Bacterially expressed dsRNA induces Varroa destructor gene knockdown by honey bee-mediated oral administration

Autores
Muntaabski, Irina; Scannapieco, Alejandra Carla; Liendo, María Clara; Niz, José María; Russo, Romina Maria; Salvador, Ricardo
Año de publicación
2022
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The ectoparasite Varroa destructor causes serious losses of Apis mellifera colonies and negatively impacts the beekeeping industry around the world. New control methods have been proposed based on the RNA interference technique. Previous reports showed that parasitized honey bees fed with double-stranded RNA (dsRNA) synthesized in vitro reduce the transcription levels of target genes in Varroa mites. An efficient and inexpensive alternative to produce dsRNA is the use of bacteria capable of achieving high levels of in vivo synthesis. In the present study, dsRNA synthetized in vivo was used to induce gene silencing in V. destructor and evaluate their effect on the survival of both honey bees and the parasitic Varroa mites. The results evidenced that dsRNA fed to the bees engendered gene silencing in mites, inhibiting expression levels of target genes by 50%. Indeed, a reduction of 50% in Varroa survival was observed when bacterially expressed dsRNAs were administered to mite-parasitized bees. Worker bees that were fed with Varroa-targeted dsRNA by oral route showed no survival differences compared to control bees, fed with sucrose or dsRNA-GFP solutions. Our results demonstrated that specific dsRNA over-expressed in bacteria is capable of reducing mite survival by bee-mediated oral administration. This study provides an efficient and low-cost method for dsRNA production to control parasites and honey bee diseases.
Instituto de Genética
Fil: Muntaabski, Irina. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética; Argentina
Fil: Muntaabski, Irina. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina
Fil: Muntaabski, Irina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Scannapieco, Alejandra Carla. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética; Argentina
Fil: Scannapieco, Alejandra Carla. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina
Fil: Scannapieco, Alejandra Carla. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Liendo, María Clara. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética; Argentina
Fil: Liendo, María Clara. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular); Argentina
Fil: Liendo, María Clara. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Niz, José María. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Microbiología y Zoología Agrícola; Argentina
Fil: Russo, Romina Maria. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética; Argentina
Fil: Russo, Romina Maria. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina
Fil: Russo, Romina Maria. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Salvador, Ricardo. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Microbiología y Zoología Agrícola; Argentina
Fuente
Journal of Apicultural Research (Published online: 01 Feb 2022)
Materia
Varroa destructor
Abeja Melífera
Silenciamiento Genético
Experimentación in Vivo
Síntesis
Apis mellifera
Honey Bees
Gene Silencing
In Vivo Experimentation
dsRNA viruses
Synthesis
Nivel de accesibilidad
acceso restringido
Condiciones de uso
Repositorio
INTA Digital (INTA)
Institución
Instituto Nacional de Tecnología Agropecuaria
OAI Identificador
oai:localhost:20.500.12123/11611
Acceder
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oai_identifier_str oai:localhost:20.500.12123/11611
network_acronym_str INTADig
repository_id_str l
network_name_str INTA Digital (INTA)
spelling Bacterially expressed dsRNA induces Varroa destructor gene knockdown by honey bee-mediated oral administrationMuntaabski, IrinaScannapieco, Alejandra CarlaLiendo, María ClaraNiz, José MaríaRusso, Romina MariaSalvador, RicardoVarroa destructorAbeja MelíferaSilenciamiento GenéticoExperimentación in VivoSíntesisApis melliferaHoney BeesGene SilencingIn Vivo ExperimentationdsRNA virusesSynthesisThe ectoparasite Varroa destructor causes serious losses of Apis mellifera colonies and negatively impacts the beekeeping industry around the world. New control methods have been proposed based on the RNA interference technique. Previous reports showed that parasitized honey bees fed with double-stranded RNA (dsRNA) synthesized in vitro reduce the transcription levels of target genes in Varroa mites. An efficient and inexpensive alternative to produce dsRNA is the use of bacteria capable of achieving high levels of in vivo synthesis. In the present study, dsRNA synthetized in vivo was used to induce gene silencing in V. destructor and evaluate their effect on the survival of both honey bees and the parasitic Varroa mites. The results evidenced that dsRNA fed to the bees engendered gene silencing in mites, inhibiting expression levels of target genes by 50%. Indeed, a reduction of 50% in Varroa survival was observed when bacterially expressed dsRNAs were administered to mite-parasitized bees. Worker bees that were fed with Varroa-targeted dsRNA by oral route showed no survival differences compared to control bees, fed with sucrose or dsRNA-GFP solutions. Our results demonstrated that specific dsRNA over-expressed in bacteria is capable of reducing mite survival by bee-mediated oral administration. This study provides an efficient and low-cost method for dsRNA production to control parasites and honey bee diseases.Instituto de GenéticaFil: Muntaabski, Irina. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética; ArgentinaFil: Muntaabski, Irina. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; ArgentinaFil: Muntaabski, Irina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Scannapieco, Alejandra Carla. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética; ArgentinaFil: Scannapieco, Alejandra Carla. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; ArgentinaFil: Scannapieco, Alejandra Carla. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Liendo, María Clara. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética; ArgentinaFil: Liendo, María Clara. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular); ArgentinaFil: Liendo, María Clara. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Niz, José María. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Microbiología y Zoología Agrícola; ArgentinaFil: Russo, Romina Maria. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética; ArgentinaFil: Russo, Romina Maria. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; ArgentinaFil: Russo, Romina Maria. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Salvador, Ricardo. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Microbiología y Zoología Agrícola; ArgentinaTaylor and Francis2022-04-08T17:46:49Z2022-04-08T17:46:49Z2022-02info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://hdl.handle.net/20.500.12123/11611https://www.tandfonline.com/doi/abs/10.1080/00218839.2022.20289670021-8839https://doi.org/10.1080/00218839.2022.2028967Journal of Apicultural Research (Published online: 01 Feb 2022)reponame:INTA Digital (INTA)instname:Instituto Nacional de Tecnología Agropecuariaenginfo:eu-repograntAgreement/INTA/PNAPI-1112042/AR./Estrategias multidisciplinarias para mitigar el efecto del nuevo contexto ambiental y productivo sobre la colmena.info:eu-repograntAgreement/INTA/2019-PE-E1-I017-001/2019-PE-E1-I017-001/AR./DESARROLLO DEL SECTOR APÍCOLA ORGANIZADO, SUSTENTABLE Y COMPETITIVOinfo:eu-repo/semantics/restrictedAccess2025-09-29T13:45:31Zoai:localhost:20.500.12123/11611instacron:INTAInstitucionalhttp://repositorio.inta.gob.ar/Organismo científico-tecnológicoNo correspondehttp://repositorio.inta.gob.ar/oai/requesttripaldi.nicolas@inta.gob.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:l2025-09-29 13:45:31.893INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuariafalse
dc.title.none.fl_str_mv Bacterially expressed dsRNA induces Varroa destructor gene knockdown by honey bee-mediated oral administration
title Bacterially expressed dsRNA induces Varroa destructor gene knockdown by honey bee-mediated oral administration
spellingShingle Bacterially expressed dsRNA induces Varroa destructor gene knockdown by honey bee-mediated oral administration
Muntaabski, Irina
Varroa destructor
Abeja Melífera
Silenciamiento Genético
Experimentación in Vivo
Síntesis
Apis mellifera
Honey Bees
Gene Silencing
In Vivo Experimentation
dsRNA viruses
Synthesis
title_short Bacterially expressed dsRNA induces Varroa destructor gene knockdown by honey bee-mediated oral administration
title_full Bacterially expressed dsRNA induces Varroa destructor gene knockdown by honey bee-mediated oral administration
title_fullStr Bacterially expressed dsRNA induces Varroa destructor gene knockdown by honey bee-mediated oral administration
title_full_unstemmed Bacterially expressed dsRNA induces Varroa destructor gene knockdown by honey bee-mediated oral administration
title_sort Bacterially expressed dsRNA induces Varroa destructor gene knockdown by honey bee-mediated oral administration
dc.creator.none.fl_str_mv Muntaabski, Irina
Scannapieco, Alejandra Carla
Liendo, María Clara
Niz, José María
Russo, Romina Maria
Salvador, Ricardo
author Muntaabski, Irina
author_facet Muntaabski, Irina
Scannapieco, Alejandra Carla
Liendo, María Clara
Niz, José María
Russo, Romina Maria
Salvador, Ricardo
author_role author
author2 Scannapieco, Alejandra Carla
Liendo, María Clara
Niz, José María
Russo, Romina Maria
Salvador, Ricardo
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv Varroa destructor
Abeja Melífera
Silenciamiento Genético
Experimentación in Vivo
Síntesis
Apis mellifera
Honey Bees
Gene Silencing
In Vivo Experimentation
dsRNA viruses
Synthesis
topic Varroa destructor
Abeja Melífera
Silenciamiento Genético
Experimentación in Vivo
Síntesis
Apis mellifera
Honey Bees
Gene Silencing
In Vivo Experimentation
dsRNA viruses
Synthesis
dc.description.none.fl_txt_mv The ectoparasite Varroa destructor causes serious losses of Apis mellifera colonies and negatively impacts the beekeeping industry around the world. New control methods have been proposed based on the RNA interference technique. Previous reports showed that parasitized honey bees fed with double-stranded RNA (dsRNA) synthesized in vitro reduce the transcription levels of target genes in Varroa mites. An efficient and inexpensive alternative to produce dsRNA is the use of bacteria capable of achieving high levels of in vivo synthesis. In the present study, dsRNA synthetized in vivo was used to induce gene silencing in V. destructor and evaluate their effect on the survival of both honey bees and the parasitic Varroa mites. The results evidenced that dsRNA fed to the bees engendered gene silencing in mites, inhibiting expression levels of target genes by 50%. Indeed, a reduction of 50% in Varroa survival was observed when bacterially expressed dsRNAs were administered to mite-parasitized bees. Worker bees that were fed with Varroa-targeted dsRNA by oral route showed no survival differences compared to control bees, fed with sucrose or dsRNA-GFP solutions. Our results demonstrated that specific dsRNA over-expressed in bacteria is capable of reducing mite survival by bee-mediated oral administration. This study provides an efficient and low-cost method for dsRNA production to control parasites and honey bee diseases.
Instituto de Genética
Fil: Muntaabski, Irina. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética; Argentina
Fil: Muntaabski, Irina. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina
Fil: Muntaabski, Irina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Scannapieco, Alejandra Carla. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética; Argentina
Fil: Scannapieco, Alejandra Carla. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina
Fil: Scannapieco, Alejandra Carla. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Liendo, María Clara. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética; Argentina
Fil: Liendo, María Clara. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular); Argentina
Fil: Liendo, María Clara. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Niz, José María. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Microbiología y Zoología Agrícola; Argentina
Fil: Russo, Romina Maria. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética; Argentina
Fil: Russo, Romina Maria. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina
Fil: Russo, Romina Maria. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Salvador, Ricardo. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Microbiología y Zoología Agrícola; Argentina
description The ectoparasite Varroa destructor causes serious losses of Apis mellifera colonies and negatively impacts the beekeeping industry around the world. New control methods have been proposed based on the RNA interference technique. Previous reports showed that parasitized honey bees fed with double-stranded RNA (dsRNA) synthesized in vitro reduce the transcription levels of target genes in Varroa mites. An efficient and inexpensive alternative to produce dsRNA is the use of bacteria capable of achieving high levels of in vivo synthesis. In the present study, dsRNA synthetized in vivo was used to induce gene silencing in V. destructor and evaluate their effect on the survival of both honey bees and the parasitic Varroa mites. The results evidenced that dsRNA fed to the bees engendered gene silencing in mites, inhibiting expression levels of target genes by 50%. Indeed, a reduction of 50% in Varroa survival was observed when bacterially expressed dsRNAs were administered to mite-parasitized bees. Worker bees that were fed with Varroa-targeted dsRNA by oral route showed no survival differences compared to control bees, fed with sucrose or dsRNA-GFP solutions. Our results demonstrated that specific dsRNA over-expressed in bacteria is capable of reducing mite survival by bee-mediated oral administration. This study provides an efficient and low-cost method for dsRNA production to control parasites and honey bee diseases.
publishDate 2022
dc.date.none.fl_str_mv 2022-04-08T17:46:49Z
2022-04-08T17:46:49Z
2022-02
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/20.500.12123/11611
https://www.tandfonline.com/doi/abs/10.1080/00218839.2022.2028967
0021-8839
https://doi.org/10.1080/00218839.2022.2028967
url http://hdl.handle.net/20.500.12123/11611
https://www.tandfonline.com/doi/abs/10.1080/00218839.2022.2028967
https://doi.org/10.1080/00218839.2022.2028967
identifier_str_mv 0021-8839
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repograntAgreement/INTA/PNAPI-1112042/AR./Estrategias multidisciplinarias para mitigar el efecto del nuevo contexto ambiental y productivo sobre la colmena.
info:eu-repograntAgreement/INTA/2019-PE-E1-I017-001/2019-PE-E1-I017-001/AR./DESARROLLO DEL SECTOR APÍCOLA ORGANIZADO, SUSTENTABLE Y COMPETITIVO
dc.rights.none.fl_str_mv info:eu-repo/semantics/restrictedAccess
eu_rights_str_mv restrictedAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Taylor and Francis
publisher.none.fl_str_mv Taylor and Francis
dc.source.none.fl_str_mv Journal of Apicultural Research (Published online: 01 Feb 2022)
reponame:INTA Digital (INTA)
instname:Instituto Nacional de Tecnología Agropecuaria
reponame_str INTA Digital (INTA)
collection INTA Digital (INTA)
instname_str Instituto Nacional de Tecnología Agropecuaria
repository.name.fl_str_mv INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuaria
repository.mail.fl_str_mv tripaldi.nicolas@inta.gob.ar
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