Reduction in acute bee paralysis virus Infection and mortality in honey bees (Apis mellifera) by RNA interference technology

Autores
Ferrufino, Cecilia Gabriela; Scannapieco, Alejandra Carla; Russo, Romina Maria; Gonzalez, Fernanda Noemi; Salvador, Ricardo; Dus Santos, Maria Jose
Año de publicación
2025
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
In Argentina, various studies have reported the detection of multiple viruses in honey-producing and queen-rearing apiaries, with Aparavirus apisacutum, the causal agent of acute bee paralysis (ABP), demonstrating a particularly high prevalence. The potential of RNA interference (RNAi) as a strategy to control honey bee viruses has been explored, with initial findings indicating that RNAi could aid in mitigating the economic losses associated with viral infections. This study aimed to evaluate the effect of RNAi technology mediated by double-stranded RNA (dsRNA) on the dynamics of ABPV infection in adult honey bees. Fragments of the ABPV replicase and VP1 genes were used as templates for dsRNA synthesis via in vitro transcription. A gene silencing experiment was conducted through oral administration using five treatments: control, specific dsRNA + Virus, Virus alone, specific dsRNA alone, and non-specific dsRNA + virus. Bee survival was recorded over 10 days for all treatments, and samples were subsequently processed for viral quantification using quantitative real-time PCR. The oral administration of specific dsRNA reduced the viral replication curve, decreased the average viral loads and increased bee survival. This is the first report demonstrating the reduction in ABPV infection in adult honey bees through post-transcriptional gene silencing achieved via oral administration of dsRNA.
Instituto de Virología
Fil: Ferrufino, Cecilia Gabriela. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología e Innovaciones Tecnológicas; Argentina
Fil: Ferrufino, Cecilia Gabriela. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Scannapieco, Alejandra Carla. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética; Argentina
Fil: Scannapieco, Alejandra Carla. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina
Fil: Scannapieco, Alejandra Carla. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Russo, Romina Maria. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética; Argentina
Fil: Russo, Romina Maria. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina
Fil: Russo, Romina Maria. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Gonzalez, Fernanda Noemi. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología e Innovaciones Tecnológicas; Argentina
Fil: Gonzalez, Fernanda Noemi. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Salvador, Ricardo. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Microbiología y Zoología Agrícola; Argentina
Fil: Dus Santos, María José. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología e Innovaciones Tecnológicas; Argentina
Fil: Dus Santos, María José. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fuente
Insects 16 (5) : 453 (May 2025)
Materia
Acute Bee Paralysis Virus
dsRNA Viruses
Honey Bees
Survival
Apis mellifera
Gene Silencing
Virus de Parálisis Aguda de la Abeja
Virus ARN Bicatenario
Abeja Melífera
Supervivencia
Silenciamiento Genético
Argentina
Aparavirus apisacutum
Nivel de accesibilidad
acceso abierto
Condiciones de uso
http://creativecommons.org/licenses/by-nc-sa/4.0/
Repositorio
INTA Digital (INTA)
Institución
Instituto Nacional de Tecnología Agropecuaria
OAI Identificador
oai:localhost:20.500.12123/22390
Acceder
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oai_identifier_str oai:localhost:20.500.12123/22390
network_acronym_str INTADig
repository_id_str l
network_name_str INTA Digital (INTA)
spelling Reduction in acute bee paralysis virus Infection and mortality in honey bees (Apis mellifera) by RNA interference technologyFerrufino, Cecilia GabrielaScannapieco, Alejandra CarlaRusso, Romina MariaGonzalez, Fernanda NoemiSalvador, RicardoDus Santos, Maria JoseAcute Bee Paralysis VirusdsRNA VirusesHoney BeesSurvivalApis melliferaGene SilencingVirus de Parálisis Aguda de la AbejaVirus ARN BicatenarioAbeja MelíferaSupervivenciaSilenciamiento GenéticoArgentinaAparavirus apisacutumIn Argentina, various studies have reported the detection of multiple viruses in honey-producing and queen-rearing apiaries, with Aparavirus apisacutum, the causal agent of acute bee paralysis (ABP), demonstrating a particularly high prevalence. The potential of RNA interference (RNAi) as a strategy to control honey bee viruses has been explored, with initial findings indicating that RNAi could aid in mitigating the economic losses associated with viral infections. This study aimed to evaluate the effect of RNAi technology mediated by double-stranded RNA (dsRNA) on the dynamics of ABPV infection in adult honey bees. Fragments of the ABPV replicase and VP1 genes were used as templates for dsRNA synthesis via in vitro transcription. A gene silencing experiment was conducted through oral administration using five treatments: control, specific dsRNA + Virus, Virus alone, specific dsRNA alone, and non-specific dsRNA + virus. Bee survival was recorded over 10 days for all treatments, and samples were subsequently processed for viral quantification using quantitative real-time PCR. The oral administration of specific dsRNA reduced the viral replication curve, decreased the average viral loads and increased bee survival. This is the first report demonstrating the reduction in ABPV infection in adult honey bees through post-transcriptional gene silencing achieved via oral administration of dsRNA.Instituto de VirologíaFil: Ferrufino, Cecilia Gabriela. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología e Innovaciones Tecnológicas; ArgentinaFil: Ferrufino, Cecilia Gabriela. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Scannapieco, Alejandra Carla. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética; ArgentinaFil: Scannapieco, Alejandra Carla. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; ArgentinaFil: Scannapieco, Alejandra Carla. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Russo, Romina Maria. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética; ArgentinaFil: Russo, Romina Maria. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; ArgentinaFil: Russo, Romina Maria. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Gonzalez, Fernanda Noemi. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología e Innovaciones Tecnológicas; ArgentinaFil: Gonzalez, Fernanda Noemi. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Salvador, Ricardo. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Microbiología y Zoología Agrícola; ArgentinaFil: Dus Santos, María José. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología e Innovaciones Tecnológicas; ArgentinaFil: Dus Santos, María José. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaMDPI2025-05-22T14:40:49Z2025-05-22T14:40:49Z2025-05info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://hdl.handle.net/20.500.12123/22390https://www.mdpi.com/2075-4450/16/5/4532075-4450https://doi.org/10.3390/insects16050453Insects 16 (5) : 453 (May 2025)reponame:INTA Digital (INTA)instname:Instituto Nacional de Tecnología Agropecuariaenginfo:eu-repograntAgreement/INTA/2023-PE-L01-I069, Aportes al desarrollo sostenible de la apicultura argentinainfo:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by-nc-sa/4.0/Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)2025-09-29T13:47:19Zoai:localhost:20.500.12123/22390instacron:INTAInstitucionalhttp://repositorio.inta.gob.ar/Organismo científico-tecnológicoNo correspondehttp://repositorio.inta.gob.ar/oai/requesttripaldi.nicolas@inta.gob.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:l2025-09-29 13:47:19.512INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuariafalse
dc.title.none.fl_str_mv Reduction in acute bee paralysis virus Infection and mortality in honey bees (Apis mellifera) by RNA interference technology
title Reduction in acute bee paralysis virus Infection and mortality in honey bees (Apis mellifera) by RNA interference technology
spellingShingle Reduction in acute bee paralysis virus Infection and mortality in honey bees (Apis mellifera) by RNA interference technology
Ferrufino, Cecilia Gabriela
Acute Bee Paralysis Virus
dsRNA Viruses
Honey Bees
Survival
Apis mellifera
Gene Silencing
Virus de Parálisis Aguda de la Abeja
Virus ARN Bicatenario
Abeja Melífera
Supervivencia
Silenciamiento Genético
Argentina
Aparavirus apisacutum
title_short Reduction in acute bee paralysis virus Infection and mortality in honey bees (Apis mellifera) by RNA interference technology
title_full Reduction in acute bee paralysis virus Infection and mortality in honey bees (Apis mellifera) by RNA interference technology
title_fullStr Reduction in acute bee paralysis virus Infection and mortality in honey bees (Apis mellifera) by RNA interference technology
title_full_unstemmed Reduction in acute bee paralysis virus Infection and mortality in honey bees (Apis mellifera) by RNA interference technology
title_sort Reduction in acute bee paralysis virus Infection and mortality in honey bees (Apis mellifera) by RNA interference technology
dc.creator.none.fl_str_mv Ferrufino, Cecilia Gabriela
Scannapieco, Alejandra Carla
Russo, Romina Maria
Gonzalez, Fernanda Noemi
Salvador, Ricardo
Dus Santos, Maria Jose
author Ferrufino, Cecilia Gabriela
author_facet Ferrufino, Cecilia Gabriela
Scannapieco, Alejandra Carla
Russo, Romina Maria
Gonzalez, Fernanda Noemi
Salvador, Ricardo
Dus Santos, Maria Jose
author_role author
author2 Scannapieco, Alejandra Carla
Russo, Romina Maria
Gonzalez, Fernanda Noemi
Salvador, Ricardo
Dus Santos, Maria Jose
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv Acute Bee Paralysis Virus
dsRNA Viruses
Honey Bees
Survival
Apis mellifera
Gene Silencing
Virus de Parálisis Aguda de la Abeja
Virus ARN Bicatenario
Abeja Melífera
Supervivencia
Silenciamiento Genético
Argentina
Aparavirus apisacutum
topic Acute Bee Paralysis Virus
dsRNA Viruses
Honey Bees
Survival
Apis mellifera
Gene Silencing
Virus de Parálisis Aguda de la Abeja
Virus ARN Bicatenario
Abeja Melífera
Supervivencia
Silenciamiento Genético
Argentina
Aparavirus apisacutum
dc.description.none.fl_txt_mv In Argentina, various studies have reported the detection of multiple viruses in honey-producing and queen-rearing apiaries, with Aparavirus apisacutum, the causal agent of acute bee paralysis (ABP), demonstrating a particularly high prevalence. The potential of RNA interference (RNAi) as a strategy to control honey bee viruses has been explored, with initial findings indicating that RNAi could aid in mitigating the economic losses associated with viral infections. This study aimed to evaluate the effect of RNAi technology mediated by double-stranded RNA (dsRNA) on the dynamics of ABPV infection in adult honey bees. Fragments of the ABPV replicase and VP1 genes were used as templates for dsRNA synthesis via in vitro transcription. A gene silencing experiment was conducted through oral administration using five treatments: control, specific dsRNA + Virus, Virus alone, specific dsRNA alone, and non-specific dsRNA + virus. Bee survival was recorded over 10 days for all treatments, and samples were subsequently processed for viral quantification using quantitative real-time PCR. The oral administration of specific dsRNA reduced the viral replication curve, decreased the average viral loads and increased bee survival. This is the first report demonstrating the reduction in ABPV infection in adult honey bees through post-transcriptional gene silencing achieved via oral administration of dsRNA.
Instituto de Virología
Fil: Ferrufino, Cecilia Gabriela. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología e Innovaciones Tecnológicas; Argentina
Fil: Ferrufino, Cecilia Gabriela. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Scannapieco, Alejandra Carla. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética; Argentina
Fil: Scannapieco, Alejandra Carla. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina
Fil: Scannapieco, Alejandra Carla. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Russo, Romina Maria. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética; Argentina
Fil: Russo, Romina Maria. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina
Fil: Russo, Romina Maria. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Gonzalez, Fernanda Noemi. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología e Innovaciones Tecnológicas; Argentina
Fil: Gonzalez, Fernanda Noemi. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Salvador, Ricardo. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Microbiología y Zoología Agrícola; Argentina
Fil: Dus Santos, María José. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología e Innovaciones Tecnológicas; Argentina
Fil: Dus Santos, María José. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
description In Argentina, various studies have reported the detection of multiple viruses in honey-producing and queen-rearing apiaries, with Aparavirus apisacutum, the causal agent of acute bee paralysis (ABP), demonstrating a particularly high prevalence. The potential of RNA interference (RNAi) as a strategy to control honey bee viruses has been explored, with initial findings indicating that RNAi could aid in mitigating the economic losses associated with viral infections. This study aimed to evaluate the effect of RNAi technology mediated by double-stranded RNA (dsRNA) on the dynamics of ABPV infection in adult honey bees. Fragments of the ABPV replicase and VP1 genes were used as templates for dsRNA synthesis via in vitro transcription. A gene silencing experiment was conducted through oral administration using five treatments: control, specific dsRNA + Virus, Virus alone, specific dsRNA alone, and non-specific dsRNA + virus. Bee survival was recorded over 10 days for all treatments, and samples were subsequently processed for viral quantification using quantitative real-time PCR. The oral administration of specific dsRNA reduced the viral replication curve, decreased the average viral loads and increased bee survival. This is the first report demonstrating the reduction in ABPV infection in adult honey bees through post-transcriptional gene silencing achieved via oral administration of dsRNA.
publishDate 2025
dc.date.none.fl_str_mv 2025-05-22T14:40:49Z
2025-05-22T14:40:49Z
2025-05
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/20.500.12123/22390
https://www.mdpi.com/2075-4450/16/5/453
2075-4450
https://doi.org/10.3390/insects16050453
url http://hdl.handle.net/20.500.12123/22390
https://www.mdpi.com/2075-4450/16/5/453
https://doi.org/10.3390/insects16050453
identifier_str_mv 2075-4450
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repograntAgreement/INTA/2023-PE-L01-I069, Aportes al desarrollo sostenible de la apicultura argentina
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
eu_rights_str_mv openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv MDPI
publisher.none.fl_str_mv MDPI
dc.source.none.fl_str_mv Insects 16 (5) : 453 (May 2025)
reponame:INTA Digital (INTA)
instname:Instituto Nacional de Tecnología Agropecuaria
reponame_str INTA Digital (INTA)
collection INTA Digital (INTA)
instname_str Instituto Nacional de Tecnología Agropecuaria
repository.name.fl_str_mv INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuaria
repository.mail.fl_str_mv tripaldi.nicolas@inta.gob.ar
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score 12.559606

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