Pseudomonas fluorescens Pf-5 genome-wide mutant screen for resistance to the antimicrobial peptide alfalfa snakin-1

Autores
Ayub, Nicolás Daniel; Fox, Ana Romina; Garcia, Araceli Nora; Mozzicafreddo, Matteo; Cuccioloni, Massimiliano; Angeletti, Mauro; Pagano, Elba Maria; Soto, Gabriela Cynthia
Año de publicación
2015
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Snakin-1, a peptide produced by higher plants, has broad-spectrum antibiotic activity, inhibiting organisms ranging from Bacteria to Eukaryotes. However, the mode of action against target organisms is poorly understood. As a first step to elucidate the mechanism, we screened a mutation library of Pseudomonas fluorescens Pf-5 in LB and agar medium supplemented with alfalfa snakin-1 (MsSN1). We identified three biofilm formation-related Pseudomonas mutants that showed increased resistance to MsSN1. Genetic, physiological and bioinformatics analysis validated the results of the mutant screens, indicating that bacterial adhesion protein lapA is probably the target of MsSN1. Collectively, these findings suggest that snakin-1 acts on microbial adhesion properties.
Instituto de Genética
Fil: Ayub, Nicolás Daniel. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Fox, Ana Romina. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Garcia, Araceli Nora. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética; Argentina
Fil: Mozzicafreddo, Matteo. University of Camerino. School of Biosciences and Biotechnology; Italia
Fil: Cuccioloni, Massimiliano. University of Camerino. School of Biosciences and Biotechnology; Italia
Fil: Angeletti, Mauro. University of Camerino. School of Biosciences and Biotechnology; Italia
Fil: Pagano, Elba Maria. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética; Argentina
Fil: Soto, Gabriela Cinthia. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fuente
FEMS Microbiology Letters 362 (2) : 1-6 (Enero 2015)
Materia
Immunity
Antimicrobial Agents
Peptides
Defence Mechanisms
Inmunidad
Agente Antimicrobiano
Péptidos
Pseudomonas
Mecanismos de Defensa
Medicago sativa
Alfalfa
Nivel de accesibilidad
acceso abierto
Condiciones de uso
http://creativecommons.org/licenses/by-nc-sa/4.0/
Repositorio
INTA Digital (INTA)
Institución
Instituto Nacional de Tecnología Agropecuaria
OAI Identificador
oai:localhost:20.500.12123/8712
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oai_identifier_str oai:localhost:20.500.12123/8712
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spelling Pseudomonas fluorescens Pf-5 genome-wide mutant screen for resistance to the antimicrobial peptide alfalfa snakin-1Ayub, Nicolás DanielFox, Ana RominaGarcia, Araceli NoraMozzicafreddo, MatteoCuccioloni, MassimilianoAngeletti, MauroPagano, Elba MariaSoto, Gabriela CynthiaImmunityAntimicrobial AgentsPeptidesDefence MechanismsInmunidadAgente AntimicrobianoPéptidosPseudomonasMecanismos de DefensaMedicago sativaAlfalfaSnakin-1, a peptide produced by higher plants, has broad-spectrum antibiotic activity, inhibiting organisms ranging from Bacteria to Eukaryotes. However, the mode of action against target organisms is poorly understood. As a first step to elucidate the mechanism, we screened a mutation library of Pseudomonas fluorescens Pf-5 in LB and agar medium supplemented with alfalfa snakin-1 (MsSN1). We identified three biofilm formation-related Pseudomonas mutants that showed increased resistance to MsSN1. Genetic, physiological and bioinformatics analysis validated the results of the mutant screens, indicating that bacterial adhesion protein lapA is probably the target of MsSN1. Collectively, these findings suggest that snakin-1 acts on microbial adhesion properties.Instituto de GenéticaFil: Ayub, Nicolás Daniel. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Fox, Ana Romina. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Garcia, Araceli Nora. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética; ArgentinaFil: Mozzicafreddo, Matteo. University of Camerino. School of Biosciences and Biotechnology; ItaliaFil: Cuccioloni, Massimiliano. University of Camerino. School of Biosciences and Biotechnology; ItaliaFil: Angeletti, Mauro. University of Camerino. School of Biosciences and Biotechnology; ItaliaFil: Pagano, Elba Maria. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética; ArgentinaFil: Soto, Gabriela Cinthia. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaOxford University Press2021-02-22T12:43:22Z2021-02-22T12:43:22Z2015-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://hdl.handle.net/20.500.12123/8712https://academic.oup.com/femsle/article/362/2/1/24673741574-6968https://doi.org/10.1093/femsle/fnu006FEMS Microbiology Letters 362 (2) : 1-6 (Enero 2015)reponame:INTA Digital (INTA)instname:Instituto Nacional de Tecnología Agropecuariaenginfo:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by-nc-sa/4.0/Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)2025-09-29T13:45:08Zoai:localhost:20.500.12123/8712instacron:INTAInstitucionalhttp://repositorio.inta.gob.ar/Organismo científico-tecnológicoNo correspondehttp://repositorio.inta.gob.ar/oai/requesttripaldi.nicolas@inta.gob.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:l2025-09-29 13:45:08.5INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuariafalse
dc.title.none.fl_str_mv Pseudomonas fluorescens Pf-5 genome-wide mutant screen for resistance to the antimicrobial peptide alfalfa snakin-1
title Pseudomonas fluorescens Pf-5 genome-wide mutant screen for resistance to the antimicrobial peptide alfalfa snakin-1
spellingShingle Pseudomonas fluorescens Pf-5 genome-wide mutant screen for resistance to the antimicrobial peptide alfalfa snakin-1
Ayub, Nicolás Daniel
Immunity
Antimicrobial Agents
Peptides
Defence Mechanisms
Inmunidad
Agente Antimicrobiano
Péptidos
Pseudomonas
Mecanismos de Defensa
Medicago sativa
Alfalfa
title_short Pseudomonas fluorescens Pf-5 genome-wide mutant screen for resistance to the antimicrobial peptide alfalfa snakin-1
title_full Pseudomonas fluorescens Pf-5 genome-wide mutant screen for resistance to the antimicrobial peptide alfalfa snakin-1
title_fullStr Pseudomonas fluorescens Pf-5 genome-wide mutant screen for resistance to the antimicrobial peptide alfalfa snakin-1
title_full_unstemmed Pseudomonas fluorescens Pf-5 genome-wide mutant screen for resistance to the antimicrobial peptide alfalfa snakin-1
title_sort Pseudomonas fluorescens Pf-5 genome-wide mutant screen for resistance to the antimicrobial peptide alfalfa snakin-1
dc.creator.none.fl_str_mv Ayub, Nicolás Daniel
Fox, Ana Romina
Garcia, Araceli Nora
Mozzicafreddo, Matteo
Cuccioloni, Massimiliano
Angeletti, Mauro
Pagano, Elba Maria
Soto, Gabriela Cynthia
author Ayub, Nicolás Daniel
author_facet Ayub, Nicolás Daniel
Fox, Ana Romina
Garcia, Araceli Nora
Mozzicafreddo, Matteo
Cuccioloni, Massimiliano
Angeletti, Mauro
Pagano, Elba Maria
Soto, Gabriela Cynthia
author_role author
author2 Fox, Ana Romina
Garcia, Araceli Nora
Mozzicafreddo, Matteo
Cuccioloni, Massimiliano
Angeletti, Mauro
Pagano, Elba Maria
Soto, Gabriela Cynthia
author2_role author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Immunity
Antimicrobial Agents
Peptides
Defence Mechanisms
Inmunidad
Agente Antimicrobiano
Péptidos
Pseudomonas
Mecanismos de Defensa
Medicago sativa
Alfalfa
topic Immunity
Antimicrobial Agents
Peptides
Defence Mechanisms
Inmunidad
Agente Antimicrobiano
Péptidos
Pseudomonas
Mecanismos de Defensa
Medicago sativa
Alfalfa
dc.description.none.fl_txt_mv Snakin-1, a peptide produced by higher plants, has broad-spectrum antibiotic activity, inhibiting organisms ranging from Bacteria to Eukaryotes. However, the mode of action against target organisms is poorly understood. As a first step to elucidate the mechanism, we screened a mutation library of Pseudomonas fluorescens Pf-5 in LB and agar medium supplemented with alfalfa snakin-1 (MsSN1). We identified three biofilm formation-related Pseudomonas mutants that showed increased resistance to MsSN1. Genetic, physiological and bioinformatics analysis validated the results of the mutant screens, indicating that bacterial adhesion protein lapA is probably the target of MsSN1. Collectively, these findings suggest that snakin-1 acts on microbial adhesion properties.
Instituto de Genética
Fil: Ayub, Nicolás Daniel. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Fox, Ana Romina. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Garcia, Araceli Nora. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética; Argentina
Fil: Mozzicafreddo, Matteo. University of Camerino. School of Biosciences and Biotechnology; Italia
Fil: Cuccioloni, Massimiliano. University of Camerino. School of Biosciences and Biotechnology; Italia
Fil: Angeletti, Mauro. University of Camerino. School of Biosciences and Biotechnology; Italia
Fil: Pagano, Elba Maria. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética; Argentina
Fil: Soto, Gabriela Cinthia. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
description Snakin-1, a peptide produced by higher plants, has broad-spectrum antibiotic activity, inhibiting organisms ranging from Bacteria to Eukaryotes. However, the mode of action against target organisms is poorly understood. As a first step to elucidate the mechanism, we screened a mutation library of Pseudomonas fluorescens Pf-5 in LB and agar medium supplemented with alfalfa snakin-1 (MsSN1). We identified three biofilm formation-related Pseudomonas mutants that showed increased resistance to MsSN1. Genetic, physiological and bioinformatics analysis validated the results of the mutant screens, indicating that bacterial adhesion protein lapA is probably the target of MsSN1. Collectively, these findings suggest that snakin-1 acts on microbial adhesion properties.
publishDate 2015
dc.date.none.fl_str_mv 2015-01
2021-02-22T12:43:22Z
2021-02-22T12:43:22Z
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/20.500.12123/8712
https://academic.oup.com/femsle/article/362/2/1/2467374
1574-6968
https://doi.org/10.1093/femsle/fnu006
url http://hdl.handle.net/20.500.12123/8712
https://academic.oup.com/femsle/article/362/2/1/2467374
https://doi.org/10.1093/femsle/fnu006
identifier_str_mv 1574-6968
dc.language.none.fl_str_mv eng
language eng
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
eu_rights_str_mv openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Oxford University Press
publisher.none.fl_str_mv Oxford University Press
dc.source.none.fl_str_mv FEMS Microbiology Letters 362 (2) : 1-6 (Enero 2015)
reponame:INTA Digital (INTA)
instname:Instituto Nacional de Tecnología Agropecuaria
reponame_str INTA Digital (INTA)
collection INTA Digital (INTA)
instname_str Instituto Nacional de Tecnología Agropecuaria
repository.name.fl_str_mv INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuaria
repository.mail.fl_str_mv tripaldi.nicolas@inta.gob.ar
_version_ 1844619150872281088
score 12.559606

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