Complete genome classification system of rotavirus alphagastroenteritidis : An updated analysis

Autores
Díaz Alarcón, Ricardo Gabriel; Salvatierra, Karina; Gómez Quintero, Emiliano; Liotta, Domingo Javier; Parreño, Gladys Viviana; Miño, Samuel
Año de publicación
2025
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Rotavirus alphagastroenteritidis is the major causative agent of acute gastroenteritis in both children under the age of 5 and young mammals and birds globally. RVAs are non-enveloped viruses with a genome comprising 11 double-stranded RNA segments. In 2008, the Rotavirus Classification Working Group pioneered a comprehensive and complete RVA genome classification system, establishing a specific threshold, which measures the genetic distances between homologous genes. The aim of this study was to perform an updated systematic analysis of the genetic variability across all RVA genes. Our investigation involved assessing the established cutoff values for each RVA genome segment and determining the need for any updates. To achieve this objective, multiple sequence alignments were constructed for all 11 genes and one for each genotype with discrepancies. Also, pairwise distances along with their cutoff values were evaluated. The analyses provided insights into the current relevance of cutoff values, which remain applicable for the majority of genotypes. In conclusion, this study fortifies the current classification system by highlighting its robustness and accurate genotyping of Rotavirus alphagastroenteritidis.
Instituto de Virología
Fil: Díaz Alarcón, Ricardo G. Universidad Nacional de Misiones. Facultad de Ciencias Exactas, Químicas y Naturales. Laboratorio de Biología Molecular Aplicada; Argentina
Fil: Díaz Alarcón, Ricardo G. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Salvatierra, Karina. Universidad Nacional de Misiones. Laboratorio “MADAR”; Argentina
Fil: Gómez Quintero, Emiliano. Universidad Nacional de Misiones. Facultad de Ciencias Exactas, Químicas y Naturales. Laboratorio de Biología Molecular Aplicada; Argentina
Fil: Liotta, Domingo Javier. Universidad Nacional de Misiones. Facultad de Ciencias Exactas, Químicas y Naturales. Laboratorio de Biología Molecular Aplicada; Argentina
Fil: Liotta, Domingo Javier. Administración Nacional de Laboratorios e Institutos de Salud (ANLIS) “Dr. Carlos Malbrán”. Instituto Nacional de Medicina Tropical; Argentina
Fil: Parreño, Gladys Viviana. Instituto Nacional de Tecnología Agropecuaria (INTA). INCUINTA. Instituto de Virologia e Innovaciones Tecnologicas (IVIT); Argentina
Fil: Miño, Samuel. Universidad Nacional de Misiones. Facultad de Ciencias Exactas, Químicas y Naturales. Laboratorio de Biología Molecular Aplicada; Argentina
Fil: Miño, Samuel. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Cerro Azul; Argentina
Fuente
Viruses 17 (2) : 211 (February 2025)
Materia
Phylogeny
Genomes
Rotavirus
Gastroenteritis
Genetic Variation
Filogenia
Genoma
Variación Genética
Rotavirus alphagastroenteritidis
Nivel de accesibilidad
acceso abierto
Condiciones de uso
http://creativecommons.org/licenses/by-nc-sa/4.0/
Repositorio
INTA Digital (INTA)
Institución
Instituto Nacional de Tecnología Agropecuaria
OAI Identificador
oai:localhost:20.500.12123/21512
Acceder
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oai_identifier_str oai:localhost:20.500.12123/21512
network_acronym_str INTADig
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network_name_str INTA Digital (INTA)
spelling Complete genome classification system of rotavirus alphagastroenteritidis : An updated analysisDíaz Alarcón, Ricardo GabrielSalvatierra, KarinaGómez Quintero, EmilianoLiotta, Domingo JavierParreño, Gladys VivianaMiño, SamuelPhylogenyGenomesRotavirusGastroenteritisGenetic VariationFilogeniaGenomaVariación GenéticaRotavirus alphagastroenteritidisRotavirus alphagastroenteritidis is the major causative agent of acute gastroenteritis in both children under the age of 5 and young mammals and birds globally. RVAs are non-enveloped viruses with a genome comprising 11 double-stranded RNA segments. In 2008, the Rotavirus Classification Working Group pioneered a comprehensive and complete RVA genome classification system, establishing a specific threshold, which measures the genetic distances between homologous genes. The aim of this study was to perform an updated systematic analysis of the genetic variability across all RVA genes. Our investigation involved assessing the established cutoff values for each RVA genome segment and determining the need for any updates. To achieve this objective, multiple sequence alignments were constructed for all 11 genes and one for each genotype with discrepancies. Also, pairwise distances along with their cutoff values were evaluated. The analyses provided insights into the current relevance of cutoff values, which remain applicable for the majority of genotypes. In conclusion, this study fortifies the current classification system by highlighting its robustness and accurate genotyping of Rotavirus alphagastroenteritidis.Instituto de VirologíaFil: Díaz Alarcón, Ricardo G. Universidad Nacional de Misiones. Facultad de Ciencias Exactas, Químicas y Naturales. Laboratorio de Biología Molecular Aplicada; ArgentinaFil: Díaz Alarcón, Ricardo G. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Salvatierra, Karina. Universidad Nacional de Misiones. Laboratorio “MADAR”; ArgentinaFil: Gómez Quintero, Emiliano. Universidad Nacional de Misiones. Facultad de Ciencias Exactas, Químicas y Naturales. Laboratorio de Biología Molecular Aplicada; ArgentinaFil: Liotta, Domingo Javier. Universidad Nacional de Misiones. Facultad de Ciencias Exactas, Químicas y Naturales. Laboratorio de Biología Molecular Aplicada; ArgentinaFil: Liotta, Domingo Javier. Administración Nacional de Laboratorios e Institutos de Salud (ANLIS) “Dr. Carlos Malbrán”. Instituto Nacional de Medicina Tropical; ArgentinaFil: Parreño, Gladys Viviana. Instituto Nacional de Tecnología Agropecuaria (INTA). INCUINTA. Instituto de Virologia e Innovaciones Tecnologicas (IVIT); ArgentinaFil: Miño, Samuel. Universidad Nacional de Misiones. Facultad de Ciencias Exactas, Químicas y Naturales. Laboratorio de Biología Molecular Aplicada; ArgentinaFil: Miño, Samuel. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Cerro Azul; ArgentinaMDPI2025-02-28T13:05:37Z2025-02-28T13:05:37Z2025-02info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://hdl.handle.net/20.500.12123/21512https://www.mdpi.com/1999-4915/17/2/2111999-4915https://doi.org/10.3390/v17020211Viruses 17 (2) : 211 (February 2025)reponame:INTA Digital (INTA)instname:Instituto Nacional de Tecnología Agropecuariaenginfo:eu-repograntAgreement/INTA/2023-PE-L01-I044, Enfermedades subtropicales con impacto sobre la producción, salud pública y sustentabilidad en la ganadería del norte de Argentinainfo:eu-repograntAgreement/INTA/2019-RIST-E5-I111-001, Laboratorios de Diagnóstico Veterinarioinfo:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by-nc-sa/4.0/Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)2025-09-11T10:25:37Zoai:localhost:20.500.12123/21512instacron:INTAInstitucionalhttp://repositorio.inta.gob.ar/Organismo científico-tecnológicoNo correspondehttp://repositorio.inta.gob.ar/oai/requesttripaldi.nicolas@inta.gob.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:l2025-09-11 10:25:38.167INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuariafalse
dc.title.none.fl_str_mv Complete genome classification system of rotavirus alphagastroenteritidis : An updated analysis
title Complete genome classification system of rotavirus alphagastroenteritidis : An updated analysis
spellingShingle Complete genome classification system of rotavirus alphagastroenteritidis : An updated analysis
Díaz Alarcón, Ricardo Gabriel
Phylogeny
Genomes
Rotavirus
Gastroenteritis
Genetic Variation
Filogenia
Genoma
Variación Genética
Rotavirus alphagastroenteritidis
title_short Complete genome classification system of rotavirus alphagastroenteritidis : An updated analysis
title_full Complete genome classification system of rotavirus alphagastroenteritidis : An updated analysis
title_fullStr Complete genome classification system of rotavirus alphagastroenteritidis : An updated analysis
title_full_unstemmed Complete genome classification system of rotavirus alphagastroenteritidis : An updated analysis
title_sort Complete genome classification system of rotavirus alphagastroenteritidis : An updated analysis
dc.creator.none.fl_str_mv Díaz Alarcón, Ricardo Gabriel
Salvatierra, Karina
Gómez Quintero, Emiliano
Liotta, Domingo Javier
Parreño, Gladys Viviana
Miño, Samuel
author Díaz Alarcón, Ricardo Gabriel
author_facet Díaz Alarcón, Ricardo Gabriel
Salvatierra, Karina
Gómez Quintero, Emiliano
Liotta, Domingo Javier
Parreño, Gladys Viviana
Miño, Samuel
author_role author
author2 Salvatierra, Karina
Gómez Quintero, Emiliano
Liotta, Domingo Javier
Parreño, Gladys Viviana
Miño, Samuel
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv Phylogeny
Genomes
Rotavirus
Gastroenteritis
Genetic Variation
Filogenia
Genoma
Variación Genética
Rotavirus alphagastroenteritidis
topic Phylogeny
Genomes
Rotavirus
Gastroenteritis
Genetic Variation
Filogenia
Genoma
Variación Genética
Rotavirus alphagastroenteritidis
dc.description.none.fl_txt_mv Rotavirus alphagastroenteritidis is the major causative agent of acute gastroenteritis in both children under the age of 5 and young mammals and birds globally. RVAs are non-enveloped viruses with a genome comprising 11 double-stranded RNA segments. In 2008, the Rotavirus Classification Working Group pioneered a comprehensive and complete RVA genome classification system, establishing a specific threshold, which measures the genetic distances between homologous genes. The aim of this study was to perform an updated systematic analysis of the genetic variability across all RVA genes. Our investigation involved assessing the established cutoff values for each RVA genome segment and determining the need for any updates. To achieve this objective, multiple sequence alignments were constructed for all 11 genes and one for each genotype with discrepancies. Also, pairwise distances along with their cutoff values were evaluated. The analyses provided insights into the current relevance of cutoff values, which remain applicable for the majority of genotypes. In conclusion, this study fortifies the current classification system by highlighting its robustness and accurate genotyping of Rotavirus alphagastroenteritidis.
Instituto de Virología
Fil: Díaz Alarcón, Ricardo G. Universidad Nacional de Misiones. Facultad de Ciencias Exactas, Químicas y Naturales. Laboratorio de Biología Molecular Aplicada; Argentina
Fil: Díaz Alarcón, Ricardo G. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Salvatierra, Karina. Universidad Nacional de Misiones. Laboratorio “MADAR”; Argentina
Fil: Gómez Quintero, Emiliano. Universidad Nacional de Misiones. Facultad de Ciencias Exactas, Químicas y Naturales. Laboratorio de Biología Molecular Aplicada; Argentina
Fil: Liotta, Domingo Javier. Universidad Nacional de Misiones. Facultad de Ciencias Exactas, Químicas y Naturales. Laboratorio de Biología Molecular Aplicada; Argentina
Fil: Liotta, Domingo Javier. Administración Nacional de Laboratorios e Institutos de Salud (ANLIS) “Dr. Carlos Malbrán”. Instituto Nacional de Medicina Tropical; Argentina
Fil: Parreño, Gladys Viviana. Instituto Nacional de Tecnología Agropecuaria (INTA). INCUINTA. Instituto de Virologia e Innovaciones Tecnologicas (IVIT); Argentina
Fil: Miño, Samuel. Universidad Nacional de Misiones. Facultad de Ciencias Exactas, Químicas y Naturales. Laboratorio de Biología Molecular Aplicada; Argentina
Fil: Miño, Samuel. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Cerro Azul; Argentina
description Rotavirus alphagastroenteritidis is the major causative agent of acute gastroenteritis in both children under the age of 5 and young mammals and birds globally. RVAs are non-enveloped viruses with a genome comprising 11 double-stranded RNA segments. In 2008, the Rotavirus Classification Working Group pioneered a comprehensive and complete RVA genome classification system, establishing a specific threshold, which measures the genetic distances between homologous genes. The aim of this study was to perform an updated systematic analysis of the genetic variability across all RVA genes. Our investigation involved assessing the established cutoff values for each RVA genome segment and determining the need for any updates. To achieve this objective, multiple sequence alignments were constructed for all 11 genes and one for each genotype with discrepancies. Also, pairwise distances along with their cutoff values were evaluated. The analyses provided insights into the current relevance of cutoff values, which remain applicable for the majority of genotypes. In conclusion, this study fortifies the current classification system by highlighting its robustness and accurate genotyping of Rotavirus alphagastroenteritidis.
publishDate 2025
dc.date.none.fl_str_mv 2025-02-28T13:05:37Z
2025-02-28T13:05:37Z
2025-02
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/20.500.12123/21512
https://www.mdpi.com/1999-4915/17/2/211
1999-4915
https://doi.org/10.3390/v17020211
url http://hdl.handle.net/20.500.12123/21512
https://www.mdpi.com/1999-4915/17/2/211
https://doi.org/10.3390/v17020211
identifier_str_mv 1999-4915
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repograntAgreement/INTA/2023-PE-L01-I044, Enfermedades subtropicales con impacto sobre la producción, salud pública y sustentabilidad en la ganadería del norte de Argentina
info:eu-repograntAgreement/INTA/2019-RIST-E5-I111-001, Laboratorios de Diagnóstico Veterinario
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
eu_rights_str_mv openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv MDPI
publisher.none.fl_str_mv MDPI
dc.source.none.fl_str_mv Viruses 17 (2) : 211 (February 2025)
reponame:INTA Digital (INTA)
instname:Instituto Nacional de Tecnología Agropecuaria
reponame_str INTA Digital (INTA)
collection INTA Digital (INTA)
instname_str Instituto Nacional de Tecnología Agropecuaria
repository.name.fl_str_mv INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuaria
repository.mail.fl_str_mv tripaldi.nicolas@inta.gob.ar
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