Prevalence and viability of group A rotavirus in dairy farm water sources
- Autores
- Castells, M.L.; Schild, Carlos Omar; Caffarena, Rubén Dario; Bok, Marina; Giannitti, Federico; Armendano, Joaquín Ignacio; Riet‐Correa, Franklin; Victoria, M.; Parreño, Viviana; Colina, Rodney
- Año de publicación
- 2018
- Idioma
- inglés
- Tipo de recurso
- artículo
- Estado
- versión publicada
- Descripción
- Aim: To analyse group A rotavirus (RVA) environmental contamination in waters used for calves' consumption and to assess viral viability in dairy farm water sources. Methods and Results: We analysed 202 samples of water used for calves' consumption and RVA was detected by RT‐qPCR in 35·1% (95% CI: 28·9–42·0%). A marked pattern of seasonality was observed with higher frequency of detection in colder than warmer months (P = 0·002). There was no association between viral load and season or between the number of milking cows in the herd and the detection of RVA in the farm. The viability of the RVA particles detected was confirmed by isolation of RVA in cell culture from 5 of 10 water samples. Furthermore, an RVA waterborne outbreak of neonatal calf diarrhoea was described. Conclusions: We demonstrate that RVA is frequent in dairy farm waters, and that the virus is infectious and capable of generating a diarrhoea outbreak. Significance and Impact of the Study: Neonatal diarrhoea syndrome leads to economic losses to the livestock industry worldwide. To determine transmission routes is essential to take action in this regard and reduce the impact that this syndrome has for the livestock production. The results obtained in this work alert the dairy industry and highlight that mitigation strategies are crucial to improve the microbiological quality of this water.
Instituto de Virología
Fil: Castells, M.L. Universidad de la República. CENUR Litoral Norte. Centro Universitario de Salto. Laboratorio de Virología Molecular; Uruguay
Fil: Schild, Carlos Omar. Instituto Nacional de Investigación Agropecuaria (INIA), La Estanzuela. Plataforma de Investigación en Salud Animal; Uruguay
Fil: Caffarena, Rubén Dario. Instituto Nacional de Investigación Agropecuaria (INIA), La Estanzuela. Plataforma de Investigación en Salud Animal; Uruguay
Fil: Bok, Marina. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina
Fil: Giannitti, Federico. Instituto Nacional de Investigación Agropecuaria (INIA), La Estanzuela. Plataforma de Investigación en Salud Animal; Uruguay. University of Minnesota. College of Veterinary Medicine. Veterinary Population Medicine Department; Estados Unidos
Fil: Armendano, Joaquín Ignacio. Universidad Nacional de Mar del Plata. Facultad de Ciencias Agrarias; Argentina
Fil: Riet‐Correa, Franklin. Instituto Nacional de Investigación Agropecuaria (INIA), La Estanzuela. Plataforma de Investigación en Salud Animal; Uruguay
Fil: Victoria, M. Universidad de la República. CENUR Litoral Norte. Centro Universitario de Salto. Laboratorio de Virología Molecular; Uruguay
Fil: Parreño, Viviana. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina
Fil: Colina, Rodney. Universidad de la República. CENUR Litoral Norte. Centro Universitario de Salto. Laboratorio de Virología Molecular; Uruguay - Fuente
- Journal of applied microbiology 124 (3) : 922-929. (March 2018)
- Materia
-
Rotavirus
Granjas Lecheras
Enfermedades de los Animales
Morbosidad
Agua
Dairy Farms
Animal Diseases
Morbidity
Water
Rotavirus A
Prevalencia de una enfermedad
Fuentes de Agua - Nivel de accesibilidad
- acceso restringido
- Condiciones de uso
- Repositorio
.jpg)
- Institución
- Instituto Nacional de Tecnología Agropecuaria
- OAI Identificador
- oai:localhost:20.500.12123/2230
Ver los metadatos del registro completo
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Prevalence and viability of group A rotavirus in dairy farm water sourcesCastells, M.L.Schild, Carlos OmarCaffarena, Rubén DarioBok, MarinaGiannitti, FedericoArmendano, Joaquín IgnacioRiet‐Correa, FranklinVictoria, M.Parreño, VivianaColina, RodneyRotavirusGranjas LecherasEnfermedades de los AnimalesMorbosidadAguaDairy FarmsAnimal DiseasesMorbidityWaterRotavirus APrevalencia de una enfermedadFuentes de AguaAim: To analyse group A rotavirus (RVA) environmental contamination in waters used for calves' consumption and to assess viral viability in dairy farm water sources. Methods and Results: We analysed 202 samples of water used for calves' consumption and RVA was detected by RT‐qPCR in 35·1% (95% CI: 28·9–42·0%). A marked pattern of seasonality was observed with higher frequency of detection in colder than warmer months (P = 0·002). There was no association between viral load and season or between the number of milking cows in the herd and the detection of RVA in the farm. The viability of the RVA particles detected was confirmed by isolation of RVA in cell culture from 5 of 10 water samples. Furthermore, an RVA waterborne outbreak of neonatal calf diarrhoea was described. Conclusions: We demonstrate that RVA is frequent in dairy farm waters, and that the virus is infectious and capable of generating a diarrhoea outbreak. Significance and Impact of the Study: Neonatal diarrhoea syndrome leads to economic losses to the livestock industry worldwide. To determine transmission routes is essential to take action in this regard and reduce the impact that this syndrome has for the livestock production. The results obtained in this work alert the dairy industry and highlight that mitigation strategies are crucial to improve the microbiological quality of this water.Instituto de VirologíaFil: Castells, M.L. Universidad de la República. CENUR Litoral Norte. Centro Universitario de Salto. Laboratorio de Virología Molecular; UruguayFil: Schild, Carlos Omar. Instituto Nacional de Investigación Agropecuaria (INIA), La Estanzuela. Plataforma de Investigación en Salud Animal; UruguayFil: Caffarena, Rubén Dario. Instituto Nacional de Investigación Agropecuaria (INIA), La Estanzuela. Plataforma de Investigación en Salud Animal; UruguayFil: Bok, Marina. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; ArgentinaFil: Giannitti, Federico. Instituto Nacional de Investigación Agropecuaria (INIA), La Estanzuela. Plataforma de Investigación en Salud Animal; Uruguay. University of Minnesota. College of Veterinary Medicine. Veterinary Population Medicine Department; Estados UnidosFil: Armendano, Joaquín Ignacio. Universidad Nacional de Mar del Plata. Facultad de Ciencias Agrarias; ArgentinaFil: Riet‐Correa, Franklin. Instituto Nacional de Investigación Agropecuaria (INIA), La Estanzuela. Plataforma de Investigación en Salud Animal; UruguayFil: Victoria, M. Universidad de la República. CENUR Litoral Norte. Centro Universitario de Salto. Laboratorio de Virología Molecular; UruguayFil: Parreño, Viviana. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; ArgentinaFil: Colina, Rodney. Universidad de la República. CENUR Litoral Norte. Centro Universitario de Salto. Laboratorio de Virología Molecular; Uruguay2018-04-12T14:49:09Z2018-04-12T14:49:09Z2018-03info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttps://onlinelibrary.wiley.com/doi/abs/10.1111/jam.13691http://hdl.handle.net/20.500.12123/22301364-50721365-2672https://doi.org/10.1111/jam.13691Journal of applied microbiology 124 (3) : 922-929. (March 2018)reponame:INTA Digital (INTA)instname:Instituto Nacional de Tecnología Agropecuariaenginfo:eu-repo/semantics/restrictedAccess2025-09-11T10:22:21Zoai:localhost:20.500.12123/2230instacron:INTAInstitucionalhttp://repositorio.inta.gob.ar/Organismo científico-tecnológicoNo correspondehttp://repositorio.inta.gob.ar/oai/requesttripaldi.nicolas@inta.gob.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:l2025-09-11 10:22:22.157INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuariafalse |
| dc.title.none.fl_str_mv |
Prevalence and viability of group A rotavirus in dairy farm water sources |
| title |
Prevalence and viability of group A rotavirus in dairy farm water sources |
| spellingShingle |
Prevalence and viability of group A rotavirus in dairy farm water sources Castells, M.L. Rotavirus Granjas Lecheras Enfermedades de los Animales Morbosidad Agua Dairy Farms Animal Diseases Morbidity Water Rotavirus A Prevalencia de una enfermedad Fuentes de Agua |
| title_short |
Prevalence and viability of group A rotavirus in dairy farm water sources |
| title_full |
Prevalence and viability of group A rotavirus in dairy farm water sources |
| title_fullStr |
Prevalence and viability of group A rotavirus in dairy farm water sources |
| title_full_unstemmed |
Prevalence and viability of group A rotavirus in dairy farm water sources |
| title_sort |
Prevalence and viability of group A rotavirus in dairy farm water sources |
| dc.creator.none.fl_str_mv |
Castells, M.L. Schild, Carlos Omar Caffarena, Rubén Dario Bok, Marina Giannitti, Federico Armendano, Joaquín Ignacio Riet‐Correa, Franklin Victoria, M. Parreño, Viviana Colina, Rodney |
| author |
Castells, M.L. |
| author_facet |
Castells, M.L. Schild, Carlos Omar Caffarena, Rubén Dario Bok, Marina Giannitti, Federico Armendano, Joaquín Ignacio Riet‐Correa, Franklin Victoria, M. Parreño, Viviana Colina, Rodney |
| author_role |
author |
| author2 |
Schild, Carlos Omar Caffarena, Rubén Dario Bok, Marina Giannitti, Federico Armendano, Joaquín Ignacio Riet‐Correa, Franklin Victoria, M. Parreño, Viviana Colina, Rodney |
| author2_role |
author author author author author author author author author |
| dc.subject.none.fl_str_mv |
Rotavirus Granjas Lecheras Enfermedades de los Animales Morbosidad Agua Dairy Farms Animal Diseases Morbidity Water Rotavirus A Prevalencia de una enfermedad Fuentes de Agua |
| topic |
Rotavirus Granjas Lecheras Enfermedades de los Animales Morbosidad Agua Dairy Farms Animal Diseases Morbidity Water Rotavirus A Prevalencia de una enfermedad Fuentes de Agua |
| dc.description.none.fl_txt_mv |
Aim: To analyse group A rotavirus (RVA) environmental contamination in waters used for calves' consumption and to assess viral viability in dairy farm water sources. Methods and Results: We analysed 202 samples of water used for calves' consumption and RVA was detected by RT‐qPCR in 35·1% (95% CI: 28·9–42·0%). A marked pattern of seasonality was observed with higher frequency of detection in colder than warmer months (P = 0·002). There was no association between viral load and season or between the number of milking cows in the herd and the detection of RVA in the farm. The viability of the RVA particles detected was confirmed by isolation of RVA in cell culture from 5 of 10 water samples. Furthermore, an RVA waterborne outbreak of neonatal calf diarrhoea was described. Conclusions: We demonstrate that RVA is frequent in dairy farm waters, and that the virus is infectious and capable of generating a diarrhoea outbreak. Significance and Impact of the Study: Neonatal diarrhoea syndrome leads to economic losses to the livestock industry worldwide. To determine transmission routes is essential to take action in this regard and reduce the impact that this syndrome has for the livestock production. The results obtained in this work alert the dairy industry and highlight that mitigation strategies are crucial to improve the microbiological quality of this water. Instituto de Virología Fil: Castells, M.L. Universidad de la República. CENUR Litoral Norte. Centro Universitario de Salto. Laboratorio de Virología Molecular; Uruguay Fil: Schild, Carlos Omar. Instituto Nacional de Investigación Agropecuaria (INIA), La Estanzuela. Plataforma de Investigación en Salud Animal; Uruguay Fil: Caffarena, Rubén Dario. Instituto Nacional de Investigación Agropecuaria (INIA), La Estanzuela. Plataforma de Investigación en Salud Animal; Uruguay Fil: Bok, Marina. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina Fil: Giannitti, Federico. Instituto Nacional de Investigación Agropecuaria (INIA), La Estanzuela. Plataforma de Investigación en Salud Animal; Uruguay. University of Minnesota. College of Veterinary Medicine. Veterinary Population Medicine Department; Estados Unidos Fil: Armendano, Joaquín Ignacio. Universidad Nacional de Mar del Plata. Facultad de Ciencias Agrarias; Argentina Fil: Riet‐Correa, Franklin. Instituto Nacional de Investigación Agropecuaria (INIA), La Estanzuela. Plataforma de Investigación en Salud Animal; Uruguay Fil: Victoria, M. Universidad de la República. CENUR Litoral Norte. Centro Universitario de Salto. Laboratorio de Virología Molecular; Uruguay Fil: Parreño, Viviana. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina Fil: Colina, Rodney. Universidad de la República. CENUR Litoral Norte. Centro Universitario de Salto. Laboratorio de Virología Molecular; Uruguay |
| description |
Aim: To analyse group A rotavirus (RVA) environmental contamination in waters used for calves' consumption and to assess viral viability in dairy farm water sources. Methods and Results: We analysed 202 samples of water used for calves' consumption and RVA was detected by RT‐qPCR in 35·1% (95% CI: 28·9–42·0%). A marked pattern of seasonality was observed with higher frequency of detection in colder than warmer months (P = 0·002). There was no association between viral load and season or between the number of milking cows in the herd and the detection of RVA in the farm. The viability of the RVA particles detected was confirmed by isolation of RVA in cell culture from 5 of 10 water samples. Furthermore, an RVA waterborne outbreak of neonatal calf diarrhoea was described. Conclusions: We demonstrate that RVA is frequent in dairy farm waters, and that the virus is infectious and capable of generating a diarrhoea outbreak. Significance and Impact of the Study: Neonatal diarrhoea syndrome leads to economic losses to the livestock industry worldwide. To determine transmission routes is essential to take action in this regard and reduce the impact that this syndrome has for the livestock production. The results obtained in this work alert the dairy industry and highlight that mitigation strategies are crucial to improve the microbiological quality of this water. |
| publishDate |
2018 |
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2018-04-12T14:49:09Z 2018-04-12T14:49:09Z 2018-03 |
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info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://purl.org/coar/resource_type/c_6501 info:ar-repo/semantics/articulo |
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article |
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publishedVersion |
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https://onlinelibrary.wiley.com/doi/abs/10.1111/jam.13691 http://hdl.handle.net/20.500.12123/2230 1364-5072 1365-2672 https://doi.org/10.1111/jam.13691 |
| url |
https://onlinelibrary.wiley.com/doi/abs/10.1111/jam.13691 http://hdl.handle.net/20.500.12123/2230 https://doi.org/10.1111/jam.13691 |
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1364-5072 1365-2672 |
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eng |
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