Bovine macrophages transcriptome profiling reveals divergent responses to virulent and attenuated Babesia bovis strains

Autores
Valenzano, Magali Nicole; Alvarez, Liliana; Valentini, Beatriz Susana; Wilkowsky, Silvina Elizabeth
Año de publicación
2026
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Babesia bovis is a tick-borne parasite of major economic impact in the livestock industry. Control strategies rely mainly on the use of acaricides and live attenuated vaccines. Comparative genomic analyses have shown no major differences between virulent and attenuated B. bovis strains, suggesting that studies on the host’s differential response may represent a key step toward clarifying the basis of disease severity and vaccine efficacy. In this study, we analyzed by RNA-seq the differential gene expression in bovine monocyte-derived macrophages after phagocytosis of erythrocytes infected with either the virulent B. bovis S2P strain or the attenuated R1A one. The results revealed a common transcriptional core response of several cellular processes largely centered on lymphocyte related functions, cytokine regulation, and adaptive immune signaling. In turn, the two strains elicited contrasting responses in bovine macrophages, where the virulent strain induced the enrichment of lymphocyte- and antiviral-related pathways, and the attenuated strain led to a stronger pro-inflammatory, chemotactic, and extracellular matrix remodeling signatures. Taken together, these data improved our understanding of the early transcriptional events that develop in macrophages in response to the phagocytosis of red blood cells containing contrasting B. bovis strains. This large dataset could be evaluated in further studies to better characterize the pathogenic mechanisms of bovine babesiosis and to identify targets for host-directed therapeutic strategies.
Instituto de Biotecnología
Fil: Valenzano, Magali Nicole. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina
Fil: Valenzano, Magali Nicole. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Alvarez, Liliana. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina
Fil: Alvarez, Liliana. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Valentini, Beatriz Susana. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rafaela; Argentina
Fil: Wilkowsky, Silvina Elizabeth. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina
Fil: Wilkowsky, Silvina Elizabeth. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fuente
Veterinary Parasitology 341 : 110634. (January 2026)
Materia
Enfermedades de los Animales
Ganado Bovino
Macrófogos
Transcriptoma
Animal Diseases
Babesia bovis
Cattle
Macrophages
Transcriptome
Cepa Atenuada
Attenuated Strain
Nivel de accesibilidad
acceso restringido
Condiciones de uso
http://creativecommons.org/licenses/by-nc-sa/4.0/
Repositorio
INTA Digital (INTA)
Institución
Instituto Nacional de Tecnología Agropecuaria
OAI Identificador
oai:localhost:20.500.12123/25221
Acceder
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oai_identifier_str oai:localhost:20.500.12123/25221
network_acronym_str INTADig
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network_name_str INTA Digital (INTA)
spelling Bovine macrophages transcriptome profiling reveals divergent responses to virulent and attenuated Babesia bovis strainsValenzano, Magali NicoleAlvarez, LilianaValentini, Beatriz SusanaWilkowsky, Silvina ElizabethEnfermedades de los AnimalesGanado BovinoMacrófogosTranscriptomaAnimal DiseasesBabesia bovisCattleMacrophagesTranscriptomeCepa AtenuadaAttenuated StrainBabesia bovis is a tick-borne parasite of major economic impact in the livestock industry. Control strategies rely mainly on the use of acaricides and live attenuated vaccines. Comparative genomic analyses have shown no major differences between virulent and attenuated B. bovis strains, suggesting that studies on the host’s differential response may represent a key step toward clarifying the basis of disease severity and vaccine efficacy. In this study, we analyzed by RNA-seq the differential gene expression in bovine monocyte-derived macrophages after phagocytosis of erythrocytes infected with either the virulent B. bovis S2P strain or the attenuated R1A one. The results revealed a common transcriptional core response of several cellular processes largely centered on lymphocyte related functions, cytokine regulation, and adaptive immune signaling. In turn, the two strains elicited contrasting responses in bovine macrophages, where the virulent strain induced the enrichment of lymphocyte- and antiviral-related pathways, and the attenuated strain led to a stronger pro-inflammatory, chemotactic, and extracellular matrix remodeling signatures. Taken together, these data improved our understanding of the early transcriptional events that develop in macrophages in response to the phagocytosis of red blood cells containing contrasting B. bovis strains. This large dataset could be evaluated in further studies to better characterize the pathogenic mechanisms of bovine babesiosis and to identify targets for host-directed therapeutic strategies.Instituto de BiotecnologíaFil: Valenzano, Magali Nicole. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; ArgentinaFil: Valenzano, Magali Nicole. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Alvarez, Liliana. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; ArgentinaFil: Alvarez, Liliana. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Valentini, Beatriz Susana. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rafaela; ArgentinaFil: Wilkowsky, Silvina Elizabeth. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; ArgentinaFil: Wilkowsky, Silvina Elizabeth. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaElsevier2026-02-18T13:49:17Z2026-02-18T13:49:17Z2026-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://hdl.handle.net/20.500.12123/25221https://www.sciencedirect.com/science/article/abs/pii/S03044017250024560304-40171873-2550https://doi.org/10.1016/j.vetpar.2025.110634Veterinary Parasitology 341 : 110634. (January 2026)reponame:INTA Digital (INTA)instname:Instituto Nacional de Tecnología Agropecuariaenginfo:eu-repo/semantics/restrictedAccesshttp://creativecommons.org/licenses/by-nc-sa/4.0/Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)2026-02-26T11:47:42Zoai:localhost:20.500.12123/25221instacron:INTAInstitucionalhttp://repositorio.inta.gob.ar/Organismo científico-tecnológicoNo correspondehttp://repositorio.inta.gob.ar/oai/requesttripaldi.nicolas@inta.gob.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:l2026-02-26 11:47:42.713INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuariafalse
dc.title.none.fl_str_mv Bovine macrophages transcriptome profiling reveals divergent responses to virulent and attenuated Babesia bovis strains
title Bovine macrophages transcriptome profiling reveals divergent responses to virulent and attenuated Babesia bovis strains
spellingShingle Bovine macrophages transcriptome profiling reveals divergent responses to virulent and attenuated Babesia bovis strains
Valenzano, Magali Nicole
Enfermedades de los Animales
Ganado Bovino
Macrófogos
Transcriptoma
Animal Diseases
Babesia bovis
Cattle
Macrophages
Transcriptome
Cepa Atenuada
Attenuated Strain
title_short Bovine macrophages transcriptome profiling reveals divergent responses to virulent and attenuated Babesia bovis strains
title_full Bovine macrophages transcriptome profiling reveals divergent responses to virulent and attenuated Babesia bovis strains
title_fullStr Bovine macrophages transcriptome profiling reveals divergent responses to virulent and attenuated Babesia bovis strains
title_full_unstemmed Bovine macrophages transcriptome profiling reveals divergent responses to virulent and attenuated Babesia bovis strains
title_sort Bovine macrophages transcriptome profiling reveals divergent responses to virulent and attenuated Babesia bovis strains
dc.creator.none.fl_str_mv Valenzano, Magali Nicole
Alvarez, Liliana
Valentini, Beatriz Susana
Wilkowsky, Silvina Elizabeth
author Valenzano, Magali Nicole
author_facet Valenzano, Magali Nicole
Alvarez, Liliana
Valentini, Beatriz Susana
Wilkowsky, Silvina Elizabeth
author_role author
author2 Alvarez, Liliana
Valentini, Beatriz Susana
Wilkowsky, Silvina Elizabeth
author2_role author
author
author
dc.subject.none.fl_str_mv Enfermedades de los Animales
Ganado Bovino
Macrófogos
Transcriptoma
Animal Diseases
Babesia bovis
Cattle
Macrophages
Transcriptome
Cepa Atenuada
Attenuated Strain
topic Enfermedades de los Animales
Ganado Bovino
Macrófogos
Transcriptoma
Animal Diseases
Babesia bovis
Cattle
Macrophages
Transcriptome
Cepa Atenuada
Attenuated Strain
dc.description.none.fl_txt_mv Babesia bovis is a tick-borne parasite of major economic impact in the livestock industry. Control strategies rely mainly on the use of acaricides and live attenuated vaccines. Comparative genomic analyses have shown no major differences between virulent and attenuated B. bovis strains, suggesting that studies on the host’s differential response may represent a key step toward clarifying the basis of disease severity and vaccine efficacy. In this study, we analyzed by RNA-seq the differential gene expression in bovine monocyte-derived macrophages after phagocytosis of erythrocytes infected with either the virulent B. bovis S2P strain or the attenuated R1A one. The results revealed a common transcriptional core response of several cellular processes largely centered on lymphocyte related functions, cytokine regulation, and adaptive immune signaling. In turn, the two strains elicited contrasting responses in bovine macrophages, where the virulent strain induced the enrichment of lymphocyte- and antiviral-related pathways, and the attenuated strain led to a stronger pro-inflammatory, chemotactic, and extracellular matrix remodeling signatures. Taken together, these data improved our understanding of the early transcriptional events that develop in macrophages in response to the phagocytosis of red blood cells containing contrasting B. bovis strains. This large dataset could be evaluated in further studies to better characterize the pathogenic mechanisms of bovine babesiosis and to identify targets for host-directed therapeutic strategies.
Instituto de Biotecnología
Fil: Valenzano, Magali Nicole. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina
Fil: Valenzano, Magali Nicole. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Alvarez, Liliana. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina
Fil: Alvarez, Liliana. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Valentini, Beatriz Susana. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rafaela; Argentina
Fil: Wilkowsky, Silvina Elizabeth. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina
Fil: Wilkowsky, Silvina Elizabeth. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
description Babesia bovis is a tick-borne parasite of major economic impact in the livestock industry. Control strategies rely mainly on the use of acaricides and live attenuated vaccines. Comparative genomic analyses have shown no major differences between virulent and attenuated B. bovis strains, suggesting that studies on the host’s differential response may represent a key step toward clarifying the basis of disease severity and vaccine efficacy. In this study, we analyzed by RNA-seq the differential gene expression in bovine monocyte-derived macrophages after phagocytosis of erythrocytes infected with either the virulent B. bovis S2P strain or the attenuated R1A one. The results revealed a common transcriptional core response of several cellular processes largely centered on lymphocyte related functions, cytokine regulation, and adaptive immune signaling. In turn, the two strains elicited contrasting responses in bovine macrophages, where the virulent strain induced the enrichment of lymphocyte- and antiviral-related pathways, and the attenuated strain led to a stronger pro-inflammatory, chemotactic, and extracellular matrix remodeling signatures. Taken together, these data improved our understanding of the early transcriptional events that develop in macrophages in response to the phagocytosis of red blood cells containing contrasting B. bovis strains. This large dataset could be evaluated in further studies to better characterize the pathogenic mechanisms of bovine babesiosis and to identify targets for host-directed therapeutic strategies.
publishDate 2026
dc.date.none.fl_str_mv 2026-02-18T13:49:17Z
2026-02-18T13:49:17Z
2026-01
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/20.500.12123/25221
https://www.sciencedirect.com/science/article/abs/pii/S0304401725002456
0304-4017
1873-2550
https://doi.org/10.1016/j.vetpar.2025.110634
url http://hdl.handle.net/20.500.12123/25221
https://www.sciencedirect.com/science/article/abs/pii/S0304401725002456
https://doi.org/10.1016/j.vetpar.2025.110634
identifier_str_mv 0304-4017
1873-2550
dc.language.none.fl_str_mv eng
language eng
dc.rights.none.fl_str_mv info:eu-repo/semantics/restrictedAccess
http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
eu_rights_str_mv restrictedAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv Veterinary Parasitology 341 : 110634. (January 2026)
reponame:INTA Digital (INTA)
instname:Instituto Nacional de Tecnología Agropecuaria
reponame_str INTA Digital (INTA)
collection INTA Digital (INTA)
instname_str Instituto Nacional de Tecnología Agropecuaria
repository.name.fl_str_mv INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuaria
repository.mail.fl_str_mv tripaldi.nicolas@inta.gob.ar
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score 13.176822

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