Assessment of genetic diversity and population structure in a garlic (Allium sativum L.) germplasm collection varying in bulb content of pyruvate, phenolics, and solids

Autores
Barboza Rojas, Karina; Salinas Palmieri, Maria Cecilia; Acuña, Cintia Vanesa; Bannoud, Florencia; Beretta, Hebe Vanesa; Garcia Lampasona, Sandra Claudia; Burba, Jose Luis; Galmarini, Claudio Romulo; Cavagnaro, Pablo
Año de publicación
2019
Idioma
inglés
Tipo de recurso
artículo
Estado
versión aceptada
Descripción
This study characterized genetic diversity in the Argentine garlic germplasm bank by analysis of 10 SSR loci in 73 garlic accessions. A total of 43 alleles and polymorphic information content (PIC) value of 0.38, along with various accessions that could not be differentiated with these SSR markers, suggest a relatively high level of genetic redundancy in this garlic collection. Nonetheless, cluster analysis using different methodologies (UPGMA, STRUCTURE, and PCoA) consistently and clearly separated these germplasm into four clusters. The garlic accessions were clustered in general agreement with their flowering behavior, botanical variety, ecophysiological group, and commercial bulb color-type. Two SSR markers strongly associated with the flowering phenotype (i.e., bolting vs. non-bolting) and ecophysiological group of the accessions were identified. In addition to the genetic analysis, the garlic accessions were characterized for their bulb content of solids, total organosulfur compounds (estimated by analysis of pyruvate levels) and total phenolic compounds, three groups of compounds associated with garlic’s flavor (pungency), nutraceutical value, and postharvest quality. Significant (p < 0.001) and broad variation was found among the accessions for all three phytochemicals, and garlic genotypes with high levels of these compounds were identified. These results suggest that it will be possible to select for mild and pungent garlics, as well as for garlics with high functional value and longer shelf-life. Significant variation was revealed for phytochemicals content among different classes of botanical variety, flowering behavior and ecophysiological group.
EEA La Consulta
Fil: Barboza Rojas, Karina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Mendoza; Argentina. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria La Consulta; Argentina
Fil: Salinas Palmieri, Maria Cecilia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Mendoza; Argentina. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria La Consulta; Argentina
Fil: Acuña, Cintia Vanesa. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina
Fil: Bannoud, Florencia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Mendoza; Argentina. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria La Consulta; Argentina
Fil: Beretta, Hebe Vanesa. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Mendoza; Argentina. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria La Consulta; Argentina
Fil: Garcia Lampasona, Sandra Claudia. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria La Consulta; Argentina. Universidad Nacional de Cuyo. Laboratorio de Biología Molecular. Consejo Nacional de Investigaciones Científicas y Técnicas. Laboratorio de Biología Molecular; Argentina
Fil: Burba, José Luis. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria La Consulta; Argentina
Fil: Galmarini, Claudio Romulo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Mendoza; Argentina. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria La Consulta; Argentina. Universidad Nacional de Cuyo. Facultad de Ciencias Agrarias. Instituto de Horticultura; Argentina
Fil: Cavagnaro, Pablo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Mendoza; Argentina. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria La Consulta; Argentina. Universidad Nacional de Cuyo. Facultad de Ciencias Agrarias. Instituto de Horticultura; Argentina
Fuente
Scientia Horticulturae : 108900 (Available online October 2019)
Materia
Ajo
Diversidad Genética (como Recurso)
Germoplasma
Piruvatos
Compuestos Fenólicos
Allium Sativum
Microsatélites
Marcadores Genéticos
Garlic
Genetic Diversity (as Resource)
Germplasm
Pyruvates
Phenolic Compounds
Microsatellites
Genetic Markers
Marcadores Moleculares
Nivel de accesibilidad
acceso restringido
Condiciones de uso
Repositorio
INTA Digital (INTA)
Institución
Instituto Nacional de Tecnología Agropecuaria
OAI Identificador
oai:localhost:20.500.12123/6243
Acceder
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oai_identifier_str oai:localhost:20.500.12123/6243
network_acronym_str INTADig
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network_name_str INTA Digital (INTA)
spelling Assessment of genetic diversity and population structure in a garlic (Allium sativum L.) germplasm collection varying in bulb content of pyruvate, phenolics, and solidsBarboza Rojas, KarinaSalinas Palmieri, Maria CeciliaAcuña, Cintia VanesaBannoud, FlorenciaBeretta, Hebe VanesaGarcia Lampasona, Sandra ClaudiaBurba, Jose LuisGalmarini, Claudio RomuloCavagnaro, PabloAjoDiversidad Genética (como Recurso)GermoplasmaPiruvatosCompuestos FenólicosAllium SativumMicrosatélitesMarcadores GenéticosGarlicGenetic Diversity (as Resource)GermplasmPyruvatesPhenolic CompoundsMicrosatellitesGenetic MarkersMarcadores MolecularesThis study characterized genetic diversity in the Argentine garlic germplasm bank by analysis of 10 SSR loci in 73 garlic accessions. A total of 43 alleles and polymorphic information content (PIC) value of 0.38, along with various accessions that could not be differentiated with these SSR markers, suggest a relatively high level of genetic redundancy in this garlic collection. Nonetheless, cluster analysis using different methodologies (UPGMA, STRUCTURE, and PCoA) consistently and clearly separated these germplasm into four clusters. The garlic accessions were clustered in general agreement with their flowering behavior, botanical variety, ecophysiological group, and commercial bulb color-type. Two SSR markers strongly associated with the flowering phenotype (i.e., bolting vs. non-bolting) and ecophysiological group of the accessions were identified. In addition to the genetic analysis, the garlic accessions were characterized for their bulb content of solids, total organosulfur compounds (estimated by analysis of pyruvate levels) and total phenolic compounds, three groups of compounds associated with garlic’s flavor (pungency), nutraceutical value, and postharvest quality. Significant (p < 0.001) and broad variation was found among the accessions for all three phytochemicals, and garlic genotypes with high levels of these compounds were identified. These results suggest that it will be possible to select for mild and pungent garlics, as well as for garlics with high functional value and longer shelf-life. Significant variation was revealed for phytochemicals content among different classes of botanical variety, flowering behavior and ecophysiological group.EEA La ConsultaFil: Barboza Rojas, Karina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Mendoza; Argentina. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria La Consulta; ArgentinaFil: Salinas Palmieri, Maria Cecilia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Mendoza; Argentina. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria La Consulta; ArgentinaFil: Acuña, Cintia Vanesa. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; ArgentinaFil: Bannoud, Florencia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Mendoza; Argentina. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria La Consulta; ArgentinaFil: Beretta, Hebe Vanesa. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Mendoza; Argentina. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria La Consulta; ArgentinaFil: Garcia Lampasona, Sandra Claudia. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria La Consulta; Argentina. Universidad Nacional de Cuyo. Laboratorio de Biología Molecular. Consejo Nacional de Investigaciones Científicas y Técnicas. Laboratorio de Biología Molecular; ArgentinaFil: Burba, José Luis. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria La Consulta; ArgentinaFil: Galmarini, Claudio Romulo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Mendoza; Argentina. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria La Consulta; Argentina. Universidad Nacional de Cuyo. Facultad de Ciencias Agrarias. Instituto de Horticultura; ArgentinaFil: Cavagnaro, Pablo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Mendoza; Argentina. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria La Consulta; Argentina. Universidad Nacional de Cuyo. Facultad de Ciencias Agrarias. Instituto de Horticultura; ArgentinaElsevier2019-10-30T15:10:30Z2019-10-30T15:10:30Z2019-10info:eu-repo/semantics/articleinfo:eu-repo/semantics/acceptedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttps://www.sciencedirect.com/science/article/pii/S0304423819307861http://hdl.handle.net/20.500.12123/62430304-4238https://doi.org/10.1016/j.scienta.2019.108900Scientia Horticulturae : 108900 (Available online October 2019)reponame:INTA Digital (INTA)instname:Instituto Nacional de Tecnología Agropecuariaenginfo:eu-repo/semantics/restrictedAccess2025-09-29T13:44:48Zoai:localhost:20.500.12123/6243instacron:INTAInstitucionalhttp://repositorio.inta.gob.ar/Organismo científico-tecnológicoNo correspondehttp://repositorio.inta.gob.ar/oai/requesttripaldi.nicolas@inta.gob.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:l2025-09-29 13:44:49.214INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuariafalse
dc.title.none.fl_str_mv Assessment of genetic diversity and population structure in a garlic (Allium sativum L.) germplasm collection varying in bulb content of pyruvate, phenolics, and solids
title Assessment of genetic diversity and population structure in a garlic (Allium sativum L.) germplasm collection varying in bulb content of pyruvate, phenolics, and solids
spellingShingle Assessment of genetic diversity and population structure in a garlic (Allium sativum L.) germplasm collection varying in bulb content of pyruvate, phenolics, and solids
Barboza Rojas, Karina
Ajo
Diversidad Genética (como Recurso)
Germoplasma
Piruvatos
Compuestos Fenólicos
Allium Sativum
Microsatélites
Marcadores Genéticos
Garlic
Genetic Diversity (as Resource)
Germplasm
Pyruvates
Phenolic Compounds
Microsatellites
Genetic Markers
Marcadores Moleculares
title_short Assessment of genetic diversity and population structure in a garlic (Allium sativum L.) germplasm collection varying in bulb content of pyruvate, phenolics, and solids
title_full Assessment of genetic diversity and population structure in a garlic (Allium sativum L.) germplasm collection varying in bulb content of pyruvate, phenolics, and solids
title_fullStr Assessment of genetic diversity and population structure in a garlic (Allium sativum L.) germplasm collection varying in bulb content of pyruvate, phenolics, and solids
title_full_unstemmed Assessment of genetic diversity and population structure in a garlic (Allium sativum L.) germplasm collection varying in bulb content of pyruvate, phenolics, and solids
title_sort Assessment of genetic diversity and population structure in a garlic (Allium sativum L.) germplasm collection varying in bulb content of pyruvate, phenolics, and solids
dc.creator.none.fl_str_mv Barboza Rojas, Karina
Salinas Palmieri, Maria Cecilia
Acuña, Cintia Vanesa
Bannoud, Florencia
Beretta, Hebe Vanesa
Garcia Lampasona, Sandra Claudia
Burba, Jose Luis
Galmarini, Claudio Romulo
Cavagnaro, Pablo
author Barboza Rojas, Karina
author_facet Barboza Rojas, Karina
Salinas Palmieri, Maria Cecilia
Acuña, Cintia Vanesa
Bannoud, Florencia
Beretta, Hebe Vanesa
Garcia Lampasona, Sandra Claudia
Burba, Jose Luis
Galmarini, Claudio Romulo
Cavagnaro, Pablo
author_role author
author2 Salinas Palmieri, Maria Cecilia
Acuña, Cintia Vanesa
Bannoud, Florencia
Beretta, Hebe Vanesa
Garcia Lampasona, Sandra Claudia
Burba, Jose Luis
Galmarini, Claudio Romulo
Cavagnaro, Pablo
author2_role author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Ajo
Diversidad Genética (como Recurso)
Germoplasma
Piruvatos
Compuestos Fenólicos
Allium Sativum
Microsatélites
Marcadores Genéticos
Garlic
Genetic Diversity (as Resource)
Germplasm
Pyruvates
Phenolic Compounds
Microsatellites
Genetic Markers
Marcadores Moleculares
topic Ajo
Diversidad Genética (como Recurso)
Germoplasma
Piruvatos
Compuestos Fenólicos
Allium Sativum
Microsatélites
Marcadores Genéticos
Garlic
Genetic Diversity (as Resource)
Germplasm
Pyruvates
Phenolic Compounds
Microsatellites
Genetic Markers
Marcadores Moleculares
dc.description.none.fl_txt_mv This study characterized genetic diversity in the Argentine garlic germplasm bank by analysis of 10 SSR loci in 73 garlic accessions. A total of 43 alleles and polymorphic information content (PIC) value of 0.38, along with various accessions that could not be differentiated with these SSR markers, suggest a relatively high level of genetic redundancy in this garlic collection. Nonetheless, cluster analysis using different methodologies (UPGMA, STRUCTURE, and PCoA) consistently and clearly separated these germplasm into four clusters. The garlic accessions were clustered in general agreement with their flowering behavior, botanical variety, ecophysiological group, and commercial bulb color-type. Two SSR markers strongly associated with the flowering phenotype (i.e., bolting vs. non-bolting) and ecophysiological group of the accessions were identified. In addition to the genetic analysis, the garlic accessions were characterized for their bulb content of solids, total organosulfur compounds (estimated by analysis of pyruvate levels) and total phenolic compounds, three groups of compounds associated with garlic’s flavor (pungency), nutraceutical value, and postharvest quality. Significant (p < 0.001) and broad variation was found among the accessions for all three phytochemicals, and garlic genotypes with high levels of these compounds were identified. These results suggest that it will be possible to select for mild and pungent garlics, as well as for garlics with high functional value and longer shelf-life. Significant variation was revealed for phytochemicals content among different classes of botanical variety, flowering behavior and ecophysiological group.
EEA La Consulta
Fil: Barboza Rojas, Karina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Mendoza; Argentina. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria La Consulta; Argentina
Fil: Salinas Palmieri, Maria Cecilia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Mendoza; Argentina. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria La Consulta; Argentina
Fil: Acuña, Cintia Vanesa. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina
Fil: Bannoud, Florencia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Mendoza; Argentina. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria La Consulta; Argentina
Fil: Beretta, Hebe Vanesa. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Mendoza; Argentina. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria La Consulta; Argentina
Fil: Garcia Lampasona, Sandra Claudia. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria La Consulta; Argentina. Universidad Nacional de Cuyo. Laboratorio de Biología Molecular. Consejo Nacional de Investigaciones Científicas y Técnicas. Laboratorio de Biología Molecular; Argentina
Fil: Burba, José Luis. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria La Consulta; Argentina
Fil: Galmarini, Claudio Romulo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Mendoza; Argentina. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria La Consulta; Argentina. Universidad Nacional de Cuyo. Facultad de Ciencias Agrarias. Instituto de Horticultura; Argentina
Fil: Cavagnaro, Pablo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Mendoza; Argentina. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria La Consulta; Argentina. Universidad Nacional de Cuyo. Facultad de Ciencias Agrarias. Instituto de Horticultura; Argentina
description This study characterized genetic diversity in the Argentine garlic germplasm bank by analysis of 10 SSR loci in 73 garlic accessions. A total of 43 alleles and polymorphic information content (PIC) value of 0.38, along with various accessions that could not be differentiated with these SSR markers, suggest a relatively high level of genetic redundancy in this garlic collection. Nonetheless, cluster analysis using different methodologies (UPGMA, STRUCTURE, and PCoA) consistently and clearly separated these germplasm into four clusters. The garlic accessions were clustered in general agreement with their flowering behavior, botanical variety, ecophysiological group, and commercial bulb color-type. Two SSR markers strongly associated with the flowering phenotype (i.e., bolting vs. non-bolting) and ecophysiological group of the accessions were identified. In addition to the genetic analysis, the garlic accessions were characterized for their bulb content of solids, total organosulfur compounds (estimated by analysis of pyruvate levels) and total phenolic compounds, three groups of compounds associated with garlic’s flavor (pungency), nutraceutical value, and postharvest quality. Significant (p < 0.001) and broad variation was found among the accessions for all three phytochemicals, and garlic genotypes with high levels of these compounds were identified. These results suggest that it will be possible to select for mild and pungent garlics, as well as for garlics with high functional value and longer shelf-life. Significant variation was revealed for phytochemicals content among different classes of botanical variety, flowering behavior and ecophysiological group.
publishDate 2019
dc.date.none.fl_str_mv 2019-10-30T15:10:30Z
2019-10-30T15:10:30Z
2019-10
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/acceptedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str acceptedVersion
dc.identifier.none.fl_str_mv https://www.sciencedirect.com/science/article/pii/S0304423819307861
http://hdl.handle.net/20.500.12123/6243
0304-4238
https://doi.org/10.1016/j.scienta.2019.108900
url https://www.sciencedirect.com/science/article/pii/S0304423819307861
http://hdl.handle.net/20.500.12123/6243
https://doi.org/10.1016/j.scienta.2019.108900
identifier_str_mv 0304-4238
dc.language.none.fl_str_mv eng
language eng
dc.rights.none.fl_str_mv info:eu-repo/semantics/restrictedAccess
eu_rights_str_mv restrictedAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv Scientia Horticulturae : 108900 (Available online October 2019)
reponame:INTA Digital (INTA)
instname:Instituto Nacional de Tecnología Agropecuaria
reponame_str INTA Digital (INTA)
collection INTA Digital (INTA)
instname_str Instituto Nacional de Tecnología Agropecuaria
repository.name.fl_str_mv INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuaria
repository.mail.fl_str_mv tripaldi.nicolas@inta.gob.ar
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score 12.559606

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