Genome-wide analysis of peptidase content and expression in a virulent and attenuated Babesia bovis strain pair

Autores
Mesplet, Maria; Palmer, Guy H.; Pedroni, Monica J.; Echaide, Ignacio Eduardo; Florin Christensen, Monica; Schnittger, Leonhard; Lau, Audrey O. T.
Año de publicación
2011
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Identifying virulence determinants in Apicomplexan parasites remains a major gap in knowledge for members within this phylum. We hypothesized that peptidases would segregate with virulence between a virulent parent Babesia bovis strain and an attenuated daughter strain derived by rapid in vivo passage. Using the complete genome sequence of the virulent T2Bo strain, 66 peptidases were identified and active sites confirmed. The presence, sequence identity and expression levels were tested for each of the 66 peptidases in the virulent parent and attenuated daughter T2Bo strains using whole genome, targeted sequencing approaches and microarrays analyses. Quantitative PCR revealed that there was no significant difference in peptidase expression between the virulent and attenuated strains. We conclude that while peptidases may well play a required role in B. bovis pathogenesis, neither loss of peptidase gene content nor reduced gene expression underlies the loss of virulence associated with in vivo passage and attenuation.
Fil: Mesplet, Maria. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Patobiología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Palmer, Guy H.. Washington State University; Estados Unidos
Fil: Pedroni, Monica J.. Washington State University; Estados Unidos
Fil: Echaide, Ignacio Eduardo. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Santa Fe. Estación Experimental Agropecuaria Rafaela; Argentina
Fil: Florin Christensen, Monica. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Patobiología; Argentina
Fil: Schnittger, Leonhard. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Patobiología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Lau, Audrey O. T.. Washington State University; Estados Unidos
Materia
APICOMPLEXANS
BABESIA BOVIS
PEPTIDASES
TRANSCRIPTOME
VIRULENCE
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/192941
Acceder
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network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Genome-wide analysis of peptidase content and expression in a virulent and attenuated Babesia bovis strain pairMesplet, MariaPalmer, Guy H.Pedroni, Monica J.Echaide, Ignacio EduardoFlorin Christensen, MonicaSchnittger, LeonhardLau, Audrey O. T.APICOMPLEXANSBABESIA BOVISPEPTIDASESTRANSCRIPTOMEVIRULENCEhttps://purl.org/becyt/ford/4.3https://purl.org/becyt/ford/4Identifying virulence determinants in Apicomplexan parasites remains a major gap in knowledge for members within this phylum. We hypothesized that peptidases would segregate with virulence between a virulent parent Babesia bovis strain and an attenuated daughter strain derived by rapid in vivo passage. Using the complete genome sequence of the virulent T2Bo strain, 66 peptidases were identified and active sites confirmed. The presence, sequence identity and expression levels were tested for each of the 66 peptidases in the virulent parent and attenuated daughter T2Bo strains using whole genome, targeted sequencing approaches and microarrays analyses. Quantitative PCR revealed that there was no significant difference in peptidase expression between the virulent and attenuated strains. We conclude that while peptidases may well play a required role in B. bovis pathogenesis, neither loss of peptidase gene content nor reduced gene expression underlies the loss of virulence associated with in vivo passage and attenuation.Fil: Mesplet, Maria. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Patobiología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Palmer, Guy H.. Washington State University; Estados UnidosFil: Pedroni, Monica J.. Washington State University; Estados UnidosFil: Echaide, Ignacio Eduardo. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Santa Fe. Estación Experimental Agropecuaria Rafaela; ArgentinaFil: Florin Christensen, Monica. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Patobiología; ArgentinaFil: Schnittger, Leonhard. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Patobiología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Lau, Audrey O. T.. Washington State University; Estados UnidosElsevier Science2011-06info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/192941Mesplet, Maria; Palmer, Guy H.; Pedroni, Monica J.; Echaide, Ignacio Eduardo; Florin Christensen, Monica; et al.; Genome-wide analysis of peptidase content and expression in a virulent and attenuated Babesia bovis strain pair; Elsevier Science; Molecular and Biochemical Parasitology; 179; 2; 6-2011; 111-1130166-6851CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1016/j.molbiopara.2011.06.005info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T09:38:19Zoai:ri.conicet.gov.ar:11336/192941instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 09:38:20.021CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Genome-wide analysis of peptidase content and expression in a virulent and attenuated Babesia bovis strain pair
title Genome-wide analysis of peptidase content and expression in a virulent and attenuated Babesia bovis strain pair
spellingShingle Genome-wide analysis of peptidase content and expression in a virulent and attenuated Babesia bovis strain pair
Mesplet, Maria
APICOMPLEXANS
BABESIA BOVIS
PEPTIDASES
TRANSCRIPTOME
VIRULENCE
title_short Genome-wide analysis of peptidase content and expression in a virulent and attenuated Babesia bovis strain pair
title_full Genome-wide analysis of peptidase content and expression in a virulent and attenuated Babesia bovis strain pair
title_fullStr Genome-wide analysis of peptidase content and expression in a virulent and attenuated Babesia bovis strain pair
title_full_unstemmed Genome-wide analysis of peptidase content and expression in a virulent and attenuated Babesia bovis strain pair
title_sort Genome-wide analysis of peptidase content and expression in a virulent and attenuated Babesia bovis strain pair
dc.creator.none.fl_str_mv Mesplet, Maria
Palmer, Guy H.
Pedroni, Monica J.
Echaide, Ignacio Eduardo
Florin Christensen, Monica
Schnittger, Leonhard
Lau, Audrey O. T.
author Mesplet, Maria
author_facet Mesplet, Maria
Palmer, Guy H.
Pedroni, Monica J.
Echaide, Ignacio Eduardo
Florin Christensen, Monica
Schnittger, Leonhard
Lau, Audrey O. T.
author_role author
author2 Palmer, Guy H.
Pedroni, Monica J.
Echaide, Ignacio Eduardo
Florin Christensen, Monica
Schnittger, Leonhard
Lau, Audrey O. T.
author2_role author
author
author
author
author
author
dc.subject.none.fl_str_mv APICOMPLEXANS
BABESIA BOVIS
PEPTIDASES
TRANSCRIPTOME
VIRULENCE
topic APICOMPLEXANS
BABESIA BOVIS
PEPTIDASES
TRANSCRIPTOME
VIRULENCE
purl_subject.fl_str_mv https://purl.org/becyt/ford/4.3
https://purl.org/becyt/ford/4
dc.description.none.fl_txt_mv Identifying virulence determinants in Apicomplexan parasites remains a major gap in knowledge for members within this phylum. We hypothesized that peptidases would segregate with virulence between a virulent parent Babesia bovis strain and an attenuated daughter strain derived by rapid in vivo passage. Using the complete genome sequence of the virulent T2Bo strain, 66 peptidases were identified and active sites confirmed. The presence, sequence identity and expression levels were tested for each of the 66 peptidases in the virulent parent and attenuated daughter T2Bo strains using whole genome, targeted sequencing approaches and microarrays analyses. Quantitative PCR revealed that there was no significant difference in peptidase expression between the virulent and attenuated strains. We conclude that while peptidases may well play a required role in B. bovis pathogenesis, neither loss of peptidase gene content nor reduced gene expression underlies the loss of virulence associated with in vivo passage and attenuation.
Fil: Mesplet, Maria. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Patobiología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Palmer, Guy H.. Washington State University; Estados Unidos
Fil: Pedroni, Monica J.. Washington State University; Estados Unidos
Fil: Echaide, Ignacio Eduardo. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Santa Fe. Estación Experimental Agropecuaria Rafaela; Argentina
Fil: Florin Christensen, Monica. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Patobiología; Argentina
Fil: Schnittger, Leonhard. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Patobiología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Lau, Audrey O. T.. Washington State University; Estados Unidos
description Identifying virulence determinants in Apicomplexan parasites remains a major gap in knowledge for members within this phylum. We hypothesized that peptidases would segregate with virulence between a virulent parent Babesia bovis strain and an attenuated daughter strain derived by rapid in vivo passage. Using the complete genome sequence of the virulent T2Bo strain, 66 peptidases were identified and active sites confirmed. The presence, sequence identity and expression levels were tested for each of the 66 peptidases in the virulent parent and attenuated daughter T2Bo strains using whole genome, targeted sequencing approaches and microarrays analyses. Quantitative PCR revealed that there was no significant difference in peptidase expression between the virulent and attenuated strains. We conclude that while peptidases may well play a required role in B. bovis pathogenesis, neither loss of peptidase gene content nor reduced gene expression underlies the loss of virulence associated with in vivo passage and attenuation.
publishDate 2011
dc.date.none.fl_str_mv 2011-06
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/192941
Mesplet, Maria; Palmer, Guy H.; Pedroni, Monica J.; Echaide, Ignacio Eduardo; Florin Christensen, Monica; et al.; Genome-wide analysis of peptidase content and expression in a virulent and attenuated Babesia bovis strain pair; Elsevier Science; Molecular and Biochemical Parasitology; 179; 2; 6-2011; 111-113
0166-6851
CONICET Digital
CONICET
url http://hdl.handle.net/11336/192941
identifier_str_mv Mesplet, Maria; Palmer, Guy H.; Pedroni, Monica J.; Echaide, Ignacio Eduardo; Florin Christensen, Monica; et al.; Genome-wide analysis of peptidase content and expression in a virulent and attenuated Babesia bovis strain pair; Elsevier Science; Molecular and Biochemical Parasitology; 179; 2; 6-2011; 111-113
0166-6851
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1016/j.molbiopara.2011.06.005
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier Science
publisher.none.fl_str_mv Elsevier Science
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1844613210894761984
score 13.070432

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