Detection and genome characterization of beet mosaic virus naturally infecting fodder beet crop in Argentina

Autores
Luciani, Cecilia Elizabeth; Celli, Marcos Giovani; Brugo Carivali, María Florencia; Bongiorno, Vanina Aylén; Conci, Luis Rogelio; Fernandez, Franco Daniel; Perotto, Maria Cecilia
Año de publicación
2026
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Fodder beet (Beta vulgaris subsp. vulgaris) is cultivated due its tolerance to low temperatures and poor soils as it improves the structure of forage production systems. Viral diseases represent a significant threat to beet production. Beet mosaic virus (BtMV) is the only confirmed potyvirus infecting beets. Between 2023 and 2024, plants showing virus-like symptoms from Argentinian main production area were surveyed. Transmission electron microscopy revealed flexuous filamentous particles consistent with BtMV. Oxford Nanopore Technologies sequencing of one sample yielded the complete BtMV genome (B12AR; 9451 nt, excluding the poly (A) tail), sharing 97.47 % amino acid identity with the BtMV-Wa isolate from the USA. Additionally, sequences of Beet cryptic virus 2, a Deltapartitivirus, were detected. RT-PCR confirmed BtMV infection in four of seven samples collected. These results would indicate the presence of other viruses not identified in this study. Phylogenetic analysis grouped B12AR within a clade with isolates from USA and China, distinct from a divergent lineage infecting wild beets. A recombination event involving P1 and HC-Pro regions, with parental sequences from the USA and South Africa was detected. Further studies are necessary to determine the etiology of fodder beet symptoms. This is the first report and complete genome sequence of BtMV infecting fodder beet in Argentina.
Instituto de Patologìa Vegetal
Fil: Luciani, Cecilia Elizabeth. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina
Fil:Luciani, Cecilia Elizabeth . Consejo Nacional de Investigaciones Científicas y Técnicas. Unidad de Fitopatología y Modelización Agrícola (UFyMA); Argentina
Fil: Celli, Marcos Giovani. Consejo Nacional de Investigaciones Científicas y Técnicas. Unidad de Fitopatología y Modelización Agrícola (UFyMA); Argentina
Fil: Celli, Marcos Giovani. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina
Fil: Brugo Carivali, María Florencia. Consejo Nacional de Investigaciones Científicas y Técnicas. Unidad de Fitopatología y Modelización Agrícola (UFyMA); Argentina
Fil: Brugo Carivali, María Florencia. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina
Fil: Bongiorno, Vanina Aylén. Consejo Nacional de Investigaciones Científicas y Técnicas. Unidad de Fitopatología y Modelización Agrícola (UFyMA); Argentina
Fil: Bongiorno, Vanina Aylén. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina
Fil: Conci, Luis Rogelio. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina
Fil: Fernandez, Franco Daniel. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina
Fil: Fernandez, Franco Daniel. Consejo Nacional de Investigaciones Científicas y Técnicas. Unidad de Fitopatología y Modelización Agrícola (UFyMA); Argentina
Fil: Perotto, Maria Cecilia. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina
Fil: Perotto, Maria Cecilia. Consejo Nacional de Investigaciones Científicas y Técnicas. Unidad de Fitopatología y Modelización Agrícola (UFyMA); Argentina
Fuente
Crop Protection 202 : 107529 (April 2026)
Materia
Phylogeny
Recombination
Mangolds
Filogenia
Recombinación
Argentina
Remolacha Forrajera
BtMV
ONT
Fodder Beet
Nivel de accesibilidad
acceso restringido
Condiciones de uso
http://creativecommons.org/licenses/by-nc-sa/4.0/
Repositorio
INTA Digital (INTA)
Institución
Instituto Nacional de Tecnología Agropecuaria
OAI Identificador
oai:localhost:20.500.12123/25872
Acceder
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oai_identifier_str oai:localhost:20.500.12123/25872
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network_name_str INTA Digital (INTA)
spelling Detection and genome characterization of beet mosaic virus naturally infecting fodder beet crop in ArgentinaLuciani, Cecilia ElizabethCelli, Marcos GiovaniBrugo Carivali, María FlorenciaBongiorno, Vanina AylénConci, Luis RogelioFernandez, Franco DanielPerotto, Maria CeciliaPhylogenyRecombinationMangoldsFilogeniaRecombinaciónArgentinaRemolacha ForrajeraBtMVONTFodder BeetFodder beet (Beta vulgaris subsp. vulgaris) is cultivated due its tolerance to low temperatures and poor soils as it improves the structure of forage production systems. Viral diseases represent a significant threat to beet production. Beet mosaic virus (BtMV) is the only confirmed potyvirus infecting beets. Between 2023 and 2024, plants showing virus-like symptoms from Argentinian main production area were surveyed. Transmission electron microscopy revealed flexuous filamentous particles consistent with BtMV. Oxford Nanopore Technologies sequencing of one sample yielded the complete BtMV genome (B12AR; 9451 nt, excluding the poly (A) tail), sharing 97.47 % amino acid identity with the BtMV-Wa isolate from the USA. Additionally, sequences of Beet cryptic virus 2, a Deltapartitivirus, were detected. RT-PCR confirmed BtMV infection in four of seven samples collected. These results would indicate the presence of other viruses not identified in this study. Phylogenetic analysis grouped B12AR within a clade with isolates from USA and China, distinct from a divergent lineage infecting wild beets. A recombination event involving P1 and HC-Pro regions, with parental sequences from the USA and South Africa was detected. Further studies are necessary to determine the etiology of fodder beet symptoms. This is the first report and complete genome sequence of BtMV infecting fodder beet in Argentina.Instituto de Patologìa VegetalFil: Luciani, Cecilia Elizabeth. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; ArgentinaFil:Luciani, Cecilia Elizabeth . Consejo Nacional de Investigaciones Científicas y Técnicas. Unidad de Fitopatología y Modelización Agrícola (UFyMA); ArgentinaFil: Celli, Marcos Giovani. Consejo Nacional de Investigaciones Científicas y Técnicas. Unidad de Fitopatología y Modelización Agrícola (UFyMA); ArgentinaFil: Celli, Marcos Giovani. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; ArgentinaFil: Brugo Carivali, María Florencia. Consejo Nacional de Investigaciones Científicas y Técnicas. Unidad de Fitopatología y Modelización Agrícola (UFyMA); ArgentinaFil: Brugo Carivali, María Florencia. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; ArgentinaFil: Bongiorno, Vanina Aylén. Consejo Nacional de Investigaciones Científicas y Técnicas. Unidad de Fitopatología y Modelización Agrícola (UFyMA); ArgentinaFil: Bongiorno, Vanina Aylén. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; ArgentinaFil: Conci, Luis Rogelio. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; ArgentinaFil: Fernandez, Franco Daniel. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; ArgentinaFil: Fernandez, Franco Daniel. Consejo Nacional de Investigaciones Científicas y Técnicas. Unidad de Fitopatología y Modelización Agrícola (UFyMA); ArgentinaFil: Perotto, Maria Cecilia. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; ArgentinaFil: Perotto, Maria Cecilia. Consejo Nacional de Investigaciones Científicas y Técnicas. Unidad de Fitopatología y Modelización Agrícola (UFyMA); ArgentinaElsevier2026-04-21T10:11:20Z2026-04-21T10:11:20Z2026-04info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://hdl.handle.net/20.500.12123/25872https://www.sciencedirect.com/science/article/abs/pii/S02612194250042110261-21941873-6904https://doi.org/10.1016/j.cropro.2025.107529Crop Protection 202 : 107529 (April 2026)reponame:INTA Digital (INTA)instname:Instituto Nacional de Tecnología Agropecuariaenginfo:eu-repograntAgreement/INTA/2023-PD-L01-I074, Bases ecológicas y epidemiológicas para el diseño de estrategias de manejo de plagas agrícolas y forestalesinfo:eu-repo/semantics/restrictedAccesshttp://creativecommons.org/licenses/by-nc-sa/4.0/Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)2026-05-07T11:53:18Zoai:localhost:20.500.12123/25872instacron:INTAInstitucionalhttp://repositorio.inta.gob.ar/Organismo científico-tecnológicoNo correspondehttp://repositorio.inta.gob.ar/oai/requesttripaldi.nicolas@inta.gob.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:l2026-05-07 11:53:18.903INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuariafalse
dc.title.none.fl_str_mv Detection and genome characterization of beet mosaic virus naturally infecting fodder beet crop in Argentina
title Detection and genome characterization of beet mosaic virus naturally infecting fodder beet crop in Argentina
spellingShingle Detection and genome characterization of beet mosaic virus naturally infecting fodder beet crop in Argentina
Luciani, Cecilia Elizabeth
Phylogeny
Recombination
Mangolds
Filogenia
Recombinación
Argentina
Remolacha Forrajera
BtMV
ONT
Fodder Beet
title_short Detection and genome characterization of beet mosaic virus naturally infecting fodder beet crop in Argentina
title_full Detection and genome characterization of beet mosaic virus naturally infecting fodder beet crop in Argentina
title_fullStr Detection and genome characterization of beet mosaic virus naturally infecting fodder beet crop in Argentina
title_full_unstemmed Detection and genome characterization of beet mosaic virus naturally infecting fodder beet crop in Argentina
title_sort Detection and genome characterization of beet mosaic virus naturally infecting fodder beet crop in Argentina
dc.creator.none.fl_str_mv Luciani, Cecilia Elizabeth
Celli, Marcos Giovani
Brugo Carivali, María Florencia
Bongiorno, Vanina Aylén
Conci, Luis Rogelio
Fernandez, Franco Daniel
Perotto, Maria Cecilia
author Luciani, Cecilia Elizabeth
author_facet Luciani, Cecilia Elizabeth
Celli, Marcos Giovani
Brugo Carivali, María Florencia
Bongiorno, Vanina Aylén
Conci, Luis Rogelio
Fernandez, Franco Daniel
Perotto, Maria Cecilia
author_role author
author2 Celli, Marcos Giovani
Brugo Carivali, María Florencia
Bongiorno, Vanina Aylén
Conci, Luis Rogelio
Fernandez, Franco Daniel
Perotto, Maria Cecilia
author2_role author
author
author
author
author
author
dc.subject.none.fl_str_mv Phylogeny
Recombination
Mangolds
Filogenia
Recombinación
Argentina
Remolacha Forrajera
BtMV
ONT
Fodder Beet
topic Phylogeny
Recombination
Mangolds
Filogenia
Recombinación
Argentina
Remolacha Forrajera
BtMV
ONT
Fodder Beet
dc.description.none.fl_txt_mv Fodder beet (Beta vulgaris subsp. vulgaris) is cultivated due its tolerance to low temperatures and poor soils as it improves the structure of forage production systems. Viral diseases represent a significant threat to beet production. Beet mosaic virus (BtMV) is the only confirmed potyvirus infecting beets. Between 2023 and 2024, plants showing virus-like symptoms from Argentinian main production area were surveyed. Transmission electron microscopy revealed flexuous filamentous particles consistent with BtMV. Oxford Nanopore Technologies sequencing of one sample yielded the complete BtMV genome (B12AR; 9451 nt, excluding the poly (A) tail), sharing 97.47 % amino acid identity with the BtMV-Wa isolate from the USA. Additionally, sequences of Beet cryptic virus 2, a Deltapartitivirus, were detected. RT-PCR confirmed BtMV infection in four of seven samples collected. These results would indicate the presence of other viruses not identified in this study. Phylogenetic analysis grouped B12AR within a clade with isolates from USA and China, distinct from a divergent lineage infecting wild beets. A recombination event involving P1 and HC-Pro regions, with parental sequences from the USA and South Africa was detected. Further studies are necessary to determine the etiology of fodder beet symptoms. This is the first report and complete genome sequence of BtMV infecting fodder beet in Argentina.
Instituto de Patologìa Vegetal
Fil: Luciani, Cecilia Elizabeth. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina
Fil:Luciani, Cecilia Elizabeth . Consejo Nacional de Investigaciones Científicas y Técnicas. Unidad de Fitopatología y Modelización Agrícola (UFyMA); Argentina
Fil: Celli, Marcos Giovani. Consejo Nacional de Investigaciones Científicas y Técnicas. Unidad de Fitopatología y Modelización Agrícola (UFyMA); Argentina
Fil: Celli, Marcos Giovani. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina
Fil: Brugo Carivali, María Florencia. Consejo Nacional de Investigaciones Científicas y Técnicas. Unidad de Fitopatología y Modelización Agrícola (UFyMA); Argentina
Fil: Brugo Carivali, María Florencia. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina
Fil: Bongiorno, Vanina Aylén. Consejo Nacional de Investigaciones Científicas y Técnicas. Unidad de Fitopatología y Modelización Agrícola (UFyMA); Argentina
Fil: Bongiorno, Vanina Aylén. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina
Fil: Conci, Luis Rogelio. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina
Fil: Fernandez, Franco Daniel. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina
Fil: Fernandez, Franco Daniel. Consejo Nacional de Investigaciones Científicas y Técnicas. Unidad de Fitopatología y Modelización Agrícola (UFyMA); Argentina
Fil: Perotto, Maria Cecilia. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina
Fil: Perotto, Maria Cecilia. Consejo Nacional de Investigaciones Científicas y Técnicas. Unidad de Fitopatología y Modelización Agrícola (UFyMA); Argentina
description Fodder beet (Beta vulgaris subsp. vulgaris) is cultivated due its tolerance to low temperatures and poor soils as it improves the structure of forage production systems. Viral diseases represent a significant threat to beet production. Beet mosaic virus (BtMV) is the only confirmed potyvirus infecting beets. Between 2023 and 2024, plants showing virus-like symptoms from Argentinian main production area were surveyed. Transmission electron microscopy revealed flexuous filamentous particles consistent with BtMV. Oxford Nanopore Technologies sequencing of one sample yielded the complete BtMV genome (B12AR; 9451 nt, excluding the poly (A) tail), sharing 97.47 % amino acid identity with the BtMV-Wa isolate from the USA. Additionally, sequences of Beet cryptic virus 2, a Deltapartitivirus, were detected. RT-PCR confirmed BtMV infection in four of seven samples collected. These results would indicate the presence of other viruses not identified in this study. Phylogenetic analysis grouped B12AR within a clade with isolates from USA and China, distinct from a divergent lineage infecting wild beets. A recombination event involving P1 and HC-Pro regions, with parental sequences from the USA and South Africa was detected. Further studies are necessary to determine the etiology of fodder beet symptoms. This is the first report and complete genome sequence of BtMV infecting fodder beet in Argentina.
publishDate 2026
dc.date.none.fl_str_mv 2026-04-21T10:11:20Z
2026-04-21T10:11:20Z
2026-04
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/20.500.12123/25872
https://www.sciencedirect.com/science/article/abs/pii/S0261219425004211
0261-2194
1873-6904
https://doi.org/10.1016/j.cropro.2025.107529
url http://hdl.handle.net/20.500.12123/25872
https://www.sciencedirect.com/science/article/abs/pii/S0261219425004211
https://doi.org/10.1016/j.cropro.2025.107529
identifier_str_mv 0261-2194
1873-6904
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repograntAgreement/INTA/2023-PD-L01-I074, Bases ecológicas y epidemiológicas para el diseño de estrategias de manejo de plagas agrícolas y forestales
dc.rights.none.fl_str_mv info:eu-repo/semantics/restrictedAccess
http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
eu_rights_str_mv restrictedAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv Crop Protection 202 : 107529 (April 2026)
reponame:INTA Digital (INTA)
instname:Instituto Nacional de Tecnología Agropecuaria
reponame_str INTA Digital (INTA)
collection INTA Digital (INTA)
instname_str Instituto Nacional de Tecnología Agropecuaria
repository.name.fl_str_mv INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuaria
repository.mail.fl_str_mv tripaldi.nicolas@inta.gob.ar
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