Molecular genotyping of Theileria spp. detected in horses from Corrientes City, Argentina

Autores
Benitez Ibalo, Alicia P.; Debárbora, Valeria Natalia; Mangold, Atilio Jose; Nava, Santiago; Sebastian, Patrick
Año de publicación
2024
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
After the previous description and characterization of Theileria equi sensu stricto (Genotype A) in Argentina, the aim of the present study was to investigate the possible heterogeneity of 18 S rDNA genotypes circulating in this country. Therefore, blood samples from 18 horses from two different neighborhoods of Corrientes City, Corrientes Province, were analyzed for infection with Theileria species. Theileria DNA was detected in five samples (27.8%). Four nearly complete 18 S rDNA gene sequences were obtained and phylogenetic analyses were carried out. The maximum likelihood tree constructed in this study revealed that two different 18 S rDNA genotypes - namely Genotype A (T. equi sensu stricto) and Genotype C (T. equi sensu lato/Theileria haneyi) - are circulating in the same horse population. These results represent to first evidence of T. equi genotype heterogeneity in Argentina and also the first detection of a member of the T. haneyi/Genotype C group. Together with reports from Brazil, the results of this study reinforce the assumption that Genotypes A and C are the predominant genotypes in South America.
Instituto Nacional de Tecnología Agropecuaria, Asociación Cooperadora INTA Rafaela, CONICET (Consejo Nacional de Investigaciones Científicas y Técnicas), Agencia Nacional de Promoción Científica y Tecnológica (PICT 2019 − 970; PICT 2020 − 130) y Secretaría General de Ciencia y Técnica- Universidad Nacional del Nordeste (UNNE PI 21F018).
EEA Rafaela
Fil: Benitez Ibalo, Alicia P. Universidad Nacional del Nordeste. Facultad de Ciencias Exactas y Naturales y Agrimensura. Laboratorio Biología de los Parásitos; Argentina
Fil: Benitez Ibalo, Alicia P. Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET); Argentina
Fil: Debarbora, Valeria Natalia. Universidad Nacional del Nordeste. Facultad de Ciencias Exactas y Naturales y Agrimensura. Laboratorio Biología de los Parásitos; Argentina
Fil: Debarbora, Valeria Natalia. Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET); Argentina
Fil: Mangold, Atilio Jose. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rafaela. Instituto de Investigación de la Cadena Láctea (IDICAL); Argentina
Fil: Mangold, Atilio Jose. Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET). Instituto de Investigación de la Cadena Láctea (IDICAL); Argentina
Fil: Nava, Santiago. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rafaela. Instituto de Investigación de la Cadena Láctea (IDICAL); Argentina
Fil: Nava, Santiago. Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET). Instituto de Investigación de la Cadena Láctea (IDICAL); Argentina
Fil: Sebastian, Patrick. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rafaela. Instituto de Investigación de la Cadena Láctea (IDICAL); Argentina
Fil: Sebastian, Patrick. Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET). Instituto de Investigación de la Cadena Láctea (IDICAL); Argentina
Fuente
Veterinary Research Communications 49: 54 (Published: 24 December 2024)
Materia
Apicomplexa
Piroplasmosis
Filogenia
Genotipos
América del Sur
Theileria
Phylogeny
Genotypes
South America
Equine Piroplasmosis
Piroplasmosis Equina
Corrientes, Argentina
Nivel de accesibilidad
acceso restringido
Condiciones de uso
http://creativecommons.org/licenses/by-nc-sa/4.0/
Repositorio
INTA Digital (INTA)
Institución
Instituto Nacional de Tecnología Agropecuaria
OAI Identificador
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spelling Molecular genotyping of Theileria spp. detected in horses from Corrientes City, ArgentinaBenitez Ibalo, Alicia P.Debárbora, Valeria NataliaMangold, Atilio JoseNava, SantiagoSebastian, PatrickApicomplexaPiroplasmosisFilogeniaGenotiposAmérica del SurTheileriaPhylogenyGenotypesSouth AmericaEquine PiroplasmosisPiroplasmosis EquinaCorrientes, ArgentinaAfter the previous description and characterization of Theileria equi sensu stricto (Genotype A) in Argentina, the aim of the present study was to investigate the possible heterogeneity of 18 S rDNA genotypes circulating in this country. Therefore, blood samples from 18 horses from two different neighborhoods of Corrientes City, Corrientes Province, were analyzed for infection with Theileria species. Theileria DNA was detected in five samples (27.8%). Four nearly complete 18 S rDNA gene sequences were obtained and phylogenetic analyses were carried out. The maximum likelihood tree constructed in this study revealed that two different 18 S rDNA genotypes - namely Genotype A (T. equi sensu stricto) and Genotype C (T. equi sensu lato/Theileria haneyi) - are circulating in the same horse population. These results represent to first evidence of T. equi genotype heterogeneity in Argentina and also the first detection of a member of the T. haneyi/Genotype C group. Together with reports from Brazil, the results of this study reinforce the assumption that Genotypes A and C are the predominant genotypes in South America.Instituto Nacional de Tecnología Agropecuaria, Asociación Cooperadora INTA Rafaela, CONICET (Consejo Nacional de Investigaciones Científicas y Técnicas), Agencia Nacional de Promoción Científica y Tecnológica (PICT 2019 − 970; PICT 2020 − 130) y Secretaría General de Ciencia y Técnica- Universidad Nacional del Nordeste (UNNE PI 21F018).EEA RafaelaFil: Benitez Ibalo, Alicia P. Universidad Nacional del Nordeste. Facultad de Ciencias Exactas y Naturales y Agrimensura. Laboratorio Biología de los Parásitos; ArgentinaFil: Benitez Ibalo, Alicia P. Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET); ArgentinaFil: Debarbora, Valeria Natalia. Universidad Nacional del Nordeste. Facultad de Ciencias Exactas y Naturales y Agrimensura. Laboratorio Biología de los Parásitos; ArgentinaFil: Debarbora, Valeria Natalia. Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET); ArgentinaFil: Mangold, Atilio Jose. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rafaela. Instituto de Investigación de la Cadena Láctea (IDICAL); ArgentinaFil: Mangold, Atilio Jose. Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET). Instituto de Investigación de la Cadena Láctea (IDICAL); ArgentinaFil: Nava, Santiago. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rafaela. Instituto de Investigación de la Cadena Láctea (IDICAL); ArgentinaFil: Nava, Santiago. Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET). Instituto de Investigación de la Cadena Láctea (IDICAL); ArgentinaFil: Sebastian, Patrick. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rafaela. Instituto de Investigación de la Cadena Láctea (IDICAL); ArgentinaFil: Sebastian, Patrick. Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET). Instituto de Investigación de la Cadena Láctea (IDICAL); ArgentinaSpringer2025-01-23T16:44:47Z2025-01-23T16:44:47Z2024-12-24info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://hdl.handle.net/20.500.12123/21057https://link.springer.com/article/10.1007/s11259-024-10618-3Benitez-Ibalo, A.P., Debárbora, V.N., Mangold, A.J. et al. Molecular genotyping of Theileria spp. detected in horses from Corrientes City, Argentina. Vet Res Commun 49, 54 (2025). https://doi.org/10.1007/s11259-024-10618-31573-7446 (electronic)0165-7380 (print)https://doi.org/10.1007/s11259-024-10618-3Veterinary Research Communications 49: 54 (Published: 24 December 2024)reponame:INTA Digital (INTA)instname:Instituto Nacional de Tecnología Agropecuariaenginfo:eu-repograntAgreement/INTA/2019-PE-E5-I109-001/2019-PE-E5-I109-001/AR./Convocatoria: Estudios para el control de enfermedades subtropicales y/o transmitidas por vectores (Tristeza Bovina, Garrapatas, Miasis, Tripanosomiasis, Lengua Azul y laCorrientes .......... (province) (World, South America, Argentina)1001210info:eu-repo/semantics/restrictedAccesshttp://creativecommons.org/licenses/by-nc-sa/4.0/Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)2025-10-23T11:19:21Zoai:localhost:20.500.12123/21057instacron:INTAInstitucionalhttp://repositorio.inta.gob.ar/Organismo científico-tecnológicoNo correspondehttp://repositorio.inta.gob.ar/oai/requesttripaldi.nicolas@inta.gob.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:l2025-10-23 11:19:21.809INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuariafalse
dc.title.none.fl_str_mv Molecular genotyping of Theileria spp. detected in horses from Corrientes City, Argentina
title Molecular genotyping of Theileria spp. detected in horses from Corrientes City, Argentina
spellingShingle Molecular genotyping of Theileria spp. detected in horses from Corrientes City, Argentina
Benitez Ibalo, Alicia P.
Apicomplexa
Piroplasmosis
Filogenia
Genotipos
América del Sur
Theileria
Phylogeny
Genotypes
South America
Equine Piroplasmosis
Piroplasmosis Equina
Corrientes, Argentina
title_short Molecular genotyping of Theileria spp. detected in horses from Corrientes City, Argentina
title_full Molecular genotyping of Theileria spp. detected in horses from Corrientes City, Argentina
title_fullStr Molecular genotyping of Theileria spp. detected in horses from Corrientes City, Argentina
title_full_unstemmed Molecular genotyping of Theileria spp. detected in horses from Corrientes City, Argentina
title_sort Molecular genotyping of Theileria spp. detected in horses from Corrientes City, Argentina
dc.creator.none.fl_str_mv Benitez Ibalo, Alicia P.
Debárbora, Valeria Natalia
Mangold, Atilio Jose
Nava, Santiago
Sebastian, Patrick
author Benitez Ibalo, Alicia P.
author_facet Benitez Ibalo, Alicia P.
Debárbora, Valeria Natalia
Mangold, Atilio Jose
Nava, Santiago
Sebastian, Patrick
author_role author
author2 Debárbora, Valeria Natalia
Mangold, Atilio Jose
Nava, Santiago
Sebastian, Patrick
author2_role author
author
author
author
dc.subject.none.fl_str_mv Apicomplexa
Piroplasmosis
Filogenia
Genotipos
América del Sur
Theileria
Phylogeny
Genotypes
South America
Equine Piroplasmosis
Piroplasmosis Equina
Corrientes, Argentina
topic Apicomplexa
Piroplasmosis
Filogenia
Genotipos
América del Sur
Theileria
Phylogeny
Genotypes
South America
Equine Piroplasmosis
Piroplasmosis Equina
Corrientes, Argentina
dc.description.none.fl_txt_mv After the previous description and characterization of Theileria equi sensu stricto (Genotype A) in Argentina, the aim of the present study was to investigate the possible heterogeneity of 18 S rDNA genotypes circulating in this country. Therefore, blood samples from 18 horses from two different neighborhoods of Corrientes City, Corrientes Province, were analyzed for infection with Theileria species. Theileria DNA was detected in five samples (27.8%). Four nearly complete 18 S rDNA gene sequences were obtained and phylogenetic analyses were carried out. The maximum likelihood tree constructed in this study revealed that two different 18 S rDNA genotypes - namely Genotype A (T. equi sensu stricto) and Genotype C (T. equi sensu lato/Theileria haneyi) - are circulating in the same horse population. These results represent to first evidence of T. equi genotype heterogeneity in Argentina and also the first detection of a member of the T. haneyi/Genotype C group. Together with reports from Brazil, the results of this study reinforce the assumption that Genotypes A and C are the predominant genotypes in South America.
Instituto Nacional de Tecnología Agropecuaria, Asociación Cooperadora INTA Rafaela, CONICET (Consejo Nacional de Investigaciones Científicas y Técnicas), Agencia Nacional de Promoción Científica y Tecnológica (PICT 2019 − 970; PICT 2020 − 130) y Secretaría General de Ciencia y Técnica- Universidad Nacional del Nordeste (UNNE PI 21F018).
EEA Rafaela
Fil: Benitez Ibalo, Alicia P. Universidad Nacional del Nordeste. Facultad de Ciencias Exactas y Naturales y Agrimensura. Laboratorio Biología de los Parásitos; Argentina
Fil: Benitez Ibalo, Alicia P. Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET); Argentina
Fil: Debarbora, Valeria Natalia. Universidad Nacional del Nordeste. Facultad de Ciencias Exactas y Naturales y Agrimensura. Laboratorio Biología de los Parásitos; Argentina
Fil: Debarbora, Valeria Natalia. Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET); Argentina
Fil: Mangold, Atilio Jose. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rafaela. Instituto de Investigación de la Cadena Láctea (IDICAL); Argentina
Fil: Mangold, Atilio Jose. Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET). Instituto de Investigación de la Cadena Láctea (IDICAL); Argentina
Fil: Nava, Santiago. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rafaela. Instituto de Investigación de la Cadena Láctea (IDICAL); Argentina
Fil: Nava, Santiago. Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET). Instituto de Investigación de la Cadena Láctea (IDICAL); Argentina
Fil: Sebastian, Patrick. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rafaela. Instituto de Investigación de la Cadena Láctea (IDICAL); Argentina
Fil: Sebastian, Patrick. Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET). Instituto de Investigación de la Cadena Láctea (IDICAL); Argentina
description After the previous description and characterization of Theileria equi sensu stricto (Genotype A) in Argentina, the aim of the present study was to investigate the possible heterogeneity of 18 S rDNA genotypes circulating in this country. Therefore, blood samples from 18 horses from two different neighborhoods of Corrientes City, Corrientes Province, were analyzed for infection with Theileria species. Theileria DNA was detected in five samples (27.8%). Four nearly complete 18 S rDNA gene sequences were obtained and phylogenetic analyses were carried out. The maximum likelihood tree constructed in this study revealed that two different 18 S rDNA genotypes - namely Genotype A (T. equi sensu stricto) and Genotype C (T. equi sensu lato/Theileria haneyi) - are circulating in the same horse population. These results represent to first evidence of T. equi genotype heterogeneity in Argentina and also the first detection of a member of the T. haneyi/Genotype C group. Together with reports from Brazil, the results of this study reinforce the assumption that Genotypes A and C are the predominant genotypes in South America.
publishDate 2024
dc.date.none.fl_str_mv 2024-12-24
2025-01-23T16:44:47Z
2025-01-23T16:44:47Z
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/20.500.12123/21057
https://link.springer.com/article/10.1007/s11259-024-10618-3
Benitez-Ibalo, A.P., Debárbora, V.N., Mangold, A.J. et al. Molecular genotyping of Theileria spp. detected in horses from Corrientes City, Argentina. Vet Res Commun 49, 54 (2025). https://doi.org/10.1007/s11259-024-10618-3
1573-7446 (electronic)
0165-7380 (print)
https://doi.org/10.1007/s11259-024-10618-3
url http://hdl.handle.net/20.500.12123/21057
https://link.springer.com/article/10.1007/s11259-024-10618-3
https://doi.org/10.1007/s11259-024-10618-3
identifier_str_mv Benitez-Ibalo, A.P., Debárbora, V.N., Mangold, A.J. et al. Molecular genotyping of Theileria spp. detected in horses from Corrientes City, Argentina. Vet Res Commun 49, 54 (2025). https://doi.org/10.1007/s11259-024-10618-3
1573-7446 (electronic)
0165-7380 (print)
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repograntAgreement/INTA/2019-PE-E5-I109-001/2019-PE-E5-I109-001/AR./Convocatoria: Estudios para el control de enfermedades subtropicales y/o transmitidas por vectores (Tristeza Bovina, Garrapatas, Miasis, Tripanosomiasis, Lengua Azul y la
dc.rights.none.fl_str_mv info:eu-repo/semantics/restrictedAccess
http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
eu_rights_str_mv restrictedAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
dc.format.none.fl_str_mv application/pdf
dc.coverage.none.fl_str_mv Corrientes .......... (province) (World, South America, Argentina)
1001210
dc.publisher.none.fl_str_mv Springer
publisher.none.fl_str_mv Springer
dc.source.none.fl_str_mv Veterinary Research Communications 49: 54 (Published: 24 December 2024)
reponame:INTA Digital (INTA)
instname:Instituto Nacional de Tecnología Agropecuaria
reponame_str INTA Digital (INTA)
collection INTA Digital (INTA)
instname_str Instituto Nacional de Tecnología Agropecuaria
repository.name.fl_str_mv INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuaria
repository.mail.fl_str_mv tripaldi.nicolas@inta.gob.ar
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