Comparison of phenotypic tests for detecting penicillin G resistance with presence of blaZ gene in Staphylococcus aureus isolated from bovine intramammary infections

Autores
Russi, Norma B.; Maito, Julia; Dieser, Silvana Andrea; Renna, Maria Sol; Signorini, Marcelo; Camussone, Cecilia; Neder, Veronica Elizabeth; Pol, Martín; Tirante, Liliana; Odierno, Liliana Mónica; Calvinho, Luis Fernando
Año de publicación
2015
Idioma
inglés
Tipo de recurso
artículo
Estado
versión aceptada
Descripción
Few studies have described the relationship between genotypic and phenotypic methods for detecting penicillin resistance in Staphylococcus aureus isolated from bovine intramammary infection (IMI). Six phenotypic methods for penicillinase detection were compared with a genotypic method testing the presence of the β-lactamase gene blaZ in Staph. aureus (n = 150) isolated from bovine IMI. Highest sensitivities and specificities were observed for disk diffusion (DD) (93 and 97·4%), minimum inhibitory concentration (MIC) (90·3 and 97·4%), Cefinase™ (85·9 and 97·4%) and Diatabs™ (85·7 and 98·7%). The estimated cut-off points estimated in the present study can be considered close to the ones indicated by CLSI (2013). The molecular detection of blaZ gene is the only method that may indicate the real or potential capacity of producing β-lactamase in Staph. aureus. Considering that from a clinical standpoint a false negative result from a phenotypic test is the most unfavourable situation, a combination of standard DD with Diatabs™ or Cefinase™ should be performed by routine mastitis laboratories to minimise false negative results.
EEA Rafaela
Fil: Russi, Norma B.. Universidad Nacional del Litoral. Facultad de Ciencias Veterinarias; Argentina
Fil: Maito, Julia. Lactodiagnóstico Sur; Argentina
Fil: Dieser, Silvana Andrea. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas, Fisicoquímicas y Naturales; Argentina
Fil: Renna, Maria Sol. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Ciencias Veterinarias del Litoral. Universidad Nacional del Litoral. Facultad de Ciencias Veterinarias. Instituto de Ciencias Veterinarias del Litoral; Argentina
Fil: Signorini, Marcelo. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rafaela; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Camussone, Cecilia. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rafaela; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Neder, Veronica Elizabeth. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rafaela; Argentina
Fil: Pol, Martín. Lactodiagnóstico Sur; Argentina
Fil: Tirante, Liliana. Lactodiagnóstico Sur; Argentina
Fil: Odierno, Liliana Mónica. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas, Fisicoquímicas y Naturales. Departamento de Microbiología e Inmunología; Argentina
Fil: Calvinho, Luis Fernando. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rafaela; Argentina. Universidad Nacional del Litoral. Facultad de Ciencias Veterinarias; Argentina
Fuente
Journal of Dairy Research 82 (3) : 317-321 (August 2015)
Materia
Staphylococcus aureus
Enfermedades de los Animales
Ganado Bovino
Mastítis Bovina
Penicilina
Resistencia a los Antibióticos
Animal Diseases
Cattle
Bovine Mastitis
Penicillins
Resistance to Antibiotics
Nivel de accesibilidad
acceso abierto
Condiciones de uso
http://creativecommons.org/licenses/by-nc-sa/4.0/
Repositorio
INTA Digital (INTA)
Institución
Instituto Nacional de Tecnología Agropecuaria
OAI Identificador
oai:localhost:20.500.12123/2817
Acceder
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oai_identifier_str oai:localhost:20.500.12123/2817
network_acronym_str INTADig
repository_id_str l
network_name_str INTA Digital (INTA)
spelling Comparison of phenotypic tests for detecting penicillin G resistance with presence of blaZ gene in Staphylococcus aureus isolated from bovine intramammary infectionsRussi, Norma B.Maito, JuliaDieser, Silvana AndreaRenna, Maria SolSignorini, MarceloCamussone, CeciliaNeder, Veronica ElizabethPol, MartínTirante, LilianaOdierno, Liliana MónicaCalvinho, Luis FernandoStaphylococcus aureusEnfermedades de los AnimalesGanado BovinoMastítis BovinaPenicilinaResistencia a los AntibióticosAnimal DiseasesCattleBovine MastitisPenicillinsResistance to AntibioticsFew studies have described the relationship between genotypic and phenotypic methods for detecting penicillin resistance in Staphylococcus aureus isolated from bovine intramammary infection (IMI). Six phenotypic methods for penicillinase detection were compared with a genotypic method testing the presence of the β-lactamase gene blaZ in Staph. aureus (n = 150) isolated from bovine IMI. Highest sensitivities and specificities were observed for disk diffusion (DD) (93 and 97·4%), minimum inhibitory concentration (MIC) (90·3 and 97·4%), Cefinase™ (85·9 and 97·4%) and Diatabs™ (85·7 and 98·7%). The estimated cut-off points estimated in the present study can be considered close to the ones indicated by CLSI (2013). The molecular detection of blaZ gene is the only method that may indicate the real or potential capacity of producing β-lactamase in Staph. aureus. Considering that from a clinical standpoint a false negative result from a phenotypic test is the most unfavourable situation, a combination of standard DD with Diatabs™ or Cefinase™ should be performed by routine mastitis laboratories to minimise false negative results.EEA RafaelaFil: Russi, Norma B.. Universidad Nacional del Litoral. Facultad de Ciencias Veterinarias; ArgentinaFil: Maito, Julia. Lactodiagnóstico Sur; ArgentinaFil: Dieser, Silvana Andrea. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas, Fisicoquímicas y Naturales; ArgentinaFil: Renna, Maria Sol. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Ciencias Veterinarias del Litoral. Universidad Nacional del Litoral. Facultad de Ciencias Veterinarias. Instituto de Ciencias Veterinarias del Litoral; ArgentinaFil: Signorini, Marcelo. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rafaela; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Camussone, Cecilia. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rafaela; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Neder, Veronica Elizabeth. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rafaela; ArgentinaFil: Pol, Martín. Lactodiagnóstico Sur; ArgentinaFil: Tirante, Liliana. Lactodiagnóstico Sur; ArgentinaFil: Odierno, Liliana Mónica. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas, Fisicoquímicas y Naturales. Departamento de Microbiología e Inmunología; ArgentinaFil: Calvinho, Luis Fernando. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rafaela; Argentina. Universidad Nacional del Litoral. Facultad de Ciencias Veterinarias; Argentina2018-07-18T15:56:03Z2018-07-18T15:56:03Z2015-08info:eu-repo/semantics/articleinfo:eu-repo/semantics/acceptedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttps://www.cambridge.org/core/journals/journal-of-dairy-research/article/comparison-of-phenotypic-tests-for-detecting-penicillin-g-resistance-with-presence-of-blaz-gene-in-staphylococcus-aureus-isolated-from-bovine-intramammary-infections/8AEBB8F7BD83F306EE0BF08095AFBCAChttp://hdl.handle.net/20.500.12123/28170022-02991469-7629https://doi.org/10.1017/S0022029915000242Journal of Dairy Research 82 (3) : 317-321 (August 2015)reponame:INTA Digital (INTA)instname:Instituto Nacional de Tecnología Agropecuariaenginfo:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by-nc-sa/4.0/Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)2025-09-04T09:47:21Zoai:localhost:20.500.12123/2817instacron:INTAInstitucionalhttp://repositorio.inta.gob.ar/Organismo científico-tecnológicoNo correspondehttp://repositorio.inta.gob.ar/oai/requesttripaldi.nicolas@inta.gob.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:l2025-09-04 09:47:22.385INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuariafalse
dc.title.none.fl_str_mv Comparison of phenotypic tests for detecting penicillin G resistance with presence of blaZ gene in Staphylococcus aureus isolated from bovine intramammary infections
title Comparison of phenotypic tests for detecting penicillin G resistance with presence of blaZ gene in Staphylococcus aureus isolated from bovine intramammary infections
spellingShingle Comparison of phenotypic tests for detecting penicillin G resistance with presence of blaZ gene in Staphylococcus aureus isolated from bovine intramammary infections
Russi, Norma B.
Staphylococcus aureus
Enfermedades de los Animales
Ganado Bovino
Mastítis Bovina
Penicilina
Resistencia a los Antibióticos
Animal Diseases
Cattle
Bovine Mastitis
Penicillins
Resistance to Antibiotics
title_short Comparison of phenotypic tests for detecting penicillin G resistance with presence of blaZ gene in Staphylococcus aureus isolated from bovine intramammary infections
title_full Comparison of phenotypic tests for detecting penicillin G resistance with presence of blaZ gene in Staphylococcus aureus isolated from bovine intramammary infections
title_fullStr Comparison of phenotypic tests for detecting penicillin G resistance with presence of blaZ gene in Staphylococcus aureus isolated from bovine intramammary infections
title_full_unstemmed Comparison of phenotypic tests for detecting penicillin G resistance with presence of blaZ gene in Staphylococcus aureus isolated from bovine intramammary infections
title_sort Comparison of phenotypic tests for detecting penicillin G resistance with presence of blaZ gene in Staphylococcus aureus isolated from bovine intramammary infections
dc.creator.none.fl_str_mv Russi, Norma B.
Maito, Julia
Dieser, Silvana Andrea
Renna, Maria Sol
Signorini, Marcelo
Camussone, Cecilia
Neder, Veronica Elizabeth
Pol, Martín
Tirante, Liliana
Odierno, Liliana Mónica
Calvinho, Luis Fernando
author Russi, Norma B.
author_facet Russi, Norma B.
Maito, Julia
Dieser, Silvana Andrea
Renna, Maria Sol
Signorini, Marcelo
Camussone, Cecilia
Neder, Veronica Elizabeth
Pol, Martín
Tirante, Liliana
Odierno, Liliana Mónica
Calvinho, Luis Fernando
author_role author
author2 Maito, Julia
Dieser, Silvana Andrea
Renna, Maria Sol
Signorini, Marcelo
Camussone, Cecilia
Neder, Veronica Elizabeth
Pol, Martín
Tirante, Liliana
Odierno, Liliana Mónica
Calvinho, Luis Fernando
author2_role author
author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Staphylococcus aureus
Enfermedades de los Animales
Ganado Bovino
Mastítis Bovina
Penicilina
Resistencia a los Antibióticos
Animal Diseases
Cattle
Bovine Mastitis
Penicillins
Resistance to Antibiotics
topic Staphylococcus aureus
Enfermedades de los Animales
Ganado Bovino
Mastítis Bovina
Penicilina
Resistencia a los Antibióticos
Animal Diseases
Cattle
Bovine Mastitis
Penicillins
Resistance to Antibiotics
dc.description.none.fl_txt_mv Few studies have described the relationship between genotypic and phenotypic methods for detecting penicillin resistance in Staphylococcus aureus isolated from bovine intramammary infection (IMI). Six phenotypic methods for penicillinase detection were compared with a genotypic method testing the presence of the β-lactamase gene blaZ in Staph. aureus (n = 150) isolated from bovine IMI. Highest sensitivities and specificities were observed for disk diffusion (DD) (93 and 97·4%), minimum inhibitory concentration (MIC) (90·3 and 97·4%), Cefinase™ (85·9 and 97·4%) and Diatabs™ (85·7 and 98·7%). The estimated cut-off points estimated in the present study can be considered close to the ones indicated by CLSI (2013). The molecular detection of blaZ gene is the only method that may indicate the real or potential capacity of producing β-lactamase in Staph. aureus. Considering that from a clinical standpoint a false negative result from a phenotypic test is the most unfavourable situation, a combination of standard DD with Diatabs™ or Cefinase™ should be performed by routine mastitis laboratories to minimise false negative results.
EEA Rafaela
Fil: Russi, Norma B.. Universidad Nacional del Litoral. Facultad de Ciencias Veterinarias; Argentina
Fil: Maito, Julia. Lactodiagnóstico Sur; Argentina
Fil: Dieser, Silvana Andrea. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas, Fisicoquímicas y Naturales; Argentina
Fil: Renna, Maria Sol. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Ciencias Veterinarias del Litoral. Universidad Nacional del Litoral. Facultad de Ciencias Veterinarias. Instituto de Ciencias Veterinarias del Litoral; Argentina
Fil: Signorini, Marcelo. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rafaela; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Camussone, Cecilia. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rafaela; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Neder, Veronica Elizabeth. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rafaela; Argentina
Fil: Pol, Martín. Lactodiagnóstico Sur; Argentina
Fil: Tirante, Liliana. Lactodiagnóstico Sur; Argentina
Fil: Odierno, Liliana Mónica. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas, Fisicoquímicas y Naturales. Departamento de Microbiología e Inmunología; Argentina
Fil: Calvinho, Luis Fernando. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rafaela; Argentina. Universidad Nacional del Litoral. Facultad de Ciencias Veterinarias; Argentina
description Few studies have described the relationship between genotypic and phenotypic methods for detecting penicillin resistance in Staphylococcus aureus isolated from bovine intramammary infection (IMI). Six phenotypic methods for penicillinase detection were compared with a genotypic method testing the presence of the β-lactamase gene blaZ in Staph. aureus (n = 150) isolated from bovine IMI. Highest sensitivities and specificities were observed for disk diffusion (DD) (93 and 97·4%), minimum inhibitory concentration (MIC) (90·3 and 97·4%), Cefinase™ (85·9 and 97·4%) and Diatabs™ (85·7 and 98·7%). The estimated cut-off points estimated in the present study can be considered close to the ones indicated by CLSI (2013). The molecular detection of blaZ gene is the only method that may indicate the real or potential capacity of producing β-lactamase in Staph. aureus. Considering that from a clinical standpoint a false negative result from a phenotypic test is the most unfavourable situation, a combination of standard DD with Diatabs™ or Cefinase™ should be performed by routine mastitis laboratories to minimise false negative results.
publishDate 2015
dc.date.none.fl_str_mv 2015-08
2018-07-18T15:56:03Z
2018-07-18T15:56:03Z
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/acceptedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str acceptedVersion
dc.identifier.none.fl_str_mv https://www.cambridge.org/core/journals/journal-of-dairy-research/article/comparison-of-phenotypic-tests-for-detecting-penicillin-g-resistance-with-presence-of-blaz-gene-in-staphylococcus-aureus-isolated-from-bovine-intramammary-infections/8AEBB8F7BD83F306EE0BF08095AFBCAC
http://hdl.handle.net/20.500.12123/2817
0022-0299
1469-7629
https://doi.org/10.1017/S0022029915000242
url https://www.cambridge.org/core/journals/journal-of-dairy-research/article/comparison-of-phenotypic-tests-for-detecting-penicillin-g-resistance-with-presence-of-blaz-gene-in-staphylococcus-aureus-isolated-from-bovine-intramammary-infections/8AEBB8F7BD83F306EE0BF08095AFBCAC
http://hdl.handle.net/20.500.12123/2817
https://doi.org/10.1017/S0022029915000242
identifier_str_mv 0022-0299
1469-7629
dc.language.none.fl_str_mv eng
language eng
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
eu_rights_str_mv openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv Journal of Dairy Research 82 (3) : 317-321 (August 2015)
reponame:INTA Digital (INTA)
instname:Instituto Nacional de Tecnología Agropecuaria
reponame_str INTA Digital (INTA)
collection INTA Digital (INTA)
instname_str Instituto Nacional de Tecnología Agropecuaria
repository.name.fl_str_mv INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuaria
repository.mail.fl_str_mv tripaldi.nicolas@inta.gob.ar
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