Borrelia genospecies in Ixodes sp. cf. Ixodes affinis (Acari: Ixodidae) from Argentina

Autores
Flores, Fernando Sebastián; Saracho Bottero, Maria Noelia; Sebastian, Patrick Stephan; Venzal, José Manuel; Mangold, Atilio Jose; Nava, Santiago
Año de publicación
2020
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The aim of this work was to evaluate the presence of Borrelia infection in Ixodes sp. cf. Ixodes affinis ticks from Argentina. Specimens of Ixodes sp. cf. I. affinis were collected on vegetation and birds in five locations belonging the most humid part of the Chaco Biogeographic Province. Specimens were tested for Borrelia infection by nested-PCR targeting the flaB gene and the rrfA-rrlB intergenic spacer region (IGS), sequenced and phylogenetically analyzed. A total of 48 Ixodes sp. cf. I. affinis (12 questing adults from vegetation and 20 nymphs and 16 larvae on nine bird species: Arremon flavirostris, Basileuterus culicivorus, Campylorhamphus trochilirostris, Myiothlypis leucoblephara, Tachyphonus rufus, Thlypopsis sordida, Turdus amaurochalinus, Turdus rufiventris and Troglodytes aedon) were collected. Twelve adults, 14 nymphs and 11 larvae (3 individually and 8 in 3 pools) were analyzed. Partial sequences were detected in 6 adults, 11 nymphs and 4 larvae (2 individual and 2 pools). Phylogenetically, the Borrelia found in Ixodes sp. cf. I. affinis belongs to the B. burgdorferi sensu lato (s. l.) complex. The partial sequences obtained from the borrelian gene flaB and IGS were associated to two groups formed by sequences previously detected in Ixodes fuscipes, Ixodes longiscutatus and Ixodes pararicinus from the Southern Cone of America in northern Argentina, southern Brazil and Uruguay. The results of this work suggest that the haplotypes of B. burgdorferi s. l. complex detected in the three species of the I. ricinus complex distributed in the Southern Cone of America are related and widely distributed.
EEA Rafaela
Fil: Flores, Fernando S. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Ciencias Veterinarias del Litoral. Universidad Nacional del Litoral. Facultad de Ciencias Veterinarias. Instituto de Ciencias Veterinarias del Litoral; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico - CONICET - Santa Fe. Instituto de Ciencias Veterinarias del Litoral. Laboratorio de Ecología de Enfermedades; Argentina
Fil: Saracho Bottero, Maria Noelia. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rafaela; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Sebastian, Patrick S. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rafaela; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Venzal, José Manuel. Universidad de la República. CENUR Litoral Norte-Salto. Facultad de Veterinaria; Uruguay
Fil: Mangold, Atilio Jose. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rafaela; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Nava, Santiago. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rafaela; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fuente
Ticks and Tick-borne Diseases 11 (6) : 101546 (November 2020)
Materia
Ixodes
Ixodes ricinus
Identificación
Argentina
Identification
Garrapatas
Borrelia
Ticks
Nivel de accesibilidad
acceso restringido
Condiciones de uso
Repositorio
INTA Digital (INTA)
Institución
Instituto Nacional de Tecnología Agropecuaria
OAI Identificador
oai:localhost:20.500.12123/7827
Acceder
id INTADig_c994d59d240c40ce50024b519f28b52f
oai_identifier_str oai:localhost:20.500.12123/7827
network_acronym_str INTADig
repository_id_str l
network_name_str INTA Digital (INTA)
spelling Borrelia genospecies in Ixodes sp. cf. Ixodes affinis (Acari: Ixodidae) from ArgentinaFlores, Fernando SebastiánSaracho Bottero, Maria NoeliaSebastian, Patrick StephanVenzal, José ManuelMangold, Atilio JoseNava, SantiagoIxodesIxodes ricinusIdentificaciónArgentinaIdentificationGarrapatasBorreliaTicksThe aim of this work was to evaluate the presence of Borrelia infection in Ixodes sp. cf. Ixodes affinis ticks from Argentina. Specimens of Ixodes sp. cf. I. affinis were collected on vegetation and birds in five locations belonging the most humid part of the Chaco Biogeographic Province. Specimens were tested for Borrelia infection by nested-PCR targeting the flaB gene and the rrfA-rrlB intergenic spacer region (IGS), sequenced and phylogenetically analyzed. A total of 48 Ixodes sp. cf. I. affinis (12 questing adults from vegetation and 20 nymphs and 16 larvae on nine bird species: Arremon flavirostris, Basileuterus culicivorus, Campylorhamphus trochilirostris, Myiothlypis leucoblephara, Tachyphonus rufus, Thlypopsis sordida, Turdus amaurochalinus, Turdus rufiventris and Troglodytes aedon) were collected. Twelve adults, 14 nymphs and 11 larvae (3 individually and 8 in 3 pools) were analyzed. Partial sequences were detected in 6 adults, 11 nymphs and 4 larvae (2 individual and 2 pools). Phylogenetically, the Borrelia found in Ixodes sp. cf. I. affinis belongs to the B. burgdorferi sensu lato (s. l.) complex. The partial sequences obtained from the borrelian gene flaB and IGS were associated to two groups formed by sequences previously detected in Ixodes fuscipes, Ixodes longiscutatus and Ixodes pararicinus from the Southern Cone of America in northern Argentina, southern Brazil and Uruguay. The results of this work suggest that the haplotypes of B. burgdorferi s. l. complex detected in the three species of the I. ricinus complex distributed in the Southern Cone of America are related and widely distributed.EEA RafaelaFil: Flores, Fernando S. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Ciencias Veterinarias del Litoral. Universidad Nacional del Litoral. Facultad de Ciencias Veterinarias. Instituto de Ciencias Veterinarias del Litoral; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico - CONICET - Santa Fe. Instituto de Ciencias Veterinarias del Litoral. Laboratorio de Ecología de Enfermedades; ArgentinaFil: Saracho Bottero, Maria Noelia. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rafaela; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Sebastian, Patrick S. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rafaela; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Venzal, José Manuel. Universidad de la República. CENUR Litoral Norte-Salto. Facultad de Veterinaria; UruguayFil: Mangold, Atilio Jose. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rafaela; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Nava, Santiago. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rafaela; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaElsevier2020-09-04T20:48:58Z2020-09-04T20:48:58Z2020-11info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://hdl.handle.net/20.500.12123/7827https://www.sciencedirect.com/science/article/abs/pii/S1877959X203041551877-959Xhttps://doi.org/10.1016/j.ttbdis.2020.101546Ticks and Tick-borne Diseases 11 (6) : 101546 (November 2020)reponame:INTA Digital (INTA)instname:Instituto Nacional de Tecnología Agropecuariaenginfo:eu-repo/semantics/restrictedAccess2025-09-04T09:48:36Zoai:localhost:20.500.12123/7827instacron:INTAInstitucionalhttp://repositorio.inta.gob.ar/Organismo científico-tecnológicoNo correspondehttp://repositorio.inta.gob.ar/oai/requesttripaldi.nicolas@inta.gob.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:l2025-09-04 09:48:37.282INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuariafalse
dc.title.none.fl_str_mv Borrelia genospecies in Ixodes sp. cf. Ixodes affinis (Acari: Ixodidae) from Argentina
title Borrelia genospecies in Ixodes sp. cf. Ixodes affinis (Acari: Ixodidae) from Argentina
spellingShingle Borrelia genospecies in Ixodes sp. cf. Ixodes affinis (Acari: Ixodidae) from Argentina
Flores, Fernando Sebastián
Ixodes
Ixodes ricinus
Identificación
Argentina
Identification
Garrapatas
Borrelia
Ticks
title_short Borrelia genospecies in Ixodes sp. cf. Ixodes affinis (Acari: Ixodidae) from Argentina
title_full Borrelia genospecies in Ixodes sp. cf. Ixodes affinis (Acari: Ixodidae) from Argentina
title_fullStr Borrelia genospecies in Ixodes sp. cf. Ixodes affinis (Acari: Ixodidae) from Argentina
title_full_unstemmed Borrelia genospecies in Ixodes sp. cf. Ixodes affinis (Acari: Ixodidae) from Argentina
title_sort Borrelia genospecies in Ixodes sp. cf. Ixodes affinis (Acari: Ixodidae) from Argentina
dc.creator.none.fl_str_mv Flores, Fernando Sebastián
Saracho Bottero, Maria Noelia
Sebastian, Patrick Stephan
Venzal, José Manuel
Mangold, Atilio Jose
Nava, Santiago
author Flores, Fernando Sebastián
author_facet Flores, Fernando Sebastián
Saracho Bottero, Maria Noelia
Sebastian, Patrick Stephan
Venzal, José Manuel
Mangold, Atilio Jose
Nava, Santiago
author_role author
author2 Saracho Bottero, Maria Noelia
Sebastian, Patrick Stephan
Venzal, José Manuel
Mangold, Atilio Jose
Nava, Santiago
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv Ixodes
Ixodes ricinus
Identificación
Argentina
Identification
Garrapatas
Borrelia
Ticks
topic Ixodes
Ixodes ricinus
Identificación
Argentina
Identification
Garrapatas
Borrelia
Ticks
dc.description.none.fl_txt_mv The aim of this work was to evaluate the presence of Borrelia infection in Ixodes sp. cf. Ixodes affinis ticks from Argentina. Specimens of Ixodes sp. cf. I. affinis were collected on vegetation and birds in five locations belonging the most humid part of the Chaco Biogeographic Province. Specimens were tested for Borrelia infection by nested-PCR targeting the flaB gene and the rrfA-rrlB intergenic spacer region (IGS), sequenced and phylogenetically analyzed. A total of 48 Ixodes sp. cf. I. affinis (12 questing adults from vegetation and 20 nymphs and 16 larvae on nine bird species: Arremon flavirostris, Basileuterus culicivorus, Campylorhamphus trochilirostris, Myiothlypis leucoblephara, Tachyphonus rufus, Thlypopsis sordida, Turdus amaurochalinus, Turdus rufiventris and Troglodytes aedon) were collected. Twelve adults, 14 nymphs and 11 larvae (3 individually and 8 in 3 pools) were analyzed. Partial sequences were detected in 6 adults, 11 nymphs and 4 larvae (2 individual and 2 pools). Phylogenetically, the Borrelia found in Ixodes sp. cf. I. affinis belongs to the B. burgdorferi sensu lato (s. l.) complex. The partial sequences obtained from the borrelian gene flaB and IGS were associated to two groups formed by sequences previously detected in Ixodes fuscipes, Ixodes longiscutatus and Ixodes pararicinus from the Southern Cone of America in northern Argentina, southern Brazil and Uruguay. The results of this work suggest that the haplotypes of B. burgdorferi s. l. complex detected in the three species of the I. ricinus complex distributed in the Southern Cone of America are related and widely distributed.
EEA Rafaela
Fil: Flores, Fernando S. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Ciencias Veterinarias del Litoral. Universidad Nacional del Litoral. Facultad de Ciencias Veterinarias. Instituto de Ciencias Veterinarias del Litoral; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico - CONICET - Santa Fe. Instituto de Ciencias Veterinarias del Litoral. Laboratorio de Ecología de Enfermedades; Argentina
Fil: Saracho Bottero, Maria Noelia. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rafaela; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Sebastian, Patrick S. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rafaela; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Venzal, José Manuel. Universidad de la República. CENUR Litoral Norte-Salto. Facultad de Veterinaria; Uruguay
Fil: Mangold, Atilio Jose. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rafaela; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Nava, Santiago. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rafaela; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
description The aim of this work was to evaluate the presence of Borrelia infection in Ixodes sp. cf. Ixodes affinis ticks from Argentina. Specimens of Ixodes sp. cf. I. affinis were collected on vegetation and birds in five locations belonging the most humid part of the Chaco Biogeographic Province. Specimens were tested for Borrelia infection by nested-PCR targeting the flaB gene and the rrfA-rrlB intergenic spacer region (IGS), sequenced and phylogenetically analyzed. A total of 48 Ixodes sp. cf. I. affinis (12 questing adults from vegetation and 20 nymphs and 16 larvae on nine bird species: Arremon flavirostris, Basileuterus culicivorus, Campylorhamphus trochilirostris, Myiothlypis leucoblephara, Tachyphonus rufus, Thlypopsis sordida, Turdus amaurochalinus, Turdus rufiventris and Troglodytes aedon) were collected. Twelve adults, 14 nymphs and 11 larvae (3 individually and 8 in 3 pools) were analyzed. Partial sequences were detected in 6 adults, 11 nymphs and 4 larvae (2 individual and 2 pools). Phylogenetically, the Borrelia found in Ixodes sp. cf. I. affinis belongs to the B. burgdorferi sensu lato (s. l.) complex. The partial sequences obtained from the borrelian gene flaB and IGS were associated to two groups formed by sequences previously detected in Ixodes fuscipes, Ixodes longiscutatus and Ixodes pararicinus from the Southern Cone of America in northern Argentina, southern Brazil and Uruguay. The results of this work suggest that the haplotypes of B. burgdorferi s. l. complex detected in the three species of the I. ricinus complex distributed in the Southern Cone of America are related and widely distributed.
publishDate 2020
dc.date.none.fl_str_mv 2020-09-04T20:48:58Z
2020-09-04T20:48:58Z
2020-11
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/20.500.12123/7827
https://www.sciencedirect.com/science/article/abs/pii/S1877959X20304155
1877-959X
https://doi.org/10.1016/j.ttbdis.2020.101546
url http://hdl.handle.net/20.500.12123/7827
https://www.sciencedirect.com/science/article/abs/pii/S1877959X20304155
https://doi.org/10.1016/j.ttbdis.2020.101546
identifier_str_mv 1877-959X
dc.language.none.fl_str_mv eng
language eng
dc.rights.none.fl_str_mv info:eu-repo/semantics/restrictedAccess
eu_rights_str_mv restrictedAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv Ticks and Tick-borne Diseases 11 (6) : 101546 (November 2020)
reponame:INTA Digital (INTA)
instname:Instituto Nacional de Tecnología Agropecuaria
reponame_str INTA Digital (INTA)
collection INTA Digital (INTA)
instname_str Instituto Nacional de Tecnología Agropecuaria
repository.name.fl_str_mv INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuaria
repository.mail.fl_str_mv tripaldi.nicolas@inta.gob.ar
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score 12.623145

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