Molecular detection and phylogenetic analysis of Hepatozoon spp. in questing Ixodes ricinus ticks and rodents from Slovakia and Czech Republic

Autores
Hamšíkova, Zuzana; Silaghi, Cornelia; Rudolf, Ivo; Venclíková, Kristýna; Mahríkova, Lenka; Slovák, Mirko; Mendel, Jan; Blažejová, Hana; Berthová, Lenka; Kocianová, Elena; Hubálek, Zdeněk; Schnittger, Leonhard; Kazimírová, Mária
Año de publicación
2016
Idioma
inglés
Tipo de recurso
artículo
Estado
versión aceptada
Descripción
By amplification and sequencing of 18S rRNA gene fragments, Hepatozoon spp. DNA was detected in 0.08 % (4/5057) and 0.04 % (1/2473) of questing Ixodes ricinus ticks from Slovakia and Czech Republic, respectively. Hepatozoon spp. DNA was also detected in spleen and/or lungs of 4.45 % (27/606) of rodents from Slovakia. Prevalence of infection was significantly higher in Myodes glareolus (11.45 %) than in Apodemus spp. (0.28 %) (P < 0.001). Sequencing of 18S rRNA Hepatozoon spp. gene amplicons from I. ricinus showed 100 % identity with Hepatozoon canis isolates from red foxes or dogs in Europe. Phylogenetic analysis showed that at least two H. canis 18S rRNA genotypes exist in Slovakia of which one was identified also in the Czech Republic. The finding of H. canis in questing I. ricinus suggests the geographical spread of the parasite and a potential role of other ticks as its vectors in areas where Rhipicephalus sanguineus is not endemic. Sequencing of 18S rRNA gene amplicons from M. glareolus revealed the presence of two closely related genetic variants, Hepatozoon sp. SK1 and Hepatozoon sp. SK2, showing 99–100 % identity with isolates from M. glareolus from other European countries. Phylogenetic analysis demonstrates that 18S rRNA variants SK1 and SK2 correspond to previously described genotypes UR1 and UR2 of H. erhardovae, respectively. The isolate from Apodemus flavicollis (Hepatozoon sp. SK3b) was 99 % identical with isolates from reptiles in Africa and Asia. Further studies are necessary to identify the taxonomic status of Hepatozoon spp. parasitizing rodents in Europe and the host-parasite interactions in natural foci.
Fil: Hamšíková, Zuzana. Slovak Academy of Sciences. Institute of Zoology; Eslovaquia
Fil: Silaghi, Cornelia. Ludwig-Maximilians-Universität. Comparative Tropical Medicine and Parasitology; Alemania. University of Zurich. Institute of Parasitology. National Centre for Vector Entomology; Suiza
Fil: Rudolf, Ivo. The Czech Academy of Sciences. Institute of Vertebrate Biology; República Checa
Fil: Venclíková, Kristýna. The Czech Academy of Sciences. Institute of Vertebrate Biology; República Checa. The Czech Academy of Sciences. Institute of Macromolecular Chemistry; República Checa
Fil: Mahrikova, Lenka. Slovak Academy of Sciences. Institute of Zoology; Eslovaquia
Fil: Slovak, Mirko. Slovak Academy of Sciences. Institute of Zoology; Eslovaquia
Fil: Mendel, Jan. The Czech Academy of Sciences. Institute of Vertebrate Biology; República Checa.
Fil: Blažejová, Hana. The Czech Academy of Sciences. Institute of Vertebrate Biology; República Checa.
Fil: Berthova, Lenka. Slovak Academy of Sciences. Biomedical Research Center. Institute of Virology; Eslovaquia
Fil: Kocianova, Elena. Slovak Academy of Sciences. Biomedical Research Center. Institute of Virology; Eslovaquia
Fil: Hubálek, Zdeněk. The Czech Academy of Sciences. Institute of Vertebrate Biology; República Checa
Fil: Schnittger, Leonhard. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Kazimirová, Mária. Slovak Academy of Sciences. Institute of Zoology; Eslovaquia
Fuente
Parasitology research 115 (10) : 3897–3904. (October 2016)
Materia
Ixodes Ricinus
Metastigmata
Hepatozoon
Sporozoa
Roedores
Rodents
Garrapatas
Apicomplexa
Eslovaquia
República Checa
Nivel de accesibilidad
acceso restringido
Condiciones de uso
Repositorio
INTA Digital (INTA)
Institución
Instituto Nacional de Tecnología Agropecuaria
OAI Identificador
oai:localhost:20.500.12123/1347
Acceder
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oai_identifier_str oai:localhost:20.500.12123/1347
network_acronym_str INTADig
repository_id_str l
network_name_str INTA Digital (INTA)
spelling Molecular detection and phylogenetic analysis of Hepatozoon spp. in questing Ixodes ricinus ticks and rodents from Slovakia and Czech RepublicHamšíkova, ZuzanaSilaghi, CorneliaRudolf, IvoVenclíková, KristýnaMahríkova, LenkaSlovák, MirkoMendel, JanBlažejová, HanaBerthová, LenkaKocianová, ElenaHubálek, ZdeněkSchnittger, LeonhardKazimírová, MáriaIxodes RicinusMetastigmataHepatozoonSporozoaRoedoresRodentsGarrapatasApicomplexaEslovaquiaRepública ChecaBy amplification and sequencing of 18S rRNA gene fragments, Hepatozoon spp. DNA was detected in 0.08 % (4/5057) and 0.04 % (1/2473) of questing Ixodes ricinus ticks from Slovakia and Czech Republic, respectively. Hepatozoon spp. DNA was also detected in spleen and/or lungs of 4.45 % (27/606) of rodents from Slovakia. Prevalence of infection was significantly higher in Myodes glareolus (11.45 %) than in Apodemus spp. (0.28 %) (P < 0.001). Sequencing of 18S rRNA Hepatozoon spp. gene amplicons from I. ricinus showed 100 % identity with Hepatozoon canis isolates from red foxes or dogs in Europe. Phylogenetic analysis showed that at least two H. canis 18S rRNA genotypes exist in Slovakia of which one was identified also in the Czech Republic. The finding of H. canis in questing I. ricinus suggests the geographical spread of the parasite and a potential role of other ticks as its vectors in areas where Rhipicephalus sanguineus is not endemic. Sequencing of 18S rRNA gene amplicons from M. glareolus revealed the presence of two closely related genetic variants, Hepatozoon sp. SK1 and Hepatozoon sp. SK2, showing 99–100 % identity with isolates from M. glareolus from other European countries. Phylogenetic analysis demonstrates that 18S rRNA variants SK1 and SK2 correspond to previously described genotypes UR1 and UR2 of H. erhardovae, respectively. The isolate from Apodemus flavicollis (Hepatozoon sp. SK3b) was 99 % identical with isolates from reptiles in Africa and Asia. Further studies are necessary to identify the taxonomic status of Hepatozoon spp. parasitizing rodents in Europe and the host-parasite interactions in natural foci.Fil: Hamšíková, Zuzana. Slovak Academy of Sciences. Institute of Zoology; EslovaquiaFil: Silaghi, Cornelia. Ludwig-Maximilians-Universität. Comparative Tropical Medicine and Parasitology; Alemania. University of Zurich. Institute of Parasitology. National Centre for Vector Entomology; SuizaFil: Rudolf, Ivo. The Czech Academy of Sciences. Institute of Vertebrate Biology; República ChecaFil: Venclíková, Kristýna. The Czech Academy of Sciences. Institute of Vertebrate Biology; República Checa. The Czech Academy of Sciences. Institute of Macromolecular Chemistry; República ChecaFil: Mahrikova, Lenka. Slovak Academy of Sciences. Institute of Zoology; EslovaquiaFil: Slovak, Mirko. Slovak Academy of Sciences. Institute of Zoology; EslovaquiaFil: Mendel, Jan. The Czech Academy of Sciences. Institute of Vertebrate Biology; República Checa.Fil: Blažejová, Hana. The Czech Academy of Sciences. Institute of Vertebrate Biology; República Checa.Fil: Berthova, Lenka. Slovak Academy of Sciences. Biomedical Research Center. Institute of Virology; EslovaquiaFil: Kocianova, Elena. Slovak Academy of Sciences. Biomedical Research Center. Institute of Virology; EslovaquiaFil: Hubálek, Zdeněk. The Czech Academy of Sciences. Institute of Vertebrate Biology; República ChecaFil: Schnittger, Leonhard. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Kazimirová, Mária. Slovak Academy of Sciences. Institute of Zoology; Eslovaquia2017-09-28T13:48:52Z2017-09-28T13:48:52Z2016-10info:eu-repo/semantics/articleinfo:eu-repo/semantics/acceptedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://hdl.handle.net/20.500.12123/1347https://link.springer.com/article/10.1007%2Fs00436-016-5156-50932-0113 (Print)1432-1955 (Online)https://doi.org/10.1007/s00436-016-5156-5Parasitology research 115 (10) : 3897–3904. (October 2016)reponame:INTA Digital (INTA)instname:Instituto Nacional de Tecnología AgropecuariaengSlovakia (nation)Czech Republic (nation)info:eu-repo/semantics/restrictedAccess2025-09-29T13:44:11Zoai:localhost:20.500.12123/1347instacron:INTAInstitucionalhttp://repositorio.inta.gob.ar/Organismo científico-tecnológicoNo correspondehttp://repositorio.inta.gob.ar/oai/requesttripaldi.nicolas@inta.gob.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:l2025-09-29 13:44:11.722INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuariafalse
dc.title.none.fl_str_mv Molecular detection and phylogenetic analysis of Hepatozoon spp. in questing Ixodes ricinus ticks and rodents from Slovakia and Czech Republic
title Molecular detection and phylogenetic analysis of Hepatozoon spp. in questing Ixodes ricinus ticks and rodents from Slovakia and Czech Republic
spellingShingle Molecular detection and phylogenetic analysis of Hepatozoon spp. in questing Ixodes ricinus ticks and rodents from Slovakia and Czech Republic
Hamšíkova, Zuzana
Ixodes Ricinus
Metastigmata
Hepatozoon
Sporozoa
Roedores
Rodents
Garrapatas
Apicomplexa
Eslovaquia
República Checa
title_short Molecular detection and phylogenetic analysis of Hepatozoon spp. in questing Ixodes ricinus ticks and rodents from Slovakia and Czech Republic
title_full Molecular detection and phylogenetic analysis of Hepatozoon spp. in questing Ixodes ricinus ticks and rodents from Slovakia and Czech Republic
title_fullStr Molecular detection and phylogenetic analysis of Hepatozoon spp. in questing Ixodes ricinus ticks and rodents from Slovakia and Czech Republic
title_full_unstemmed Molecular detection and phylogenetic analysis of Hepatozoon spp. in questing Ixodes ricinus ticks and rodents from Slovakia and Czech Republic
title_sort Molecular detection and phylogenetic analysis of Hepatozoon spp. in questing Ixodes ricinus ticks and rodents from Slovakia and Czech Republic
dc.creator.none.fl_str_mv Hamšíkova, Zuzana
Silaghi, Cornelia
Rudolf, Ivo
Venclíková, Kristýna
Mahríkova, Lenka
Slovák, Mirko
Mendel, Jan
Blažejová, Hana
Berthová, Lenka
Kocianová, Elena
Hubálek, Zdeněk
Schnittger, Leonhard
Kazimírová, Mária
author Hamšíkova, Zuzana
author_facet Hamšíkova, Zuzana
Silaghi, Cornelia
Rudolf, Ivo
Venclíková, Kristýna
Mahríkova, Lenka
Slovák, Mirko
Mendel, Jan
Blažejová, Hana
Berthová, Lenka
Kocianová, Elena
Hubálek, Zdeněk
Schnittger, Leonhard
Kazimírová, Mária
author_role author
author2 Silaghi, Cornelia
Rudolf, Ivo
Venclíková, Kristýna
Mahríkova, Lenka
Slovák, Mirko
Mendel, Jan
Blažejová, Hana
Berthová, Lenka
Kocianová, Elena
Hubálek, Zdeněk
Schnittger, Leonhard
Kazimírová, Mária
author2_role author
author
author
author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Ixodes Ricinus
Metastigmata
Hepatozoon
Sporozoa
Roedores
Rodents
Garrapatas
Apicomplexa
Eslovaquia
República Checa
topic Ixodes Ricinus
Metastigmata
Hepatozoon
Sporozoa
Roedores
Rodents
Garrapatas
Apicomplexa
Eslovaquia
República Checa
dc.description.none.fl_txt_mv By amplification and sequencing of 18S rRNA gene fragments, Hepatozoon spp. DNA was detected in 0.08 % (4/5057) and 0.04 % (1/2473) of questing Ixodes ricinus ticks from Slovakia and Czech Republic, respectively. Hepatozoon spp. DNA was also detected in spleen and/or lungs of 4.45 % (27/606) of rodents from Slovakia. Prevalence of infection was significantly higher in Myodes glareolus (11.45 %) than in Apodemus spp. (0.28 %) (P < 0.001). Sequencing of 18S rRNA Hepatozoon spp. gene amplicons from I. ricinus showed 100 % identity with Hepatozoon canis isolates from red foxes or dogs in Europe. Phylogenetic analysis showed that at least two H. canis 18S rRNA genotypes exist in Slovakia of which one was identified also in the Czech Republic. The finding of H. canis in questing I. ricinus suggests the geographical spread of the parasite and a potential role of other ticks as its vectors in areas where Rhipicephalus sanguineus is not endemic. Sequencing of 18S rRNA gene amplicons from M. glareolus revealed the presence of two closely related genetic variants, Hepatozoon sp. SK1 and Hepatozoon sp. SK2, showing 99–100 % identity with isolates from M. glareolus from other European countries. Phylogenetic analysis demonstrates that 18S rRNA variants SK1 and SK2 correspond to previously described genotypes UR1 and UR2 of H. erhardovae, respectively. The isolate from Apodemus flavicollis (Hepatozoon sp. SK3b) was 99 % identical with isolates from reptiles in Africa and Asia. Further studies are necessary to identify the taxonomic status of Hepatozoon spp. parasitizing rodents in Europe and the host-parasite interactions in natural foci.
Fil: Hamšíková, Zuzana. Slovak Academy of Sciences. Institute of Zoology; Eslovaquia
Fil: Silaghi, Cornelia. Ludwig-Maximilians-Universität. Comparative Tropical Medicine and Parasitology; Alemania. University of Zurich. Institute of Parasitology. National Centre for Vector Entomology; Suiza
Fil: Rudolf, Ivo. The Czech Academy of Sciences. Institute of Vertebrate Biology; República Checa
Fil: Venclíková, Kristýna. The Czech Academy of Sciences. Institute of Vertebrate Biology; República Checa. The Czech Academy of Sciences. Institute of Macromolecular Chemistry; República Checa
Fil: Mahrikova, Lenka. Slovak Academy of Sciences. Institute of Zoology; Eslovaquia
Fil: Slovak, Mirko. Slovak Academy of Sciences. Institute of Zoology; Eslovaquia
Fil: Mendel, Jan. The Czech Academy of Sciences. Institute of Vertebrate Biology; República Checa.
Fil: Blažejová, Hana. The Czech Academy of Sciences. Institute of Vertebrate Biology; República Checa.
Fil: Berthova, Lenka. Slovak Academy of Sciences. Biomedical Research Center. Institute of Virology; Eslovaquia
Fil: Kocianova, Elena. Slovak Academy of Sciences. Biomedical Research Center. Institute of Virology; Eslovaquia
Fil: Hubálek, Zdeněk. The Czech Academy of Sciences. Institute of Vertebrate Biology; República Checa
Fil: Schnittger, Leonhard. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Kazimirová, Mária. Slovak Academy of Sciences. Institute of Zoology; Eslovaquia
description By amplification and sequencing of 18S rRNA gene fragments, Hepatozoon spp. DNA was detected in 0.08 % (4/5057) and 0.04 % (1/2473) of questing Ixodes ricinus ticks from Slovakia and Czech Republic, respectively. Hepatozoon spp. DNA was also detected in spleen and/or lungs of 4.45 % (27/606) of rodents from Slovakia. Prevalence of infection was significantly higher in Myodes glareolus (11.45 %) than in Apodemus spp. (0.28 %) (P < 0.001). Sequencing of 18S rRNA Hepatozoon spp. gene amplicons from I. ricinus showed 100 % identity with Hepatozoon canis isolates from red foxes or dogs in Europe. Phylogenetic analysis showed that at least two H. canis 18S rRNA genotypes exist in Slovakia of which one was identified also in the Czech Republic. The finding of H. canis in questing I. ricinus suggests the geographical spread of the parasite and a potential role of other ticks as its vectors in areas where Rhipicephalus sanguineus is not endemic. Sequencing of 18S rRNA gene amplicons from M. glareolus revealed the presence of two closely related genetic variants, Hepatozoon sp. SK1 and Hepatozoon sp. SK2, showing 99–100 % identity with isolates from M. glareolus from other European countries. Phylogenetic analysis demonstrates that 18S rRNA variants SK1 and SK2 correspond to previously described genotypes UR1 and UR2 of H. erhardovae, respectively. The isolate from Apodemus flavicollis (Hepatozoon sp. SK3b) was 99 % identical with isolates from reptiles in Africa and Asia. Further studies are necessary to identify the taxonomic status of Hepatozoon spp. parasitizing rodents in Europe and the host-parasite interactions in natural foci.
publishDate 2016
dc.date.none.fl_str_mv 2016-10
2017-09-28T13:48:52Z
2017-09-28T13:48:52Z
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/acceptedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str acceptedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/20.500.12123/1347
https://link.springer.com/article/10.1007%2Fs00436-016-5156-5
0932-0113 (Print)
1432-1955 (Online)
https://doi.org/10.1007/s00436-016-5156-5
url http://hdl.handle.net/20.500.12123/1347
https://link.springer.com/article/10.1007%2Fs00436-016-5156-5
https://doi.org/10.1007/s00436-016-5156-5
identifier_str_mv 0932-0113 (Print)
1432-1955 (Online)
dc.language.none.fl_str_mv eng
language eng
dc.rights.none.fl_str_mv info:eu-repo/semantics/restrictedAccess
eu_rights_str_mv restrictedAccess
dc.format.none.fl_str_mv application/pdf
dc.coverage.none.fl_str_mv Slovakia (nation)
Czech Republic (nation)
dc.source.none.fl_str_mv Parasitology research 115 (10) : 3897–3904. (October 2016)
reponame:INTA Digital (INTA)
instname:Instituto Nacional de Tecnología Agropecuaria
reponame_str INTA Digital (INTA)
collection INTA Digital (INTA)
instname_str Instituto Nacional de Tecnología Agropecuaria
repository.name.fl_str_mv INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuaria
repository.mail.fl_str_mv tripaldi.nicolas@inta.gob.ar
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