Detection and molecular characterization of a phytoplasma affecting Prunus persica L. in Jujuy, Argentina

Autores
Fernandez, Franco Daniel; Guzman, Fabiana; Curzel, Viviana; Bejarano, Noemí; Conci, Luis Rogelio
Año de publicación
2013
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Peach (Prunus persica L.) plants with symptoms of yellowing, reddening, curling and leaf necrosis, premature defoliation and internode shortening were observed in production fields in Jujuy province (Argentina). A phytoplasma was detected by PCR using the universal primer pairs P1/P7 and R16F2n/R16R2 in all the symptomatic samples analysed. The RFLP profile of PCR products, amplified with R16F2n/R16R2 primers, shows that this phytoplasma, named Argentinean Peach Yellows (ArPY), belongs to subgroup 16Sr III-B. The phylogenetic analysis of the 1244 bp 16S rDNA cloned sequence, grouped the ArPY phytoplasma into the X-disease group with a closer relationship with CFSD, PssWB and ChTDIII phytoplasmas. This is the first report of a phytoplasma infecting peach trees in Argentina.
Instituto de Patología Vegetal
Fil: Fernandez, Franco Daniel. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina
Fil: Guzman, Fabiana. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina
Fil: Curzel, Viviana. Universidad Nacional de Jujuy. Facultad de Ciencias Agrarias. Laboratorio de Diagnóstico de Enfermedades de las Plantas; Argentina
Fil: Bejarano, Noemí. Universidad Nacional de Jujuy. Facultad de Ciencias Agrarias. Laboratorio de Diagnóstico de Enfermedades de las Plantas; Argentina
Fil: Conci, Luis Rogelio. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina. Universidad Católica de Córdoba. Facultad de Agronomía y Veterinaria; Argentina
Fuente
European journal of plant pathology 135 (4) : 627–631. (April 2013)
Materia
Prunus Persica
Fitoplasmas
Durazno
Clorosis
Phytoplasmas
Peaches
Chlorosis
PCR
Molecular Characterization
16S rDNA
Yellowing
PCR-RFLP
Argentina
Nivel de accesibilidad
acceso restringido
Condiciones de uso
Repositorio
INTA Digital (INTA)
Institución
Instituto Nacional de Tecnología Agropecuaria
OAI Identificador
oai:localhost:20.500.12123/3784
Acceder
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network_name_str INTA Digital (INTA)
spelling Detection and molecular characterization of a phytoplasma affecting Prunus persica L. in Jujuy, ArgentinaFernandez, Franco DanielGuzman, FabianaCurzel, VivianaBejarano, NoemíConci, Luis RogelioPrunus PersicaFitoplasmasDuraznoClorosisPhytoplasmasPeachesChlorosisPCRMolecular Characterization16S rDNAYellowingPCR-RFLPArgentinaPeach (Prunus persica L.) plants with symptoms of yellowing, reddening, curling and leaf necrosis, premature defoliation and internode shortening were observed in production fields in Jujuy province (Argentina). A phytoplasma was detected by PCR using the universal primer pairs P1/P7 and R16F2n/R16R2 in all the symptomatic samples analysed. The RFLP profile of PCR products, amplified with R16F2n/R16R2 primers, shows that this phytoplasma, named Argentinean Peach Yellows (ArPY), belongs to subgroup 16Sr III-B. The phylogenetic analysis of the 1244 bp 16S rDNA cloned sequence, grouped the ArPY phytoplasma into the X-disease group with a closer relationship with CFSD, PssWB and ChTDIII phytoplasmas. This is the first report of a phytoplasma infecting peach trees in Argentina.Instituto de Patología VegetalFil: Fernandez, Franco Daniel. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; ArgentinaFil: Guzman, Fabiana. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; ArgentinaFil: Curzel, Viviana. Universidad Nacional de Jujuy. Facultad de Ciencias Agrarias. Laboratorio de Diagnóstico de Enfermedades de las Plantas; ArgentinaFil: Bejarano, Noemí. Universidad Nacional de Jujuy. Facultad de Ciencias Agrarias. Laboratorio de Diagnóstico de Enfermedades de las Plantas; ArgentinaFil: Conci, Luis Rogelio. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina. Universidad Católica de Córdoba. Facultad de Agronomía y Veterinaria; ArgentinaSpringer2018-11-05T14:09:31Z2018-11-05T14:09:31Z2013-04info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://hdl.handle.net/20.500.12123/3784https://link.springer.com/article/10.1007/s10658-012-0109-z#citeas0929-18731573-8469 (Online)https://doi.org/10.1007/s10658-012-0109-zEuropean journal of plant pathology 135 (4) : 627–631. (April 2013)reponame:INTA Digital (INTA)instname:Instituto Nacional de Tecnología Agropecuariaenginfo:eu-repo/semantics/restrictedAccess2025-09-04T09:47:38Zoai:localhost:20.500.12123/3784instacron:INTAInstitucionalhttp://repositorio.inta.gob.ar/Organismo científico-tecnológicoNo correspondehttp://repositorio.inta.gob.ar/oai/requesttripaldi.nicolas@inta.gob.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:l2025-09-04 09:47:39.75INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuariafalse
dc.title.none.fl_str_mv Detection and molecular characterization of a phytoplasma affecting Prunus persica L. in Jujuy, Argentina
title Detection and molecular characterization of a phytoplasma affecting Prunus persica L. in Jujuy, Argentina
spellingShingle Detection and molecular characterization of a phytoplasma affecting Prunus persica L. in Jujuy, Argentina
Fernandez, Franco Daniel
Prunus Persica
Fitoplasmas
Durazno
Clorosis
Phytoplasmas
Peaches
Chlorosis
PCR
Molecular Characterization
16S rDNA
Yellowing
PCR-RFLP
Argentina
title_short Detection and molecular characterization of a phytoplasma affecting Prunus persica L. in Jujuy, Argentina
title_full Detection and molecular characterization of a phytoplasma affecting Prunus persica L. in Jujuy, Argentina
title_fullStr Detection and molecular characterization of a phytoplasma affecting Prunus persica L. in Jujuy, Argentina
title_full_unstemmed Detection and molecular characterization of a phytoplasma affecting Prunus persica L. in Jujuy, Argentina
title_sort Detection and molecular characterization of a phytoplasma affecting Prunus persica L. in Jujuy, Argentina
dc.creator.none.fl_str_mv Fernandez, Franco Daniel
Guzman, Fabiana
Curzel, Viviana
Bejarano, Noemí
Conci, Luis Rogelio
author Fernandez, Franco Daniel
author_facet Fernandez, Franco Daniel
Guzman, Fabiana
Curzel, Viviana
Bejarano, Noemí
Conci, Luis Rogelio
author_role author
author2 Guzman, Fabiana
Curzel, Viviana
Bejarano, Noemí
Conci, Luis Rogelio
author2_role author
author
author
author
dc.subject.none.fl_str_mv Prunus Persica
Fitoplasmas
Durazno
Clorosis
Phytoplasmas
Peaches
Chlorosis
PCR
Molecular Characterization
16S rDNA
Yellowing
PCR-RFLP
Argentina
topic Prunus Persica
Fitoplasmas
Durazno
Clorosis
Phytoplasmas
Peaches
Chlorosis
PCR
Molecular Characterization
16S rDNA
Yellowing
PCR-RFLP
Argentina
dc.description.none.fl_txt_mv Peach (Prunus persica L.) plants with symptoms of yellowing, reddening, curling and leaf necrosis, premature defoliation and internode shortening were observed in production fields in Jujuy province (Argentina). A phytoplasma was detected by PCR using the universal primer pairs P1/P7 and R16F2n/R16R2 in all the symptomatic samples analysed. The RFLP profile of PCR products, amplified with R16F2n/R16R2 primers, shows that this phytoplasma, named Argentinean Peach Yellows (ArPY), belongs to subgroup 16Sr III-B. The phylogenetic analysis of the 1244 bp 16S rDNA cloned sequence, grouped the ArPY phytoplasma into the X-disease group with a closer relationship with CFSD, PssWB and ChTDIII phytoplasmas. This is the first report of a phytoplasma infecting peach trees in Argentina.
Instituto de Patología Vegetal
Fil: Fernandez, Franco Daniel. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina
Fil: Guzman, Fabiana. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina
Fil: Curzel, Viviana. Universidad Nacional de Jujuy. Facultad de Ciencias Agrarias. Laboratorio de Diagnóstico de Enfermedades de las Plantas; Argentina
Fil: Bejarano, Noemí. Universidad Nacional de Jujuy. Facultad de Ciencias Agrarias. Laboratorio de Diagnóstico de Enfermedades de las Plantas; Argentina
Fil: Conci, Luis Rogelio. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina. Universidad Católica de Córdoba. Facultad de Agronomía y Veterinaria; Argentina
description Peach (Prunus persica L.) plants with symptoms of yellowing, reddening, curling and leaf necrosis, premature defoliation and internode shortening were observed in production fields in Jujuy province (Argentina). A phytoplasma was detected by PCR using the universal primer pairs P1/P7 and R16F2n/R16R2 in all the symptomatic samples analysed. The RFLP profile of PCR products, amplified with R16F2n/R16R2 primers, shows that this phytoplasma, named Argentinean Peach Yellows (ArPY), belongs to subgroup 16Sr III-B. The phylogenetic analysis of the 1244 bp 16S rDNA cloned sequence, grouped the ArPY phytoplasma into the X-disease group with a closer relationship with CFSD, PssWB and ChTDIII phytoplasmas. This is the first report of a phytoplasma infecting peach trees in Argentina.
publishDate 2013
dc.date.none.fl_str_mv 2013-04
2018-11-05T14:09:31Z
2018-11-05T14:09:31Z
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/20.500.12123/3784
https://link.springer.com/article/10.1007/s10658-012-0109-z#citeas
0929-1873
1573-8469 (Online)
https://doi.org/10.1007/s10658-012-0109-z
url http://hdl.handle.net/20.500.12123/3784
https://link.springer.com/article/10.1007/s10658-012-0109-z#citeas
https://doi.org/10.1007/s10658-012-0109-z
identifier_str_mv 0929-1873
1573-8469 (Online)
dc.language.none.fl_str_mv eng
language eng
dc.rights.none.fl_str_mv info:eu-repo/semantics/restrictedAccess
eu_rights_str_mv restrictedAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Springer
publisher.none.fl_str_mv Springer
dc.source.none.fl_str_mv European journal of plant pathology 135 (4) : 627–631. (April 2013)
reponame:INTA Digital (INTA)
instname:Instituto Nacional de Tecnología Agropecuaria
reponame_str INTA Digital (INTA)
collection INTA Digital (INTA)
instname_str Instituto Nacional de Tecnología Agropecuaria
repository.name.fl_str_mv INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuaria
repository.mail.fl_str_mv tripaldi.nicolas@inta.gob.ar
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score 12.623145

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