Exploring the Genetic Networks of HLB Tolerance in Citrus: Insights Across Species and Tissues

Autores
Machado, Rodrigo; Moschen, Sebastian Nicolas; Conti, Gabriela; Gonzalez, Sergio Alberto; Rivarola, Maximo Lisandro; Gomez, Claudio Andres; Hopp, Horacio Esteban; Fernandez, Paula Del Carmen
Año de publicación
2025
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Huanglongbing (HLB), caused mainly by Candidatus Liberibacter asiaticus (CLas), is a devastating disease threatening citrus production worldwide, leading to leaf mottling, fruit deformation, and significant yield losses. This study generated a comprehensive co-expression network analysis using RNA-seq data from 17 public datasets. Weighted gene co-expression network analysis (WGCNA) was applied to identify gene modules associated with citrus species, tissue types, and days post-infection (DPIs). These modules revealed significant enrichment in biological pathways related to stress responses, metabolic reprograming, ribosomal protein synthesis, chloroplast and plastid function, cellular architecture, and intracellular transport. The results offer a molecular framework for understanding HLB pathogenesis and host response. By elucidating module-specific functions and their correlation with species- and tissue-specific responses, this study provides a robust foundation for identifying key genetic targets. These insights facilitate breeding programs focused on developing HLB-tolerant citrus cultivars, contributing to the long-term sustainability and resilience of global citrus production.
EEA Concordia
Fil: Machado, Rodrigo. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Concordia; Argentina.
Fil: Moschen, Sebastian Nicolas. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Famaillá; Argentina
Fil: Moschen, Sebastian Nicolas. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Conti, Gabriela. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina
Fil: Conti, Gabriela. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Gonzalez, Sergio Alberto. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina
Fil: Rivarola, Maximo Lisandro. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina
Fil: Rivarola, Maximo Lisandro. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Gomez, Claudio Andres. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Concordia; Argentina
Fil: Hopp, Horacio Esteban. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina
Fil: Fernandez, Paula Del Carmen. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina
Fil: Fernandez, Paula Del Carmen. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fuente
Plants 14 (12) : 1792. (June 2025)
Materia
Citrus
Plant Diseases
Huanglongbing
Genetics
Disease Tolerance
Enfermedades de las Plantas
Huanglongbing de los Cítricos
Genética
Tolerancia a Enfermedades
HLB
Candidatus Liberibacter
Nivel de accesibilidad
acceso abierto
Condiciones de uso
http://creativecommons.org/licenses/by-nc-sa/4.0/
Repositorio
INTA Digital (INTA)
Institución
Instituto Nacional de Tecnología Agropecuaria
OAI Identificador
oai:localhost:20.500.12123/22675
Acceder
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oai_identifier_str oai:localhost:20.500.12123/22675
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spelling Exploring the Genetic Networks of HLB Tolerance in Citrus: Insights Across Species and TissuesMachado, RodrigoMoschen, Sebastian NicolasConti, GabrielaGonzalez, Sergio AlbertoRivarola, Maximo LisandroGomez, Claudio AndresHopp, Horacio EstebanFernandez, Paula Del CarmenCitrusPlant DiseasesHuanglongbingGeneticsDisease ToleranceEnfermedades de las PlantasHuanglongbing de los CítricosGenéticaTolerancia a EnfermedadesHLBCandidatus LiberibacterHuanglongbing (HLB), caused mainly by Candidatus Liberibacter asiaticus (CLas), is a devastating disease threatening citrus production worldwide, leading to leaf mottling, fruit deformation, and significant yield losses. This study generated a comprehensive co-expression network analysis using RNA-seq data from 17 public datasets. Weighted gene co-expression network analysis (WGCNA) was applied to identify gene modules associated with citrus species, tissue types, and days post-infection (DPIs). These modules revealed significant enrichment in biological pathways related to stress responses, metabolic reprograming, ribosomal protein synthesis, chloroplast and plastid function, cellular architecture, and intracellular transport. The results offer a molecular framework for understanding HLB pathogenesis and host response. By elucidating module-specific functions and their correlation with species- and tissue-specific responses, this study provides a robust foundation for identifying key genetic targets. These insights facilitate breeding programs focused on developing HLB-tolerant citrus cultivars, contributing to the long-term sustainability and resilience of global citrus production.EEA ConcordiaFil: Machado, Rodrigo. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Concordia; Argentina.Fil: Moschen, Sebastian Nicolas. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Famaillá; ArgentinaFil: Moschen, Sebastian Nicolas. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Conti, Gabriela. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; ArgentinaFil: Conti, Gabriela. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Gonzalez, Sergio Alberto. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; ArgentinaFil: Rivarola, Maximo Lisandro. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; ArgentinaFil: Rivarola, Maximo Lisandro. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Gomez, Claudio Andres. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Concordia; ArgentinaFil: Hopp, Horacio Esteban. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; ArgentinaFil: Fernandez, Paula Del Carmen. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; ArgentinaFil: Fernandez, Paula Del Carmen. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaMDPI2025-06-13T11:22:15Z2025-06-13T11:22:15Z2025-06info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://hdl.handle.net/20.500.12123/22675https://www.mdpi.com/2223-7747/14/12/17922223-7747https://doi.org/10.3390/plants14121792Plants 14 (12) : 1792. (June 2025)reponame:INTA Digital (INTA)instname:Instituto Nacional de Tecnología Agropecuariaenginfo:eu-repograntAgreement/INTA/2019-PD-E4-I101-001, Prospección, Prevención y Control de Lobesia botrana, Drosophila suzukii, HLB y Carpocapsainfo:eu-repograntAgreement/INTA/2023-PD-L01-I106, Plagas y enfermedades emergentes, cuarentenarias y limitantes en cultivos frutícolas: estrategias para mejorar su prevención, detección precoz y controlinfo:eu-repograntAgreement/INTA/2023-PE-L01-I051, Fortalecimiento y modernización de la cadena citrícola nacional con enfoque en la sostenibilidad en un marco de cambio climáticoinfo:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by-nc-sa/4.0/Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)2025-09-29T13:47:21Zoai:localhost:20.500.12123/22675instacron:INTAInstitucionalhttp://repositorio.inta.gob.ar/Organismo científico-tecnológicoNo correspondehttp://repositorio.inta.gob.ar/oai/requesttripaldi.nicolas@inta.gob.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:l2025-09-29 13:47:21.66INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuariafalse
dc.title.none.fl_str_mv Exploring the Genetic Networks of HLB Tolerance in Citrus: Insights Across Species and Tissues
title Exploring the Genetic Networks of HLB Tolerance in Citrus: Insights Across Species and Tissues
spellingShingle Exploring the Genetic Networks of HLB Tolerance in Citrus: Insights Across Species and Tissues
Machado, Rodrigo
Citrus
Plant Diseases
Huanglongbing
Genetics
Disease Tolerance
Enfermedades de las Plantas
Huanglongbing de los Cítricos
Genética
Tolerancia a Enfermedades
HLB
Candidatus Liberibacter
title_short Exploring the Genetic Networks of HLB Tolerance in Citrus: Insights Across Species and Tissues
title_full Exploring the Genetic Networks of HLB Tolerance in Citrus: Insights Across Species and Tissues
title_fullStr Exploring the Genetic Networks of HLB Tolerance in Citrus: Insights Across Species and Tissues
title_full_unstemmed Exploring the Genetic Networks of HLB Tolerance in Citrus: Insights Across Species and Tissues
title_sort Exploring the Genetic Networks of HLB Tolerance in Citrus: Insights Across Species and Tissues
dc.creator.none.fl_str_mv Machado, Rodrigo
Moschen, Sebastian Nicolas
Conti, Gabriela
Gonzalez, Sergio Alberto
Rivarola, Maximo Lisandro
Gomez, Claudio Andres
Hopp, Horacio Esteban
Fernandez, Paula Del Carmen
author Machado, Rodrigo
author_facet Machado, Rodrigo
Moschen, Sebastian Nicolas
Conti, Gabriela
Gonzalez, Sergio Alberto
Rivarola, Maximo Lisandro
Gomez, Claudio Andres
Hopp, Horacio Esteban
Fernandez, Paula Del Carmen
author_role author
author2 Moschen, Sebastian Nicolas
Conti, Gabriela
Gonzalez, Sergio Alberto
Rivarola, Maximo Lisandro
Gomez, Claudio Andres
Hopp, Horacio Esteban
Fernandez, Paula Del Carmen
author2_role author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Citrus
Plant Diseases
Huanglongbing
Genetics
Disease Tolerance
Enfermedades de las Plantas
Huanglongbing de los Cítricos
Genética
Tolerancia a Enfermedades
HLB
Candidatus Liberibacter
topic Citrus
Plant Diseases
Huanglongbing
Genetics
Disease Tolerance
Enfermedades de las Plantas
Huanglongbing de los Cítricos
Genética
Tolerancia a Enfermedades
HLB
Candidatus Liberibacter
dc.description.none.fl_txt_mv Huanglongbing (HLB), caused mainly by Candidatus Liberibacter asiaticus (CLas), is a devastating disease threatening citrus production worldwide, leading to leaf mottling, fruit deformation, and significant yield losses. This study generated a comprehensive co-expression network analysis using RNA-seq data from 17 public datasets. Weighted gene co-expression network analysis (WGCNA) was applied to identify gene modules associated with citrus species, tissue types, and days post-infection (DPIs). These modules revealed significant enrichment in biological pathways related to stress responses, metabolic reprograming, ribosomal protein synthesis, chloroplast and plastid function, cellular architecture, and intracellular transport. The results offer a molecular framework for understanding HLB pathogenesis and host response. By elucidating module-specific functions and their correlation with species- and tissue-specific responses, this study provides a robust foundation for identifying key genetic targets. These insights facilitate breeding programs focused on developing HLB-tolerant citrus cultivars, contributing to the long-term sustainability and resilience of global citrus production.
EEA Concordia
Fil: Machado, Rodrigo. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Concordia; Argentina.
Fil: Moschen, Sebastian Nicolas. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Famaillá; Argentina
Fil: Moschen, Sebastian Nicolas. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Conti, Gabriela. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina
Fil: Conti, Gabriela. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Gonzalez, Sergio Alberto. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina
Fil: Rivarola, Maximo Lisandro. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina
Fil: Rivarola, Maximo Lisandro. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Gomez, Claudio Andres. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Concordia; Argentina
Fil: Hopp, Horacio Esteban. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina
Fil: Fernandez, Paula Del Carmen. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina
Fil: Fernandez, Paula Del Carmen. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
description Huanglongbing (HLB), caused mainly by Candidatus Liberibacter asiaticus (CLas), is a devastating disease threatening citrus production worldwide, leading to leaf mottling, fruit deformation, and significant yield losses. This study generated a comprehensive co-expression network analysis using RNA-seq data from 17 public datasets. Weighted gene co-expression network analysis (WGCNA) was applied to identify gene modules associated with citrus species, tissue types, and days post-infection (DPIs). These modules revealed significant enrichment in biological pathways related to stress responses, metabolic reprograming, ribosomal protein synthesis, chloroplast and plastid function, cellular architecture, and intracellular transport. The results offer a molecular framework for understanding HLB pathogenesis and host response. By elucidating module-specific functions and their correlation with species- and tissue-specific responses, this study provides a robust foundation for identifying key genetic targets. These insights facilitate breeding programs focused on developing HLB-tolerant citrus cultivars, contributing to the long-term sustainability and resilience of global citrus production.
publishDate 2025
dc.date.none.fl_str_mv 2025-06-13T11:22:15Z
2025-06-13T11:22:15Z
2025-06
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/20.500.12123/22675
https://www.mdpi.com/2223-7747/14/12/1792
2223-7747
https://doi.org/10.3390/plants14121792
url http://hdl.handle.net/20.500.12123/22675
https://www.mdpi.com/2223-7747/14/12/1792
https://doi.org/10.3390/plants14121792
identifier_str_mv 2223-7747
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repograntAgreement/INTA/2019-PD-E4-I101-001, Prospección, Prevención y Control de Lobesia botrana, Drosophila suzukii, HLB y Carpocapsa
info:eu-repograntAgreement/INTA/2023-PD-L01-I106, Plagas y enfermedades emergentes, cuarentenarias y limitantes en cultivos frutícolas: estrategias para mejorar su prevención, detección precoz y control
info:eu-repograntAgreement/INTA/2023-PE-L01-I051, Fortalecimiento y modernización de la cadena citrícola nacional con enfoque en la sostenibilidad en un marco de cambio climático
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
eu_rights_str_mv openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv MDPI
publisher.none.fl_str_mv MDPI
dc.source.none.fl_str_mv Plants 14 (12) : 1792. (June 2025)
reponame:INTA Digital (INTA)
instname:Instituto Nacional de Tecnología Agropecuaria
reponame_str INTA Digital (INTA)
collection INTA Digital (INTA)
instname_str Instituto Nacional de Tecnología Agropecuaria
repository.name.fl_str_mv INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuaria
repository.mail.fl_str_mv tripaldi.nicolas@inta.gob.ar
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