the B cell response to either infection or vaccination.
Instituto de Virología
Fil: Kenney, Mary. United States Department of Agriculture. Agricultural Research Service. Plum Island Animal Disease Center; Estados Unidos
Fil: Waters, Ryan A. United States Department of Agriculture. Agricultural Research Service. Plum Island Animal Disease Center; Estados Unidos
Fil: Rieder, Elizabeth. United States Department of Agriculture. Agricultural Research Service. Plum Island Animal Disease Center; Estados Unidos
Fil: Pega, Juan Franco. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Perez Filgueira, Daniel Mariano. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Golde, William T. United States Department of Agriculture. Agricultural Research Service. Plum Island Animal Disease Center; Estados Unidos
description Analysis of the immune response to infection of livestock by foot-and-mouth disease virus (FMDV) is most often reported as the serum antibody response to the virus. While measurement of neutralizing antibody has been sensitive and specific, measurements of the quality of the antibody response are less robust. Determining the immunoglobulin (Ig) isotype of the serum antibody response provides a deeper understanding of the biology of the response and more sensitive methods for these assays will facilitate analyses of B cell mediated immunity. We tested the hypothesis that using the virus as the molecular probe could be achieved by adding tags to the surface of the FMDV capsid, and that would enhance sensitivity in assays for anti-FMDV antibody responses. The use of a FLAG-tagged virus in these assays failed to yield improvement whereas chemically biotinylating the virus capsid resulted in significant enhancement of the signal. Here we describe methods using biotinylated virus for measuring anti-viral antibody in serum and antibody secreting cells (ASCs) in blood that are sensitive and specific. Finally, we describe using the biotinylated virus in flow cytometry where such assays should greatly enhance the analysis of anti-virus antibody producing B cells, allowing the investigator to focus on only the FMDV specific B cells when analyzing the development of the B cell response to either infection or vaccination.
publishDate 2017
dc.date.none.fl_str_mv 2017-11
2019-02-14T15:50:37Z
2019-02-14T15:50:37Z
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/20.500.12123/4444
https://www.sciencedirect.com/science/article/pii/S0022175917302144?via%3Dihub
0022-1759
https://doi.org/10.1016/j.jim.2017.07.001
url http://hdl.handle.net/20.500.12123/4444
https://www.sciencedirect.com/science/article/pii/S0022175917302144?via%3Dihub
https://doi.org/10.1016/j.jim.2017.07.001
identifier_str_mv 0022-1759
dc.language.none.fl_str_mv eng
language eng
dc.rights.none.fl_str_mv info:eu-repo/semantics/restrictedAccess
eu_rights_str_mv restrictedAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv Journal of immunological methods 450 : 1-9. (November 2017)
reponame:INTA Digital (INTA)
instname:Instituto Nacional de Tecnología Agropecuaria
reponame_str INTA Digital (INTA)
collection INTA Digital (INTA)
instname_str Instituto Nacional de Tecnología Agropecuaria
repository.name.fl_str_mv INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuaria
repository.mail.fl_str_mv tripaldi.nicolas@inta.gob.ar
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score 12.982451

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