Vaccination against babesiosis using recombinant GPI-anchored proteins

Autores
Wieser, Sara Nathaly; Schnittger, Leonhard; Florin-Christensen, Monica; Delbecq, Stephane; Schetters, Theo
Año de publicación
2019
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
In Press, Corrected Proof
The increase in human babesiosis is of major concern to health authorities. In the USA, most of these cases are due to infections with Babesia microti, whereas in Europe B. divergens is the major cause of clinical disease in humans. Here we review the immunological and biological literature of glycosylphosphatidylinositol (GPI)-anchored merozoite proteins of human Babesia parasites with emphasis on their role in immunity, and provide some new bioinformatical information on B. microti GPI-Anchored Proteins (GPI-AP). Cattle can be vaccinated with soluble parasite antigens (SPA) of Babesia divergens that are released by the parasite during proliferation. The major component in SPA preparations appeared to be a 37 kDa merozoite surface protein that is anchored in the merozoite membrane by a GPI anchor. Animals could be protected by vaccination with the recombinant 37 kDa protein expressed in Escherichia coli, provided the protein had a hydrophobic terminal sequence. Based on this knowledge, a recombinant vaccine was developed against Babesia canis infection in dogs, successfully. In order to identify similar GPI-AP in B. microti, the genome was analysed. Here it is shown that B. microti encodes all proteins necessary for GPI assembly and its subsequent protein transfer. In addition, in total 21 genes encoding for GPI-AP were detected, some of which reacted particularly strongly with sera from B. microti-infected human patients. Reactivity of antibodies with GPI-anchored merozoite proteins appears to be dependent on the structural conformation of the molecule. It is suggested that the three-dimensional structure of the protein that is anchored in the membrane is different from that of the protein that has been shed from the merozoite surface. The significance of this protein’s dynamics in parasite biology and immune evasion is discussed. Finally, we discuss developments in tick and Babesia vaccine research, and the role such vaccines could play in the control of human babesiosis.
Instituto de Patobiología
Fil: Wieser, Sarah Nathaly. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Schnittger, Leonhard. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Florin-Christensen, Monica. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Delbecq, Stephane. Université de Montpellier.· Vaccination Antiparasitaire; Francia
Fil: Schetters, Theo. Université de Montpellier.· Vaccination Antiparasitaire; Francia. University of Pretoria. Veterinary Faculty. Department of Veterinary Tropical Diseases; Sudáfrica
Fuente
International journal for parasitology (24 January 2019)
Materia
Babesiosis
Vacunación
Babesia Divergens
Babesia Microti
Vacuna Sintética
Vaccination
Sporozoa
Synthetic Vaccines
Apicomplexa
GPI Anchors
Merozoite Surface Protein
Glycosylphosphatidylinositol
Nivel de accesibilidad
acceso restringido
Condiciones de uso
Repositorio
INTA Digital (INTA)
Institución
Instituto Nacional de Tecnología Agropecuaria
OAI Identificador
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spelling Vaccination against babesiosis using recombinant GPI-anchored proteinsWieser, Sara NathalySchnittger, LeonhardFlorin-Christensen, MonicaDelbecq, StephaneSchetters, TheoBabesiosisVacunaciónBabesia DivergensBabesia MicrotiVacuna SintéticaVaccinationSporozoaSynthetic VaccinesApicomplexaGPI AnchorsMerozoite Surface ProteinGlycosylphosphatidylinositolIn Press, Corrected ProofThe increase in human babesiosis is of major concern to health authorities. In the USA, most of these cases are due to infections with Babesia microti, whereas in Europe B. divergens is the major cause of clinical disease in humans. Here we review the immunological and biological literature of glycosylphosphatidylinositol (GPI)-anchored merozoite proteins of human Babesia parasites with emphasis on their role in immunity, and provide some new bioinformatical information on B. microti GPI-Anchored Proteins (GPI-AP). Cattle can be vaccinated with soluble parasite antigens (SPA) of Babesia divergens that are released by the parasite during proliferation. The major component in SPA preparations appeared to be a 37 kDa merozoite surface protein that is anchored in the merozoite membrane by a GPI anchor. Animals could be protected by vaccination with the recombinant 37 kDa protein expressed in Escherichia coli, provided the protein had a hydrophobic terminal sequence. Based on this knowledge, a recombinant vaccine was developed against Babesia canis infection in dogs, successfully. In order to identify similar GPI-AP in B. microti, the genome was analysed. Here it is shown that B. microti encodes all proteins necessary for GPI assembly and its subsequent protein transfer. In addition, in total 21 genes encoding for GPI-AP were detected, some of which reacted particularly strongly with sera from B. microti-infected human patients. Reactivity of antibodies with GPI-anchored merozoite proteins appears to be dependent on the structural conformation of the molecule. It is suggested that the three-dimensional structure of the protein that is anchored in the membrane is different from that of the protein that has been shed from the merozoite surface. The significance of this protein’s dynamics in parasite biology and immune evasion is discussed. Finally, we discuss developments in tick and Babesia vaccine research, and the role such vaccines could play in the control of human babesiosis.Instituto de PatobiologíaFil: Wieser, Sarah Nathaly. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Schnittger, Leonhard. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Florin-Christensen, Monica. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Delbecq, Stephane. Université de Montpellier.· Vaccination Antiparasitaire; FranciaFil: Schetters, Theo. Université de Montpellier.· Vaccination Antiparasitaire; Francia. University of Pretoria. Veterinary Faculty. Department of Veterinary Tropical Diseases; SudáfricaElsevier2019-02-19T17:46:38Z2019-02-19T17:46:38Z2019-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://hdl.handle.net/20.500.12123/4469https://www.sciencedirect.com/science/article/pii/S0020751919300037?via%3Dihub0020-7519https://doi.org/10.1016/j.ijpara.2018.12.002International journal for parasitology (24 January 2019)reponame:INTA Digital (INTA)instname:Instituto Nacional de Tecnología Agropecuariaenginfo:eu-repo/semantics/restrictedAccess2025-09-04T09:47:48Zoai:localhost:20.500.12123/4469instacron:INTAInstitucionalhttp://repositorio.inta.gob.ar/Organismo científico-tecnológicoNo correspondehttp://repositorio.inta.gob.ar/oai/requesttripaldi.nicolas@inta.gob.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:l2025-09-04 09:47:49.374INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuariafalse
dc.title.none.fl_str_mv Vaccination against babesiosis using recombinant GPI-anchored proteins
title Vaccination against babesiosis using recombinant GPI-anchored proteins
spellingShingle Vaccination against babesiosis using recombinant GPI-anchored proteins
Wieser, Sara Nathaly
Babesiosis
Vacunación
Babesia Divergens
Babesia Microti
Vacuna Sintética
Vaccination
Sporozoa
Synthetic Vaccines
Apicomplexa
GPI Anchors
Merozoite Surface Protein
Glycosylphosphatidylinositol
title_short Vaccination against babesiosis using recombinant GPI-anchored proteins
title_full Vaccination against babesiosis using recombinant GPI-anchored proteins
title_fullStr Vaccination against babesiosis using recombinant GPI-anchored proteins
title_full_unstemmed Vaccination against babesiosis using recombinant GPI-anchored proteins
title_sort Vaccination against babesiosis using recombinant GPI-anchored proteins
dc.creator.none.fl_str_mv Wieser, Sara Nathaly
Schnittger, Leonhard
Florin-Christensen, Monica
Delbecq, Stephane
Schetters, Theo
author Wieser, Sara Nathaly
author_facet Wieser, Sara Nathaly
Schnittger, Leonhard
Florin-Christensen, Monica
Delbecq, Stephane
Schetters, Theo
author_role author
author2 Schnittger, Leonhard
Florin-Christensen, Monica
Delbecq, Stephane
Schetters, Theo
author2_role author
author
author
author
dc.subject.none.fl_str_mv Babesiosis
Vacunación
Babesia Divergens
Babesia Microti
Vacuna Sintética
Vaccination
Sporozoa
Synthetic Vaccines
Apicomplexa
GPI Anchors
Merozoite Surface Protein
Glycosylphosphatidylinositol
topic Babesiosis
Vacunación
Babesia Divergens
Babesia Microti
Vacuna Sintética
Vaccination
Sporozoa
Synthetic Vaccines
Apicomplexa
GPI Anchors
Merozoite Surface Protein
Glycosylphosphatidylinositol
dc.description.none.fl_txt_mv In Press, Corrected Proof
The increase in human babesiosis is of major concern to health authorities. In the USA, most of these cases are due to infections with Babesia microti, whereas in Europe B. divergens is the major cause of clinical disease in humans. Here we review the immunological and biological literature of glycosylphosphatidylinositol (GPI)-anchored merozoite proteins of human Babesia parasites with emphasis on their role in immunity, and provide some new bioinformatical information on B. microti GPI-Anchored Proteins (GPI-AP). Cattle can be vaccinated with soluble parasite antigens (SPA) of Babesia divergens that are released by the parasite during proliferation. The major component in SPA preparations appeared to be a 37 kDa merozoite surface protein that is anchored in the merozoite membrane by a GPI anchor. Animals could be protected by vaccination with the recombinant 37 kDa protein expressed in Escherichia coli, provided the protein had a hydrophobic terminal sequence. Based on this knowledge, a recombinant vaccine was developed against Babesia canis infection in dogs, successfully. In order to identify similar GPI-AP in B. microti, the genome was analysed. Here it is shown that B. microti encodes all proteins necessary for GPI assembly and its subsequent protein transfer. In addition, in total 21 genes encoding for GPI-AP were detected, some of which reacted particularly strongly with sera from B. microti-infected human patients. Reactivity of antibodies with GPI-anchored merozoite proteins appears to be dependent on the structural conformation of the molecule. It is suggested that the three-dimensional structure of the protein that is anchored in the membrane is different from that of the protein that has been shed from the merozoite surface. The significance of this protein’s dynamics in parasite biology and immune evasion is discussed. Finally, we discuss developments in tick and Babesia vaccine research, and the role such vaccines could play in the control of human babesiosis.
Instituto de Patobiología
Fil: Wieser, Sarah Nathaly. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Schnittger, Leonhard. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Florin-Christensen, Monica. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Delbecq, Stephane. Université de Montpellier.· Vaccination Antiparasitaire; Francia
Fil: Schetters, Theo. Université de Montpellier.· Vaccination Antiparasitaire; Francia. University of Pretoria. Veterinary Faculty. Department of Veterinary Tropical Diseases; Sudáfrica
description In Press, Corrected Proof
publishDate 2019
dc.date.none.fl_str_mv 2019-02-19T17:46:38Z
2019-02-19T17:46:38Z
2019-01
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/20.500.12123/4469
https://www.sciencedirect.com/science/article/pii/S0020751919300037?via%3Dihub
0020-7519
https://doi.org/10.1016/j.ijpara.2018.12.002
url http://hdl.handle.net/20.500.12123/4469
https://www.sciencedirect.com/science/article/pii/S0020751919300037?via%3Dihub
https://doi.org/10.1016/j.ijpara.2018.12.002
identifier_str_mv 0020-7519
dc.language.none.fl_str_mv eng
language eng
dc.rights.none.fl_str_mv info:eu-repo/semantics/restrictedAccess
eu_rights_str_mv restrictedAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv International journal for parasitology (24 January 2019)
reponame:INTA Digital (INTA)
instname:Instituto Nacional de Tecnología Agropecuaria
reponame_str INTA Digital (INTA)
collection INTA Digital (INTA)
instname_str Instituto Nacional de Tecnología Agropecuaria
repository.name.fl_str_mv INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuaria
repository.mail.fl_str_mv tripaldi.nicolas@inta.gob.ar
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