Babesia microti immunoreactive rhoptry-associated protein-1 paralogs are ancestral members of the piroplasmid-confined RAP-1 family

Autores
Bastos, Reginaldo; Thekkiniath, Jose; Ben Mamoun, Choukri; Fuller, Lee; Molestina, Robert E.; Florin-Christensen, Mónica; Schnittger, Leonhard; Alzan, Heba F.; Suarez, Carlos
Año de publicación
2021
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Babesia, Cytauxzoon and Theileria are tick-borne apicomplexan parasites of the order Piroplasmida, responsible for diseases in humans and animals. Members of the piroplasmid rhoptry-associated protein-1 (pRAP-1) family have a signature cysteine-rich domain and are important for parasite development. We propose that the closely linked B. microti genes annotated as BMR1_03g00947 and BMR1_03g00960 encode two paralogue pRAP-1-like proteins named BmIPA48 and Bm960. The two genes are tandemly arranged head to tail, highly expressed in blood stage parasites, syntenic to rap-1 genes of other piroplasmids, and share large portions of an almost identical ~225 bp sequence located in their 5′ putative regulatory regions. BmIPA48 and Bm960 proteins contain a N-terminal signal peptide, share very low sequence identity (<13%) with pRAP-1 from other species, and harbor one or more transmembrane domains. Diversification of the piroplasmid-confined prap-1 family is characterized by amplification of genes, protein domains, and a high sequence polymorphism. This suggests a functional involvement of pRAP-1 at the parasite-host interface, possibly in parasite adhesion, attachment, and/or evasion of the host immune defenses. Both BmIPA48 and Bm960 are recognized by antibodies in sera from humans infected with B. microti and might be promising candidates for developing novel serodiagnosis and vaccines.
Instituto de Patobiología
Fil: Bastos, Reginaldo. Washington State University. College of Veterinary Medicine. Department of Veterinary Microbiology and Pathology; Estados Unidos
Fil: Thekkiniath, Jose. Fuller Laboratories; Estados Unidos
Fil: Ben Mamoun, Choukri. Yale School of Medicine. Department of Internal Medicine. Section of Infectious Diseases; Estados Unidos
Fil: Fuller, Lee. Fuller Laboratories; Estados Unidos
Fil: Molestina, Robert E. American Type Culture Collection. Protistology Laboratory; Estados Unidos
Fil: Florin-Christensen, Mónica. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Florin-Christensen, Mónica. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología Veterinaria; Argentina
Fil: Schnittger, Leonhard. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Schnittger, Leonhard. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología Veterinaria; Argentina
Fil: Alzan, Heba F. Washington State University. College of Veterinary Medicine. Department of Veterinary Microbiology and Pathology; Estados Unidos
Fil: Alzan, Heba F. National Research Center. Parasitology and Animal Diseases Department; Egipto
Fil: Alzan, Heba F. National Research Center. Tick and Tick-Borne Disease Research Unit; Egipto
Fil: Suarez, Carlos. Washington State University. College of Veterinary Medicine. Department of Veterinary Microbiology and Pathology; Estados Unidos
Fil: Suarez, Carlos. United States Department of Agricultural—Agricultural Research Service. Animal Disease Research Unit; Estados Unidos
Fuente
Pathogens 10 (11) : 1384 (Noviembre 2021)
Materia
Babesia microti
Piroplasmosis
Human Babesiosis
Babesiosis Humana
Nivel de accesibilidad
acceso abierto
Condiciones de uso
http://creativecommons.org/licenses/by-nc-sa/4.0/
Repositorio
INTA Digital (INTA)
Institución
Instituto Nacional de Tecnología Agropecuaria
OAI Identificador
oai:localhost:20.500.12123/16604
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oai_identifier_str oai:localhost:20.500.12123/16604
network_acronym_str INTADig
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network_name_str INTA Digital (INTA)
spelling Babesia microti immunoreactive rhoptry-associated protein-1 paralogs are ancestral members of the piroplasmid-confined RAP-1 familyBastos, ReginaldoThekkiniath, JoseBen Mamoun, ChoukriFuller, LeeMolestina, Robert E.Florin-Christensen, MónicaSchnittger, LeonhardAlzan, Heba F.Suarez, CarlosBabesia microtiPiroplasmosisHuman BabesiosisBabesiosis HumanaBabesia, Cytauxzoon and Theileria are tick-borne apicomplexan parasites of the order Piroplasmida, responsible for diseases in humans and animals. Members of the piroplasmid rhoptry-associated protein-1 (pRAP-1) family have a signature cysteine-rich domain and are important for parasite development. We propose that the closely linked B. microti genes annotated as BMR1_03g00947 and BMR1_03g00960 encode two paralogue pRAP-1-like proteins named BmIPA48 and Bm960. The two genes are tandemly arranged head to tail, highly expressed in blood stage parasites, syntenic to rap-1 genes of other piroplasmids, and share large portions of an almost identical ~225 bp sequence located in their 5′ putative regulatory regions. BmIPA48 and Bm960 proteins contain a N-terminal signal peptide, share very low sequence identity (<13%) with pRAP-1 from other species, and harbor one or more transmembrane domains. Diversification of the piroplasmid-confined prap-1 family is characterized by amplification of genes, protein domains, and a high sequence polymorphism. This suggests a functional involvement of pRAP-1 at the parasite-host interface, possibly in parasite adhesion, attachment, and/or evasion of the host immune defenses. Both BmIPA48 and Bm960 are recognized by antibodies in sera from humans infected with B. microti and might be promising candidates for developing novel serodiagnosis and vaccines.Instituto de PatobiologíaFil: Bastos, Reginaldo. Washington State University. College of Veterinary Medicine. Department of Veterinary Microbiology and Pathology; Estados UnidosFil: Thekkiniath, Jose. Fuller Laboratories; Estados UnidosFil: Ben Mamoun, Choukri. Yale School of Medicine. Department of Internal Medicine. Section of Infectious Diseases; Estados UnidosFil: Fuller, Lee. Fuller Laboratories; Estados UnidosFil: Molestina, Robert E. American Type Culture Collection. Protistology Laboratory; Estados UnidosFil: Florin-Christensen, Mónica. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Florin-Christensen, Mónica. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología Veterinaria; ArgentinaFil: Schnittger, Leonhard. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Schnittger, Leonhard. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología Veterinaria; ArgentinaFil: Alzan, Heba F. Washington State University. College of Veterinary Medicine. Department of Veterinary Microbiology and Pathology; Estados UnidosFil: Alzan, Heba F. National Research Center. Parasitology and Animal Diseases Department; EgiptoFil: Alzan, Heba F. National Research Center. Tick and Tick-Borne Disease Research Unit; EgiptoFil: Suarez, Carlos. Washington State University. College of Veterinary Medicine. Department of Veterinary Microbiology and Pathology; Estados UnidosFil: Suarez, Carlos. United States Department of Agricultural—Agricultural Research Service. Animal Disease Research Unit; Estados UnidosMDPI2024-02-14T17:07:34Z2024-02-14T17:07:34Z2021-11info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://hdl.handle.net/20.500.12123/16604https://www.mdpi.com/2076-0817/10/11/13842076-0817https://doi.org/10.3390/pathogens10111384Pathogens 10 (11) : 1384 (Noviembre 2021)reponame:INTA Digital (INTA)instname:Instituto Nacional de Tecnología Agropecuariaenginfo:eu-repograntAgreement/INTA/2019-PD-E5-I102-001, Desarrollo de vacunas y tecnologías para mejorar las estrategias profilácticas y terapéuticas de las enfermedades que afectan la producción animal y la salud públicainfo:eu-repograntAgreement/INTA/2019-PE-E5-I109-001, Convocatoria: Estudios para el control de enfermedades subtropicales y/o transmitidas por vectores (Tristeza Bovina, Garrapatas, Miasis, Tripanosomiasis, Lengua Azul y lainfo:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by-nc-sa/4.0/Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)2025-09-04T09:50:11Zoai:localhost:20.500.12123/16604instacron:INTAInstitucionalhttp://repositorio.inta.gob.ar/Organismo científico-tecnológicoNo correspondehttp://repositorio.inta.gob.ar/oai/requesttripaldi.nicolas@inta.gob.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:l2025-09-04 09:50:11.946INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuariafalse
dc.title.none.fl_str_mv Babesia microti immunoreactive rhoptry-associated protein-1 paralogs are ancestral members of the piroplasmid-confined RAP-1 family
title Babesia microti immunoreactive rhoptry-associated protein-1 paralogs are ancestral members of the piroplasmid-confined RAP-1 family
spellingShingle Babesia microti immunoreactive rhoptry-associated protein-1 paralogs are ancestral members of the piroplasmid-confined RAP-1 family
Bastos, Reginaldo
Babesia microti
Piroplasmosis
Human Babesiosis
Babesiosis Humana
title_short Babesia microti immunoreactive rhoptry-associated protein-1 paralogs are ancestral members of the piroplasmid-confined RAP-1 family
title_full Babesia microti immunoreactive rhoptry-associated protein-1 paralogs are ancestral members of the piroplasmid-confined RAP-1 family
title_fullStr Babesia microti immunoreactive rhoptry-associated protein-1 paralogs are ancestral members of the piroplasmid-confined RAP-1 family
title_full_unstemmed Babesia microti immunoreactive rhoptry-associated protein-1 paralogs are ancestral members of the piroplasmid-confined RAP-1 family
title_sort Babesia microti immunoreactive rhoptry-associated protein-1 paralogs are ancestral members of the piroplasmid-confined RAP-1 family
dc.creator.none.fl_str_mv Bastos, Reginaldo
Thekkiniath, Jose
Ben Mamoun, Choukri
Fuller, Lee
Molestina, Robert E.
Florin-Christensen, Mónica
Schnittger, Leonhard
Alzan, Heba F.
Suarez, Carlos
author Bastos, Reginaldo
author_facet Bastos, Reginaldo
Thekkiniath, Jose
Ben Mamoun, Choukri
Fuller, Lee
Molestina, Robert E.
Florin-Christensen, Mónica
Schnittger, Leonhard
Alzan, Heba F.
Suarez, Carlos
author_role author
author2 Thekkiniath, Jose
Ben Mamoun, Choukri
Fuller, Lee
Molestina, Robert E.
Florin-Christensen, Mónica
Schnittger, Leonhard
Alzan, Heba F.
Suarez, Carlos
author2_role author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Babesia microti
Piroplasmosis
Human Babesiosis
Babesiosis Humana
topic Babesia microti
Piroplasmosis
Human Babesiosis
Babesiosis Humana
dc.description.none.fl_txt_mv Babesia, Cytauxzoon and Theileria are tick-borne apicomplexan parasites of the order Piroplasmida, responsible for diseases in humans and animals. Members of the piroplasmid rhoptry-associated protein-1 (pRAP-1) family have a signature cysteine-rich domain and are important for parasite development. We propose that the closely linked B. microti genes annotated as BMR1_03g00947 and BMR1_03g00960 encode two paralogue pRAP-1-like proteins named BmIPA48 and Bm960. The two genes are tandemly arranged head to tail, highly expressed in blood stage parasites, syntenic to rap-1 genes of other piroplasmids, and share large portions of an almost identical ~225 bp sequence located in their 5′ putative regulatory regions. BmIPA48 and Bm960 proteins contain a N-terminal signal peptide, share very low sequence identity (<13%) with pRAP-1 from other species, and harbor one or more transmembrane domains. Diversification of the piroplasmid-confined prap-1 family is characterized by amplification of genes, protein domains, and a high sequence polymorphism. This suggests a functional involvement of pRAP-1 at the parasite-host interface, possibly in parasite adhesion, attachment, and/or evasion of the host immune defenses. Both BmIPA48 and Bm960 are recognized by antibodies in sera from humans infected with B. microti and might be promising candidates for developing novel serodiagnosis and vaccines.
Instituto de Patobiología
Fil: Bastos, Reginaldo. Washington State University. College of Veterinary Medicine. Department of Veterinary Microbiology and Pathology; Estados Unidos
Fil: Thekkiniath, Jose. Fuller Laboratories; Estados Unidos
Fil: Ben Mamoun, Choukri. Yale School of Medicine. Department of Internal Medicine. Section of Infectious Diseases; Estados Unidos
Fil: Fuller, Lee. Fuller Laboratories; Estados Unidos
Fil: Molestina, Robert E. American Type Culture Collection. Protistology Laboratory; Estados Unidos
Fil: Florin-Christensen, Mónica. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Florin-Christensen, Mónica. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología Veterinaria; Argentina
Fil: Schnittger, Leonhard. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Schnittger, Leonhard. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología Veterinaria; Argentina
Fil: Alzan, Heba F. Washington State University. College of Veterinary Medicine. Department of Veterinary Microbiology and Pathology; Estados Unidos
Fil: Alzan, Heba F. National Research Center. Parasitology and Animal Diseases Department; Egipto
Fil: Alzan, Heba F. National Research Center. Tick and Tick-Borne Disease Research Unit; Egipto
Fil: Suarez, Carlos. Washington State University. College of Veterinary Medicine. Department of Veterinary Microbiology and Pathology; Estados Unidos
Fil: Suarez, Carlos. United States Department of Agricultural—Agricultural Research Service. Animal Disease Research Unit; Estados Unidos
description Babesia, Cytauxzoon and Theileria are tick-borne apicomplexan parasites of the order Piroplasmida, responsible for diseases in humans and animals. Members of the piroplasmid rhoptry-associated protein-1 (pRAP-1) family have a signature cysteine-rich domain and are important for parasite development. We propose that the closely linked B. microti genes annotated as BMR1_03g00947 and BMR1_03g00960 encode two paralogue pRAP-1-like proteins named BmIPA48 and Bm960. The two genes are tandemly arranged head to tail, highly expressed in blood stage parasites, syntenic to rap-1 genes of other piroplasmids, and share large portions of an almost identical ~225 bp sequence located in their 5′ putative regulatory regions. BmIPA48 and Bm960 proteins contain a N-terminal signal peptide, share very low sequence identity (<13%) with pRAP-1 from other species, and harbor one or more transmembrane domains. Diversification of the piroplasmid-confined prap-1 family is characterized by amplification of genes, protein domains, and a high sequence polymorphism. This suggests a functional involvement of pRAP-1 at the parasite-host interface, possibly in parasite adhesion, attachment, and/or evasion of the host immune defenses. Both BmIPA48 and Bm960 are recognized by antibodies in sera from humans infected with B. microti and might be promising candidates for developing novel serodiagnosis and vaccines.
publishDate 2021
dc.date.none.fl_str_mv 2021-11
2024-02-14T17:07:34Z
2024-02-14T17:07:34Z
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/20.500.12123/16604
https://www.mdpi.com/2076-0817/10/11/1384
2076-0817
https://doi.org/10.3390/pathogens10111384
url http://hdl.handle.net/20.500.12123/16604
https://www.mdpi.com/2076-0817/10/11/1384
https://doi.org/10.3390/pathogens10111384
identifier_str_mv 2076-0817
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repograntAgreement/INTA/2019-PD-E5-I102-001, Desarrollo de vacunas y tecnologías para mejorar las estrategias profilácticas y terapéuticas de las enfermedades que afectan la producción animal y la salud pública
info:eu-repograntAgreement/INTA/2019-PE-E5-I109-001, Convocatoria: Estudios para el control de enfermedades subtropicales y/o transmitidas por vectores (Tristeza Bovina, Garrapatas, Miasis, Tripanosomiasis, Lengua Azul y la
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
eu_rights_str_mv openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv MDPI
publisher.none.fl_str_mv MDPI
dc.source.none.fl_str_mv Pathogens 10 (11) : 1384 (Noviembre 2021)
reponame:INTA Digital (INTA)
instname:Instituto Nacional de Tecnología Agropecuaria
reponame_str INTA Digital (INTA)
collection INTA Digital (INTA)
instname_str Instituto Nacional de Tecnología Agropecuaria
repository.name.fl_str_mv INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuaria
repository.mail.fl_str_mv tripaldi.nicolas@inta.gob.ar
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