Characterization and validation of olive FAD and SAD gene families: expression analysis in diferent tissues and during fruit development

Autores
Contreras Valentín, Ana Cibeles; Mariotti, Roberto; Mousavi, S.; Baldoni, L.; Guerrero, C.; Roka, L.; Cultrera, N.; Pierantozzi, Pierluigi; Maestri, Damián; Gentili, Luciana; Tivani, Martín; Torres, Myriam Mariela
Año de publicación
2020
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Stearoyl-ACP desaturases (SADs) and fatty acid desaturases (FADs) play a critical role in plant lipid metabolism and also afect oil fatty acid composition introducing double bonds into the hydrocarbon chains to produce unsaturated fatty acids. In the present study, the genomic sequences of three SAD and three FAD candidate genes were characterized in olive and their expression was evaluated in diferent plant tissues. OeSAD genes corresponded to olive SAD1 and SAD2 and to a newly identifed OeSAD4, sharing the conserved protein structure with other plant species. On the other hand, the full-length genomic sequences of two microsomal OeFAD genes (FAD2-1 and FAD2-2) and the plastidial FAD6, were released. When the level of expression was tested on diferent tissues of cv. Leccino, OeSAD1 and OeSAD2 were mainly expressed in the fruits, while OeFAD genes showed the lowest expression in this tissue. The mRNA profling of all genes was directly studied in fruits of Leccino and Coratina cultivars during fruit development. In both genotypes, the expression level of OeSAD1and OeSAD2 had the highest value during and after the pit-hardening period, when oil accumulation in fruit mesocarp is intensively increasing. Furthermore, the expression level of both OeFAD2 genes, which were the main candidates for oleic acid desaturation, were almost negligible during fruit ripening. These results have made possible to defne candidate genes of the machinery regulation of fatty acid composition in olive oil, providing information on their sequence, gene structure and chromosomal location.
EEA San Juan
Fil: Contreras Valetín, Ana Cibeles. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria San Juan; Argentina.
Fil: Contreras Valetín, Ana Cibeles. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Mariotti, Roberto. Consiglio Nazionale delle Ricerche. Institute of Biosciences and Bioresources; Italia
Fil: Mousavi, S. Consiglio Nazionale delle Ricerche. Institute of Biosciences and Bioresources; Italia
Fil: Baldoni, L. Consiglio Nazionale delle Ricerche. Institute of Biosciences and Bioresources; Italia
Fil: Guerrero, C. University of Malaga. Science Faculty. Department of Molecular Biology and Biochemistry ; España
Fil: Roka, L. Agricultural University of Athens. Department of Biotechnology; Grecia
Fil: Cultrera, N. Consiglio Nazionale delle Ricerche. Institute of Biosciences and Bioresources; Italia
Fil: Pierantozzi, Pierluigi. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria San Juan; Argentina.
Fil: Pierantozzi, Pierluigi. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Maestri, Damián. Universidad Nacional de Córdoba. Instituto Multidisciplinario de Biología Vegetal; Argentina
Fil: Maestri, Damián. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Gentili, Luciana. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria San Juan; Argentina.
Fil: Gentili, Luciana. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Tivani, Martín. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria San Juan; Argentina.
Fil: Tivani, Martín. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Torres, Myriam Mariela. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria San Juan; Argentina.
Fil: Torres, Myriam Mariela. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fuente
Molecular Biology Reports 47 (6) : 4345-4355 (May 2020)
Materia
Olea europaea
Expresión Génica
Mesocarpio
Gene Expression
Mesocarp
Genetics
Genética
Olivo
Nivel de accesibilidad
acceso restringido
Condiciones de uso
Repositorio
INTA Digital (INTA)
Institución
Instituto Nacional de Tecnología Agropecuaria
OAI Identificador
oai:localhost:20.500.12123/9283
Acceder
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oai_identifier_str oai:localhost:20.500.12123/9283
network_acronym_str INTADig
repository_id_str l
network_name_str INTA Digital (INTA)
spelling Characterization and validation of olive FAD and SAD gene families: expression analysis in diferent tissues and during fruit developmentContreras Valentín, Ana CibelesMariotti, RobertoMousavi, S.Baldoni, L.Guerrero, C.Roka, L.Cultrera, N.Pierantozzi, PierluigiMaestri, DamiánGentili, LucianaTivani, MartínTorres, Myriam MarielaOlea europaeaExpresión GénicaMesocarpioGene ExpressionMesocarpGeneticsGenéticaOlivoStearoyl-ACP desaturases (SADs) and fatty acid desaturases (FADs) play a critical role in plant lipid metabolism and also afect oil fatty acid composition introducing double bonds into the hydrocarbon chains to produce unsaturated fatty acids. In the present study, the genomic sequences of three SAD and three FAD candidate genes were characterized in olive and their expression was evaluated in diferent plant tissues. OeSAD genes corresponded to olive SAD1 and SAD2 and to a newly identifed OeSAD4, sharing the conserved protein structure with other plant species. On the other hand, the full-length genomic sequences of two microsomal OeFAD genes (FAD2-1 and FAD2-2) and the plastidial FAD6, were released. When the level of expression was tested on diferent tissues of cv. Leccino, OeSAD1 and OeSAD2 were mainly expressed in the fruits, while OeFAD genes showed the lowest expression in this tissue. The mRNA profling of all genes was directly studied in fruits of Leccino and Coratina cultivars during fruit development. In both genotypes, the expression level of OeSAD1and OeSAD2 had the highest value during and after the pit-hardening period, when oil accumulation in fruit mesocarp is intensively increasing. Furthermore, the expression level of both OeFAD2 genes, which were the main candidates for oleic acid desaturation, were almost negligible during fruit ripening. These results have made possible to defne candidate genes of the machinery regulation of fatty acid composition in olive oil, providing information on their sequence, gene structure and chromosomal location.EEA San JuanFil: Contreras Valetín, Ana Cibeles. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria San Juan; Argentina.Fil: Contreras Valetín, Ana Cibeles. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Mariotti, Roberto. Consiglio Nazionale delle Ricerche. Institute of Biosciences and Bioresources; ItaliaFil: Mousavi, S. Consiglio Nazionale delle Ricerche. Institute of Biosciences and Bioresources; ItaliaFil: Baldoni, L. Consiglio Nazionale delle Ricerche. Institute of Biosciences and Bioresources; ItaliaFil: Guerrero, C. University of Malaga. Science Faculty. Department of Molecular Biology and Biochemistry ; EspañaFil: Roka, L. Agricultural University of Athens. Department of Biotechnology; GreciaFil: Cultrera, N. Consiglio Nazionale delle Ricerche. Institute of Biosciences and Bioresources; ItaliaFil: Pierantozzi, Pierluigi. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria San Juan; Argentina.Fil: Pierantozzi, Pierluigi. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Maestri, Damián. Universidad Nacional de Córdoba. Instituto Multidisciplinario de Biología Vegetal; ArgentinaFil: Maestri, Damián. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Gentili, Luciana. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria San Juan; Argentina.Fil: Gentili, Luciana. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Tivani, Martín. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria San Juan; Argentina.Fil: Tivani, Martín. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Torres, Myriam Mariela. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria San Juan; Argentina.Fil: Torres, Myriam Mariela. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaSpringer2021-05-05T18:56:59Z2021-05-05T18:56:59Z2020-05-28info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://hdl.handle.net/20.500.12123/9283https://link.springer.com/article/10.1007/s11033-020-05554-90301-48511573-4978https://doi.org/10.1007/s11033-020-05554-9Molecular Biology Reports 47 (6) : 4345-4355 (May 2020)reponame:INTA Digital (INTA)instname:Instituto Nacional de Tecnología Agropecuariaenginfo:eu-repo/semantics/restrictedAccess2025-10-16T09:30:04Zoai:localhost:20.500.12123/9283instacron:INTAInstitucionalhttp://repositorio.inta.gob.ar/Organismo científico-tecnológicoNo correspondehttp://repositorio.inta.gob.ar/oai/requesttripaldi.nicolas@inta.gob.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:l2025-10-16 09:30:05.301INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuariafalse
dc.title.none.fl_str_mv Characterization and validation of olive FAD and SAD gene families: expression analysis in diferent tissues and during fruit development
title Characterization and validation of olive FAD and SAD gene families: expression analysis in diferent tissues and during fruit development
spellingShingle Characterization and validation of olive FAD and SAD gene families: expression analysis in diferent tissues and during fruit development
Contreras Valentín, Ana Cibeles
Olea europaea
Expresión Génica
Mesocarpio
Gene Expression
Mesocarp
Genetics
Genética
Olivo
title_short Characterization and validation of olive FAD and SAD gene families: expression analysis in diferent tissues and during fruit development
title_full Characterization and validation of olive FAD and SAD gene families: expression analysis in diferent tissues and during fruit development
title_fullStr Characterization and validation of olive FAD and SAD gene families: expression analysis in diferent tissues and during fruit development
title_full_unstemmed Characterization and validation of olive FAD and SAD gene families: expression analysis in diferent tissues and during fruit development
title_sort Characterization and validation of olive FAD and SAD gene families: expression analysis in diferent tissues and during fruit development
dc.creator.none.fl_str_mv Contreras Valentín, Ana Cibeles
Mariotti, Roberto
Mousavi, S.
Baldoni, L.
Guerrero, C.
Roka, L.
Cultrera, N.
Pierantozzi, Pierluigi
Maestri, Damián
Gentili, Luciana
Tivani, Martín
Torres, Myriam Mariela
author Contreras Valentín, Ana Cibeles
author_facet Contreras Valentín, Ana Cibeles
Mariotti, Roberto
Mousavi, S.
Baldoni, L.
Guerrero, C.
Roka, L.
Cultrera, N.
Pierantozzi, Pierluigi
Maestri, Damián
Gentili, Luciana
Tivani, Martín
Torres, Myriam Mariela
author_role author
author2 Mariotti, Roberto
Mousavi, S.
Baldoni, L.
Guerrero, C.
Roka, L.
Cultrera, N.
Pierantozzi, Pierluigi
Maestri, Damián
Gentili, Luciana
Tivani, Martín
Torres, Myriam Mariela
author2_role author
author
author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Olea europaea
Expresión Génica
Mesocarpio
Gene Expression
Mesocarp
Genetics
Genética
Olivo
topic Olea europaea
Expresión Génica
Mesocarpio
Gene Expression
Mesocarp
Genetics
Genética
Olivo
dc.description.none.fl_txt_mv Stearoyl-ACP desaturases (SADs) and fatty acid desaturases (FADs) play a critical role in plant lipid metabolism and also afect oil fatty acid composition introducing double bonds into the hydrocarbon chains to produce unsaturated fatty acids. In the present study, the genomic sequences of three SAD and three FAD candidate genes were characterized in olive and their expression was evaluated in diferent plant tissues. OeSAD genes corresponded to olive SAD1 and SAD2 and to a newly identifed OeSAD4, sharing the conserved protein structure with other plant species. On the other hand, the full-length genomic sequences of two microsomal OeFAD genes (FAD2-1 and FAD2-2) and the plastidial FAD6, were released. When the level of expression was tested on diferent tissues of cv. Leccino, OeSAD1 and OeSAD2 were mainly expressed in the fruits, while OeFAD genes showed the lowest expression in this tissue. The mRNA profling of all genes was directly studied in fruits of Leccino and Coratina cultivars during fruit development. In both genotypes, the expression level of OeSAD1and OeSAD2 had the highest value during and after the pit-hardening period, when oil accumulation in fruit mesocarp is intensively increasing. Furthermore, the expression level of both OeFAD2 genes, which were the main candidates for oleic acid desaturation, were almost negligible during fruit ripening. These results have made possible to defne candidate genes of the machinery regulation of fatty acid composition in olive oil, providing information on their sequence, gene structure and chromosomal location.
EEA San Juan
Fil: Contreras Valetín, Ana Cibeles. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria San Juan; Argentina.
Fil: Contreras Valetín, Ana Cibeles. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Mariotti, Roberto. Consiglio Nazionale delle Ricerche. Institute of Biosciences and Bioresources; Italia
Fil: Mousavi, S. Consiglio Nazionale delle Ricerche. Institute of Biosciences and Bioresources; Italia
Fil: Baldoni, L. Consiglio Nazionale delle Ricerche. Institute of Biosciences and Bioresources; Italia
Fil: Guerrero, C. University of Malaga. Science Faculty. Department of Molecular Biology and Biochemistry ; España
Fil: Roka, L. Agricultural University of Athens. Department of Biotechnology; Grecia
Fil: Cultrera, N. Consiglio Nazionale delle Ricerche. Institute of Biosciences and Bioresources; Italia
Fil: Pierantozzi, Pierluigi. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria San Juan; Argentina.
Fil: Pierantozzi, Pierluigi. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Maestri, Damián. Universidad Nacional de Córdoba. Instituto Multidisciplinario de Biología Vegetal; Argentina
Fil: Maestri, Damián. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Gentili, Luciana. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria San Juan; Argentina.
Fil: Gentili, Luciana. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Tivani, Martín. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria San Juan; Argentina.
Fil: Tivani, Martín. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Torres, Myriam Mariela. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria San Juan; Argentina.
Fil: Torres, Myriam Mariela. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
description Stearoyl-ACP desaturases (SADs) and fatty acid desaturases (FADs) play a critical role in plant lipid metabolism and also afect oil fatty acid composition introducing double bonds into the hydrocarbon chains to produce unsaturated fatty acids. In the present study, the genomic sequences of three SAD and three FAD candidate genes were characterized in olive and their expression was evaluated in diferent plant tissues. OeSAD genes corresponded to olive SAD1 and SAD2 and to a newly identifed OeSAD4, sharing the conserved protein structure with other plant species. On the other hand, the full-length genomic sequences of two microsomal OeFAD genes (FAD2-1 and FAD2-2) and the plastidial FAD6, were released. When the level of expression was tested on diferent tissues of cv. Leccino, OeSAD1 and OeSAD2 were mainly expressed in the fruits, while OeFAD genes showed the lowest expression in this tissue. The mRNA profling of all genes was directly studied in fruits of Leccino and Coratina cultivars during fruit development. In both genotypes, the expression level of OeSAD1and OeSAD2 had the highest value during and after the pit-hardening period, when oil accumulation in fruit mesocarp is intensively increasing. Furthermore, the expression level of both OeFAD2 genes, which were the main candidates for oleic acid desaturation, were almost negligible during fruit ripening. These results have made possible to defne candidate genes of the machinery regulation of fatty acid composition in olive oil, providing information on their sequence, gene structure and chromosomal location.
publishDate 2020
dc.date.none.fl_str_mv 2020-05-28
2021-05-05T18:56:59Z
2021-05-05T18:56:59Z
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/20.500.12123/9283
https://link.springer.com/article/10.1007/s11033-020-05554-9
0301-4851
1573-4978
https://doi.org/10.1007/s11033-020-05554-9
url http://hdl.handle.net/20.500.12123/9283
https://link.springer.com/article/10.1007/s11033-020-05554-9
https://doi.org/10.1007/s11033-020-05554-9
identifier_str_mv 0301-4851
1573-4978
dc.language.none.fl_str_mv eng
language eng
dc.rights.none.fl_str_mv info:eu-repo/semantics/restrictedAccess
eu_rights_str_mv restrictedAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Springer
publisher.none.fl_str_mv Springer
dc.source.none.fl_str_mv Molecular Biology Reports 47 (6) : 4345-4355 (May 2020)
reponame:INTA Digital (INTA)
instname:Instituto Nacional de Tecnología Agropecuaria
reponame_str INTA Digital (INTA)
collection INTA Digital (INTA)
instname_str Instituto Nacional de Tecnología Agropecuaria
repository.name.fl_str_mv INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuaria
repository.mail.fl_str_mv tripaldi.nicolas@inta.gob.ar
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