Enterohemorrhagic Escherichia coli O157:H7 antigens produced in transgenic lettuce effective as an oral vaccine in mice

Autores
Su, Yun‑Lin; Larzabal, Mariano; Song, Huan; Cheng, Tianfang; Wang, Yufang; Smith, Libia Yael; Cataldi, Angel Adrian; Ow, David W.
Año de publicación
2023
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Enterohemorrhagic Escherichia coli (EHEC) O157:H7 is a food-borne pathogen where ruminant farm animals, mainly bovine, serve as reservoirs. Bovine vaccination has been used to prevent disease outbreaks, and the current method relies on vaccines subcutaneously injected three times per year. Since EHEC O157:H7 colonizes mucosal surfaces, an oral vaccine that produces an IgA response could be more convenient. Here, we report on oral vaccination against EHEC O157:H7 in mice orally gavaged with transgenic lettuce that produces EHEC O157:H7 antigens EspB and γ-intimin C280. Younger leaves accumulated a higher concentration of antigens; and in unexpanded leaves of 30-day-old T2 plants, EspB and γ-intimin C280 were up to 32 and 51 μg/g fresh weight, respectively. Mice orally gavaged with lettuce powders containing < 3 µg antigens for 6 days showed a mucosal immune response with reduced colonization of EHEC O157:H7. This suggests that the transgenic lettuce has potential to be used for bovine vaccination. To promote the biosafety of crop plants producing medically relevant proteins, recombination sites were built into our transgenic lines that would permit optional marker removal by Cre-lox recombination, as well as transgene deletion in pollen by CinH-RS2 recombination. The ability to upgrade the transgenic lettuce by stacking additional antigen genes or replacing older genes with newer versions would also be possible through the combined use of Bxb-att and Cre-lox recombination systems.
Instituto de Biotecnología
Fil: Su, Yun‑Lin. Chinese Academy of Sciences. South China Botanical Garden. Guangdong Provincial Key Laboratory of Applied Botany. Key Laboratory of South China Agricultural Plant Molecular Analysis and Genetic Improvement. Plant Gene Engineering Center; República Popular China
Fil: Larzabal, Mariano. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular (IABIMO); Argentina
Fil: Larzabal, Mariano. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Song, Huan. Chinese Academy of Sciences. South China Botanical Garden. Guangdong Provincial Key Laboratory of Applied Botany. Key Laboratory of South China Agricultural Plant Molecular Analysis and Genetic Improvement. Plant Gene Engineering Center; República Popular China
Fil: Cheng, Tianfang. Chinese Academy of Sciences. South China Botanical Garden. Guangdong Provincial Key Laboratory of Applied Botany. Key Laboratory of South China Agricultural Plant Molecular Analysis and Genetic Improvement. Plant Gene Engineering Center; República Popular China
Fil: Wang, Yufang. Chinese Academy of Sciences. South China Botanical Garden. Guangdong Provincial Key Laboratory of Applied Botany. Key Laboratory of South China Agricultural Plant Molecular Analysis and Genetic Improvement. Plant Gene Engineering Center; República Popular China
Fil: Smith, Libia Yael. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular (IABIMO); Argentina
Fil: Smith, Libia Yael. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Cataldi, Angel Adrian. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular (IABIMO); Argentina
Fil: Cataldi, Angel Adrian. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Ow, David W. Chinese Academy of Sciences. South China Botanical Garden. Guangdong Provincial Key Laboratory of Applied Botany. Key Laboratory of South China Agricultural Plant Molecular Analysis and Genetic Improvement. Plant Gene Engineering Center; República Popular China
Fuente
Theoretical and Applied Genetics 136 (10) : 214 (Octubre 2023)
Materia
Escherichia coli
Antígenos
Lechugas
Vacuna
Ratón
Antigens
Lettuces
Vaccines
Mice
Transgenes
Nivel de accesibilidad
acceso restringido
Condiciones de uso
http://creativecommons.org/licenses/by-nc-sa/4.0/
Repositorio
INTA Digital (INTA)
Institución
Instituto Nacional de Tecnología Agropecuaria
OAI Identificador
oai:localhost:20.500.12123/15662
Acceder
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oai_identifier_str oai:localhost:20.500.12123/15662
network_acronym_str INTADig
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network_name_str INTA Digital (INTA)
spelling Enterohemorrhagic Escherichia coli O157:H7 antigens produced in transgenic lettuce effective as an oral vaccine in miceSu, Yun‑LinLarzabal, MarianoSong, HuanCheng, TianfangWang, YufangSmith, Libia YaelCataldi, Angel AdrianOw, David W.Escherichia coliAntígenosLechugasVacunaRatónAntigensLettucesVaccinesMiceTransgenesEnterohemorrhagic Escherichia coli (EHEC) O157:H7 is a food-borne pathogen where ruminant farm animals, mainly bovine, serve as reservoirs. Bovine vaccination has been used to prevent disease outbreaks, and the current method relies on vaccines subcutaneously injected three times per year. Since EHEC O157:H7 colonizes mucosal surfaces, an oral vaccine that produces an IgA response could be more convenient. Here, we report on oral vaccination against EHEC O157:H7 in mice orally gavaged with transgenic lettuce that produces EHEC O157:H7 antigens EspB and γ-intimin C280. Younger leaves accumulated a higher concentration of antigens; and in unexpanded leaves of 30-day-old T2 plants, EspB and γ-intimin C280 were up to 32 and 51 μg/g fresh weight, respectively. Mice orally gavaged with lettuce powders containing < 3 µg antigens for 6 days showed a mucosal immune response with reduced colonization of EHEC O157:H7. This suggests that the transgenic lettuce has potential to be used for bovine vaccination. To promote the biosafety of crop plants producing medically relevant proteins, recombination sites were built into our transgenic lines that would permit optional marker removal by Cre-lox recombination, as well as transgene deletion in pollen by CinH-RS2 recombination. The ability to upgrade the transgenic lettuce by stacking additional antigen genes or replacing older genes with newer versions would also be possible through the combined use of Bxb-att and Cre-lox recombination systems.Instituto de BiotecnologíaFil: Su, Yun‑Lin. Chinese Academy of Sciences. South China Botanical Garden. Guangdong Provincial Key Laboratory of Applied Botany. Key Laboratory of South China Agricultural Plant Molecular Analysis and Genetic Improvement. Plant Gene Engineering Center; República Popular ChinaFil: Larzabal, Mariano. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular (IABIMO); ArgentinaFil: Larzabal, Mariano. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Song, Huan. Chinese Academy of Sciences. South China Botanical Garden. Guangdong Provincial Key Laboratory of Applied Botany. Key Laboratory of South China Agricultural Plant Molecular Analysis and Genetic Improvement. Plant Gene Engineering Center; República Popular ChinaFil: Cheng, Tianfang. Chinese Academy of Sciences. South China Botanical Garden. Guangdong Provincial Key Laboratory of Applied Botany. Key Laboratory of South China Agricultural Plant Molecular Analysis and Genetic Improvement. Plant Gene Engineering Center; República Popular ChinaFil: Wang, Yufang. Chinese Academy of Sciences. South China Botanical Garden. Guangdong Provincial Key Laboratory of Applied Botany. Key Laboratory of South China Agricultural Plant Molecular Analysis and Genetic Improvement. Plant Gene Engineering Center; República Popular ChinaFil: Smith, Libia Yael. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular (IABIMO); ArgentinaFil: Smith, Libia Yael. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Cataldi, Angel Adrian. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular (IABIMO); ArgentinaFil: Cataldi, Angel Adrian. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Ow, David W. Chinese Academy of Sciences. South China Botanical Garden. Guangdong Provincial Key Laboratory of Applied Botany. Key Laboratory of South China Agricultural Plant Molecular Analysis and Genetic Improvement. Plant Gene Engineering Center; República Popular ChinaSpringer2023-10-24T15:00:03Z2023-10-24T15:00:03Z2023-10info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://hdl.handle.net/20.500.12123/15662https://link.springer.com/article/10.1007/s00122-023-04460-51432-2242https://doi.org/10.1007/s00122-023-04460-5Theoretical and Applied Genetics 136 (10) : 214 (Octubre 2023)reponame:INTA Digital (INTA)instname:Instituto Nacional de Tecnología Agropecuariaenginfo:eu-repo/semantics/restrictedAccesshttp://creativecommons.org/licenses/by-nc-sa/4.0/Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)2025-10-16T09:31:20Zoai:localhost:20.500.12123/15662instacron:INTAInstitucionalhttp://repositorio.inta.gob.ar/Organismo científico-tecnológicoNo correspondehttp://repositorio.inta.gob.ar/oai/requesttripaldi.nicolas@inta.gob.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:l2025-10-16 09:31:21.169INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuariafalse
dc.title.none.fl_str_mv Enterohemorrhagic Escherichia coli O157:H7 antigens produced in transgenic lettuce effective as an oral vaccine in mice
title Enterohemorrhagic Escherichia coli O157:H7 antigens produced in transgenic lettuce effective as an oral vaccine in mice
spellingShingle Enterohemorrhagic Escherichia coli O157:H7 antigens produced in transgenic lettuce effective as an oral vaccine in mice
Su, Yun‑Lin
Escherichia coli
Antígenos
Lechugas
Vacuna
Ratón
Antigens
Lettuces
Vaccines
Mice
Transgenes
title_short Enterohemorrhagic Escherichia coli O157:H7 antigens produced in transgenic lettuce effective as an oral vaccine in mice
title_full Enterohemorrhagic Escherichia coli O157:H7 antigens produced in transgenic lettuce effective as an oral vaccine in mice
title_fullStr Enterohemorrhagic Escherichia coli O157:H7 antigens produced in transgenic lettuce effective as an oral vaccine in mice
title_full_unstemmed Enterohemorrhagic Escherichia coli O157:H7 antigens produced in transgenic lettuce effective as an oral vaccine in mice
title_sort Enterohemorrhagic Escherichia coli O157:H7 antigens produced in transgenic lettuce effective as an oral vaccine in mice
dc.creator.none.fl_str_mv Su, Yun‑Lin
Larzabal, Mariano
Song, Huan
Cheng, Tianfang
Wang, Yufang
Smith, Libia Yael
Cataldi, Angel Adrian
Ow, David W.
author Su, Yun‑Lin
author_facet Su, Yun‑Lin
Larzabal, Mariano
Song, Huan
Cheng, Tianfang
Wang, Yufang
Smith, Libia Yael
Cataldi, Angel Adrian
Ow, David W.
author_role author
author2 Larzabal, Mariano
Song, Huan
Cheng, Tianfang
Wang, Yufang
Smith, Libia Yael
Cataldi, Angel Adrian
Ow, David W.
author2_role author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Escherichia coli
Antígenos
Lechugas
Vacuna
Ratón
Antigens
Lettuces
Vaccines
Mice
Transgenes
topic Escherichia coli
Antígenos
Lechugas
Vacuna
Ratón
Antigens
Lettuces
Vaccines
Mice
Transgenes
dc.description.none.fl_txt_mv Enterohemorrhagic Escherichia coli (EHEC) O157:H7 is a food-borne pathogen where ruminant farm animals, mainly bovine, serve as reservoirs. Bovine vaccination has been used to prevent disease outbreaks, and the current method relies on vaccines subcutaneously injected three times per year. Since EHEC O157:H7 colonizes mucosal surfaces, an oral vaccine that produces an IgA response could be more convenient. Here, we report on oral vaccination against EHEC O157:H7 in mice orally gavaged with transgenic lettuce that produces EHEC O157:H7 antigens EspB and γ-intimin C280. Younger leaves accumulated a higher concentration of antigens; and in unexpanded leaves of 30-day-old T2 plants, EspB and γ-intimin C280 were up to 32 and 51 μg/g fresh weight, respectively. Mice orally gavaged with lettuce powders containing < 3 µg antigens for 6 days showed a mucosal immune response with reduced colonization of EHEC O157:H7. This suggests that the transgenic lettuce has potential to be used for bovine vaccination. To promote the biosafety of crop plants producing medically relevant proteins, recombination sites were built into our transgenic lines that would permit optional marker removal by Cre-lox recombination, as well as transgene deletion in pollen by CinH-RS2 recombination. The ability to upgrade the transgenic lettuce by stacking additional antigen genes or replacing older genes with newer versions would also be possible through the combined use of Bxb-att and Cre-lox recombination systems.
Instituto de Biotecnología
Fil: Su, Yun‑Lin. Chinese Academy of Sciences. South China Botanical Garden. Guangdong Provincial Key Laboratory of Applied Botany. Key Laboratory of South China Agricultural Plant Molecular Analysis and Genetic Improvement. Plant Gene Engineering Center; República Popular China
Fil: Larzabal, Mariano. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular (IABIMO); Argentina
Fil: Larzabal, Mariano. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Song, Huan. Chinese Academy of Sciences. South China Botanical Garden. Guangdong Provincial Key Laboratory of Applied Botany. Key Laboratory of South China Agricultural Plant Molecular Analysis and Genetic Improvement. Plant Gene Engineering Center; República Popular China
Fil: Cheng, Tianfang. Chinese Academy of Sciences. South China Botanical Garden. Guangdong Provincial Key Laboratory of Applied Botany. Key Laboratory of South China Agricultural Plant Molecular Analysis and Genetic Improvement. Plant Gene Engineering Center; República Popular China
Fil: Wang, Yufang. Chinese Academy of Sciences. South China Botanical Garden. Guangdong Provincial Key Laboratory of Applied Botany. Key Laboratory of South China Agricultural Plant Molecular Analysis and Genetic Improvement. Plant Gene Engineering Center; República Popular China
Fil: Smith, Libia Yael. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular (IABIMO); Argentina
Fil: Smith, Libia Yael. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Cataldi, Angel Adrian. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular (IABIMO); Argentina
Fil: Cataldi, Angel Adrian. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Ow, David W. Chinese Academy of Sciences. South China Botanical Garden. Guangdong Provincial Key Laboratory of Applied Botany. Key Laboratory of South China Agricultural Plant Molecular Analysis and Genetic Improvement. Plant Gene Engineering Center; República Popular China
description Enterohemorrhagic Escherichia coli (EHEC) O157:H7 is a food-borne pathogen where ruminant farm animals, mainly bovine, serve as reservoirs. Bovine vaccination has been used to prevent disease outbreaks, and the current method relies on vaccines subcutaneously injected three times per year. Since EHEC O157:H7 colonizes mucosal surfaces, an oral vaccine that produces an IgA response could be more convenient. Here, we report on oral vaccination against EHEC O157:H7 in mice orally gavaged with transgenic lettuce that produces EHEC O157:H7 antigens EspB and γ-intimin C280. Younger leaves accumulated a higher concentration of antigens; and in unexpanded leaves of 30-day-old T2 plants, EspB and γ-intimin C280 were up to 32 and 51 μg/g fresh weight, respectively. Mice orally gavaged with lettuce powders containing < 3 µg antigens for 6 days showed a mucosal immune response with reduced colonization of EHEC O157:H7. This suggests that the transgenic lettuce has potential to be used for bovine vaccination. To promote the biosafety of crop plants producing medically relevant proteins, recombination sites were built into our transgenic lines that would permit optional marker removal by Cre-lox recombination, as well as transgene deletion in pollen by CinH-RS2 recombination. The ability to upgrade the transgenic lettuce by stacking additional antigen genes or replacing older genes with newer versions would also be possible through the combined use of Bxb-att and Cre-lox recombination systems.
publishDate 2023
dc.date.none.fl_str_mv 2023-10-24T15:00:03Z
2023-10-24T15:00:03Z
2023-10
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/20.500.12123/15662
https://link.springer.com/article/10.1007/s00122-023-04460-5
1432-2242
https://doi.org/10.1007/s00122-023-04460-5
url http://hdl.handle.net/20.500.12123/15662
https://link.springer.com/article/10.1007/s00122-023-04460-5
https://doi.org/10.1007/s00122-023-04460-5
identifier_str_mv 1432-2242
dc.language.none.fl_str_mv eng
language eng
dc.rights.none.fl_str_mv info:eu-repo/semantics/restrictedAccess
http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
eu_rights_str_mv restrictedAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Springer
publisher.none.fl_str_mv Springer
dc.source.none.fl_str_mv Theoretical and Applied Genetics 136 (10) : 214 (Octubre 2023)
reponame:INTA Digital (INTA)
instname:Instituto Nacional de Tecnología Agropecuaria
reponame_str INTA Digital (INTA)
collection INTA Digital (INTA)
instname_str Instituto Nacional de Tecnología Agropecuaria
repository.name.fl_str_mv INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuaria
repository.mail.fl_str_mv tripaldi.nicolas@inta.gob.ar
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score 12.712165

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