Ovine abortion by Neospora caninum: first case reported in Argentina
- Autores
- Hecker, Yanina; Morrell, Eleonora Lidia; Fiorentino, María Andrea; Gual, Ignacio; Rivera, Emilia; Fiorani, Franco; Dorsch, Matías; Gos, María Laura; Pardini, Lais; Scioli, María; Magariños, Sergio; Paolicchi, Fernando; Canton, German Jose; Moore, Prando Dadin
- Año de publicación
- 2019
- Idioma
- inglés
- Tipo de recurso
- artículo
- Estado
- versión publicada
- Descripción
- Purpose The aim of this study was to describe for the first time a natural case of ovine abortion associated with Neospora caninum in a flock with reproductive losses in Argentina. Materials and Methods The analyzed flock consisted of 256 Texel sheep, of which 134 had been mated. A single blood sample was obtained by jugular vein puncture from 220 ewes (116 adult ewes, 104 yearling ewes) and 93 lambs. Serum samples and fetal fluid were tested using the indirect fluorescence antibody test to detect antibodies against N. caninum and T. gondii. Fetal and placental tissues from aborted fetus were examined by standard gross pathology procedures and were tested using histopathology and immunohistochemistry. Moreover, DNA from fetal and placental tissues was isolated and a PCR assay to detect N. caninum, T. gondii and Chlamydia spp. was used. Results and Discussion The pregnancy rate was 89% (119/134), the abortion rate was 8.4% (10/119) whereas the perinatal mortality rate was 15% (16/109). Out of 116 adult ewes sampled, 34.48% presented anti-N. caninum antibodies. Ten ewes had aborted, and one fetus was directly submitted to the diagnostic laboratory for further processing. Antibodies against N. caninum were detected in fetal fluid and in the aborted dam. Histopathological analysis in fetal tissues showed multifocal lymphohistiocytic glossitis, diffuse mild lymphohistiocytic endocarditis, pericarditis and focally extensive myocarditis. Severe multifocal necrotizing placentitis and diffuse mild lymphohistiocytic placentitis with the presence of lymphohistiocytic vasculitis were observed in placenta. N. caninum was immunolabeled in the placenta and fetal tongue. In addition, N. caninum DNA was detected in placenta, central neural system, lung and heart of the aborted fetus. There was no evidence of other infectious abortifacients in the aborted fetus. Conclusion The present study described for the first time an ovine abortion caused by N. caninum in Argentina. Further investigations at a larger scale are required to establish the role of N. caninum as an important cause of reproductive losses in sheep flocks from the region.
EEA Balcarce
Fil: Hecker, Yanina Paola. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET); Argentina.
Fil: Morrel, Eleonora Lidia. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina.
Fil: Fiorentino, María Andrea. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina.
Fil: Gual, Ignacio. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina. Universidad Nacional de Mar del Plata. Facultad de Ciencias Agrarias; Argentina.
Fil: Rivera, Emilia. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina.
Fil: Fiorani, Franco. Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET); Argentina. Universidad Nacional de Mar del Plata. Facultad de Ciencias Agrarias; Argentina.
Fil: Dorsch, Matías A. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina. Universidad Nacional de Mar del Plata. Facultad de Ciencias Agrarias; Argentina.
Fil; Gos, María L. Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET); Argentina. Universidad Nacional de La Plata. Facultad de Medicina Veterinaria; Argentina.
Fil: Pardini, Lais L. Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET); Argentina. Universidad Nacional de La Plata. Facultad de Medicina Veterinaria; Argentina.
Fil: Scioli, María V. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina.
Fil: Magariños, Sergio. Actividad privada; Argentina.
Fil: Paolicchi, Fernando A. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina. Universidad Nacional de Mar del Plata. Facultad de Ciencias Agrarias; Argentina.
Fil: Cantón, Germán José. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina.
Fil: Moore, Dadín Prando. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina. Universidad Nacional de Mar del Plata. Facultad de Ciencias Agrarias; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET); Argentina. - Fuente
- Acta parasitologica : 1-6. (04 September 2019)
- Materia
-
Ovino
Aborto
Neospora Caninum
Argentina
Sheep
Abortion
Reproductive Health
Salud Reproductiva
Reproductive Losses - Nivel de accesibilidad
- acceso restringido
- Condiciones de uso
- Repositorio
- Institución
- Instituto Nacional de Tecnología Agropecuaria
- OAI Identificador
- oai:localhost:20.500.12123/6036
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Ovine abortion by Neospora caninum: first case reported in ArgentinaHecker, YaninaMorrell, Eleonora LidiaFiorentino, María AndreaGual, IgnacioRivera, EmiliaFiorani, FrancoDorsch, MatíasGos, María LauraPardini, LaisScioli, MaríaMagariños, SergioPaolicchi, FernandoCanton, German JoseMoore, Prando DadinOvinoAbortoNeospora CaninumArgentinaSheepAbortionReproductive HealthSalud ReproductivaReproductive LossesPurpose The aim of this study was to describe for the first time a natural case of ovine abortion associated with Neospora caninum in a flock with reproductive losses in Argentina. Materials and Methods The analyzed flock consisted of 256 Texel sheep, of which 134 had been mated. A single blood sample was obtained by jugular vein puncture from 220 ewes (116 adult ewes, 104 yearling ewes) and 93 lambs. Serum samples and fetal fluid were tested using the indirect fluorescence antibody test to detect antibodies against N. caninum and T. gondii. Fetal and placental tissues from aborted fetus were examined by standard gross pathology procedures and were tested using histopathology and immunohistochemistry. Moreover, DNA from fetal and placental tissues was isolated and a PCR assay to detect N. caninum, T. gondii and Chlamydia spp. was used. Results and Discussion The pregnancy rate was 89% (119/134), the abortion rate was 8.4% (10/119) whereas the perinatal mortality rate was 15% (16/109). Out of 116 adult ewes sampled, 34.48% presented anti-N. caninum antibodies. Ten ewes had aborted, and one fetus was directly submitted to the diagnostic laboratory for further processing. Antibodies against N. caninum were detected in fetal fluid and in the aborted dam. Histopathological analysis in fetal tissues showed multifocal lymphohistiocytic glossitis, diffuse mild lymphohistiocytic endocarditis, pericarditis and focally extensive myocarditis. Severe multifocal necrotizing placentitis and diffuse mild lymphohistiocytic placentitis with the presence of lymphohistiocytic vasculitis were observed in placenta. N. caninum was immunolabeled in the placenta and fetal tongue. In addition, N. caninum DNA was detected in placenta, central neural system, lung and heart of the aborted fetus. There was no evidence of other infectious abortifacients in the aborted fetus. Conclusion The present study described for the first time an ovine abortion caused by N. caninum in Argentina. Further investigations at a larger scale are required to establish the role of N. caninum as an important cause of reproductive losses in sheep flocks from the region.EEA BalcarceFil: Hecker, Yanina Paola. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET); Argentina.Fil: Morrel, Eleonora Lidia. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina.Fil: Fiorentino, María Andrea. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina.Fil: Gual, Ignacio. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina. Universidad Nacional de Mar del Plata. Facultad de Ciencias Agrarias; Argentina.Fil: Rivera, Emilia. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina.Fil: Fiorani, Franco. Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET); Argentina. Universidad Nacional de Mar del Plata. Facultad de Ciencias Agrarias; Argentina.Fil: Dorsch, Matías A. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina. Universidad Nacional de Mar del Plata. Facultad de Ciencias Agrarias; Argentina.Fil; Gos, María L. Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET); Argentina. Universidad Nacional de La Plata. Facultad de Medicina Veterinaria; Argentina.Fil: Pardini, Lais L. Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET); Argentina. Universidad Nacional de La Plata. Facultad de Medicina Veterinaria; Argentina.Fil: Scioli, María V. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina.Fil: Magariños, Sergio. Actividad privada; Argentina.Fil: Paolicchi, Fernando A. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina. Universidad Nacional de Mar del Plata. Facultad de Ciencias Agrarias; Argentina.Fil: Cantón, Germán José. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina.Fil: Moore, Dadín Prando. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina. Universidad Nacional de Mar del Plata. Facultad de Ciencias Agrarias; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET); Argentina.Springer Verlag Germany2019-10-02T14:35:59Z2019-10-02T14:35:59Z2019-09-04info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttps://link.springer.com/article/10.2478/s11686-019-00106-zhttp://hdl.handle.net/20.500.12123/60361230-28211896-1851 (Online)https://doi.org/10.2478/s11686-019-00106-zActa parasitologica : 1-6. 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dc.title.none.fl_str_mv |
Ovine abortion by Neospora caninum: first case reported in Argentina |
title |
Ovine abortion by Neospora caninum: first case reported in Argentina |
spellingShingle |
Ovine abortion by Neospora caninum: first case reported in Argentina Hecker, Yanina Ovino Aborto Neospora Caninum Argentina Sheep Abortion Reproductive Health Salud Reproductiva Reproductive Losses |
title_short |
Ovine abortion by Neospora caninum: first case reported in Argentina |
title_full |
Ovine abortion by Neospora caninum: first case reported in Argentina |
title_fullStr |
Ovine abortion by Neospora caninum: first case reported in Argentina |
title_full_unstemmed |
Ovine abortion by Neospora caninum: first case reported in Argentina |
title_sort |
Ovine abortion by Neospora caninum: first case reported in Argentina |
dc.creator.none.fl_str_mv |
Hecker, Yanina Morrell, Eleonora Lidia Fiorentino, María Andrea Gual, Ignacio Rivera, Emilia Fiorani, Franco Dorsch, Matías Gos, María Laura Pardini, Lais Scioli, María Magariños, Sergio Paolicchi, Fernando Canton, German Jose Moore, Prando Dadin |
author |
Hecker, Yanina |
author_facet |
Hecker, Yanina Morrell, Eleonora Lidia Fiorentino, María Andrea Gual, Ignacio Rivera, Emilia Fiorani, Franco Dorsch, Matías Gos, María Laura Pardini, Lais Scioli, María Magariños, Sergio Paolicchi, Fernando Canton, German Jose Moore, Prando Dadin |
author_role |
author |
author2 |
Morrell, Eleonora Lidia Fiorentino, María Andrea Gual, Ignacio Rivera, Emilia Fiorani, Franco Dorsch, Matías Gos, María Laura Pardini, Lais Scioli, María Magariños, Sergio Paolicchi, Fernando Canton, German Jose Moore, Prando Dadin |
author2_role |
author author author author author author author author author author author author author |
dc.subject.none.fl_str_mv |
Ovino Aborto Neospora Caninum Argentina Sheep Abortion Reproductive Health Salud Reproductiva Reproductive Losses |
topic |
Ovino Aborto Neospora Caninum Argentina Sheep Abortion Reproductive Health Salud Reproductiva Reproductive Losses |
dc.description.none.fl_txt_mv |
Purpose The aim of this study was to describe for the first time a natural case of ovine abortion associated with Neospora caninum in a flock with reproductive losses in Argentina. Materials and Methods The analyzed flock consisted of 256 Texel sheep, of which 134 had been mated. A single blood sample was obtained by jugular vein puncture from 220 ewes (116 adult ewes, 104 yearling ewes) and 93 lambs. Serum samples and fetal fluid were tested using the indirect fluorescence antibody test to detect antibodies against N. caninum and T. gondii. Fetal and placental tissues from aborted fetus were examined by standard gross pathology procedures and were tested using histopathology and immunohistochemistry. Moreover, DNA from fetal and placental tissues was isolated and a PCR assay to detect N. caninum, T. gondii and Chlamydia spp. was used. Results and Discussion The pregnancy rate was 89% (119/134), the abortion rate was 8.4% (10/119) whereas the perinatal mortality rate was 15% (16/109). Out of 116 adult ewes sampled, 34.48% presented anti-N. caninum antibodies. Ten ewes had aborted, and one fetus was directly submitted to the diagnostic laboratory for further processing. Antibodies against N. caninum were detected in fetal fluid and in the aborted dam. Histopathological analysis in fetal tissues showed multifocal lymphohistiocytic glossitis, diffuse mild lymphohistiocytic endocarditis, pericarditis and focally extensive myocarditis. Severe multifocal necrotizing placentitis and diffuse mild lymphohistiocytic placentitis with the presence of lymphohistiocytic vasculitis were observed in placenta. N. caninum was immunolabeled in the placenta and fetal tongue. In addition, N. caninum DNA was detected in placenta, central neural system, lung and heart of the aborted fetus. There was no evidence of other infectious abortifacients in the aborted fetus. Conclusion The present study described for the first time an ovine abortion caused by N. caninum in Argentina. Further investigations at a larger scale are required to establish the role of N. caninum as an important cause of reproductive losses in sheep flocks from the region. EEA Balcarce Fil: Hecker, Yanina Paola. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET); Argentina. Fil: Morrel, Eleonora Lidia. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina. Fil: Fiorentino, María Andrea. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina. Fil: Gual, Ignacio. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina. Universidad Nacional de Mar del Plata. Facultad de Ciencias Agrarias; Argentina. Fil: Rivera, Emilia. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina. Fil: Fiorani, Franco. Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET); Argentina. Universidad Nacional de Mar del Plata. Facultad de Ciencias Agrarias; Argentina. Fil: Dorsch, Matías A. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina. Universidad Nacional de Mar del Plata. Facultad de Ciencias Agrarias; Argentina. Fil; Gos, María L. Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET); Argentina. Universidad Nacional de La Plata. Facultad de Medicina Veterinaria; Argentina. Fil: Pardini, Lais L. Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET); Argentina. Universidad Nacional de La Plata. Facultad de Medicina Veterinaria; Argentina. Fil: Scioli, María V. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina. Fil: Magariños, Sergio. Actividad privada; Argentina. Fil: Paolicchi, Fernando A. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina. Universidad Nacional de Mar del Plata. Facultad de Ciencias Agrarias; Argentina. Fil: Cantón, Germán José. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina. Fil: Moore, Dadín Prando. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina. Universidad Nacional de Mar del Plata. Facultad de Ciencias Agrarias; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET); Argentina. |
description |
Purpose The aim of this study was to describe for the first time a natural case of ovine abortion associated with Neospora caninum in a flock with reproductive losses in Argentina. Materials and Methods The analyzed flock consisted of 256 Texel sheep, of which 134 had been mated. A single blood sample was obtained by jugular vein puncture from 220 ewes (116 adult ewes, 104 yearling ewes) and 93 lambs. Serum samples and fetal fluid were tested using the indirect fluorescence antibody test to detect antibodies against N. caninum and T. gondii. Fetal and placental tissues from aborted fetus were examined by standard gross pathology procedures and were tested using histopathology and immunohistochemistry. Moreover, DNA from fetal and placental tissues was isolated and a PCR assay to detect N. caninum, T. gondii and Chlamydia spp. was used. Results and Discussion The pregnancy rate was 89% (119/134), the abortion rate was 8.4% (10/119) whereas the perinatal mortality rate was 15% (16/109). Out of 116 adult ewes sampled, 34.48% presented anti-N. caninum antibodies. Ten ewes had aborted, and one fetus was directly submitted to the diagnostic laboratory for further processing. Antibodies against N. caninum were detected in fetal fluid and in the aborted dam. Histopathological analysis in fetal tissues showed multifocal lymphohistiocytic glossitis, diffuse mild lymphohistiocytic endocarditis, pericarditis and focally extensive myocarditis. Severe multifocal necrotizing placentitis and diffuse mild lymphohistiocytic placentitis with the presence of lymphohistiocytic vasculitis were observed in placenta. N. caninum was immunolabeled in the placenta and fetal tongue. In addition, N. caninum DNA was detected in placenta, central neural system, lung and heart of the aborted fetus. There was no evidence of other infectious abortifacients in the aborted fetus. Conclusion The present study described for the first time an ovine abortion caused by N. caninum in Argentina. Further investigations at a larger scale are required to establish the role of N. caninum as an important cause of reproductive losses in sheep flocks from the region. |
publishDate |
2019 |
dc.date.none.fl_str_mv |
2019-10-02T14:35:59Z 2019-10-02T14:35:59Z 2019-09-04 |
dc.type.none.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://purl.org/coar/resource_type/c_6501 info:ar-repo/semantics/articulo |
format |
article |
status_str |
publishedVersion |
dc.identifier.none.fl_str_mv |
https://link.springer.com/article/10.2478/s11686-019-00106-z http://hdl.handle.net/20.500.12123/6036 1230-2821 1896-1851 (Online) https://doi.org/10.2478/s11686-019-00106-z |
url |
https://link.springer.com/article/10.2478/s11686-019-00106-z http://hdl.handle.net/20.500.12123/6036 https://doi.org/10.2478/s11686-019-00106-z |
identifier_str_mv |
1230-2821 1896-1851 (Online) |
dc.language.none.fl_str_mv |
eng |
language |
eng |
dc.rights.none.fl_str_mv |
info:eu-repo/semantics/restrictedAccess |
eu_rights_str_mv |
restrictedAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Springer Verlag Germany |
publisher.none.fl_str_mv |
Springer Verlag Germany |
dc.source.none.fl_str_mv |
Acta parasitologica : 1-6. (04 September 2019) reponame:INTA Digital (INTA) instname:Instituto Nacional de Tecnología Agropecuaria |
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INTA Digital (INTA) |
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INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuaria |
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tripaldi.nicolas@inta.gob.ar |
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