Can soil properties and agricultural land use affect arbuscular mycorrhizal fungal communities indigenous from the Argentinean Pampas soils?

Autores
Thougnon Islas, Andrea Julieta; Hernandez Guijarro, Keren; Eyherabide, Mercedes; Sainz Rozas, Hernan Rene; Echeverria, Hernan Eduardo; Covacevich, Fernanda
Año de publicación
2016
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
In order to determine if intensive agricultural land use and/or edaphic characteristics modify the community of the arbuscular mycorrhizal fungi (AMF) indigenous of soils from the Argentinean Pampa; mycorrhizal colonization, sporulation, glomalin (GRSP) and genetic diversity were assessed. Soils were collected from seven localities from Buenos Aires Province (Argentina) under contrasting land use intensity (at each site both pristine and agricultural soils). The capacity for root colonization, sporulation and glomalin content of trap plants and growth substrate were considered as measure of AMF activity. Analysis of diversity was conducted using PCR-single strand conformation polymorphism (SSCP). Profiles were generated by using primers to amplify the 28S rDNA of AMF from rDNA extracted directly from the substrate of trap plant or AMF spore. Five bands of the SSCP gel were sequenced and showed similarity with the genus Glomus. Analysis of colonization and GRSP content, were not clearly related to the land use intensity but sporulation capacity was, in general, lower in agricultural soils than in pristine ones. Soil phosphorus, ferrum and zinc contents, were negatively related to the root colonization and sporulation capacities. High genetic diversity was found both from soil and spore samples. Although similar diversity was found under agricultural and pristine soils, in most sites SSCP-diversity was separately clustered by land use. Still remains clarify if soil characteristics, resulting from agricultural management; exert selection pressure on the AMF and whether they could be vectors of interest to select potential plant growth promoting microorganisms.
EEA Balcarce
Fil: Thougnon Islas, Andrea Julieta. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce. Unidad Integrada. Universidad Nacional de Mar del Plata. Facultad de Ciencias Agrarias; Argentina
Fil: Hernández Guijarro, Keren. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce. Unidad Integrada. Universidad Nacional de Mar del Plata. Facultad de Ciencias Agrarias; Argentina
Fil: Eyherabide, Mercedes. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce. Unidad Integrada. Universidad Nacional de Mar del Plata. Facultad de Ciencias Agrarias; Argentina.
Fil: Sainz Rozas, Hernan Rene. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce. Unidad Integrada. Universidad Nacional de Mar del Plata. Facultad de Ciencias Agrarias; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Echeverria, Hernan Eduardo. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce. Unidad Integrada. Universidad Nacional de Mar del Plata. Facultad de Ciencias Agrarias; Argentina
Fil: Covacevich, Fernanda. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce. Unidad Integrada. Universidad Nacional de Mar del Plata. Facultad de Ciencias Agrarias; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Mar del Plata. Instituto de Investigaciones en Biodiversidad y Biotecnología; Argentina. Fundación para Investigaciones Biológicas Aplicadas; Argentina
Fuente
Applied Soil Ecology 101 : 47-56 (May 2016)
Materia
Suelo
Propiedades Físico - Químicas Suelo
Utilización de la Tierra
Micorrizas Arbusculares
Hongos
Soil
Soil Chemicophysical Properties
Land Use
Arbuscular Mycorrhiza
Fungi
Región Pampeana
Nivel de accesibilidad
acceso restringido
Condiciones de uso
Repositorio
INTA Digital (INTA)
Institución
Instituto Nacional de Tecnología Agropecuaria
OAI Identificador
oai:localhost:20.500.12123/5288
Acceder
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oai_identifier_str oai:localhost:20.500.12123/5288
network_acronym_str INTADig
repository_id_str l
network_name_str INTA Digital (INTA)
spelling Can soil properties and agricultural land use affect arbuscular mycorrhizal fungal communities indigenous from the Argentinean Pampas soils?Thougnon Islas, Andrea JulietaHernandez Guijarro, KerenEyherabide, MercedesSainz Rozas, Hernan ReneEcheverria, Hernan EduardoCovacevich, FernandaSueloPropiedades Físico - Químicas SueloUtilización de la TierraMicorrizas ArbuscularesHongosSoilSoil Chemicophysical PropertiesLand UseArbuscular MycorrhizaFungiRegión PampeanaIn order to determine if intensive agricultural land use and/or edaphic characteristics modify the community of the arbuscular mycorrhizal fungi (AMF) indigenous of soils from the Argentinean Pampa; mycorrhizal colonization, sporulation, glomalin (GRSP) and genetic diversity were assessed. Soils were collected from seven localities from Buenos Aires Province (Argentina) under contrasting land use intensity (at each site both pristine and agricultural soils). The capacity for root colonization, sporulation and glomalin content of trap plants and growth substrate were considered as measure of AMF activity. Analysis of diversity was conducted using PCR-single strand conformation polymorphism (SSCP). Profiles were generated by using primers to amplify the 28S rDNA of AMF from rDNA extracted directly from the substrate of trap plant or AMF spore. Five bands of the SSCP gel were sequenced and showed similarity with the genus Glomus. Analysis of colonization and GRSP content, were not clearly related to the land use intensity but sporulation capacity was, in general, lower in agricultural soils than in pristine ones. Soil phosphorus, ferrum and zinc contents, were negatively related to the root colonization and sporulation capacities. High genetic diversity was found both from soil and spore samples. Although similar diversity was found under agricultural and pristine soils, in most sites SSCP-diversity was separately clustered by land use. Still remains clarify if soil characteristics, resulting from agricultural management; exert selection pressure on the AMF and whether they could be vectors of interest to select potential plant growth promoting microorganisms.EEA BalcarceFil: Thougnon Islas, Andrea Julieta. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce. Unidad Integrada. Universidad Nacional de Mar del Plata. Facultad de Ciencias Agrarias; ArgentinaFil: Hernández Guijarro, Keren. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce. Unidad Integrada. Universidad Nacional de Mar del Plata. Facultad de Ciencias Agrarias; ArgentinaFil: Eyherabide, Mercedes. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce. Unidad Integrada. Universidad Nacional de Mar del Plata. Facultad de Ciencias Agrarias; Argentina.Fil: Sainz Rozas, Hernan Rene. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce. Unidad Integrada. Universidad Nacional de Mar del Plata. Facultad de Ciencias Agrarias; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Echeverria, Hernan Eduardo. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce. Unidad Integrada. Universidad Nacional de Mar del Plata. Facultad de Ciencias Agrarias; ArgentinaFil: Covacevich, Fernanda. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce. Unidad Integrada. Universidad Nacional de Mar del Plata. Facultad de Ciencias Agrarias; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Mar del Plata. Instituto de Investigaciones en Biodiversidad y Biotecnología; Argentina. Fundación para Investigaciones Biológicas Aplicadas; ArgentinaElsevier2019-06-10T15:17:23Z2019-06-10T15:17:23Z2016-05info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://hdl.handle.net/20.500.12123/5288https://www.sciencedirect.com/science/article/pii/S09291393163000510929-1393https://doi.org/10.1016/j.apsoil.2016.01.005Applied Soil Ecology 101 : 47-56 (May 2016)reponame:INTA Digital (INTA)instname:Instituto Nacional de Tecnología Agropecuariaenginfo:eu-repo/semantics/restrictedAccess2025-10-23T11:16:58Zoai:localhost:20.500.12123/5288instacron:INTAInstitucionalhttp://repositorio.inta.gob.ar/Organismo científico-tecnológicoNo correspondehttp://repositorio.inta.gob.ar/oai/requesttripaldi.nicolas@inta.gob.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:l2025-10-23 11:16:58.973INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuariafalse
dc.title.none.fl_str_mv Can soil properties and agricultural land use affect arbuscular mycorrhizal fungal communities indigenous from the Argentinean Pampas soils?
title Can soil properties and agricultural land use affect arbuscular mycorrhizal fungal communities indigenous from the Argentinean Pampas soils?
spellingShingle Can soil properties and agricultural land use affect arbuscular mycorrhizal fungal communities indigenous from the Argentinean Pampas soils?
Thougnon Islas, Andrea Julieta
Suelo
Propiedades Físico - Químicas Suelo
Utilización de la Tierra
Micorrizas Arbusculares
Hongos
Soil
Soil Chemicophysical Properties
Land Use
Arbuscular Mycorrhiza
Fungi
Región Pampeana
title_short Can soil properties and agricultural land use affect arbuscular mycorrhizal fungal communities indigenous from the Argentinean Pampas soils?
title_full Can soil properties and agricultural land use affect arbuscular mycorrhizal fungal communities indigenous from the Argentinean Pampas soils?
title_fullStr Can soil properties and agricultural land use affect arbuscular mycorrhizal fungal communities indigenous from the Argentinean Pampas soils?
title_full_unstemmed Can soil properties and agricultural land use affect arbuscular mycorrhizal fungal communities indigenous from the Argentinean Pampas soils?
title_sort Can soil properties and agricultural land use affect arbuscular mycorrhizal fungal communities indigenous from the Argentinean Pampas soils?
dc.creator.none.fl_str_mv Thougnon Islas, Andrea Julieta
Hernandez Guijarro, Keren
Eyherabide, Mercedes
Sainz Rozas, Hernan Rene
Echeverria, Hernan Eduardo
Covacevich, Fernanda
author Thougnon Islas, Andrea Julieta
author_facet Thougnon Islas, Andrea Julieta
Hernandez Guijarro, Keren
Eyherabide, Mercedes
Sainz Rozas, Hernan Rene
Echeverria, Hernan Eduardo
Covacevich, Fernanda
author_role author
author2 Hernandez Guijarro, Keren
Eyherabide, Mercedes
Sainz Rozas, Hernan Rene
Echeverria, Hernan Eduardo
Covacevich, Fernanda
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv Suelo
Propiedades Físico - Químicas Suelo
Utilización de la Tierra
Micorrizas Arbusculares
Hongos
Soil
Soil Chemicophysical Properties
Land Use
Arbuscular Mycorrhiza
Fungi
Región Pampeana
topic Suelo
Propiedades Físico - Químicas Suelo
Utilización de la Tierra
Micorrizas Arbusculares
Hongos
Soil
Soil Chemicophysical Properties
Land Use
Arbuscular Mycorrhiza
Fungi
Región Pampeana
dc.description.none.fl_txt_mv In order to determine if intensive agricultural land use and/or edaphic characteristics modify the community of the arbuscular mycorrhizal fungi (AMF) indigenous of soils from the Argentinean Pampa; mycorrhizal colonization, sporulation, glomalin (GRSP) and genetic diversity were assessed. Soils were collected from seven localities from Buenos Aires Province (Argentina) under contrasting land use intensity (at each site both pristine and agricultural soils). The capacity for root colonization, sporulation and glomalin content of trap plants and growth substrate were considered as measure of AMF activity. Analysis of diversity was conducted using PCR-single strand conformation polymorphism (SSCP). Profiles were generated by using primers to amplify the 28S rDNA of AMF from rDNA extracted directly from the substrate of trap plant or AMF spore. Five bands of the SSCP gel were sequenced and showed similarity with the genus Glomus. Analysis of colonization and GRSP content, were not clearly related to the land use intensity but sporulation capacity was, in general, lower in agricultural soils than in pristine ones. Soil phosphorus, ferrum and zinc contents, were negatively related to the root colonization and sporulation capacities. High genetic diversity was found both from soil and spore samples. Although similar diversity was found under agricultural and pristine soils, in most sites SSCP-diversity was separately clustered by land use. Still remains clarify if soil characteristics, resulting from agricultural management; exert selection pressure on the AMF and whether they could be vectors of interest to select potential plant growth promoting microorganisms.
EEA Balcarce
Fil: Thougnon Islas, Andrea Julieta. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce. Unidad Integrada. Universidad Nacional de Mar del Plata. Facultad de Ciencias Agrarias; Argentina
Fil: Hernández Guijarro, Keren. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce. Unidad Integrada. Universidad Nacional de Mar del Plata. Facultad de Ciencias Agrarias; Argentina
Fil: Eyherabide, Mercedes. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce. Unidad Integrada. Universidad Nacional de Mar del Plata. Facultad de Ciencias Agrarias; Argentina.
Fil: Sainz Rozas, Hernan Rene. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce. Unidad Integrada. Universidad Nacional de Mar del Plata. Facultad de Ciencias Agrarias; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Echeverria, Hernan Eduardo. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce. Unidad Integrada. Universidad Nacional de Mar del Plata. Facultad de Ciencias Agrarias; Argentina
Fil: Covacevich, Fernanda. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce. Unidad Integrada. Universidad Nacional de Mar del Plata. Facultad de Ciencias Agrarias; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Mar del Plata. Instituto de Investigaciones en Biodiversidad y Biotecnología; Argentina. Fundación para Investigaciones Biológicas Aplicadas; Argentina
description In order to determine if intensive agricultural land use and/or edaphic characteristics modify the community of the arbuscular mycorrhizal fungi (AMF) indigenous of soils from the Argentinean Pampa; mycorrhizal colonization, sporulation, glomalin (GRSP) and genetic diversity were assessed. Soils were collected from seven localities from Buenos Aires Province (Argentina) under contrasting land use intensity (at each site both pristine and agricultural soils). The capacity for root colonization, sporulation and glomalin content of trap plants and growth substrate were considered as measure of AMF activity. Analysis of diversity was conducted using PCR-single strand conformation polymorphism (SSCP). Profiles were generated by using primers to amplify the 28S rDNA of AMF from rDNA extracted directly from the substrate of trap plant or AMF spore. Five bands of the SSCP gel were sequenced and showed similarity with the genus Glomus. Analysis of colonization and GRSP content, were not clearly related to the land use intensity but sporulation capacity was, in general, lower in agricultural soils than in pristine ones. Soil phosphorus, ferrum and zinc contents, were negatively related to the root colonization and sporulation capacities. High genetic diversity was found both from soil and spore samples. Although similar diversity was found under agricultural and pristine soils, in most sites SSCP-diversity was separately clustered by land use. Still remains clarify if soil characteristics, resulting from agricultural management; exert selection pressure on the AMF and whether they could be vectors of interest to select potential plant growth promoting microorganisms.
publishDate 2016
dc.date.none.fl_str_mv 2016-05
2019-06-10T15:17:23Z
2019-06-10T15:17:23Z
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/20.500.12123/5288
https://www.sciencedirect.com/science/article/pii/S0929139316300051
0929-1393
https://doi.org/10.1016/j.apsoil.2016.01.005
url http://hdl.handle.net/20.500.12123/5288
https://www.sciencedirect.com/science/article/pii/S0929139316300051
https://doi.org/10.1016/j.apsoil.2016.01.005
identifier_str_mv 0929-1393
dc.language.none.fl_str_mv eng
language eng
dc.rights.none.fl_str_mv info:eu-repo/semantics/restrictedAccess
eu_rights_str_mv restrictedAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv Applied Soil Ecology 101 : 47-56 (May 2016)
reponame:INTA Digital (INTA)
instname:Instituto Nacional de Tecnología Agropecuaria
reponame_str INTA Digital (INTA)
collection INTA Digital (INTA)
instname_str Instituto Nacional de Tecnología Agropecuaria
repository.name.fl_str_mv INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuaria
repository.mail.fl_str_mv tripaldi.nicolas@inta.gob.ar
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score 12.982451

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