Vaccination against babesiosis using recombinant GPI-anchored proteins
- Autores
- Wieser, Sara Nathaly; Schnittger, Leonhard; Florin-Christensen, Monica; Delbecq, Stephane; Schetters, Theo
- Año de publicación
- 2019
- Idioma
- inglés
- Tipo de recurso
- artículo
- Estado
- versión publicada
- Descripción
- In Press, Corrected Proof
The increase in human babesiosis is of major concern to health authorities. In the USA, most of these cases are due to infections with Babesia microti, whereas in Europe B. divergens is the major cause of clinical disease in humans. Here we review the immunological and biological literature of glycosylphosphatidylinositol (GPI)-anchored merozoite proteins of human Babesia parasites with emphasis on their role in immunity, and provide some new bioinformatical information on B. microti GPI-Anchored Proteins (GPI-AP). Cattle can be vaccinated with soluble parasite antigens (SPA) of Babesia divergens that are released by the parasite during proliferation. The major component in SPA preparations appeared to be a 37 kDa merozoite surface protein that is anchored in the merozoite membrane by a GPI anchor. Animals could be protected by vaccination with the recombinant 37 kDa protein expressed in Escherichia coli, provided the protein had a hydrophobic terminal sequence. Based on this knowledge, a recombinant vaccine was developed against Babesia canis infection in dogs, successfully. In order to identify similar GPI-AP in B. microti, the genome was analysed. Here it is shown that B. microti encodes all proteins necessary for GPI assembly and its subsequent protein transfer. In addition, in total 21 genes encoding for GPI-AP were detected, some of which reacted particularly strongly with sera from B. microti-infected human patients. Reactivity of antibodies with GPI-anchored merozoite proteins appears to be dependent on the structural conformation of the molecule. It is suggested that the three-dimensional structure of the protein that is anchored in the membrane is different from that of the protein that has been shed from the merozoite surface. The significance of this protein’s dynamics in parasite biology and immune evasion is discussed. Finally, we discuss developments in tick and Babesia vaccine research, and the role such vaccines could play in the control of human babesiosis.
Instituto de Patobiología
Fil: Wieser, Sarah Nathaly. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Schnittger, Leonhard. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Florin-Christensen, Monica. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Delbecq, Stephane. Université de Montpellier.· Vaccination Antiparasitaire; Francia
Fil: Schetters, Theo. Université de Montpellier.· Vaccination Antiparasitaire; Francia. University of Pretoria. Veterinary Faculty. Department of Veterinary Tropical Diseases; Sudáfrica - Fuente
- International journal for parasitology (24 January 2019)
- Materia
-
Babesiosis
Vacunación
Babesia Divergens
Babesia Microti
Vacuna Sintética
Vaccination
Sporozoa
Synthetic Vaccines
Apicomplexa
GPI Anchors
Merozoite Surface Protein
Glycosylphosphatidylinositol - Nivel de accesibilidad
- acceso restringido
- Condiciones de uso
- Repositorio
- Institución
- Instituto Nacional de Tecnología Agropecuaria
- OAI Identificador
- oai:localhost:20.500.12123/4469
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Vaccination against babesiosis using recombinant GPI-anchored proteinsWieser, Sara NathalySchnittger, LeonhardFlorin-Christensen, MonicaDelbecq, StephaneSchetters, TheoBabesiosisVacunaciónBabesia DivergensBabesia MicrotiVacuna SintéticaVaccinationSporozoaSynthetic VaccinesApicomplexaGPI AnchorsMerozoite Surface ProteinGlycosylphosphatidylinositolIn Press, Corrected ProofThe increase in human babesiosis is of major concern to health authorities. In the USA, most of these cases are due to infections with Babesia microti, whereas in Europe B. divergens is the major cause of clinical disease in humans. Here we review the immunological and biological literature of glycosylphosphatidylinositol (GPI)-anchored merozoite proteins of human Babesia parasites with emphasis on their role in immunity, and provide some new bioinformatical information on B. microti GPI-Anchored Proteins (GPI-AP). Cattle can be vaccinated with soluble parasite antigens (SPA) of Babesia divergens that are released by the parasite during proliferation. The major component in SPA preparations appeared to be a 37 kDa merozoite surface protein that is anchored in the merozoite membrane by a GPI anchor. Animals could be protected by vaccination with the recombinant 37 kDa protein expressed in Escherichia coli, provided the protein had a hydrophobic terminal sequence. Based on this knowledge, a recombinant vaccine was developed against Babesia canis infection in dogs, successfully. In order to identify similar GPI-AP in B. microti, the genome was analysed. Here it is shown that B. microti encodes all proteins necessary for GPI assembly and its subsequent protein transfer. In addition, in total 21 genes encoding for GPI-AP were detected, some of which reacted particularly strongly with sera from B. microti-infected human patients. Reactivity of antibodies with GPI-anchored merozoite proteins appears to be dependent on the structural conformation of the molecule. It is suggested that the three-dimensional structure of the protein that is anchored in the membrane is different from that of the protein that has been shed from the merozoite surface. The significance of this protein’s dynamics in parasite biology and immune evasion is discussed. Finally, we discuss developments in tick and Babesia vaccine research, and the role such vaccines could play in the control of human babesiosis.Instituto de PatobiologíaFil: Wieser, Sarah Nathaly. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Schnittger, Leonhard. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Florin-Christensen, Monica. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Delbecq, Stephane. Université de Montpellier.· Vaccination Antiparasitaire; FranciaFil: Schetters, Theo. Université de Montpellier.· Vaccination Antiparasitaire; Francia. University of Pretoria. Veterinary Faculty. Department of Veterinary Tropical Diseases; SudáfricaElsevier2019-02-19T17:46:38Z2019-02-19T17:46:38Z2019-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://hdl.handle.net/20.500.12123/4469https://www.sciencedirect.com/science/article/pii/S0020751919300037?via%3Dihub0020-7519https://doi.org/10.1016/j.ijpara.2018.12.002International journal for parasitology (24 January 2019)reponame:INTA Digital (INTA)instname:Instituto Nacional de Tecnología Agropecuariaenginfo:eu-repo/semantics/restrictedAccess2025-09-04T09:47:48Zoai:localhost:20.500.12123/4469instacron:INTAInstitucionalhttp://repositorio.inta.gob.ar/Organismo científico-tecnológicoNo correspondehttp://repositorio.inta.gob.ar/oai/requesttripaldi.nicolas@inta.gob.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:l2025-09-04 09:47:49.374INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuariafalse |
dc.title.none.fl_str_mv |
Vaccination against babesiosis using recombinant GPI-anchored proteins |
title |
Vaccination against babesiosis using recombinant GPI-anchored proteins |
spellingShingle |
Vaccination against babesiosis using recombinant GPI-anchored proteins Wieser, Sara Nathaly Babesiosis Vacunación Babesia Divergens Babesia Microti Vacuna Sintética Vaccination Sporozoa Synthetic Vaccines Apicomplexa GPI Anchors Merozoite Surface Protein Glycosylphosphatidylinositol |
title_short |
Vaccination against babesiosis using recombinant GPI-anchored proteins |
title_full |
Vaccination against babesiosis using recombinant GPI-anchored proteins |
title_fullStr |
Vaccination against babesiosis using recombinant GPI-anchored proteins |
title_full_unstemmed |
Vaccination against babesiosis using recombinant GPI-anchored proteins |
title_sort |
Vaccination against babesiosis using recombinant GPI-anchored proteins |
dc.creator.none.fl_str_mv |
Wieser, Sara Nathaly Schnittger, Leonhard Florin-Christensen, Monica Delbecq, Stephane Schetters, Theo |
author |
Wieser, Sara Nathaly |
author_facet |
Wieser, Sara Nathaly Schnittger, Leonhard Florin-Christensen, Monica Delbecq, Stephane Schetters, Theo |
author_role |
author |
author2 |
Schnittger, Leonhard Florin-Christensen, Monica Delbecq, Stephane Schetters, Theo |
author2_role |
author author author author |
dc.subject.none.fl_str_mv |
Babesiosis Vacunación Babesia Divergens Babesia Microti Vacuna Sintética Vaccination Sporozoa Synthetic Vaccines Apicomplexa GPI Anchors Merozoite Surface Protein Glycosylphosphatidylinositol |
topic |
Babesiosis Vacunación Babesia Divergens Babesia Microti Vacuna Sintética Vaccination Sporozoa Synthetic Vaccines Apicomplexa GPI Anchors Merozoite Surface Protein Glycosylphosphatidylinositol |
dc.description.none.fl_txt_mv |
In Press, Corrected Proof The increase in human babesiosis is of major concern to health authorities. In the USA, most of these cases are due to infections with Babesia microti, whereas in Europe B. divergens is the major cause of clinical disease in humans. Here we review the immunological and biological literature of glycosylphosphatidylinositol (GPI)-anchored merozoite proteins of human Babesia parasites with emphasis on their role in immunity, and provide some new bioinformatical information on B. microti GPI-Anchored Proteins (GPI-AP). Cattle can be vaccinated with soluble parasite antigens (SPA) of Babesia divergens that are released by the parasite during proliferation. The major component in SPA preparations appeared to be a 37 kDa merozoite surface protein that is anchored in the merozoite membrane by a GPI anchor. Animals could be protected by vaccination with the recombinant 37 kDa protein expressed in Escherichia coli, provided the protein had a hydrophobic terminal sequence. Based on this knowledge, a recombinant vaccine was developed against Babesia canis infection in dogs, successfully. In order to identify similar GPI-AP in B. microti, the genome was analysed. Here it is shown that B. microti encodes all proteins necessary for GPI assembly and its subsequent protein transfer. In addition, in total 21 genes encoding for GPI-AP were detected, some of which reacted particularly strongly with sera from B. microti-infected human patients. Reactivity of antibodies with GPI-anchored merozoite proteins appears to be dependent on the structural conformation of the molecule. It is suggested that the three-dimensional structure of the protein that is anchored in the membrane is different from that of the protein that has been shed from the merozoite surface. The significance of this protein’s dynamics in parasite biology and immune evasion is discussed. Finally, we discuss developments in tick and Babesia vaccine research, and the role such vaccines could play in the control of human babesiosis. Instituto de Patobiología Fil: Wieser, Sarah Nathaly. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Schnittger, Leonhard. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Florin-Christensen, Monica. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Delbecq, Stephane. Université de Montpellier.· Vaccination Antiparasitaire; Francia Fil: Schetters, Theo. Université de Montpellier.· Vaccination Antiparasitaire; Francia. University of Pretoria. Veterinary Faculty. Department of Veterinary Tropical Diseases; Sudáfrica |
description |
In Press, Corrected Proof |
publishDate |
2019 |
dc.date.none.fl_str_mv |
2019-02-19T17:46:38Z 2019-02-19T17:46:38Z 2019-01 |
dc.type.none.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://purl.org/coar/resource_type/c_6501 info:ar-repo/semantics/articulo |
format |
article |
status_str |
publishedVersion |
dc.identifier.none.fl_str_mv |
http://hdl.handle.net/20.500.12123/4469 https://www.sciencedirect.com/science/article/pii/S0020751919300037?via%3Dihub 0020-7519 https://doi.org/10.1016/j.ijpara.2018.12.002 |
url |
http://hdl.handle.net/20.500.12123/4469 https://www.sciencedirect.com/science/article/pii/S0020751919300037?via%3Dihub https://doi.org/10.1016/j.ijpara.2018.12.002 |
identifier_str_mv |
0020-7519 |
dc.language.none.fl_str_mv |
eng |
language |
eng |
dc.rights.none.fl_str_mv |
info:eu-repo/semantics/restrictedAccess |
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restrictedAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Elsevier |
publisher.none.fl_str_mv |
Elsevier |
dc.source.none.fl_str_mv |
International journal for parasitology (24 January 2019) reponame:INTA Digital (INTA) instname:Instituto Nacional de Tecnología Agropecuaria |
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INTA Digital (INTA) |
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Instituto Nacional de Tecnología Agropecuaria |
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INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuaria |
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tripaldi.nicolas@inta.gob.ar |
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12.623145 |