Natural occurrence of Azospirillum brasilense in petunia with capacity to improve plant growth and flowering

Autores
Toffoli, Lucia Mercedes; Martinez Zamora, Martin Gustavo; Medrano, Norma Nelly; Fontana, Cecilia Alejandra; Lovaisa, Nadia Carolina; Delaporte Quintana, Paola Adriana Georgina; Elias, Juliana M.; Salazar, Sergio Miguel; Pedraza, Raúl Osvaldo
Año de publicación
2021
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
To evaluate the natural occurrence of the plant growth-promoting bacterium Azospirillum brasilense and petunia plants, local strains were isolated and characterized by biochemical and molecular methods. Three strains were assessed in greenhouse conditions using Petunia × hybrida Ultra™. Treatments: Plants without bacterial inoculation or chemical fertilization; fertilized with NPK and KNO3; and independently inoculated with the strains 2A1, 2A2, and 2E1 by submerging their roots in a bacterial suspension (~106 CFU·ml−1). Root length, dry weight of roots and shoots, leaf area, leaf greenness, and nutrient content were evaluated. The number of days from transplanting to the opening of the first flower and the number of flowers per plant were also determined. As a result, five isolates were characterized as A. brasilense, showing the capacity to produce indoles and siderophores, to solubilize phosphate, nitrogenase activity, and nifH-PCR amplification. In general, all the parameters of the plant assay were improved in plants inoculated with A. brasilense, with variations among the strains, as well as the onset of flowering and the number of flowers per plant, compared with uninoculated or fertilized plants. This is the first report on the natural occurrence of A. brasilense in petunia with the capacity to improve plant growth and flowering.
EEA Famaillá
Fil: Toffoli, Lucia Mercedes. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Famaillá; Argentina
Fil: Martinez Zamora, Martin Gustavo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto Superior de Investigaciones Biológicas; Argentina
Fil: Martinez Zamora, Martin Gustavo. Universidad Nacional de Tucumán. Instituto Superior de Investigaciones Biológicas; Argentina
Fil: Medrano, Norma Nelly. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Famaillá; Argentina
Fil: Fontana, Cecilia Alejandra. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Famaillá; Argentina
Fil: Lovaisa, Nadia Carolina. Universidad Nacional de Tucumán. Facultad de Agronomía y Zootecnia; Argentina.
Fil: Delaporte Quintana, Paola Adriana Georgina. Universidad Nacional de Tucumán. Facultad de Agronomía y Zootecnia; Argentina
Fil: Elías, J. Universidad Nacional de Tucumán. Facultad de Agronomía y Zootecnia; Argentina
Fil: Salazar, Sergio Miguel. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Famaillá; Argentina
Fil: Salazar, Sergio Miguel. Universidad Nacional de Tucumán. Facultad de Agronomía y Zootecnia; Argentina
Fil: Pedraza, Raúl Osvaldo. Universidad Nacional de Tucumán. Facultad de Agronomía y Zootecnia; Argentina
Fuente
Journal of Basic Microbiology 61 (7) : 662-673. (July 2021)
Materia
Petunia
Floración
Azospirillum brasilense
Sideróforos
Índoles
Flowering
Siderophores
Indoles
Plant Growth Substances
Sustancias de Crecimiento Vegetal
Nivel de accesibilidad
acceso restringido
Condiciones de uso
Repositorio
INTA Digital (INTA)
Institución
Instituto Nacional de Tecnología Agropecuaria
OAI Identificador
oai:localhost:20.500.12123/9489
Acceder
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oai_identifier_str oai:localhost:20.500.12123/9489
network_acronym_str INTADig
repository_id_str l
network_name_str INTA Digital (INTA)
spelling Natural occurrence of Azospirillum brasilense in petunia with capacity to improve plant growth and floweringToffoli, Lucia MercedesMartinez Zamora, Martin GustavoMedrano, Norma NellyFontana, Cecilia AlejandraLovaisa, Nadia CarolinaDelaporte Quintana, Paola Adriana GeorginaElias, Juliana M.Salazar, Sergio MiguelPedraza, Raúl OsvaldoPetuniaFloraciónAzospirillum brasilenseSideróforosÍndolesFloweringSiderophoresIndolesPlant Growth SubstancesSustancias de Crecimiento VegetalTo evaluate the natural occurrence of the plant growth-promoting bacterium Azospirillum brasilense and petunia plants, local strains were isolated and characterized by biochemical and molecular methods. Three strains were assessed in greenhouse conditions using Petunia × hybrida Ultra™. Treatments: Plants without bacterial inoculation or chemical fertilization; fertilized with NPK and KNO3; and independently inoculated with the strains 2A1, 2A2, and 2E1 by submerging their roots in a bacterial suspension (~106 CFU·ml−1). Root length, dry weight of roots and shoots, leaf area, leaf greenness, and nutrient content were evaluated. The number of days from transplanting to the opening of the first flower and the number of flowers per plant were also determined. As a result, five isolates were characterized as A. brasilense, showing the capacity to produce indoles and siderophores, to solubilize phosphate, nitrogenase activity, and nifH-PCR amplification. In general, all the parameters of the plant assay were improved in plants inoculated with A. brasilense, with variations among the strains, as well as the onset of flowering and the number of flowers per plant, compared with uninoculated or fertilized plants. This is the first report on the natural occurrence of A. brasilense in petunia with the capacity to improve plant growth and flowering.EEA FamailláFil: Toffoli, Lucia Mercedes. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Famaillá; ArgentinaFil: Martinez Zamora, Martin Gustavo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto Superior de Investigaciones Biológicas; ArgentinaFil: Martinez Zamora, Martin Gustavo. Universidad Nacional de Tucumán. Instituto Superior de Investigaciones Biológicas; ArgentinaFil: Medrano, Norma Nelly. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Famaillá; ArgentinaFil: Fontana, Cecilia Alejandra. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Famaillá; ArgentinaFil: Lovaisa, Nadia Carolina. Universidad Nacional de Tucumán. Facultad de Agronomía y Zootecnia; Argentina.Fil: Delaporte Quintana, Paola Adriana Georgina. Universidad Nacional de Tucumán. Facultad de Agronomía y Zootecnia; ArgentinaFil: Elías, J. Universidad Nacional de Tucumán. Facultad de Agronomía y Zootecnia; ArgentinaFil: Salazar, Sergio Miguel. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Famaillá; ArgentinaFil: Salazar, Sergio Miguel. Universidad Nacional de Tucumán. Facultad de Agronomía y Zootecnia; ArgentinaFil: Pedraza, Raúl Osvaldo. Universidad Nacional de Tucumán. Facultad de Agronomía y Zootecnia; ArgentinaWiley2021-06-03T13:34:47Z2021-06-03T13:34:47Z2021-05-31info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://hdl.handle.net/20.500.12123/9489https://onlinelibrary.wiley.com/doi/10.1002/jobm.2021000640233-111X1521-4028https://doi.org/10.1002/jobm.202100064Journal of Basic Microbiology 61 (7) : 662-673. (July 2021)reponame:INTA Digital (INTA)instname:Instituto Nacional de Tecnología Agropecuariaenginfo:eu-repograntAgreement/INTA/PNHFA-1106093/AR./Desarrollo y ajuste de tecnologías para una producción florícola sustentable y de calidad.info:eu-repograntAgreement/INTA/TUSGO-1231101/AR./Desarrollo sustentable de los territorios a traves de procesos de innovación tecnológica, socio organizativa e institucional del área norte de la provincia de Tucumán.info:eu-repo/semantics/restrictedAccess2025-09-04T09:48:55Zoai:localhost:20.500.12123/9489instacron:INTAInstitucionalhttp://repositorio.inta.gob.ar/Organismo científico-tecnológicoNo correspondehttp://repositorio.inta.gob.ar/oai/requesttripaldi.nicolas@inta.gob.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:l2025-09-04 09:48:55.837INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuariafalse
dc.title.none.fl_str_mv Natural occurrence of Azospirillum brasilense in petunia with capacity to improve plant growth and flowering
title Natural occurrence of Azospirillum brasilense in petunia with capacity to improve plant growth and flowering
spellingShingle Natural occurrence of Azospirillum brasilense in petunia with capacity to improve plant growth and flowering
Toffoli, Lucia Mercedes
Petunia
Floración
Azospirillum brasilense
Sideróforos
Índoles
Flowering
Siderophores
Indoles
Plant Growth Substances
Sustancias de Crecimiento Vegetal
title_short Natural occurrence of Azospirillum brasilense in petunia with capacity to improve plant growth and flowering
title_full Natural occurrence of Azospirillum brasilense in petunia with capacity to improve plant growth and flowering
title_fullStr Natural occurrence of Azospirillum brasilense in petunia with capacity to improve plant growth and flowering
title_full_unstemmed Natural occurrence of Azospirillum brasilense in petunia with capacity to improve plant growth and flowering
title_sort Natural occurrence of Azospirillum brasilense in petunia with capacity to improve plant growth and flowering
dc.creator.none.fl_str_mv Toffoli, Lucia Mercedes
Martinez Zamora, Martin Gustavo
Medrano, Norma Nelly
Fontana, Cecilia Alejandra
Lovaisa, Nadia Carolina
Delaporte Quintana, Paola Adriana Georgina
Elias, Juliana M.
Salazar, Sergio Miguel
Pedraza, Raúl Osvaldo
author Toffoli, Lucia Mercedes
author_facet Toffoli, Lucia Mercedes
Martinez Zamora, Martin Gustavo
Medrano, Norma Nelly
Fontana, Cecilia Alejandra
Lovaisa, Nadia Carolina
Delaporte Quintana, Paola Adriana Georgina
Elias, Juliana M.
Salazar, Sergio Miguel
Pedraza, Raúl Osvaldo
author_role author
author2 Martinez Zamora, Martin Gustavo
Medrano, Norma Nelly
Fontana, Cecilia Alejandra
Lovaisa, Nadia Carolina
Delaporte Quintana, Paola Adriana Georgina
Elias, Juliana M.
Salazar, Sergio Miguel
Pedraza, Raúl Osvaldo
author2_role author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Petunia
Floración
Azospirillum brasilense
Sideróforos
Índoles
Flowering
Siderophores
Indoles
Plant Growth Substances
Sustancias de Crecimiento Vegetal
topic Petunia
Floración
Azospirillum brasilense
Sideróforos
Índoles
Flowering
Siderophores
Indoles
Plant Growth Substances
Sustancias de Crecimiento Vegetal
dc.description.none.fl_txt_mv To evaluate the natural occurrence of the plant growth-promoting bacterium Azospirillum brasilense and petunia plants, local strains were isolated and characterized by biochemical and molecular methods. Three strains were assessed in greenhouse conditions using Petunia × hybrida Ultra™. Treatments: Plants without bacterial inoculation or chemical fertilization; fertilized with NPK and KNO3; and independently inoculated with the strains 2A1, 2A2, and 2E1 by submerging their roots in a bacterial suspension (~106 CFU·ml−1). Root length, dry weight of roots and shoots, leaf area, leaf greenness, and nutrient content were evaluated. The number of days from transplanting to the opening of the first flower and the number of flowers per plant were also determined. As a result, five isolates were characterized as A. brasilense, showing the capacity to produce indoles and siderophores, to solubilize phosphate, nitrogenase activity, and nifH-PCR amplification. In general, all the parameters of the plant assay were improved in plants inoculated with A. brasilense, with variations among the strains, as well as the onset of flowering and the number of flowers per plant, compared with uninoculated or fertilized plants. This is the first report on the natural occurrence of A. brasilense in petunia with the capacity to improve plant growth and flowering.
EEA Famaillá
Fil: Toffoli, Lucia Mercedes. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Famaillá; Argentina
Fil: Martinez Zamora, Martin Gustavo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto Superior de Investigaciones Biológicas; Argentina
Fil: Martinez Zamora, Martin Gustavo. Universidad Nacional de Tucumán. Instituto Superior de Investigaciones Biológicas; Argentina
Fil: Medrano, Norma Nelly. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Famaillá; Argentina
Fil: Fontana, Cecilia Alejandra. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Famaillá; Argentina
Fil: Lovaisa, Nadia Carolina. Universidad Nacional de Tucumán. Facultad de Agronomía y Zootecnia; Argentina.
Fil: Delaporte Quintana, Paola Adriana Georgina. Universidad Nacional de Tucumán. Facultad de Agronomía y Zootecnia; Argentina
Fil: Elías, J. Universidad Nacional de Tucumán. Facultad de Agronomía y Zootecnia; Argentina
Fil: Salazar, Sergio Miguel. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Famaillá; Argentina
Fil: Salazar, Sergio Miguel. Universidad Nacional de Tucumán. Facultad de Agronomía y Zootecnia; Argentina
Fil: Pedraza, Raúl Osvaldo. Universidad Nacional de Tucumán. Facultad de Agronomía y Zootecnia; Argentina
description To evaluate the natural occurrence of the plant growth-promoting bacterium Azospirillum brasilense and petunia plants, local strains were isolated and characterized by biochemical and molecular methods. Three strains were assessed in greenhouse conditions using Petunia × hybrida Ultra™. Treatments: Plants without bacterial inoculation or chemical fertilization; fertilized with NPK and KNO3; and independently inoculated with the strains 2A1, 2A2, and 2E1 by submerging their roots in a bacterial suspension (~106 CFU·ml−1). Root length, dry weight of roots and shoots, leaf area, leaf greenness, and nutrient content were evaluated. The number of days from transplanting to the opening of the first flower and the number of flowers per plant were also determined. As a result, five isolates were characterized as A. brasilense, showing the capacity to produce indoles and siderophores, to solubilize phosphate, nitrogenase activity, and nifH-PCR amplification. In general, all the parameters of the plant assay were improved in plants inoculated with A. brasilense, with variations among the strains, as well as the onset of flowering and the number of flowers per plant, compared with uninoculated or fertilized plants. This is the first report on the natural occurrence of A. brasilense in petunia with the capacity to improve plant growth and flowering.
publishDate 2021
dc.date.none.fl_str_mv 2021-06-03T13:34:47Z
2021-06-03T13:34:47Z
2021-05-31
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/20.500.12123/9489
https://onlinelibrary.wiley.com/doi/10.1002/jobm.202100064
0233-111X
1521-4028
https://doi.org/10.1002/jobm.202100064
url http://hdl.handle.net/20.500.12123/9489
https://onlinelibrary.wiley.com/doi/10.1002/jobm.202100064
https://doi.org/10.1002/jobm.202100064
identifier_str_mv 0233-111X
1521-4028
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repograntAgreement/INTA/PNHFA-1106093/AR./Desarrollo y ajuste de tecnologías para una producción florícola sustentable y de calidad.
info:eu-repograntAgreement/INTA/TUSGO-1231101/AR./Desarrollo sustentable de los territorios a traves de procesos de innovación tecnológica, socio organizativa e institucional del área norte de la provincia de Tucumán.
dc.rights.none.fl_str_mv info:eu-repo/semantics/restrictedAccess
eu_rights_str_mv restrictedAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Wiley
publisher.none.fl_str_mv Wiley
dc.source.none.fl_str_mv Journal of Basic Microbiology 61 (7) : 662-673. (July 2021)
reponame:INTA Digital (INTA)
instname:Instituto Nacional de Tecnología Agropecuaria
reponame_str INTA Digital (INTA)
collection INTA Digital (INTA)
instname_str Instituto Nacional de Tecnología Agropecuaria
repository.name.fl_str_mv INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuaria
repository.mail.fl_str_mv tripaldi.nicolas@inta.gob.ar
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