Complementary Sex Determination in the Parasitic Wasp Diachasmimorpha longicaudata

Autores
Carabajal Paladino, Leonale Suzel; Muntaabski, Irina; Lanzavecchia, Silvia Beatriz; Le Bagousse-Pinguet, Yoann; Viscarret, Mariana Mabel; Juri, Marianela Lucía; Fueyo Sánchez, Luciana; Papeschi, Alba Graciela; Cladera, Jorge Luis; Bressa, María José
Año de publicación
2015
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
We studied the sex determination in Diachasmimorpha longicaudata, a parasitoid braconid wasp widely used as biological control agent of fruit pest tephritid flies. We tested the complementary sex determination hypothesis (CSD) known in at least 60 species of Hymenoptera. According to CSD, male or female development depends on the allelic composition of one sex locus (single-locus CSD) or multiple sex loci (multiple-locus CSD). Hemizygote individuals are normal haploid males, and heterozygotes for at least one sex locus are normal diploid females, but homozygotes for all the sex loci are diploid males. In order to force the occurrence of diploid males in D. longicaudata, we established highly inbred lines and examined their offspring using chromosome counting, flow cytometry, and sex ratio analysis. We found that when mother-son crosses were studied, this wasp produced about 20% of diploid males out of the total male progeny. Our results suggest that this parasitoid may represent the second genus with multiple-locus CSD in Hymenoptera. Knowledge about the sex determination system in D. longicaudata is relevant for the improvement of mass rearing protocols of this species. This information also provides the necessary background for further investigations on the underlying molecular mechanisms of sex determination in this species, and a better insight into the evolution of this pathway in Hymenoptera in particular and insects in general.
Instituto de Genética
Fil: Carabajal Paladino, Leonale Suzel. Biology Centre CAS. Institute of Entomology; República Checa. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética; Argentina
Fil: Muntaabski, Irina Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética; Argentina
Fil: Lanzavecchia, Silvia Beatriz. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética; Argentina
Fil: Le Bagousse-Pinguet, Yoann. University of South Bohemia. Department of Botany; República Checa
Fil: Viscarret, Mariana Mabel. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Microbiología y Zoología Agrícola. Insectario de Investigaciones para Lucha Biológica; Argentina
Fil: Juri, Marianela Lucía. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Fueyo Sánchez, Luciana. Universidad Nacional de Luján. Instituto de Ecología y Desarrollo Sustentable; Argentina
Fil: Papeschi, Alba Graciela. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Ecología, Genética y Evolución; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Cladera, Jorge Luis. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética; Argentina
Fil: Bressa, Maria Jose. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Ecología, Genética y Evolución de Buenos Aires. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Ecología, Genética y Evolución de Buenos Aires; Argentina
Fuente
PLoS ONE 10 (3) : e0119619. (March 2015)
Materia
Biosteres longicaudatus
Hymenoptera
Parasitoides
Tephritidae
Control de Plagas
Control Biológico
Parasitoids
Pest Control
Biological Control
Diachasmimorpha Longicaudata
Avispas
Nivel de accesibilidad
acceso abierto
Condiciones de uso
http://creativecommons.org/licenses/by-nc-sa/4.0/
Repositorio
INTA Digital (INTA)
Institución
Instituto Nacional de Tecnología Agropecuaria
OAI Identificador
oai:localhost:20.500.12123/5389
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oai_identifier_str oai:localhost:20.500.12123/5389
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network_name_str INTA Digital (INTA)
spelling Complementary Sex Determination in the Parasitic Wasp Diachasmimorpha longicaudataCarabajal Paladino, Leonale SuzelMuntaabski, IrinaLanzavecchia, Silvia BeatrizLe Bagousse-Pinguet, YoannViscarret, Mariana MabelJuri, Marianela LucíaFueyo Sánchez, LucianaPapeschi, Alba GracielaCladera, Jorge LuisBressa, María JoséBiosteres longicaudatusHymenopteraParasitoidesTephritidaeControl de PlagasControl BiológicoParasitoidsPest ControlBiological ControlDiachasmimorpha LongicaudataAvispasWe studied the sex determination in Diachasmimorpha longicaudata, a parasitoid braconid wasp widely used as biological control agent of fruit pest tephritid flies. We tested the complementary sex determination hypothesis (CSD) known in at least 60 species of Hymenoptera. According to CSD, male or female development depends on the allelic composition of one sex locus (single-locus CSD) or multiple sex loci (multiple-locus CSD). Hemizygote individuals are normal haploid males, and heterozygotes for at least one sex locus are normal diploid females, but homozygotes for all the sex loci are diploid males. In order to force the occurrence of diploid males in D. longicaudata, we established highly inbred lines and examined their offspring using chromosome counting, flow cytometry, and sex ratio analysis. We found that when mother-son crosses were studied, this wasp produced about 20% of diploid males out of the total male progeny. Our results suggest that this parasitoid may represent the second genus with multiple-locus CSD in Hymenoptera. Knowledge about the sex determination system in D. longicaudata is relevant for the improvement of mass rearing protocols of this species. This information also provides the necessary background for further investigations on the underlying molecular mechanisms of sex determination in this species, and a better insight into the evolution of this pathway in Hymenoptera in particular and insects in general.Instituto de GenéticaFil: Carabajal Paladino, Leonale Suzel. Biology Centre CAS. Institute of Entomology; República Checa. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética; ArgentinaFil: Muntaabski, Irina Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética; ArgentinaFil: Lanzavecchia, Silvia Beatriz. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética; ArgentinaFil: Le Bagousse-Pinguet, Yoann. University of South Bohemia. Department of Botany; República ChecaFil: Viscarret, Mariana Mabel. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Microbiología y Zoología Agrícola. Insectario de Investigaciones para Lucha Biológica; ArgentinaFil: Juri, Marianela Lucía. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Fueyo Sánchez, Luciana. Universidad Nacional de Luján. Instituto de Ecología y Desarrollo Sustentable; ArgentinaFil: Papeschi, Alba Graciela. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Ecología, Genética y Evolución; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Cladera, Jorge Luis. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética; ArgentinaFil: Bressa, Maria Jose. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Ecología, Genética y Evolución de Buenos Aires. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Ecología, Genética y Evolución de Buenos Aires; ArgentinaPlos One2019-06-28T13:55:31Z2019-06-28T13:55:31Z2015-03info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttps://journals.plos.org/plosone/article?id=10.1371/journal.pone.0119619http://hdl.handle.net/20.500.12123/53891932-6203https://doi.org/10.1371/journal.pone.0119619PLoS ONE 10 (3) : e0119619. (March 2015)reponame:INTA Digital (INTA)instname:Instituto Nacional de Tecnología Agropecuariaenginfo:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by-nc-sa/4.0/Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)2025-10-23T11:16:59Zoai:localhost:20.500.12123/5389instacron:INTAInstitucionalhttp://repositorio.inta.gob.ar/Organismo científico-tecnológicoNo correspondehttp://repositorio.inta.gob.ar/oai/requesttripaldi.nicolas@inta.gob.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:l2025-10-23 11:16:59.825INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuariafalse
dc.title.none.fl_str_mv Complementary Sex Determination in the Parasitic Wasp Diachasmimorpha longicaudata
title Complementary Sex Determination in the Parasitic Wasp Diachasmimorpha longicaudata
spellingShingle Complementary Sex Determination in the Parasitic Wasp Diachasmimorpha longicaudata
Carabajal Paladino, Leonale Suzel
Biosteres longicaudatus
Hymenoptera
Parasitoides
Tephritidae
Control de Plagas
Control Biológico
Parasitoids
Pest Control
Biological Control
Diachasmimorpha Longicaudata
Avispas
title_short Complementary Sex Determination in the Parasitic Wasp Diachasmimorpha longicaudata
title_full Complementary Sex Determination in the Parasitic Wasp Diachasmimorpha longicaudata
title_fullStr Complementary Sex Determination in the Parasitic Wasp Diachasmimorpha longicaudata
title_full_unstemmed Complementary Sex Determination in the Parasitic Wasp Diachasmimorpha longicaudata
title_sort Complementary Sex Determination in the Parasitic Wasp Diachasmimorpha longicaudata
dc.creator.none.fl_str_mv Carabajal Paladino, Leonale Suzel
Muntaabski, Irina
Lanzavecchia, Silvia Beatriz
Le Bagousse-Pinguet, Yoann
Viscarret, Mariana Mabel
Juri, Marianela Lucía
Fueyo Sánchez, Luciana
Papeschi, Alba Graciela
Cladera, Jorge Luis
Bressa, María José
author Carabajal Paladino, Leonale Suzel
author_facet Carabajal Paladino, Leonale Suzel
Muntaabski, Irina
Lanzavecchia, Silvia Beatriz
Le Bagousse-Pinguet, Yoann
Viscarret, Mariana Mabel
Juri, Marianela Lucía
Fueyo Sánchez, Luciana
Papeschi, Alba Graciela
Cladera, Jorge Luis
Bressa, María José
author_role author
author2 Muntaabski, Irina
Lanzavecchia, Silvia Beatriz
Le Bagousse-Pinguet, Yoann
Viscarret, Mariana Mabel
Juri, Marianela Lucía
Fueyo Sánchez, Luciana
Papeschi, Alba Graciela
Cladera, Jorge Luis
Bressa, María José
author2_role author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Biosteres longicaudatus
Hymenoptera
Parasitoides
Tephritidae
Control de Plagas
Control Biológico
Parasitoids
Pest Control
Biological Control
Diachasmimorpha Longicaudata
Avispas
topic Biosteres longicaudatus
Hymenoptera
Parasitoides
Tephritidae
Control de Plagas
Control Biológico
Parasitoids
Pest Control
Biological Control
Diachasmimorpha Longicaudata
Avispas
dc.description.none.fl_txt_mv We studied the sex determination in Diachasmimorpha longicaudata, a parasitoid braconid wasp widely used as biological control agent of fruit pest tephritid flies. We tested the complementary sex determination hypothesis (CSD) known in at least 60 species of Hymenoptera. According to CSD, male or female development depends on the allelic composition of one sex locus (single-locus CSD) or multiple sex loci (multiple-locus CSD). Hemizygote individuals are normal haploid males, and heterozygotes for at least one sex locus are normal diploid females, but homozygotes for all the sex loci are diploid males. In order to force the occurrence of diploid males in D. longicaudata, we established highly inbred lines and examined their offspring using chromosome counting, flow cytometry, and sex ratio analysis. We found that when mother-son crosses were studied, this wasp produced about 20% of diploid males out of the total male progeny. Our results suggest that this parasitoid may represent the second genus with multiple-locus CSD in Hymenoptera. Knowledge about the sex determination system in D. longicaudata is relevant for the improvement of mass rearing protocols of this species. This information also provides the necessary background for further investigations on the underlying molecular mechanisms of sex determination in this species, and a better insight into the evolution of this pathway in Hymenoptera in particular and insects in general.
Instituto de Genética
Fil: Carabajal Paladino, Leonale Suzel. Biology Centre CAS. Institute of Entomology; República Checa. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética; Argentina
Fil: Muntaabski, Irina Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética; Argentina
Fil: Lanzavecchia, Silvia Beatriz. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética; Argentina
Fil: Le Bagousse-Pinguet, Yoann. University of South Bohemia. Department of Botany; República Checa
Fil: Viscarret, Mariana Mabel. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Microbiología y Zoología Agrícola. Insectario de Investigaciones para Lucha Biológica; Argentina
Fil: Juri, Marianela Lucía. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Fueyo Sánchez, Luciana. Universidad Nacional de Luján. Instituto de Ecología y Desarrollo Sustentable; Argentina
Fil: Papeschi, Alba Graciela. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Ecología, Genética y Evolución; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Cladera, Jorge Luis. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética; Argentina
Fil: Bressa, Maria Jose. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Ecología, Genética y Evolución de Buenos Aires. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Ecología, Genética y Evolución de Buenos Aires; Argentina
description We studied the sex determination in Diachasmimorpha longicaudata, a parasitoid braconid wasp widely used as biological control agent of fruit pest tephritid flies. We tested the complementary sex determination hypothesis (CSD) known in at least 60 species of Hymenoptera. According to CSD, male or female development depends on the allelic composition of one sex locus (single-locus CSD) or multiple sex loci (multiple-locus CSD). Hemizygote individuals are normal haploid males, and heterozygotes for at least one sex locus are normal diploid females, but homozygotes for all the sex loci are diploid males. In order to force the occurrence of diploid males in D. longicaudata, we established highly inbred lines and examined their offspring using chromosome counting, flow cytometry, and sex ratio analysis. We found that when mother-son crosses were studied, this wasp produced about 20% of diploid males out of the total male progeny. Our results suggest that this parasitoid may represent the second genus with multiple-locus CSD in Hymenoptera. Knowledge about the sex determination system in D. longicaudata is relevant for the improvement of mass rearing protocols of this species. This information also provides the necessary background for further investigations on the underlying molecular mechanisms of sex determination in this species, and a better insight into the evolution of this pathway in Hymenoptera in particular and insects in general.
publishDate 2015
dc.date.none.fl_str_mv 2015-03
2019-06-28T13:55:31Z
2019-06-28T13:55:31Z
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0119619
http://hdl.handle.net/20.500.12123/5389
1932-6203
https://doi.org/10.1371/journal.pone.0119619
url https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0119619
http://hdl.handle.net/20.500.12123/5389
https://doi.org/10.1371/journal.pone.0119619
identifier_str_mv 1932-6203
dc.language.none.fl_str_mv eng
language eng
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
eu_rights_str_mv openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Plos One
publisher.none.fl_str_mv Plos One
dc.source.none.fl_str_mv PLoS ONE 10 (3) : e0119619. (March 2015)
reponame:INTA Digital (INTA)
instname:Instituto Nacional de Tecnología Agropecuaria
reponame_str INTA Digital (INTA)
collection INTA Digital (INTA)
instname_str Instituto Nacional de Tecnología Agropecuaria
repository.name.fl_str_mv INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuaria
repository.mail.fl_str_mv tripaldi.nicolas@inta.gob.ar
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