Fungicide resistance in Cercospora species causing cercospora leaf blight and purple seed stain of soybean in Argentina

Autores
Sautua, Francisco José; Doyle, Vinson P.; Price, Paul P.; Porfiri, Alejandro; Fernandez, Paula Del Carmen; Scandiani, María Mercedes; Carmona, Marcelo Anibal
Año de publicación
2020
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Cercospora species cause cercospora leaf blight (CLB) and purple seed stain (PSS) on soybean. Because there are few resistant soybean varieties available, CLB/PSS management relies heavily upon fungicide applications. Sensitivity of 62 Argentinian Cercospora isolates to demethylation inhibitor (DMI), methyl benzimidazole carbamate (MBC), quinone outside inhibitor (QoI), succinate dehydrogenase inhibitor (SDHI) fungicides, and mancozeb was determined in this study. All isolates were sensitive to difenoconazole, epoxiconazole, prothioconazole, tebuconazole, and cyproconazole (EC50 values ranged from 0.006 to 2.4 µg/ml). In contrast, 51% of the tested isolates were sensitive (EC50 values ranged from 0.003 to 0.2 µg/ml), and 49% were highly resistant (EC50 > 100 µg/ml) to carbendazim. Interestingly, all isolates were completely resistant to azoxystrobin, trifloxystrobin, and pyraclostrobin, and insensitive to boscalid, fluxapyroxad, and pydiflumetofen (EC50 > 100 µg/ml). The G143A mutation was detected in 82% (53) of the QoI-resistant isolates and the E198A mutation in 97% (31) of the carbendazim-resistant isolates. No apparent resistance mutations were detected in the succinate dehydrogenase genes (subunits sdhB, sdhC, and sdhD). Mancozeb completely inhibited mycelial growth of the isolates evaluated at a concentration of 100 µg/ml. All Argentinian Cercospora isolates were sensitive to the DMI fungicides tested, but we report for the first time resistance to QoI and MBC fungicides. Mechanism(s) other than fungicide target-site modification may be responsible for resistance of Cercospora to QoI and MBC fungicides. Moreover, based on our results and on the recent introduction of SDHI fungicides on soybean in Argentina, Cercospora species causing CLB/PSS are insensitive (naturally resistant) to SDHI fungicides. Insensitivity must be confirmed under field conditions.
Instituto de Biotecnología
Fil: Sautua, Francisco José. Universidad de Buenos Aires. Facultad de Agronomía. Cátedra de Fitopatología; Argentina
Fil: Doyle, Vinson P. LSU AgCenter. Department of Plant Pathology and Crop Physiology; Estados Unidos
Fil: Price, Paul P. LSU AgCenter. Macon Ridge Research Station; Estados Unidos
Fil: Porfiri, Alejandro. Consultor independiente; Argentina
Fil: Fernandez, Paula Del Carmen. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina.
Fil: Fernandez, Paula Del Carmen. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Scandiani, María Mercedes. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Centro de Referencia de Micología (CEREMIC); Argentina
Fil: Carmona, Marcelo Anibal. Universidad de Buenos Aires. Facultad de Agronomía. Cátedra de Fitopatología; Argentina
Fuente
Plant Pathology 69 (9) : 1678-1694 (December 2020)
Materia
Cercospora
Fungicidas
Resistencia a los Fungicidas
Soja
Enfermedades de las Plantas
Fungicides
Resistance to Fungicides
Soybeans
Plant Diseases
Argentina
Nivel de accesibilidad
acceso restringido
Condiciones de uso
Repositorio
INTA Digital (INTA)
Institución
Instituto Nacional de Tecnología Agropecuaria
OAI Identificador
oai:localhost:20.500.12123/11121
Acceder
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oai_identifier_str oai:localhost:20.500.12123/11121
network_acronym_str INTADig
repository_id_str l
network_name_str INTA Digital (INTA)
spelling Fungicide resistance in Cercospora species causing cercospora leaf blight and purple seed stain of soybean in ArgentinaSautua, Francisco JoséDoyle, Vinson P.Price, Paul P.Porfiri, AlejandroFernandez, Paula Del CarmenScandiani, María MercedesCarmona, Marcelo AnibalCercosporaFungicidasResistencia a los FungicidasSojaEnfermedades de las PlantasFungicidesResistance to FungicidesSoybeansPlant DiseasesArgentinaCercospora species cause cercospora leaf blight (CLB) and purple seed stain (PSS) on soybean. Because there are few resistant soybean varieties available, CLB/PSS management relies heavily upon fungicide applications. Sensitivity of 62 Argentinian Cercospora isolates to demethylation inhibitor (DMI), methyl benzimidazole carbamate (MBC), quinone outside inhibitor (QoI), succinate dehydrogenase inhibitor (SDHI) fungicides, and mancozeb was determined in this study. All isolates were sensitive to difenoconazole, epoxiconazole, prothioconazole, tebuconazole, and cyproconazole (EC50 values ranged from 0.006 to 2.4 µg/ml). In contrast, 51% of the tested isolates were sensitive (EC50 values ranged from 0.003 to 0.2 µg/ml), and 49% were highly resistant (EC50 > 100 µg/ml) to carbendazim. Interestingly, all isolates were completely resistant to azoxystrobin, trifloxystrobin, and pyraclostrobin, and insensitive to boscalid, fluxapyroxad, and pydiflumetofen (EC50 > 100 µg/ml). The G143A mutation was detected in 82% (53) of the QoI-resistant isolates and the E198A mutation in 97% (31) of the carbendazim-resistant isolates. No apparent resistance mutations were detected in the succinate dehydrogenase genes (subunits sdhB, sdhC, and sdhD). Mancozeb completely inhibited mycelial growth of the isolates evaluated at a concentration of 100 µg/ml. All Argentinian Cercospora isolates were sensitive to the DMI fungicides tested, but we report for the first time resistance to QoI and MBC fungicides. Mechanism(s) other than fungicide target-site modification may be responsible for resistance of Cercospora to QoI and MBC fungicides. Moreover, based on our results and on the recent introduction of SDHI fungicides on soybean in Argentina, Cercospora species causing CLB/PSS are insensitive (naturally resistant) to SDHI fungicides. Insensitivity must be confirmed under field conditions.Instituto de BiotecnologíaFil: Sautua, Francisco José. Universidad de Buenos Aires. Facultad de Agronomía. Cátedra de Fitopatología; ArgentinaFil: Doyle, Vinson P. LSU AgCenter. Department of Plant Pathology and Crop Physiology; Estados UnidosFil: Price, Paul P. LSU AgCenter. Macon Ridge Research Station; Estados UnidosFil: Porfiri, Alejandro. Consultor independiente; ArgentinaFil: Fernandez, Paula Del Carmen. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina.Fil: Fernandez, Paula Del Carmen. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Scandiani, María Mercedes. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Centro de Referencia de Micología (CEREMIC); ArgentinaFil: Carmona, Marcelo Anibal. Universidad de Buenos Aires. Facultad de Agronomía. Cátedra de Fitopatología; ArgentinaWiley2022-01-14T11:19:52Z2022-01-14T11:19:52Z2020-12info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://hdl.handle.net/20.500.12123/11121https://bsppjournals.onlinelibrary.wiley.com/doi/abs/10.1111/ppa.132610032-08621365-3059https://doi.org/10.1111/ppa.13261Plant Pathology 69 (9) : 1678-1694 (December 2020)reponame:INTA Digital (INTA)instname:Instituto Nacional de Tecnología Agropecuariaenginfo:eu-repo/semantics/restrictedAccess2025-09-29T13:45:27Zoai:localhost:20.500.12123/11121instacron:INTAInstitucionalhttp://repositorio.inta.gob.ar/Organismo científico-tecnológicoNo correspondehttp://repositorio.inta.gob.ar/oai/requesttripaldi.nicolas@inta.gob.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:l2025-09-29 13:45:27.806INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuariafalse
dc.title.none.fl_str_mv Fungicide resistance in Cercospora species causing cercospora leaf blight and purple seed stain of soybean in Argentina
title Fungicide resistance in Cercospora species causing cercospora leaf blight and purple seed stain of soybean in Argentina
spellingShingle Fungicide resistance in Cercospora species causing cercospora leaf blight and purple seed stain of soybean in Argentina
Sautua, Francisco José
Cercospora
Fungicidas
Resistencia a los Fungicidas
Soja
Enfermedades de las Plantas
Fungicides
Resistance to Fungicides
Soybeans
Plant Diseases
Argentina
title_short Fungicide resistance in Cercospora species causing cercospora leaf blight and purple seed stain of soybean in Argentina
title_full Fungicide resistance in Cercospora species causing cercospora leaf blight and purple seed stain of soybean in Argentina
title_fullStr Fungicide resistance in Cercospora species causing cercospora leaf blight and purple seed stain of soybean in Argentina
title_full_unstemmed Fungicide resistance in Cercospora species causing cercospora leaf blight and purple seed stain of soybean in Argentina
title_sort Fungicide resistance in Cercospora species causing cercospora leaf blight and purple seed stain of soybean in Argentina
dc.creator.none.fl_str_mv Sautua, Francisco José
Doyle, Vinson P.
Price, Paul P.
Porfiri, Alejandro
Fernandez, Paula Del Carmen
Scandiani, María Mercedes
Carmona, Marcelo Anibal
author Sautua, Francisco José
author_facet Sautua, Francisco José
Doyle, Vinson P.
Price, Paul P.
Porfiri, Alejandro
Fernandez, Paula Del Carmen
Scandiani, María Mercedes
Carmona, Marcelo Anibal
author_role author
author2 Doyle, Vinson P.
Price, Paul P.
Porfiri, Alejandro
Fernandez, Paula Del Carmen
Scandiani, María Mercedes
Carmona, Marcelo Anibal
author2_role author
author
author
author
author
author
dc.subject.none.fl_str_mv Cercospora
Fungicidas
Resistencia a los Fungicidas
Soja
Enfermedades de las Plantas
Fungicides
Resistance to Fungicides
Soybeans
Plant Diseases
Argentina
topic Cercospora
Fungicidas
Resistencia a los Fungicidas
Soja
Enfermedades de las Plantas
Fungicides
Resistance to Fungicides
Soybeans
Plant Diseases
Argentina
dc.description.none.fl_txt_mv Cercospora species cause cercospora leaf blight (CLB) and purple seed stain (PSS) on soybean. Because there are few resistant soybean varieties available, CLB/PSS management relies heavily upon fungicide applications. Sensitivity of 62 Argentinian Cercospora isolates to demethylation inhibitor (DMI), methyl benzimidazole carbamate (MBC), quinone outside inhibitor (QoI), succinate dehydrogenase inhibitor (SDHI) fungicides, and mancozeb was determined in this study. All isolates were sensitive to difenoconazole, epoxiconazole, prothioconazole, tebuconazole, and cyproconazole (EC50 values ranged from 0.006 to 2.4 µg/ml). In contrast, 51% of the tested isolates were sensitive (EC50 values ranged from 0.003 to 0.2 µg/ml), and 49% were highly resistant (EC50 > 100 µg/ml) to carbendazim. Interestingly, all isolates were completely resistant to azoxystrobin, trifloxystrobin, and pyraclostrobin, and insensitive to boscalid, fluxapyroxad, and pydiflumetofen (EC50 > 100 µg/ml). The G143A mutation was detected in 82% (53) of the QoI-resistant isolates and the E198A mutation in 97% (31) of the carbendazim-resistant isolates. No apparent resistance mutations were detected in the succinate dehydrogenase genes (subunits sdhB, sdhC, and sdhD). Mancozeb completely inhibited mycelial growth of the isolates evaluated at a concentration of 100 µg/ml. All Argentinian Cercospora isolates were sensitive to the DMI fungicides tested, but we report for the first time resistance to QoI and MBC fungicides. Mechanism(s) other than fungicide target-site modification may be responsible for resistance of Cercospora to QoI and MBC fungicides. Moreover, based on our results and on the recent introduction of SDHI fungicides on soybean in Argentina, Cercospora species causing CLB/PSS are insensitive (naturally resistant) to SDHI fungicides. Insensitivity must be confirmed under field conditions.
Instituto de Biotecnología
Fil: Sautua, Francisco José. Universidad de Buenos Aires. Facultad de Agronomía. Cátedra de Fitopatología; Argentina
Fil: Doyle, Vinson P. LSU AgCenter. Department of Plant Pathology and Crop Physiology; Estados Unidos
Fil: Price, Paul P. LSU AgCenter. Macon Ridge Research Station; Estados Unidos
Fil: Porfiri, Alejandro. Consultor independiente; Argentina
Fil: Fernandez, Paula Del Carmen. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina.
Fil: Fernandez, Paula Del Carmen. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Scandiani, María Mercedes. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Centro de Referencia de Micología (CEREMIC); Argentina
Fil: Carmona, Marcelo Anibal. Universidad de Buenos Aires. Facultad de Agronomía. Cátedra de Fitopatología; Argentina
description Cercospora species cause cercospora leaf blight (CLB) and purple seed stain (PSS) on soybean. Because there are few resistant soybean varieties available, CLB/PSS management relies heavily upon fungicide applications. Sensitivity of 62 Argentinian Cercospora isolates to demethylation inhibitor (DMI), methyl benzimidazole carbamate (MBC), quinone outside inhibitor (QoI), succinate dehydrogenase inhibitor (SDHI) fungicides, and mancozeb was determined in this study. All isolates were sensitive to difenoconazole, epoxiconazole, prothioconazole, tebuconazole, and cyproconazole (EC50 values ranged from 0.006 to 2.4 µg/ml). In contrast, 51% of the tested isolates were sensitive (EC50 values ranged from 0.003 to 0.2 µg/ml), and 49% were highly resistant (EC50 > 100 µg/ml) to carbendazim. Interestingly, all isolates were completely resistant to azoxystrobin, trifloxystrobin, and pyraclostrobin, and insensitive to boscalid, fluxapyroxad, and pydiflumetofen (EC50 > 100 µg/ml). The G143A mutation was detected in 82% (53) of the QoI-resistant isolates and the E198A mutation in 97% (31) of the carbendazim-resistant isolates. No apparent resistance mutations were detected in the succinate dehydrogenase genes (subunits sdhB, sdhC, and sdhD). Mancozeb completely inhibited mycelial growth of the isolates evaluated at a concentration of 100 µg/ml. All Argentinian Cercospora isolates were sensitive to the DMI fungicides tested, but we report for the first time resistance to QoI and MBC fungicides. Mechanism(s) other than fungicide target-site modification may be responsible for resistance of Cercospora to QoI and MBC fungicides. Moreover, based on our results and on the recent introduction of SDHI fungicides on soybean in Argentina, Cercospora species causing CLB/PSS are insensitive (naturally resistant) to SDHI fungicides. Insensitivity must be confirmed under field conditions.
publishDate 2020
dc.date.none.fl_str_mv 2020-12
2022-01-14T11:19:52Z
2022-01-14T11:19:52Z
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/20.500.12123/11121
https://bsppjournals.onlinelibrary.wiley.com/doi/abs/10.1111/ppa.13261
0032-0862
1365-3059
https://doi.org/10.1111/ppa.13261
url http://hdl.handle.net/20.500.12123/11121
https://bsppjournals.onlinelibrary.wiley.com/doi/abs/10.1111/ppa.13261
https://doi.org/10.1111/ppa.13261
identifier_str_mv 0032-0862
1365-3059
dc.language.none.fl_str_mv eng
language eng
dc.rights.none.fl_str_mv info:eu-repo/semantics/restrictedAccess
eu_rights_str_mv restrictedAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Wiley
publisher.none.fl_str_mv Wiley
dc.source.none.fl_str_mv Plant Pathology 69 (9) : 1678-1694 (December 2020)
reponame:INTA Digital (INTA)
instname:Instituto Nacional de Tecnología Agropecuaria
reponame_str INTA Digital (INTA)
collection INTA Digital (INTA)
instname_str Instituto Nacional de Tecnología Agropecuaria
repository.name.fl_str_mv INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuaria
repository.mail.fl_str_mv tripaldi.nicolas@inta.gob.ar
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