An In Vitro Method for Studying the Three-Way Interaction between Soybean, Rhizophagus irregularis and the Soil-Borne Pathogen Fusarium virguliforme

Autores
Giachero, Marí­a Lorena; Marquez, Nathalie; Gallou, Adrien; Luna, Celina Mercedes; Declerck, Stéphane; Ducasse, Daniel Adrian
Año de publicación
2017
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
In this work, we described an in vitro system adequate for investigating the pathosystem soybean/arbuscular mycorrhizal fungi (AMF)/Fusarium virguliforme. Pre-mycorrhized plantlets with Rhizophagus irregularis were infected by F. virguliforme either locally via a plug of gel supporting mycelium (Method 1) or via a macroconidia suspension applied to the medium surface (Method 2). Root colonization by the AMF and infection by the pathogen were similar to the usual observations in pot experiments. Within a period of 18 days, more than 20% of the roots were colonized by the AMF and infection by the pathogen was observed in all the plants. In presence of AMF, a decrease in symptoms and in the level of root tissue infection was noticed. With Method 1, smaller necrotic lesions were observed in the pre-mycorrhized plantlets. In Method 2, pathogen infection was slower but more homogenous. These results demonstrated the suitability of the in vitro cultivation system to study the pathosystem soybean/AMF/F. virguliforme. We propose this in vitro cultivation system for studying the mechanisms involved in the biocontrol conferred by AMF against F. virguliforme in soybean.
Instituto de Patología Vegetal
Instituto de Fisiología y Recursos Genéticos Vegetales
Fil: Giachero, Marí­a Lorena. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina
Fil: Marquez, Nathalie. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Gallou, Adrien. Centro Nacional de Referencia de Control Biológico; México
Fil: Luna, Celina Mercedes. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Fisiología y Recursos Genéticos Vegetales; Argentina
Fil: Declerck, Stéphane. Université Catholique de Louvain. Earth and Life Institute, Applied Microbiology, Mycology; Bélgica
Fil: Ducasse, Daniel Adrian. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina
Fuente
Frontiers in Plant Science 8 : 1033 (June 2017)
Materia
Soja
Rhizophagus
Fusarium
Patógenos
Suelo
Organismos Transmitidos por Suelo
Soybeans
Pathogens
Soil
Soilborne Organisms
In Vitro
Rhizophagus irregularis
Fusarium virguliforme
Nivel de accesibilidad
acceso abierto
Condiciones de uso
http://creativecommons.org/licenses/by-nc-sa/4.0/
Repositorio
INTA Digital (INTA)
Institución
Instituto Nacional de Tecnología Agropecuaria
OAI Identificador
oai:localhost:20.500.12123/2238
Acceder
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oai_identifier_str oai:localhost:20.500.12123/2238
network_acronym_str INTADig
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network_name_str INTA Digital (INTA)
spelling An In Vitro Method for Studying the Three-Way Interaction between Soybean, Rhizophagus irregularis and the Soil-Borne Pathogen Fusarium virguliformeGiachero, Marí­a LorenaMarquez, NathalieGallou, AdrienLuna, Celina MercedesDeclerck, StéphaneDucasse, Daniel AdrianSojaRhizophagusFusariumPatógenosSueloOrganismos Transmitidos por SueloSoybeansPathogensSoilSoilborne OrganismsIn VitroRhizophagus irregularisFusarium virguliformeIn this work, we described an in vitro system adequate for investigating the pathosystem soybean/arbuscular mycorrhizal fungi (AMF)/Fusarium virguliforme. Pre-mycorrhized plantlets with Rhizophagus irregularis were infected by F. virguliforme either locally via a plug of gel supporting mycelium (Method 1) or via a macroconidia suspension applied to the medium surface (Method 2). Root colonization by the AMF and infection by the pathogen were similar to the usual observations in pot experiments. Within a period of 18 days, more than 20% of the roots were colonized by the AMF and infection by the pathogen was observed in all the plants. In presence of AMF, a decrease in symptoms and in the level of root tissue infection was noticed. With Method 1, smaller necrotic lesions were observed in the pre-mycorrhized plantlets. In Method 2, pathogen infection was slower but more homogenous. These results demonstrated the suitability of the in vitro cultivation system to study the pathosystem soybean/AMF/F. virguliforme. We propose this in vitro cultivation system for studying the mechanisms involved in the biocontrol conferred by AMF against F. virguliforme in soybean.Instituto de Patología VegetalInstituto de Fisiología y Recursos Genéticos VegetalesFil: Giachero, Marí­a Lorena. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; ArgentinaFil: Marquez, Nathalie. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Gallou, Adrien. Centro Nacional de Referencia de Control Biológico; MéxicoFil: Luna, Celina Mercedes. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Fisiología y Recursos Genéticos Vegetales; ArgentinaFil: Declerck, Stéphane. Université Catholique de Louvain. Earth and Life Institute, Applied Microbiology, Mycology; BélgicaFil: Ducasse, Daniel Adrian. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina2018-04-13T11:41:53Z2018-04-13T11:41:53Z2017-06info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttps://www.frontiersin.org/articles/10.3389/fpls.2017.01033/fullhttp://hdl.handle.net/20.500.12123/22381664-462Xhttps://doi.org/10.3389/fpls.2017.01033Frontiers in Plant Science 8 : 1033 (June 2017)reponame:INTA Digital (INTA)instname:Instituto Nacional de Tecnología Agropecuariaenginfo:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by-nc-sa/4.0/Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)2025-10-16T09:29:09Zoai:localhost:20.500.12123/2238instacron:INTAInstitucionalhttp://repositorio.inta.gob.ar/Organismo científico-tecnológicoNo correspondehttp://repositorio.inta.gob.ar/oai/requesttripaldi.nicolas@inta.gob.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:l2025-10-16 09:29:09.604INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuariafalse
dc.title.none.fl_str_mv An In Vitro Method for Studying the Three-Way Interaction between Soybean, Rhizophagus irregularis and the Soil-Borne Pathogen Fusarium virguliforme
title An In Vitro Method for Studying the Three-Way Interaction between Soybean, Rhizophagus irregularis and the Soil-Borne Pathogen Fusarium virguliforme
spellingShingle An In Vitro Method for Studying the Three-Way Interaction between Soybean, Rhizophagus irregularis and the Soil-Borne Pathogen Fusarium virguliforme
Giachero, Marí­a Lorena
Soja
Rhizophagus
Fusarium
Patógenos
Suelo
Organismos Transmitidos por Suelo
Soybeans
Pathogens
Soil
Soilborne Organisms
In Vitro
Rhizophagus irregularis
Fusarium virguliforme
title_short An In Vitro Method for Studying the Three-Way Interaction between Soybean, Rhizophagus irregularis and the Soil-Borne Pathogen Fusarium virguliforme
title_full An In Vitro Method for Studying the Three-Way Interaction between Soybean, Rhizophagus irregularis and the Soil-Borne Pathogen Fusarium virguliforme
title_fullStr An In Vitro Method for Studying the Three-Way Interaction between Soybean, Rhizophagus irregularis and the Soil-Borne Pathogen Fusarium virguliforme
title_full_unstemmed An In Vitro Method for Studying the Three-Way Interaction between Soybean, Rhizophagus irregularis and the Soil-Borne Pathogen Fusarium virguliforme
title_sort An In Vitro Method for Studying the Three-Way Interaction between Soybean, Rhizophagus irregularis and the Soil-Borne Pathogen Fusarium virguliforme
dc.creator.none.fl_str_mv Giachero, Marí­a Lorena
Marquez, Nathalie
Gallou, Adrien
Luna, Celina Mercedes
Declerck, Stéphane
Ducasse, Daniel Adrian
author Giachero, Marí­a Lorena
author_facet Giachero, Marí­a Lorena
Marquez, Nathalie
Gallou, Adrien
Luna, Celina Mercedes
Declerck, Stéphane
Ducasse, Daniel Adrian
author_role author
author2 Marquez, Nathalie
Gallou, Adrien
Luna, Celina Mercedes
Declerck, Stéphane
Ducasse, Daniel Adrian
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv Soja
Rhizophagus
Fusarium
Patógenos
Suelo
Organismos Transmitidos por Suelo
Soybeans
Pathogens
Soil
Soilborne Organisms
In Vitro
Rhizophagus irregularis
Fusarium virguliforme
topic Soja
Rhizophagus
Fusarium
Patógenos
Suelo
Organismos Transmitidos por Suelo
Soybeans
Pathogens
Soil
Soilborne Organisms
In Vitro
Rhizophagus irregularis
Fusarium virguliforme
dc.description.none.fl_txt_mv In this work, we described an in vitro system adequate for investigating the pathosystem soybean/arbuscular mycorrhizal fungi (AMF)/Fusarium virguliforme. Pre-mycorrhized plantlets with Rhizophagus irregularis were infected by F. virguliforme either locally via a plug of gel supporting mycelium (Method 1) or via a macroconidia suspension applied to the medium surface (Method 2). Root colonization by the AMF and infection by the pathogen were similar to the usual observations in pot experiments. Within a period of 18 days, more than 20% of the roots were colonized by the AMF and infection by the pathogen was observed in all the plants. In presence of AMF, a decrease in symptoms and in the level of root tissue infection was noticed. With Method 1, smaller necrotic lesions were observed in the pre-mycorrhized plantlets. In Method 2, pathogen infection was slower but more homogenous. These results demonstrated the suitability of the in vitro cultivation system to study the pathosystem soybean/AMF/F. virguliforme. We propose this in vitro cultivation system for studying the mechanisms involved in the biocontrol conferred by AMF against F. virguliforme in soybean.
Instituto de Patología Vegetal
Instituto de Fisiología y Recursos Genéticos Vegetales
Fil: Giachero, Marí­a Lorena. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina
Fil: Marquez, Nathalie. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Gallou, Adrien. Centro Nacional de Referencia de Control Biológico; México
Fil: Luna, Celina Mercedes. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Fisiología y Recursos Genéticos Vegetales; Argentina
Fil: Declerck, Stéphane. Université Catholique de Louvain. Earth and Life Institute, Applied Microbiology, Mycology; Bélgica
Fil: Ducasse, Daniel Adrian. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina
description In this work, we described an in vitro system adequate for investigating the pathosystem soybean/arbuscular mycorrhizal fungi (AMF)/Fusarium virguliforme. Pre-mycorrhized plantlets with Rhizophagus irregularis were infected by F. virguliforme either locally via a plug of gel supporting mycelium (Method 1) or via a macroconidia suspension applied to the medium surface (Method 2). Root colonization by the AMF and infection by the pathogen were similar to the usual observations in pot experiments. Within a period of 18 days, more than 20% of the roots were colonized by the AMF and infection by the pathogen was observed in all the plants. In presence of AMF, a decrease in symptoms and in the level of root tissue infection was noticed. With Method 1, smaller necrotic lesions were observed in the pre-mycorrhized plantlets. In Method 2, pathogen infection was slower but more homogenous. These results demonstrated the suitability of the in vitro cultivation system to study the pathosystem soybean/AMF/F. virguliforme. We propose this in vitro cultivation system for studying the mechanisms involved in the biocontrol conferred by AMF against F. virguliforme in soybean.
publishDate 2017
dc.date.none.fl_str_mv 2017-06
2018-04-13T11:41:53Z
2018-04-13T11:41:53Z
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv https://www.frontiersin.org/articles/10.3389/fpls.2017.01033/full
http://hdl.handle.net/20.500.12123/2238
1664-462X
https://doi.org/10.3389/fpls.2017.01033
url https://www.frontiersin.org/articles/10.3389/fpls.2017.01033/full
http://hdl.handle.net/20.500.12123/2238
https://doi.org/10.3389/fpls.2017.01033
identifier_str_mv 1664-462X
dc.language.none.fl_str_mv eng
language eng
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
eu_rights_str_mv openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv Frontiers in Plant Science 8 : 1033 (June 2017)
reponame:INTA Digital (INTA)
instname:Instituto Nacional de Tecnología Agropecuaria
reponame_str INTA Digital (INTA)
collection INTA Digital (INTA)
instname_str Instituto Nacional de Tecnología Agropecuaria
repository.name.fl_str_mv INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuaria
repository.mail.fl_str_mv tripaldi.nicolas@inta.gob.ar
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score 12.712165

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