Alfalfa snakin-1 prevents fungal colonization and probably coevolved with rhizobia

Autores
Garcia, Araceli Nora; Ayub, Nicolás Daniel; Fox, Ana Romina; Gomez, Maria Cristina; Dieguez, Maria Jose; Pagano, Elba Maria; Berini, Carolina Andrea; Muschietti, Jorge Prometeo; Soto, Gabriela Cinthia
Año de publicación
2014
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Background: The production of antimicrobial peptides is a common defense strategy of living cells against a wide range of pathogens. Plant snakin peptides inhibit bacterial and fungal growth at extremely low concentrations. However, little is known of their molecular and ecological characteristics, including origin, evolutionary equivalence, specific functions and activity against beneficial microbes. The aim of this study was to identify and characterize snakin-1 from alfalfa (MsSN1). Results: Phylogenetic analysis showed complete congruence between snakin-1 and plant trees. The antimicrobial activity of MsSN1 against bacterial and fungal pathogens of alfalfa was demonstrated in vitro and in vivo. Transgenic alfalfa overexpressing MsSN1 showed increased antimicrobial activity against virulent fungal strains. However, MsSN1 did not affect nitrogen-fixing bacterial strains only when these had an alfalfa origin. Conclusions: The results reported here suggest that snakin peptides have important and ancestral roles in land plant innate immunity. Our data indicate a coevolutionary process, in which alfalfa exerts a selection pressure for resistance to MsSN1 on rhizobial bacteria. The increased antimicrobial activity against virulent fungal strains without altering the nitrogen-fixing symbiosis observed in MsSN1-overexpressing alfalfa transgenic plants opens the way to the production of effective legume transgenic cultivars for biotic stress resistance.
Instituto de Genética
Fil: Garcia, Araceli Nora. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética; Argentina
Fil: Ayub, Nicolás Daniel. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética; Argentina
Fil: Fox, Ana Romina. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética; Argentina.
Fil: Gomez, Maria Cristina. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética; Argentina
Fil: Dieguez, María José. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética; Argentina
Fil: Pagano, Elba Maria. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética; Argentina
Fil: Berini, Carolina Andrea. Instituto de Investigaciones Biomédicas en Retrovirus y SIDA; Argentina
Fil: Muschietti, Jorge Prometeo. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Biodiversidad y Biología Experimental; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular, “Dr. Hector Torres”; Argentina
Fil: Soto, Gabriela Cinthia. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética; Argentina
Fuente
BMC Plant Biology 14: 248 (Sep 2014)
Materia
Medicago sativa
Péptidos
Antimicrobianos
Resistencia a la Enfermedad
Peptides
Antimicrobials
Disease Resistance
Alfalfa
Nivel de accesibilidad
acceso abierto
Condiciones de uso
http://creativecommons.org/licenses/by-nc-sa/4.0/
Repositorio
INTA Digital (INTA)
Institución
Instituto Nacional de Tecnología Agropecuaria
OAI Identificador
oai:localhost:20.500.12123/3438
Acceder
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oai_identifier_str oai:localhost:20.500.12123/3438
network_acronym_str INTADig
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network_name_str INTA Digital (INTA)
spelling Alfalfa snakin-1 prevents fungal colonization and probably coevolved with rhizobiaGarcia, Araceli NoraAyub, Nicolás DanielFox, Ana RominaGomez, Maria CristinaDieguez, Maria JosePagano, Elba MariaBerini, Carolina AndreaMuschietti, Jorge PrometeoSoto, Gabriela CinthiaMedicago sativaPéptidosAntimicrobianosResistencia a la EnfermedadPeptidesAntimicrobialsDisease ResistanceAlfalfaBackground: The production of antimicrobial peptides is a common defense strategy of living cells against a wide range of pathogens. Plant snakin peptides inhibit bacterial and fungal growth at extremely low concentrations. However, little is known of their molecular and ecological characteristics, including origin, evolutionary equivalence, specific functions and activity against beneficial microbes. The aim of this study was to identify and characterize snakin-1 from alfalfa (MsSN1). Results: Phylogenetic analysis showed complete congruence between snakin-1 and plant trees. The antimicrobial activity of MsSN1 against bacterial and fungal pathogens of alfalfa was demonstrated in vitro and in vivo. Transgenic alfalfa overexpressing MsSN1 showed increased antimicrobial activity against virulent fungal strains. However, MsSN1 did not affect nitrogen-fixing bacterial strains only when these had an alfalfa origin. Conclusions: The results reported here suggest that snakin peptides have important and ancestral roles in land plant innate immunity. Our data indicate a coevolutionary process, in which alfalfa exerts a selection pressure for resistance to MsSN1 on rhizobial bacteria. The increased antimicrobial activity against virulent fungal strains without altering the nitrogen-fixing symbiosis observed in MsSN1-overexpressing alfalfa transgenic plants opens the way to the production of effective legume transgenic cultivars for biotic stress resistance.Instituto de GenéticaFil: Garcia, Araceli Nora. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética; ArgentinaFil: Ayub, Nicolás Daniel. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética; ArgentinaFil: Fox, Ana Romina. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética; Argentina.Fil: Gomez, Maria Cristina. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética; ArgentinaFil: Dieguez, María José. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética; ArgentinaFil: Pagano, Elba Maria. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética; ArgentinaFil: Berini, Carolina Andrea. Instituto de Investigaciones Biomédicas en Retrovirus y SIDA; ArgentinaFil: Muschietti, Jorge Prometeo. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Biodiversidad y Biología Experimental; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular, “Dr. Hector Torres”; ArgentinaFil: Soto, Gabriela Cinthia. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética; ArgentinaBMC Plant Biology2018-09-21T11:13:41Z2018-09-21T11:13:41Z2014-09-17info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttps://bmcplantbiol.biomedcentral.com/articles/10.1186/s12870-014-0248-9http://hdl.handle.net/20.500.12123/34381471-2229https://doi.org/10.1186/s12870-014-0248-9BMC Plant Biology 14: 248 (Sep 2014)reponame:INTA Digital (INTA)instname:Instituto Nacional de Tecnología Agropecuariaenginfo:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by-nc-sa/4.0/Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)2025-09-29T13:44:26Zoai:localhost:20.500.12123/3438instacron:INTAInstitucionalhttp://repositorio.inta.gob.ar/Organismo científico-tecnológicoNo correspondehttp://repositorio.inta.gob.ar/oai/requesttripaldi.nicolas@inta.gob.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:l2025-09-29 13:44:26.894INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuariafalse
dc.title.none.fl_str_mv Alfalfa snakin-1 prevents fungal colonization and probably coevolved with rhizobia
title Alfalfa snakin-1 prevents fungal colonization and probably coevolved with rhizobia
spellingShingle Alfalfa snakin-1 prevents fungal colonization and probably coevolved with rhizobia
Garcia, Araceli Nora
Medicago sativa
Péptidos
Antimicrobianos
Resistencia a la Enfermedad
Peptides
Antimicrobials
Disease Resistance
Alfalfa
title_short Alfalfa snakin-1 prevents fungal colonization and probably coevolved with rhizobia
title_full Alfalfa snakin-1 prevents fungal colonization and probably coevolved with rhizobia
title_fullStr Alfalfa snakin-1 prevents fungal colonization and probably coevolved with rhizobia
title_full_unstemmed Alfalfa snakin-1 prevents fungal colonization and probably coevolved with rhizobia
title_sort Alfalfa snakin-1 prevents fungal colonization and probably coevolved with rhizobia
dc.creator.none.fl_str_mv Garcia, Araceli Nora
Ayub, Nicolás Daniel
Fox, Ana Romina
Gomez, Maria Cristina
Dieguez, Maria Jose
Pagano, Elba Maria
Berini, Carolina Andrea
Muschietti, Jorge Prometeo
Soto, Gabriela Cinthia
author Garcia, Araceli Nora
author_facet Garcia, Araceli Nora
Ayub, Nicolás Daniel
Fox, Ana Romina
Gomez, Maria Cristina
Dieguez, Maria Jose
Pagano, Elba Maria
Berini, Carolina Andrea
Muschietti, Jorge Prometeo
Soto, Gabriela Cinthia
author_role author
author2 Ayub, Nicolás Daniel
Fox, Ana Romina
Gomez, Maria Cristina
Dieguez, Maria Jose
Pagano, Elba Maria
Berini, Carolina Andrea
Muschietti, Jorge Prometeo
Soto, Gabriela Cinthia
author2_role author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Medicago sativa
Péptidos
Antimicrobianos
Resistencia a la Enfermedad
Peptides
Antimicrobials
Disease Resistance
Alfalfa
topic Medicago sativa
Péptidos
Antimicrobianos
Resistencia a la Enfermedad
Peptides
Antimicrobials
Disease Resistance
Alfalfa
dc.description.none.fl_txt_mv Background: The production of antimicrobial peptides is a common defense strategy of living cells against a wide range of pathogens. Plant snakin peptides inhibit bacterial and fungal growth at extremely low concentrations. However, little is known of their molecular and ecological characteristics, including origin, evolutionary equivalence, specific functions and activity against beneficial microbes. The aim of this study was to identify and characterize snakin-1 from alfalfa (MsSN1). Results: Phylogenetic analysis showed complete congruence between snakin-1 and plant trees. The antimicrobial activity of MsSN1 against bacterial and fungal pathogens of alfalfa was demonstrated in vitro and in vivo. Transgenic alfalfa overexpressing MsSN1 showed increased antimicrobial activity against virulent fungal strains. However, MsSN1 did not affect nitrogen-fixing bacterial strains only when these had an alfalfa origin. Conclusions: The results reported here suggest that snakin peptides have important and ancestral roles in land plant innate immunity. Our data indicate a coevolutionary process, in which alfalfa exerts a selection pressure for resistance to MsSN1 on rhizobial bacteria. The increased antimicrobial activity against virulent fungal strains without altering the nitrogen-fixing symbiosis observed in MsSN1-overexpressing alfalfa transgenic plants opens the way to the production of effective legume transgenic cultivars for biotic stress resistance.
Instituto de Genética
Fil: Garcia, Araceli Nora. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética; Argentina
Fil: Ayub, Nicolás Daniel. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética; Argentina
Fil: Fox, Ana Romina. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética; Argentina.
Fil: Gomez, Maria Cristina. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética; Argentina
Fil: Dieguez, María José. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética; Argentina
Fil: Pagano, Elba Maria. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética; Argentina
Fil: Berini, Carolina Andrea. Instituto de Investigaciones Biomédicas en Retrovirus y SIDA; Argentina
Fil: Muschietti, Jorge Prometeo. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Biodiversidad y Biología Experimental; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular, “Dr. Hector Torres”; Argentina
Fil: Soto, Gabriela Cinthia. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética; Argentina
description Background: The production of antimicrobial peptides is a common defense strategy of living cells against a wide range of pathogens. Plant snakin peptides inhibit bacterial and fungal growth at extremely low concentrations. However, little is known of their molecular and ecological characteristics, including origin, evolutionary equivalence, specific functions and activity against beneficial microbes. The aim of this study was to identify and characterize snakin-1 from alfalfa (MsSN1). Results: Phylogenetic analysis showed complete congruence between snakin-1 and plant trees. The antimicrobial activity of MsSN1 against bacterial and fungal pathogens of alfalfa was demonstrated in vitro and in vivo. Transgenic alfalfa overexpressing MsSN1 showed increased antimicrobial activity against virulent fungal strains. However, MsSN1 did not affect nitrogen-fixing bacterial strains only when these had an alfalfa origin. Conclusions: The results reported here suggest that snakin peptides have important and ancestral roles in land plant innate immunity. Our data indicate a coevolutionary process, in which alfalfa exerts a selection pressure for resistance to MsSN1 on rhizobial bacteria. The increased antimicrobial activity against virulent fungal strains without altering the nitrogen-fixing symbiosis observed in MsSN1-overexpressing alfalfa transgenic plants opens the way to the production of effective legume transgenic cultivars for biotic stress resistance.
publishDate 2014
dc.date.none.fl_str_mv 2014-09-17
2018-09-21T11:13:41Z
2018-09-21T11:13:41Z
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv https://bmcplantbiol.biomedcentral.com/articles/10.1186/s12870-014-0248-9
http://hdl.handle.net/20.500.12123/3438
1471-2229
https://doi.org/10.1186/s12870-014-0248-9
url https://bmcplantbiol.biomedcentral.com/articles/10.1186/s12870-014-0248-9
http://hdl.handle.net/20.500.12123/3438
https://doi.org/10.1186/s12870-014-0248-9
identifier_str_mv 1471-2229
dc.language.none.fl_str_mv eng
language eng
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
eu_rights_str_mv openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv BMC Plant Biology
publisher.none.fl_str_mv BMC Plant Biology
dc.source.none.fl_str_mv BMC Plant Biology 14: 248 (Sep 2014)
reponame:INTA Digital (INTA)
instname:Instituto Nacional de Tecnología Agropecuaria
reponame_str INTA Digital (INTA)
collection INTA Digital (INTA)
instname_str Instituto Nacional de Tecnología Agropecuaria
repository.name.fl_str_mv INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuaria
repository.mail.fl_str_mv tripaldi.nicolas@inta.gob.ar
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score 12.559606

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