Geographical distribution, genetic studies and vector transmission of alfalfa enamovirus-1 infecting alfalfa crop in Argentina

Autores
Trucco, Veronica Milagros; Cabrera Mederos, Dariel; Lenardon, Sergio Luis; Giolitti, Fabian
Año de publicación
2020
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Alfalfa (Medicago sativa L.) growing areas of Argentina were surveyed between 2010 and 2018 to determine the geographical distribution and analyse the genetic diversity among alfalfa enamovirus-1 (AEV-1) isolates. The virus was detected in all 17 surveyed alfalfa-producing provinces, with a prevalence of 64%. The plant virus AEV-1 is widely distributed in the country, and its transmission vector has been unknown until now. Here we show that the black aphid Aphis craccivora can transmit AEV-1. The CP sequence identity among 16 AEV-1 isolates from Argentina was from 98 to 100% and from 98.9 to 100% at nucleotide and amino acid levels, respectively, indicating a low level of sequence variation among these isolates. The phylogenetic analysis based on the complete nucleotide sequence of the CP gene indicated that AEV-1 isolates are closely related and clustered in a monophyletic group. These results suggest that AEV-1 has spread very recently in Argentina. In the present study, we report the geographical distribution of AEV-1 in the main alfalfa-growing areas of Argentina and, for the first time, identify an insect vector and describe the CP gene diversity of an enamovirus worldwide.
Instituto de Patología Vegetal
Fil: Trucco, Verónica Milagros. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal. Unidad de Fitopatología y Modernización Agrícola -UFyMA- CONICET;Argentina
Fil: Cabrera Mederos, Dariel. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal. Unidad de Fitopatología y Modernización Agrícola -UFyMA- CONICET;Argentina
Fil: Lenardon, Sergio Luis. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina. Universidad Nacional de Río Cuarto. Facultad de Agronomía y Veterinaria; Argentina
Fil: Giolitti, Fabián. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal. Unidad de Fitopatología y Modernización Agrícola -UFyMA- CONICET;Argentina
Fuente
Virus Genes (2020)
Materia
Medicago sativa
Virus de las Plantas
Enamovirus
Distribución Geográfica
Genética
Vectores
Argentina
Plant Viruses
Geographical Distribution
Genetics
Vectors
Alfalfa
Lucerne
Nivel de accesibilidad
acceso restringido
Condiciones de uso
Repositorio
INTA Digital (INTA)
Institución
Instituto Nacional de Tecnología Agropecuaria
OAI Identificador
oai:localhost:20.500.12123/7633
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spelling Geographical distribution, genetic studies and vector transmission of alfalfa enamovirus-1 infecting alfalfa crop in ArgentinaTrucco, Veronica MilagrosCabrera Mederos, DarielLenardon, Sergio LuisGiolitti, FabianMedicago sativaVirus de las PlantasEnamovirusDistribución GeográficaGenéticaVectoresArgentinaPlant VirusesGeographical DistributionGeneticsVectorsAlfalfaLucerneAlfalfa (Medicago sativa L.) growing areas of Argentina were surveyed between 2010 and 2018 to determine the geographical distribution and analyse the genetic diversity among alfalfa enamovirus-1 (AEV-1) isolates. The virus was detected in all 17 surveyed alfalfa-producing provinces, with a prevalence of 64%. The plant virus AEV-1 is widely distributed in the country, and its transmission vector has been unknown until now. Here we show that the black aphid Aphis craccivora can transmit AEV-1. The CP sequence identity among 16 AEV-1 isolates from Argentina was from 98 to 100% and from 98.9 to 100% at nucleotide and amino acid levels, respectively, indicating a low level of sequence variation among these isolates. The phylogenetic analysis based on the complete nucleotide sequence of the CP gene indicated that AEV-1 isolates are closely related and clustered in a monophyletic group. These results suggest that AEV-1 has spread very recently in Argentina. In the present study, we report the geographical distribution of AEV-1 in the main alfalfa-growing areas of Argentina and, for the first time, identify an insect vector and describe the CP gene diversity of an enamovirus worldwide.Instituto de Patología VegetalFil: Trucco, Verónica Milagros. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal. Unidad de Fitopatología y Modernización Agrícola -UFyMA- CONICET;ArgentinaFil: Cabrera Mederos, Dariel. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal. Unidad de Fitopatología y Modernización Agrícola -UFyMA- CONICET;ArgentinaFil: Lenardon, Sergio Luis. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina. Universidad Nacional de Río Cuarto. Facultad de Agronomía y Veterinaria; ArgentinaFil: Giolitti, Fabián. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal. Unidad de Fitopatología y Modernización Agrícola -UFyMA- CONICET;ArgentinaSpringer2020-07-29T12:20:58Z2020-07-29T12:20:58Z2020-07info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://hdl.handle.net/20.500.12123/7633https://link.springer.com/article/10.1007/s11262-020-01783-z0920-85691572-994Xhttps://doi.org/10.1007/s11262-020-01783-zVirus Genes (2020)reponame:INTA Digital (INTA)instname:Instituto Nacional de Tecnología Agropecuariaenginfo:eu-repo/semantics/restrictedAccess2025-09-29T13:44:59Zoai:localhost:20.500.12123/7633instacron:INTAInstitucionalhttp://repositorio.inta.gob.ar/Organismo científico-tecnológicoNo correspondehttp://repositorio.inta.gob.ar/oai/requesttripaldi.nicolas@inta.gob.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:l2025-09-29 13:45:00.015INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuariafalse
dc.title.none.fl_str_mv Geographical distribution, genetic studies and vector transmission of alfalfa enamovirus-1 infecting alfalfa crop in Argentina
title Geographical distribution, genetic studies and vector transmission of alfalfa enamovirus-1 infecting alfalfa crop in Argentina
spellingShingle Geographical distribution, genetic studies and vector transmission of alfalfa enamovirus-1 infecting alfalfa crop in Argentina
Trucco, Veronica Milagros
Medicago sativa
Virus de las Plantas
Enamovirus
Distribución Geográfica
Genética
Vectores
Argentina
Plant Viruses
Geographical Distribution
Genetics
Vectors
Alfalfa
Lucerne
title_short Geographical distribution, genetic studies and vector transmission of alfalfa enamovirus-1 infecting alfalfa crop in Argentina
title_full Geographical distribution, genetic studies and vector transmission of alfalfa enamovirus-1 infecting alfalfa crop in Argentina
title_fullStr Geographical distribution, genetic studies and vector transmission of alfalfa enamovirus-1 infecting alfalfa crop in Argentina
title_full_unstemmed Geographical distribution, genetic studies and vector transmission of alfalfa enamovirus-1 infecting alfalfa crop in Argentina
title_sort Geographical distribution, genetic studies and vector transmission of alfalfa enamovirus-1 infecting alfalfa crop in Argentina
dc.creator.none.fl_str_mv Trucco, Veronica Milagros
Cabrera Mederos, Dariel
Lenardon, Sergio Luis
Giolitti, Fabian
author Trucco, Veronica Milagros
author_facet Trucco, Veronica Milagros
Cabrera Mederos, Dariel
Lenardon, Sergio Luis
Giolitti, Fabian
author_role author
author2 Cabrera Mederos, Dariel
Lenardon, Sergio Luis
Giolitti, Fabian
author2_role author
author
author
dc.subject.none.fl_str_mv Medicago sativa
Virus de las Plantas
Enamovirus
Distribución Geográfica
Genética
Vectores
Argentina
Plant Viruses
Geographical Distribution
Genetics
Vectors
Alfalfa
Lucerne
topic Medicago sativa
Virus de las Plantas
Enamovirus
Distribución Geográfica
Genética
Vectores
Argentina
Plant Viruses
Geographical Distribution
Genetics
Vectors
Alfalfa
Lucerne
dc.description.none.fl_txt_mv Alfalfa (Medicago sativa L.) growing areas of Argentina were surveyed between 2010 and 2018 to determine the geographical distribution and analyse the genetic diversity among alfalfa enamovirus-1 (AEV-1) isolates. The virus was detected in all 17 surveyed alfalfa-producing provinces, with a prevalence of 64%. The plant virus AEV-1 is widely distributed in the country, and its transmission vector has been unknown until now. Here we show that the black aphid Aphis craccivora can transmit AEV-1. The CP sequence identity among 16 AEV-1 isolates from Argentina was from 98 to 100% and from 98.9 to 100% at nucleotide and amino acid levels, respectively, indicating a low level of sequence variation among these isolates. The phylogenetic analysis based on the complete nucleotide sequence of the CP gene indicated that AEV-1 isolates are closely related and clustered in a monophyletic group. These results suggest that AEV-1 has spread very recently in Argentina. In the present study, we report the geographical distribution of AEV-1 in the main alfalfa-growing areas of Argentina and, for the first time, identify an insect vector and describe the CP gene diversity of an enamovirus worldwide.
Instituto de Patología Vegetal
Fil: Trucco, Verónica Milagros. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal. Unidad de Fitopatología y Modernización Agrícola -UFyMA- CONICET;Argentina
Fil: Cabrera Mederos, Dariel. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal. Unidad de Fitopatología y Modernización Agrícola -UFyMA- CONICET;Argentina
Fil: Lenardon, Sergio Luis. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina. Universidad Nacional de Río Cuarto. Facultad de Agronomía y Veterinaria; Argentina
Fil: Giolitti, Fabián. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal. Unidad de Fitopatología y Modernización Agrícola -UFyMA- CONICET;Argentina
description Alfalfa (Medicago sativa L.) growing areas of Argentina were surveyed between 2010 and 2018 to determine the geographical distribution and analyse the genetic diversity among alfalfa enamovirus-1 (AEV-1) isolates. The virus was detected in all 17 surveyed alfalfa-producing provinces, with a prevalence of 64%. The plant virus AEV-1 is widely distributed in the country, and its transmission vector has been unknown until now. Here we show that the black aphid Aphis craccivora can transmit AEV-1. The CP sequence identity among 16 AEV-1 isolates from Argentina was from 98 to 100% and from 98.9 to 100% at nucleotide and amino acid levels, respectively, indicating a low level of sequence variation among these isolates. The phylogenetic analysis based on the complete nucleotide sequence of the CP gene indicated that AEV-1 isolates are closely related and clustered in a monophyletic group. These results suggest that AEV-1 has spread very recently in Argentina. In the present study, we report the geographical distribution of AEV-1 in the main alfalfa-growing areas of Argentina and, for the first time, identify an insect vector and describe the CP gene diversity of an enamovirus worldwide.
publishDate 2020
dc.date.none.fl_str_mv 2020-07-29T12:20:58Z
2020-07-29T12:20:58Z
2020-07
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/20.500.12123/7633
https://link.springer.com/article/10.1007/s11262-020-01783-z
0920-8569
1572-994X
https://doi.org/10.1007/s11262-020-01783-z
url http://hdl.handle.net/20.500.12123/7633
https://link.springer.com/article/10.1007/s11262-020-01783-z
https://doi.org/10.1007/s11262-020-01783-z
identifier_str_mv 0920-8569
1572-994X
dc.language.none.fl_str_mv eng
language eng
dc.rights.none.fl_str_mv info:eu-repo/semantics/restrictedAccess
eu_rights_str_mv restrictedAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Springer
publisher.none.fl_str_mv Springer
dc.source.none.fl_str_mv Virus Genes (2020)
reponame:INTA Digital (INTA)
instname:Instituto Nacional de Tecnología Agropecuaria
reponame_str INTA Digital (INTA)
collection INTA Digital (INTA)
instname_str Instituto Nacional de Tecnología Agropecuaria
repository.name.fl_str_mv INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuaria
repository.mail.fl_str_mv tripaldi.nicolas@inta.gob.ar
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score 12.559606

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