Before-after analysis of genetic and epigenetic markers in garlic: A 13-year experiment

Autores
Gimenez, Magali Diana; Garcia Lampasona, Sandra Claudia
Año de publicación
2018
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Allium sativum is an important crop species in Argentina, one of the major garlic exporting countries in the world. Because garlic is an agamic propagated species, its breeding is based on the occurrence of plants with desirable traits, which are attributed to genetic mutations. In this work, we took advantage of a long term field experiment in a garlic line destined to genetic improvement to evaluate bulb weight and genetic and epigenetic stability and dynamics over 13 years. Average bulb weight increased from 65.3 g in the first year to 84.8 g the 13th year. Using AFLP (Amplified Fragment Length Polymorphism) and MSAP (Methylation Sensitive Amplified Polymorphism) techniques, we assessed genetic and epigenetic stability in plants of A. sativum taken at first, third and 13th year of field culture. We detected that 82.47 and 19.61% of the AFLP and MSAP fragments were present in the tree years of sampling. The application of selection pressure led to a reduction in genetic polymorphism and an increase of bulb weight, but did not influence epigenetic polymorphism, indicating that it is independent of genetic variability. We detected changes in the amount of each methylation pattern (unmethylated, hemimethylated and internally methylated) among the different years analyzed while 82.47 and 19.61% of the genetic and epigenetical loci were stable during the time of culture. Although some epiloci showed stability along the 13 years of culture, others presented gradual variation, while others were polymorphic within samples from the same year of culture. Finally, we discussed the implications of the high epigenetic variability of an agamic propagated species and its possible effect on the phenotype.
EEA Mendoza
Fil: Gimenez, Magali Diana. Universidad Nacional de Cuyo. Facultad de Ciencias Agrarias. Laboratorio de Biologia Molecular; Argentina; Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Mendoza; Argentina
Fil: Garcia Lampasona, Sandra Claudia. Universidad Nacional de Cuyo. Facultad de Ciencias Agrarias. Laboratorio de Biología Molecular; Argentina; Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Mendoza; Argentina. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Mendoza; Argentina
Fuente
Scientia Horticulturae 240 : 23-28 (October 2018)
Materia
Allium Sativum
Ajo
Marcadores Genéticos
Epigenético
Estabilidad Genética
Garlic
Genetic Markers
Epigenetics
Genetic Stability
Argentina
Nivel de accesibilidad
acceso restringido
Condiciones de uso
Repositorio
INTA Digital (INTA)
Institución
Instituto Nacional de Tecnología Agropecuaria
OAI Identificador
oai:localhost:20.500.12123/2660
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network_name_str INTA Digital (INTA)
spelling Before-after analysis of genetic and epigenetic markers in garlic: A 13-year experimentGimenez, Magali DianaGarcia Lampasona, Sandra ClaudiaAllium SativumAjoMarcadores GenéticosEpigenéticoEstabilidad GenéticaGarlicGenetic MarkersEpigeneticsGenetic StabilityArgentinaAllium sativum is an important crop species in Argentina, one of the major garlic exporting countries in the world. Because garlic is an agamic propagated species, its breeding is based on the occurrence of plants with desirable traits, which are attributed to genetic mutations. In this work, we took advantage of a long term field experiment in a garlic line destined to genetic improvement to evaluate bulb weight and genetic and epigenetic stability and dynamics over 13 years. Average bulb weight increased from 65.3 g in the first year to 84.8 g the 13th year. Using AFLP (Amplified Fragment Length Polymorphism) and MSAP (Methylation Sensitive Amplified Polymorphism) techniques, we assessed genetic and epigenetic stability in plants of A. sativum taken at first, third and 13th year of field culture. We detected that 82.47 and 19.61% of the AFLP and MSAP fragments were present in the tree years of sampling. The application of selection pressure led to a reduction in genetic polymorphism and an increase of bulb weight, but did not influence epigenetic polymorphism, indicating that it is independent of genetic variability. We detected changes in the amount of each methylation pattern (unmethylated, hemimethylated and internally methylated) among the different years analyzed while 82.47 and 19.61% of the genetic and epigenetical loci were stable during the time of culture. Although some epiloci showed stability along the 13 years of culture, others presented gradual variation, while others were polymorphic within samples from the same year of culture. Finally, we discussed the implications of the high epigenetic variability of an agamic propagated species and its possible effect on the phenotype.EEA MendozaFil: Gimenez, Magali Diana. Universidad Nacional de Cuyo. Facultad de Ciencias Agrarias. Laboratorio de Biologia Molecular; Argentina; Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Mendoza; ArgentinaFil: Garcia Lampasona, Sandra Claudia. Universidad Nacional de Cuyo. Facultad de Ciencias Agrarias. Laboratorio de Biología Molecular; Argentina; Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Mendoza; Argentina. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Mendoza; Argentina2018-06-19T14:26:50Z2018-06-19T14:26:50Z2018-10-20info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttps://www.sciencedirect.com/science/article/pii/S0304423818302930http://hdl.handle.net/20.500.12123/26600304-4238https://doi.org/10.1016/j.scienta.2018.04.044Scientia Horticulturae 240 : 23-28 (October 2018)reponame:INTA Digital (INTA)instname:Instituto Nacional de Tecnología Agropecuariaenginfo:eu-repo/semantics/restrictedAccess2025-09-11T10:22:25Zoai:localhost:20.500.12123/2660instacron:INTAInstitucionalhttp://repositorio.inta.gob.ar/Organismo científico-tecnológicoNo correspondehttp://repositorio.inta.gob.ar/oai/requesttripaldi.nicolas@inta.gob.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:l2025-09-11 10:22:25.976INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuariafalse
dc.title.none.fl_str_mv Before-after analysis of genetic and epigenetic markers in garlic: A 13-year experiment
title Before-after analysis of genetic and epigenetic markers in garlic: A 13-year experiment
spellingShingle Before-after analysis of genetic and epigenetic markers in garlic: A 13-year experiment
Gimenez, Magali Diana
Allium Sativum
Ajo
Marcadores Genéticos
Epigenético
Estabilidad Genética
Garlic
Genetic Markers
Epigenetics
Genetic Stability
Argentina
title_short Before-after analysis of genetic and epigenetic markers in garlic: A 13-year experiment
title_full Before-after analysis of genetic and epigenetic markers in garlic: A 13-year experiment
title_fullStr Before-after analysis of genetic and epigenetic markers in garlic: A 13-year experiment
title_full_unstemmed Before-after analysis of genetic and epigenetic markers in garlic: A 13-year experiment
title_sort Before-after analysis of genetic and epigenetic markers in garlic: A 13-year experiment
dc.creator.none.fl_str_mv Gimenez, Magali Diana
Garcia Lampasona, Sandra Claudia
author Gimenez, Magali Diana
author_facet Gimenez, Magali Diana
Garcia Lampasona, Sandra Claudia
author_role author
author2 Garcia Lampasona, Sandra Claudia
author2_role author
dc.subject.none.fl_str_mv Allium Sativum
Ajo
Marcadores Genéticos
Epigenético
Estabilidad Genética
Garlic
Genetic Markers
Epigenetics
Genetic Stability
Argentina
topic Allium Sativum
Ajo
Marcadores Genéticos
Epigenético
Estabilidad Genética
Garlic
Genetic Markers
Epigenetics
Genetic Stability
Argentina
dc.description.none.fl_txt_mv Allium sativum is an important crop species in Argentina, one of the major garlic exporting countries in the world. Because garlic is an agamic propagated species, its breeding is based on the occurrence of plants with desirable traits, which are attributed to genetic mutations. In this work, we took advantage of a long term field experiment in a garlic line destined to genetic improvement to evaluate bulb weight and genetic and epigenetic stability and dynamics over 13 years. Average bulb weight increased from 65.3 g in the first year to 84.8 g the 13th year. Using AFLP (Amplified Fragment Length Polymorphism) and MSAP (Methylation Sensitive Amplified Polymorphism) techniques, we assessed genetic and epigenetic stability in plants of A. sativum taken at first, third and 13th year of field culture. We detected that 82.47 and 19.61% of the AFLP and MSAP fragments were present in the tree years of sampling. The application of selection pressure led to a reduction in genetic polymorphism and an increase of bulb weight, but did not influence epigenetic polymorphism, indicating that it is independent of genetic variability. We detected changes in the amount of each methylation pattern (unmethylated, hemimethylated and internally methylated) among the different years analyzed while 82.47 and 19.61% of the genetic and epigenetical loci were stable during the time of culture. Although some epiloci showed stability along the 13 years of culture, others presented gradual variation, while others were polymorphic within samples from the same year of culture. Finally, we discussed the implications of the high epigenetic variability of an agamic propagated species and its possible effect on the phenotype.
EEA Mendoza
Fil: Gimenez, Magali Diana. Universidad Nacional de Cuyo. Facultad de Ciencias Agrarias. Laboratorio de Biologia Molecular; Argentina; Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Mendoza; Argentina
Fil: Garcia Lampasona, Sandra Claudia. Universidad Nacional de Cuyo. Facultad de Ciencias Agrarias. Laboratorio de Biología Molecular; Argentina; Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Mendoza; Argentina. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Mendoza; Argentina
description Allium sativum is an important crop species in Argentina, one of the major garlic exporting countries in the world. Because garlic is an agamic propagated species, its breeding is based on the occurrence of plants with desirable traits, which are attributed to genetic mutations. In this work, we took advantage of a long term field experiment in a garlic line destined to genetic improvement to evaluate bulb weight and genetic and epigenetic stability and dynamics over 13 years. Average bulb weight increased from 65.3 g in the first year to 84.8 g the 13th year. Using AFLP (Amplified Fragment Length Polymorphism) and MSAP (Methylation Sensitive Amplified Polymorphism) techniques, we assessed genetic and epigenetic stability in plants of A. sativum taken at first, third and 13th year of field culture. We detected that 82.47 and 19.61% of the AFLP and MSAP fragments were present in the tree years of sampling. The application of selection pressure led to a reduction in genetic polymorphism and an increase of bulb weight, but did not influence epigenetic polymorphism, indicating that it is independent of genetic variability. We detected changes in the amount of each methylation pattern (unmethylated, hemimethylated and internally methylated) among the different years analyzed while 82.47 and 19.61% of the genetic and epigenetical loci were stable during the time of culture. Although some epiloci showed stability along the 13 years of culture, others presented gradual variation, while others were polymorphic within samples from the same year of culture. Finally, we discussed the implications of the high epigenetic variability of an agamic propagated species and its possible effect on the phenotype.
publishDate 2018
dc.date.none.fl_str_mv 2018-06-19T14:26:50Z
2018-06-19T14:26:50Z
2018-10-20
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv https://www.sciencedirect.com/science/article/pii/S0304423818302930
http://hdl.handle.net/20.500.12123/2660
0304-4238
https://doi.org/10.1016/j.scienta.2018.04.044
url https://www.sciencedirect.com/science/article/pii/S0304423818302930
http://hdl.handle.net/20.500.12123/2660
https://doi.org/10.1016/j.scienta.2018.04.044
identifier_str_mv 0304-4238
dc.language.none.fl_str_mv eng
language eng
dc.rights.none.fl_str_mv info:eu-repo/semantics/restrictedAccess
eu_rights_str_mv restrictedAccess
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv Scientia Horticulturae 240 : 23-28 (October 2018)
reponame:INTA Digital (INTA)
instname:Instituto Nacional de Tecnología Agropecuaria
reponame_str INTA Digital (INTA)
collection INTA Digital (INTA)
instname_str Instituto Nacional de Tecnología Agropecuaria
repository.name.fl_str_mv INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuaria
repository.mail.fl_str_mv tripaldi.nicolas@inta.gob.ar
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