Nitrogen enhances diversity of arbuscular mycorrhizal fungi while phosphorus drives community composition in maize rhizosphere after 19 years of fertilization

Autores
Gaset, Braian; Rubio, Gerardo; Ferrari, Manuel Carlos; Cavigliasso, Pablo; Nishinakamasu, Veronica; Puebla, Andrea Fabiana; Faggioli, Valeria Soledad
Año de publicación
2025
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Background and aims: Nitrogen (N) and phosphorus (P) fertilisers are widely used in agro-ecosystems but can endanger the diversity of beneficial soil-borne biota. This study aimed to determine the impact of long-term N and P fertilisation on arbuscular mycorrhizal fungi (AMF), a group of symbiotic soil fungi, by distinguishing between the effects of individual and combined nutrient applications. Methods: We examined the impacts of long-term (i.e. 19 years) N and P fertilisation at two developmental crop stages: V6 (six fully expanded leaves) and R1 (initiation of flowering, after N addition). We measured mycorrhizal colonisation to test the plant-AMF relationship and used Illumina MiSeq sequencing of 18S rRNA gene from rhizospheric soil to evaluate AMF diversity. Results: N and P fertilisation resulted primarily in additive effects rather than interactive effects. N fertilisation greatly increased alpha diversity (e.g. + 38% Chao2 at R1 sampling time) and changed AMF community composition (presence-absence data at R1). P fertilisation reduced mycorrhizal colonisation (~—8% at V6,—21% at R1), changed community composition (relative abundance data at V6 and R1) and negatively affected the abundance and richness of the predominant family Glomeraceae. Conclusions: Long-term additions of N and P change AMF communities in distinct ways. While N mainly contributes to increases in alpha diversity, P influences the assembly of AMF by altering the dominance of major taxa within the community. Future studies are needed to disentangle the implications of these changes for crop yields and nutrient use efficiency to ensure the sustainability of agro-ecosystems.
EEA Pergamino
Fil: Gaset, Braian. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Pergamino. Manejo de cultivos; Argentina
Fil: Rubio, Gerardo. Universidad de Buenos Aires. Facultad de Agronomía. Fertilidad de Suelos y Fertilizantes. Instituto de Investigaciones en Biociencias Agropecuarias y Ambientales; Argentina
Fil: Ferrari, Manuel Carlos. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Pergamino. Producción Vegetal, Mejoramiento y Gestión Ambiental; Argentina
Fil: Cavigliasso, Pablo. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Marcos Juárez; Argentina
Fil: Nishinakamasu, Verónica. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina
Fil: Nishinakamasu, Verónica. Instituto Nacional de Tecnología Agropecuaria (INTA). Centro de Investigación en Ciencias Veterinarias y Agronómicas; Argentina
Fil: Nishinakamasu, Verónica. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Nishinakamasu, Verónica. Unidad de Genómica y Bioinformática; Argentina
Fil: Puebla, Andrea. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina
Fil: Puebla, Andrea. Instituto Nacional de Tecnología Agropecuaria (INTA). Centro de Investigación en Ciencias Veterinarias y Agronómicas; Argentina
Fil: Puebla, Andrea. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Puebla, Andrea. Unidad de Genómica y Bioinformática; Argentina
Fil: Faggioli, Valeria Soledad. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Marcos Juárez; Argentina
Fuente
Plant and Soil 506 (1-2) : 1-18. (January 2025)
Materia
Maíz
Nitrógeno
Fósforo
Agroecosistemas
Sostenibilidad
Argentina
Maize
Nitrogen
Phosphorus
Agroecosystems
Sustainability
Arbuscular Mycorrhiza
Micorrizas Arbusculares
Fertilización
No-tillage
Nivel de accesibilidad
acceso restringido
Condiciones de uso
http://creativecommons.org/licenses/by-nc-sa/4.0/
Repositorio
INTA Digital (INTA)
Institución
Instituto Nacional de Tecnología Agropecuaria
OAI Identificador
oai:localhost:20.500.12123/21601
Acceder
id INTADig_5985f2ee8c5bd90123f4b9b80cd8f534
oai_identifier_str oai:localhost:20.500.12123/21601
network_acronym_str INTADig
repository_id_str l
network_name_str INTA Digital (INTA)
spelling Nitrogen enhances diversity of arbuscular mycorrhizal fungi while phosphorus drives community composition in maize rhizosphere after 19 years of fertilizationGaset, BraianRubio, GerardoFerrari, Manuel CarlosCavigliasso, PabloNishinakamasu, VeronicaPuebla, Andrea FabianaFaggioli, Valeria SoledadMaízNitrógenoFósforoAgroecosistemasSostenibilidadArgentinaMaizeNitrogenPhosphorusAgroecosystemsSustainabilityArbuscular MycorrhizaMicorrizas ArbuscularesFertilizaciónNo-tillageBackground and aims: Nitrogen (N) and phosphorus (P) fertilisers are widely used in agro-ecosystems but can endanger the diversity of beneficial soil-borne biota. This study aimed to determine the impact of long-term N and P fertilisation on arbuscular mycorrhizal fungi (AMF), a group of symbiotic soil fungi, by distinguishing between the effects of individual and combined nutrient applications. Methods: We examined the impacts of long-term (i.e. 19 years) N and P fertilisation at two developmental crop stages: V6 (six fully expanded leaves) and R1 (initiation of flowering, after N addition). We measured mycorrhizal colonisation to test the plant-AMF relationship and used Illumina MiSeq sequencing of 18S rRNA gene from rhizospheric soil to evaluate AMF diversity. Results: N and P fertilisation resulted primarily in additive effects rather than interactive effects. N fertilisation greatly increased alpha diversity (e.g. + 38% Chao2 at R1 sampling time) and changed AMF community composition (presence-absence data at R1). P fertilisation reduced mycorrhizal colonisation (~—8% at V6,—21% at R1), changed community composition (relative abundance data at V6 and R1) and negatively affected the abundance and richness of the predominant family Glomeraceae. Conclusions: Long-term additions of N and P change AMF communities in distinct ways. While N mainly contributes to increases in alpha diversity, P influences the assembly of AMF by altering the dominance of major taxa within the community. Future studies are needed to disentangle the implications of these changes for crop yields and nutrient use efficiency to ensure the sustainability of agro-ecosystems.EEA PergaminoFil: Gaset, Braian. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Pergamino. Manejo de cultivos; ArgentinaFil: Rubio, Gerardo. Universidad de Buenos Aires. Facultad de Agronomía. Fertilidad de Suelos y Fertilizantes. Instituto de Investigaciones en Biociencias Agropecuarias y Ambientales; ArgentinaFil: Ferrari, Manuel Carlos. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Pergamino. Producción Vegetal, Mejoramiento y Gestión Ambiental; ArgentinaFil: Cavigliasso, Pablo. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Marcos Juárez; ArgentinaFil: Nishinakamasu, Verónica. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; ArgentinaFil: Nishinakamasu, Verónica. Instituto Nacional de Tecnología Agropecuaria (INTA). Centro de Investigación en Ciencias Veterinarias y Agronómicas; ArgentinaFil: Nishinakamasu, Verónica. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Nishinakamasu, Verónica. Unidad de Genómica y Bioinformática; ArgentinaFil: Puebla, Andrea. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; ArgentinaFil: Puebla, Andrea. Instituto Nacional de Tecnología Agropecuaria (INTA). Centro de Investigación en Ciencias Veterinarias y Agronómicas; ArgentinaFil: Puebla, Andrea. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Puebla, Andrea. Unidad de Genómica y Bioinformática; ArgentinaFil: Faggioli, Valeria Soledad. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Marcos Juárez; ArgentinaSpringer Nature2025-03-10T10:27:14Z2025-03-10T10:27:14Z2025-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://hdl.handle.net/20.500.12123/21601https://link.springer.com/article/10.1007/s11104-024-07186-50032-079X1573-5036 (online)https://doi.org/10.1007/s11104-024-07186-5Plant and Soil 506 (1-2) : 1-18. (January 2025)reponame:INTA Digital (INTA)instname:Instituto Nacional de Tecnología Agropecuariaenginfo:eu-repograntAgreement/INTA/2023-PD-L03-I093, Fertilidad química, física y biológica del suelo: estrategias para mitigar y restaurar procesos de degradacióninfo:eu-repograntAgreement/INTA/2019-PD-E2-I037-002, Biodiversidad edáfica: componente clave para una gestión integral y sustentable del recurso sueloinfo:eu-repograntAgreement/INTA/2019-PE-E1-I011-001, Intensificacion Sustentable de la Agricultura en la Region Pampeanainfo:eu-repograntAgreement/INTA/2019-RIST-E1-I503-001, Red de ensayos de larga duracióninfo:eu-repo/semantics/restrictedAccesshttp://creativecommons.org/licenses/by-nc-sa/4.0/Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)2025-10-16T09:32:11Zoai:localhost:20.500.12123/21601instacron:INTAInstitucionalhttp://repositorio.inta.gob.ar/Organismo científico-tecnológicoNo correspondehttp://repositorio.inta.gob.ar/oai/requesttripaldi.nicolas@inta.gob.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:l2025-10-16 09:32:12.163INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuariafalse
dc.title.none.fl_str_mv Nitrogen enhances diversity of arbuscular mycorrhizal fungi while phosphorus drives community composition in maize rhizosphere after 19 years of fertilization
title Nitrogen enhances diversity of arbuscular mycorrhizal fungi while phosphorus drives community composition in maize rhizosphere after 19 years of fertilization
spellingShingle Nitrogen enhances diversity of arbuscular mycorrhizal fungi while phosphorus drives community composition in maize rhizosphere after 19 years of fertilization
Gaset, Braian
Maíz
Nitrógeno
Fósforo
Agroecosistemas
Sostenibilidad
Argentina
Maize
Nitrogen
Phosphorus
Agroecosystems
Sustainability
Arbuscular Mycorrhiza
Micorrizas Arbusculares
Fertilización
No-tillage
title_short Nitrogen enhances diversity of arbuscular mycorrhizal fungi while phosphorus drives community composition in maize rhizosphere after 19 years of fertilization
title_full Nitrogen enhances diversity of arbuscular mycorrhizal fungi while phosphorus drives community composition in maize rhizosphere after 19 years of fertilization
title_fullStr Nitrogen enhances diversity of arbuscular mycorrhizal fungi while phosphorus drives community composition in maize rhizosphere after 19 years of fertilization
title_full_unstemmed Nitrogen enhances diversity of arbuscular mycorrhizal fungi while phosphorus drives community composition in maize rhizosphere after 19 years of fertilization
title_sort Nitrogen enhances diversity of arbuscular mycorrhizal fungi while phosphorus drives community composition in maize rhizosphere after 19 years of fertilization
dc.creator.none.fl_str_mv Gaset, Braian
Rubio, Gerardo
Ferrari, Manuel Carlos
Cavigliasso, Pablo
Nishinakamasu, Veronica
Puebla, Andrea Fabiana
Faggioli, Valeria Soledad
author Gaset, Braian
author_facet Gaset, Braian
Rubio, Gerardo
Ferrari, Manuel Carlos
Cavigliasso, Pablo
Nishinakamasu, Veronica
Puebla, Andrea Fabiana
Faggioli, Valeria Soledad
author_role author
author2 Rubio, Gerardo
Ferrari, Manuel Carlos
Cavigliasso, Pablo
Nishinakamasu, Veronica
Puebla, Andrea Fabiana
Faggioli, Valeria Soledad
author2_role author
author
author
author
author
author
dc.subject.none.fl_str_mv Maíz
Nitrógeno
Fósforo
Agroecosistemas
Sostenibilidad
Argentina
Maize
Nitrogen
Phosphorus
Agroecosystems
Sustainability
Arbuscular Mycorrhiza
Micorrizas Arbusculares
Fertilización
No-tillage
topic Maíz
Nitrógeno
Fósforo
Agroecosistemas
Sostenibilidad
Argentina
Maize
Nitrogen
Phosphorus
Agroecosystems
Sustainability
Arbuscular Mycorrhiza
Micorrizas Arbusculares
Fertilización
No-tillage
dc.description.none.fl_txt_mv Background and aims: Nitrogen (N) and phosphorus (P) fertilisers are widely used in agro-ecosystems but can endanger the diversity of beneficial soil-borne biota. This study aimed to determine the impact of long-term N and P fertilisation on arbuscular mycorrhizal fungi (AMF), a group of symbiotic soil fungi, by distinguishing between the effects of individual and combined nutrient applications. Methods: We examined the impacts of long-term (i.e. 19 years) N and P fertilisation at two developmental crop stages: V6 (six fully expanded leaves) and R1 (initiation of flowering, after N addition). We measured mycorrhizal colonisation to test the plant-AMF relationship and used Illumina MiSeq sequencing of 18S rRNA gene from rhizospheric soil to evaluate AMF diversity. Results: N and P fertilisation resulted primarily in additive effects rather than interactive effects. N fertilisation greatly increased alpha diversity (e.g. + 38% Chao2 at R1 sampling time) and changed AMF community composition (presence-absence data at R1). P fertilisation reduced mycorrhizal colonisation (~—8% at V6,—21% at R1), changed community composition (relative abundance data at V6 and R1) and negatively affected the abundance and richness of the predominant family Glomeraceae. Conclusions: Long-term additions of N and P change AMF communities in distinct ways. While N mainly contributes to increases in alpha diversity, P influences the assembly of AMF by altering the dominance of major taxa within the community. Future studies are needed to disentangle the implications of these changes for crop yields and nutrient use efficiency to ensure the sustainability of agro-ecosystems.
EEA Pergamino
Fil: Gaset, Braian. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Pergamino. Manejo de cultivos; Argentina
Fil: Rubio, Gerardo. Universidad de Buenos Aires. Facultad de Agronomía. Fertilidad de Suelos y Fertilizantes. Instituto de Investigaciones en Biociencias Agropecuarias y Ambientales; Argentina
Fil: Ferrari, Manuel Carlos. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Pergamino. Producción Vegetal, Mejoramiento y Gestión Ambiental; Argentina
Fil: Cavigliasso, Pablo. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Marcos Juárez; Argentina
Fil: Nishinakamasu, Verónica. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina
Fil: Nishinakamasu, Verónica. Instituto Nacional de Tecnología Agropecuaria (INTA). Centro de Investigación en Ciencias Veterinarias y Agronómicas; Argentina
Fil: Nishinakamasu, Verónica. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Nishinakamasu, Verónica. Unidad de Genómica y Bioinformática; Argentina
Fil: Puebla, Andrea. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina
Fil: Puebla, Andrea. Instituto Nacional de Tecnología Agropecuaria (INTA). Centro de Investigación en Ciencias Veterinarias y Agronómicas; Argentina
Fil: Puebla, Andrea. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Puebla, Andrea. Unidad de Genómica y Bioinformática; Argentina
Fil: Faggioli, Valeria Soledad. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Marcos Juárez; Argentina
description Background and aims: Nitrogen (N) and phosphorus (P) fertilisers are widely used in agro-ecosystems but can endanger the diversity of beneficial soil-borne biota. This study aimed to determine the impact of long-term N and P fertilisation on arbuscular mycorrhizal fungi (AMF), a group of symbiotic soil fungi, by distinguishing between the effects of individual and combined nutrient applications. Methods: We examined the impacts of long-term (i.e. 19 years) N and P fertilisation at two developmental crop stages: V6 (six fully expanded leaves) and R1 (initiation of flowering, after N addition). We measured mycorrhizal colonisation to test the plant-AMF relationship and used Illumina MiSeq sequencing of 18S rRNA gene from rhizospheric soil to evaluate AMF diversity. Results: N and P fertilisation resulted primarily in additive effects rather than interactive effects. N fertilisation greatly increased alpha diversity (e.g. + 38% Chao2 at R1 sampling time) and changed AMF community composition (presence-absence data at R1). P fertilisation reduced mycorrhizal colonisation (~—8% at V6,—21% at R1), changed community composition (relative abundance data at V6 and R1) and negatively affected the abundance and richness of the predominant family Glomeraceae. Conclusions: Long-term additions of N and P change AMF communities in distinct ways. While N mainly contributes to increases in alpha diversity, P influences the assembly of AMF by altering the dominance of major taxa within the community. Future studies are needed to disentangle the implications of these changes for crop yields and nutrient use efficiency to ensure the sustainability of agro-ecosystems.
publishDate 2025
dc.date.none.fl_str_mv 2025-03-10T10:27:14Z
2025-03-10T10:27:14Z
2025-01
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/20.500.12123/21601
https://link.springer.com/article/10.1007/s11104-024-07186-5
0032-079X
1573-5036 (online)
https://doi.org/10.1007/s11104-024-07186-5
url http://hdl.handle.net/20.500.12123/21601
https://link.springer.com/article/10.1007/s11104-024-07186-5
https://doi.org/10.1007/s11104-024-07186-5
identifier_str_mv 0032-079X
1573-5036 (online)
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repograntAgreement/INTA/2023-PD-L03-I093, Fertilidad química, física y biológica del suelo: estrategias para mitigar y restaurar procesos de degradación
info:eu-repograntAgreement/INTA/2019-PD-E2-I037-002, Biodiversidad edáfica: componente clave para una gestión integral y sustentable del recurso suelo
info:eu-repograntAgreement/INTA/2019-PE-E1-I011-001, Intensificacion Sustentable de la Agricultura en la Region Pampeana
info:eu-repograntAgreement/INTA/2019-RIST-E1-I503-001, Red de ensayos de larga duración
dc.rights.none.fl_str_mv info:eu-repo/semantics/restrictedAccess
http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
eu_rights_str_mv restrictedAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Springer Nature
publisher.none.fl_str_mv Springer Nature
dc.source.none.fl_str_mv Plant and Soil 506 (1-2) : 1-18. (January 2025)
reponame:INTA Digital (INTA)
instname:Instituto Nacional de Tecnología Agropecuaria
reponame_str INTA Digital (INTA)
collection INTA Digital (INTA)
instname_str Instituto Nacional de Tecnología Agropecuaria
repository.name.fl_str_mv INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuaria
repository.mail.fl_str_mv tripaldi.nicolas@inta.gob.ar
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