Clade 8 and clade 6 strains of Escherichia coli O157:H7 from cattle in Argentina have Hypervirulent-Like phenotypes

Autores
Amigo, Natalia; Mercado, Elsa Cristina; Bentancor, Adriana Beatriz; Singh, Pallavi; Vilte, Daniel Alejandro; Gerhardt, Elizabeth; Zotta, Elsa; Ibarra, Cristina Adriana; Manning, Shannon D.; Larzabal, Mariano; Cataldi, Angel Adrian
Año de publicación
2015
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The hemolytic uremic syndrome (HUS) whose main causative agent is enterohemorrhagic Escherichia coli (EHEC) O157:H7 is a disease that mainly affects children under 5 years of age. Argentina is the country with the highest incidence of HUS in the world. Cattle are a major reservoir and source of infection with E. coli O157:H7. To date, the epidemiological factors that contribute to its prevalence are poorly understood. Single nucleotide polymorphism (SNP) typing has helped to define nine E. coli O157:H7 clades and the clade 8 strains were associated with most of the cases of severe disease. In this study, eight randomly selected isolates of EHEC O157:H7 from cattle in Argentina were studied as well as two human isolates. Four of them were classified as clade 8 through the screening for 23 SNPs; the two human isolates grouped in this clade as well, while two strains were closely related to strains representing clade 6. To assess the pathogenicity of these strains, we assayed correlates of virulence. Shiga toxin production was determined by an ELISA kit. Four strains were high producers and one of these strains that belonged to a novel genotype showed high verocytotoxic activity in cultured cells. Also, these clade 8 and 6 strains showed high RBC lysis and adherence to epithelial cells. One of the clade 6 strains showed stronger inhibition of normal water absorption than E. coli O157:H7 EDL933 in human colonic explants. In addition, two of the strains showing high levels of Stx2 production and RBC lysis activity were associated with lethality and uremia in a mouse model. Consequently, circulation of such strains in cattle may partially contribute to the high incidence of HUS in Argentina.
Instituto de Biotecnología
Fil: Amigo, Natalia. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina
Fil: Mercado, Elsa Cristina. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiologia; Argentina
Fil: Bentancor, Adriana Beatriz. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias. Microbiología; Argentina
Fil: Singh, Pallavi. Michigan State University. Department of Microbiology and Molecular Genetics; Estados Unidos
Fil: Vilte, Daniel Alejandro. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiologia; Argentina
Fil: Gerhardt, Elizabeth. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Fisiología y Biofísica Bernardo Houssay. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Fisiología y Biofísica Bernardo Houssay; Argentina. Universidad de Buenos Aires. Facultad de Medicina. Departamento de Ciencias Fisiológicas; Argentina
Fil: Zotta, Elsa. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Fisiología y Biofísica Bernardo Houssay. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Fisiología y Biofísica Bernardo Houssay; Argentina. Universidad de Buenos Aires. Facultad de Medicina. Departamento de Ciencias Fisiológicas; Argentina
Fil: Ibarra, Cristina Adriana. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Fisiología y Biofísica Bernardo Houssay. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Fisiología y Biofísica Bernardo Houssay; Argentina. Universidad de Buenos Aires. Facultad de Medicina. Departamento de Ciencias Fisiológicas; Argentina
Fil: Manning, Shannon D. Michigan State University. Department of Microbiology and Molecular Genetics; Estados Unidos
Fil: Larzabal, Mariano. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina
Fil: Cataldi, Angel Adrian. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina
Fuente
PLoS ONE 10 (6) : e0127710. (June 1, 2015)
Materia
Escherichia Coli
Ganado Bovino
Fenotipos
Genética Molecular
Colon (Intestino)
Cattle
Phenotypes
Molecular Genetics
Colon
Hemolytic-uremic Syndrome
Síndrome Urémico Hemolítico
Argentina
Nivel de accesibilidad
acceso abierto
Condiciones de uso
http://creativecommons.org/licenses/by-nc-sa/4.0/
Repositorio
INTA Digital (INTA)
Institución
Instituto Nacional de Tecnología Agropecuaria
OAI Identificador
oai:localhost:20.500.12123/4764
Acceder
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oai_identifier_str oai:localhost:20.500.12123/4764
network_acronym_str INTADig
repository_id_str l
network_name_str INTA Digital (INTA)
spelling Clade 8 and clade 6 strains of Escherichia coli O157:H7 from cattle in Argentina have Hypervirulent-Like phenotypesAmigo, NataliaMercado, Elsa CristinaBentancor, Adriana BeatrizSingh, PallaviVilte, Daniel AlejandroGerhardt, ElizabethZotta, ElsaIbarra, Cristina AdrianaManning, Shannon D.Larzabal, MarianoCataldi, Angel AdrianEscherichia ColiGanado BovinoFenotiposGenética MolecularColon (Intestino)CattlePhenotypesMolecular GeneticsColonHemolytic-uremic SyndromeSíndrome Urémico HemolíticoArgentinaThe hemolytic uremic syndrome (HUS) whose main causative agent is enterohemorrhagic Escherichia coli (EHEC) O157:H7 is a disease that mainly affects children under 5 years of age. Argentina is the country with the highest incidence of HUS in the world. Cattle are a major reservoir and source of infection with E. coli O157:H7. To date, the epidemiological factors that contribute to its prevalence are poorly understood. Single nucleotide polymorphism (SNP) typing has helped to define nine E. coli O157:H7 clades and the clade 8 strains were associated with most of the cases of severe disease. In this study, eight randomly selected isolates of EHEC O157:H7 from cattle in Argentina were studied as well as two human isolates. Four of them were classified as clade 8 through the screening for 23 SNPs; the two human isolates grouped in this clade as well, while two strains were closely related to strains representing clade 6. To assess the pathogenicity of these strains, we assayed correlates of virulence. Shiga toxin production was determined by an ELISA kit. Four strains were high producers and one of these strains that belonged to a novel genotype showed high verocytotoxic activity in cultured cells. Also, these clade 8 and 6 strains showed high RBC lysis and adherence to epithelial cells. One of the clade 6 strains showed stronger inhibition of normal water absorption than E. coli O157:H7 EDL933 in human colonic explants. In addition, two of the strains showing high levels of Stx2 production and RBC lysis activity were associated with lethality and uremia in a mouse model. Consequently, circulation of such strains in cattle may partially contribute to the high incidence of HUS in Argentina.Instituto de BiotecnologíaFil: Amigo, Natalia. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; ArgentinaFil: Mercado, Elsa Cristina. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiologia; ArgentinaFil: Bentancor, Adriana Beatriz. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias. Microbiología; ArgentinaFil: Singh, Pallavi. Michigan State University. Department of Microbiology and Molecular Genetics; Estados UnidosFil: Vilte, Daniel Alejandro. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiologia; ArgentinaFil: Gerhardt, Elizabeth. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Fisiología y Biofísica Bernardo Houssay. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Fisiología y Biofísica Bernardo Houssay; Argentina. Universidad de Buenos Aires. Facultad de Medicina. Departamento de Ciencias Fisiológicas; ArgentinaFil: Zotta, Elsa. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Fisiología y Biofísica Bernardo Houssay. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Fisiología y Biofísica Bernardo Houssay; Argentina. Universidad de Buenos Aires. Facultad de Medicina. Departamento de Ciencias Fisiológicas; ArgentinaFil: Ibarra, Cristina Adriana. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Fisiología y Biofísica Bernardo Houssay. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Fisiología y Biofísica Bernardo Houssay; Argentina. Universidad de Buenos Aires. Facultad de Medicina. Departamento de Ciencias Fisiológicas; ArgentinaFil: Manning, Shannon D. Michigan State University. Department of Microbiology and Molecular Genetics; Estados UnidosFil: Larzabal, Mariano. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; ArgentinaFil: Cataldi, Angel Adrian. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; ArgentinaPLOS2019-03-27T17:45:08Z2019-03-27T17:45:08Z2015-06-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://hdl.handle.net/20.500.12123/4764https://journals.plos.org/plosone/article?id=10.1371/journal.pone.01277101932-6203https://doi.org/10.1371/journal.pone.0127710PLoS ONE 10 (6) : e0127710. (June 1, 2015)reponame:INTA Digital (INTA)instname:Instituto Nacional de Tecnología Agropecuariaenginfo:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by-nc-sa/4.0/Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)2025-10-16T09:29:29Zoai:localhost:20.500.12123/4764instacron:INTAInstitucionalhttp://repositorio.inta.gob.ar/Organismo científico-tecnológicoNo correspondehttp://repositorio.inta.gob.ar/oai/requesttripaldi.nicolas@inta.gob.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:l2025-10-16 09:29:29.575INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuariafalse
dc.title.none.fl_str_mv Clade 8 and clade 6 strains of Escherichia coli O157:H7 from cattle in Argentina have Hypervirulent-Like phenotypes
title Clade 8 and clade 6 strains of Escherichia coli O157:H7 from cattle in Argentina have Hypervirulent-Like phenotypes
spellingShingle Clade 8 and clade 6 strains of Escherichia coli O157:H7 from cattle in Argentina have Hypervirulent-Like phenotypes
Amigo, Natalia
Escherichia Coli
Ganado Bovino
Fenotipos
Genética Molecular
Colon (Intestino)
Cattle
Phenotypes
Molecular Genetics
Colon
Hemolytic-uremic Syndrome
Síndrome Urémico Hemolítico
Argentina
title_short Clade 8 and clade 6 strains of Escherichia coli O157:H7 from cattle in Argentina have Hypervirulent-Like phenotypes
title_full Clade 8 and clade 6 strains of Escherichia coli O157:H7 from cattle in Argentina have Hypervirulent-Like phenotypes
title_fullStr Clade 8 and clade 6 strains of Escherichia coli O157:H7 from cattle in Argentina have Hypervirulent-Like phenotypes
title_full_unstemmed Clade 8 and clade 6 strains of Escherichia coli O157:H7 from cattle in Argentina have Hypervirulent-Like phenotypes
title_sort Clade 8 and clade 6 strains of Escherichia coli O157:H7 from cattle in Argentina have Hypervirulent-Like phenotypes
dc.creator.none.fl_str_mv Amigo, Natalia
Mercado, Elsa Cristina
Bentancor, Adriana Beatriz
Singh, Pallavi
Vilte, Daniel Alejandro
Gerhardt, Elizabeth
Zotta, Elsa
Ibarra, Cristina Adriana
Manning, Shannon D.
Larzabal, Mariano
Cataldi, Angel Adrian
author Amigo, Natalia
author_facet Amigo, Natalia
Mercado, Elsa Cristina
Bentancor, Adriana Beatriz
Singh, Pallavi
Vilte, Daniel Alejandro
Gerhardt, Elizabeth
Zotta, Elsa
Ibarra, Cristina Adriana
Manning, Shannon D.
Larzabal, Mariano
Cataldi, Angel Adrian
author_role author
author2 Mercado, Elsa Cristina
Bentancor, Adriana Beatriz
Singh, Pallavi
Vilte, Daniel Alejandro
Gerhardt, Elizabeth
Zotta, Elsa
Ibarra, Cristina Adriana
Manning, Shannon D.
Larzabal, Mariano
Cataldi, Angel Adrian
author2_role author
author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Escherichia Coli
Ganado Bovino
Fenotipos
Genética Molecular
Colon (Intestino)
Cattle
Phenotypes
Molecular Genetics
Colon
Hemolytic-uremic Syndrome
Síndrome Urémico Hemolítico
Argentina
topic Escherichia Coli
Ganado Bovino
Fenotipos
Genética Molecular
Colon (Intestino)
Cattle
Phenotypes
Molecular Genetics
Colon
Hemolytic-uremic Syndrome
Síndrome Urémico Hemolítico
Argentina
dc.description.none.fl_txt_mv The hemolytic uremic syndrome (HUS) whose main causative agent is enterohemorrhagic Escherichia coli (EHEC) O157:H7 is a disease that mainly affects children under 5 years of age. Argentina is the country with the highest incidence of HUS in the world. Cattle are a major reservoir and source of infection with E. coli O157:H7. To date, the epidemiological factors that contribute to its prevalence are poorly understood. Single nucleotide polymorphism (SNP) typing has helped to define nine E. coli O157:H7 clades and the clade 8 strains were associated with most of the cases of severe disease. In this study, eight randomly selected isolates of EHEC O157:H7 from cattle in Argentina were studied as well as two human isolates. Four of them were classified as clade 8 through the screening for 23 SNPs; the two human isolates grouped in this clade as well, while two strains were closely related to strains representing clade 6. To assess the pathogenicity of these strains, we assayed correlates of virulence. Shiga toxin production was determined by an ELISA kit. Four strains were high producers and one of these strains that belonged to a novel genotype showed high verocytotoxic activity in cultured cells. Also, these clade 8 and 6 strains showed high RBC lysis and adherence to epithelial cells. One of the clade 6 strains showed stronger inhibition of normal water absorption than E. coli O157:H7 EDL933 in human colonic explants. In addition, two of the strains showing high levels of Stx2 production and RBC lysis activity were associated with lethality and uremia in a mouse model. Consequently, circulation of such strains in cattle may partially contribute to the high incidence of HUS in Argentina.
Instituto de Biotecnología
Fil: Amigo, Natalia. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina
Fil: Mercado, Elsa Cristina. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiologia; Argentina
Fil: Bentancor, Adriana Beatriz. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias. Microbiología; Argentina
Fil: Singh, Pallavi. Michigan State University. Department of Microbiology and Molecular Genetics; Estados Unidos
Fil: Vilte, Daniel Alejandro. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiologia; Argentina
Fil: Gerhardt, Elizabeth. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Fisiología y Biofísica Bernardo Houssay. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Fisiología y Biofísica Bernardo Houssay; Argentina. Universidad de Buenos Aires. Facultad de Medicina. Departamento de Ciencias Fisiológicas; Argentina
Fil: Zotta, Elsa. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Fisiología y Biofísica Bernardo Houssay. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Fisiología y Biofísica Bernardo Houssay; Argentina. Universidad de Buenos Aires. Facultad de Medicina. Departamento de Ciencias Fisiológicas; Argentina
Fil: Ibarra, Cristina Adriana. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Fisiología y Biofísica Bernardo Houssay. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Fisiología y Biofísica Bernardo Houssay; Argentina. Universidad de Buenos Aires. Facultad de Medicina. Departamento de Ciencias Fisiológicas; Argentina
Fil: Manning, Shannon D. Michigan State University. Department of Microbiology and Molecular Genetics; Estados Unidos
Fil: Larzabal, Mariano. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina
Fil: Cataldi, Angel Adrian. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina
description The hemolytic uremic syndrome (HUS) whose main causative agent is enterohemorrhagic Escherichia coli (EHEC) O157:H7 is a disease that mainly affects children under 5 years of age. Argentina is the country with the highest incidence of HUS in the world. Cattle are a major reservoir and source of infection with E. coli O157:H7. To date, the epidemiological factors that contribute to its prevalence are poorly understood. Single nucleotide polymorphism (SNP) typing has helped to define nine E. coli O157:H7 clades and the clade 8 strains were associated with most of the cases of severe disease. In this study, eight randomly selected isolates of EHEC O157:H7 from cattle in Argentina were studied as well as two human isolates. Four of them were classified as clade 8 through the screening for 23 SNPs; the two human isolates grouped in this clade as well, while two strains were closely related to strains representing clade 6. To assess the pathogenicity of these strains, we assayed correlates of virulence. Shiga toxin production was determined by an ELISA kit. Four strains were high producers and one of these strains that belonged to a novel genotype showed high verocytotoxic activity in cultured cells. Also, these clade 8 and 6 strains showed high RBC lysis and adherence to epithelial cells. One of the clade 6 strains showed stronger inhibition of normal water absorption than E. coli O157:H7 EDL933 in human colonic explants. In addition, two of the strains showing high levels of Stx2 production and RBC lysis activity were associated with lethality and uremia in a mouse model. Consequently, circulation of such strains in cattle may partially contribute to the high incidence of HUS in Argentina.
publishDate 2015
dc.date.none.fl_str_mv 2015-06-01
2019-03-27T17:45:08Z
2019-03-27T17:45:08Z
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/20.500.12123/4764
https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0127710
1932-6203
https://doi.org/10.1371/journal.pone.0127710
url http://hdl.handle.net/20.500.12123/4764
https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0127710
https://doi.org/10.1371/journal.pone.0127710
identifier_str_mv 1932-6203
dc.language.none.fl_str_mv eng
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rights_invalid_str_mv http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv PLOS
publisher.none.fl_str_mv PLOS
dc.source.none.fl_str_mv PLoS ONE 10 (6) : e0127710. (June 1, 2015)
reponame:INTA Digital (INTA)
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instname_str Instituto Nacional de Tecnología Agropecuaria
repository.name.fl_str_mv INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuaria
repository.mail.fl_str_mv tripaldi.nicolas@inta.gob.ar
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