Enterohemorrhagic Escherichia coli O157:H7 antigens produced in transgenic lettuce effective as an oral vaccine in mice
- Autores
- Su, Yun‑Lin; Larzabal, Mariano; Song, Huan; Cheng, Tianfang; Wang, Yufang; Smith, Libia Yael; Cataldi, Angel Adrian; Ow, David W.
- Año de publicación
- 2023
- Idioma
- inglés
- Tipo de recurso
- artículo
- Estado
- versión publicada
- Descripción
- Enterohemorrhagic Escherichia coli (EHEC) O157:H7 is a food-borne pathogen where ruminant farm animals, mainly bovine, serve as reservoirs. Bovine vaccination has been used to prevent disease outbreaks, and the current method relies on vaccines subcutaneously injected three times per year. Since EHEC O157:H7 colonizes mucosal surfaces, an oral vaccine that produces an IgA response could be more convenient. Here, we report on oral vaccination against EHEC O157:H7 in mice orally gavaged with transgenic lettuce that produces EHEC O157:H7 antigens EspB and γ-intimin C280. Younger leaves accumulated a higher concentration of antigens; and in unexpanded leaves of 30-day-old T2 plants, EspB and γ-intimin C280 were up to 32 and 51 μg/g fresh weight, respectively. Mice orally gavaged with lettuce powders containing < 3 µg antigens for 6 days showed a mucosal immune response with reduced colonization of EHEC O157:H7. This suggests that the transgenic lettuce has potential to be used for bovine vaccination. To promote the biosafety of crop plants producing medically relevant proteins, recombination sites were built into our transgenic lines that would permit optional marker removal by Cre-lox recombination, as well as transgene deletion in pollen by CinH-RS2 recombination. The ability to upgrade the transgenic lettuce by stacking additional antigen genes or replacing older genes with newer versions would also be possible through the combined use of Bxb-att and Cre-lox recombination systems.
Instituto de Biotecnología
Fil: Su, Yun‑Lin. Chinese Academy of Sciences. South China Botanical Garden. Guangdong Provincial Key Laboratory of Applied Botany. Key Laboratory of South China Agricultural Plant Molecular Analysis and Genetic Improvement. Plant Gene Engineering Center; República Popular China
Fil: Larzabal, Mariano. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular (IABIMO); Argentina
Fil: Larzabal, Mariano. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Song, Huan. Chinese Academy of Sciences. South China Botanical Garden. Guangdong Provincial Key Laboratory of Applied Botany. Key Laboratory of South China Agricultural Plant Molecular Analysis and Genetic Improvement. Plant Gene Engineering Center; República Popular China
Fil: Cheng, Tianfang. Chinese Academy of Sciences. South China Botanical Garden. Guangdong Provincial Key Laboratory of Applied Botany. Key Laboratory of South China Agricultural Plant Molecular Analysis and Genetic Improvement. Plant Gene Engineering Center; República Popular China
Fil: Wang, Yufang. Chinese Academy of Sciences. South China Botanical Garden. Guangdong Provincial Key Laboratory of Applied Botany. Key Laboratory of South China Agricultural Plant Molecular Analysis and Genetic Improvement. Plant Gene Engineering Center; República Popular China
Fil: Smith, Libia Yael. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular (IABIMO); Argentina
Fil: Smith, Libia Yael. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Cataldi, Angel Adrian. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular (IABIMO); Argentina
Fil: Cataldi, Angel Adrian. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Ow, David W. Chinese Academy of Sciences. South China Botanical Garden. Guangdong Provincial Key Laboratory of Applied Botany. Key Laboratory of South China Agricultural Plant Molecular Analysis and Genetic Improvement. Plant Gene Engineering Center; República Popular China - Fuente
- Theoretical and Applied Genetics 136 (10) : 214 (Octubre 2023)
- Materia
-
Escherichia coli
Antígenos
Lechugas
Vacuna
Ratón
Antigens
Lettuces
Vaccines
Mice
Transgenes - Nivel de accesibilidad
- acceso restringido
- Condiciones de uso
- http://creativecommons.org/licenses/by-nc-sa/4.0/
- Repositorio
- Institución
- Instituto Nacional de Tecnología Agropecuaria
- OAI Identificador
- oai:localhost:20.500.12123/15662
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Enterohemorrhagic Escherichia coli O157:H7 antigens produced in transgenic lettuce effective as an oral vaccine in miceSu, Yun‑LinLarzabal, MarianoSong, HuanCheng, TianfangWang, YufangSmith, Libia YaelCataldi, Angel AdrianOw, David W.Escherichia coliAntígenosLechugasVacunaRatónAntigensLettucesVaccinesMiceTransgenesEnterohemorrhagic Escherichia coli (EHEC) O157:H7 is a food-borne pathogen where ruminant farm animals, mainly bovine, serve as reservoirs. Bovine vaccination has been used to prevent disease outbreaks, and the current method relies on vaccines subcutaneously injected three times per year. Since EHEC O157:H7 colonizes mucosal surfaces, an oral vaccine that produces an IgA response could be more convenient. Here, we report on oral vaccination against EHEC O157:H7 in mice orally gavaged with transgenic lettuce that produces EHEC O157:H7 antigens EspB and γ-intimin C280. Younger leaves accumulated a higher concentration of antigens; and in unexpanded leaves of 30-day-old T2 plants, EspB and γ-intimin C280 were up to 32 and 51 μg/g fresh weight, respectively. Mice orally gavaged with lettuce powders containing < 3 µg antigens for 6 days showed a mucosal immune response with reduced colonization of EHEC O157:H7. This suggests that the transgenic lettuce has potential to be used for bovine vaccination. To promote the biosafety of crop plants producing medically relevant proteins, recombination sites were built into our transgenic lines that would permit optional marker removal by Cre-lox recombination, as well as transgene deletion in pollen by CinH-RS2 recombination. The ability to upgrade the transgenic lettuce by stacking additional antigen genes or replacing older genes with newer versions would also be possible through the combined use of Bxb-att and Cre-lox recombination systems.Instituto de BiotecnologíaFil: Su, Yun‑Lin. Chinese Academy of Sciences. South China Botanical Garden. Guangdong Provincial Key Laboratory of Applied Botany. Key Laboratory of South China Agricultural Plant Molecular Analysis and Genetic Improvement. Plant Gene Engineering Center; República Popular ChinaFil: Larzabal, Mariano. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular (IABIMO); ArgentinaFil: Larzabal, Mariano. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Song, Huan. Chinese Academy of Sciences. South China Botanical Garden. Guangdong Provincial Key Laboratory of Applied Botany. Key Laboratory of South China Agricultural Plant Molecular Analysis and Genetic Improvement. Plant Gene Engineering Center; República Popular ChinaFil: Cheng, Tianfang. Chinese Academy of Sciences. South China Botanical Garden. Guangdong Provincial Key Laboratory of Applied Botany. Key Laboratory of South China Agricultural Plant Molecular Analysis and Genetic Improvement. Plant Gene Engineering Center; República Popular ChinaFil: Wang, Yufang. Chinese Academy of Sciences. South China Botanical Garden. Guangdong Provincial Key Laboratory of Applied Botany. Key Laboratory of South China Agricultural Plant Molecular Analysis and Genetic Improvement. Plant Gene Engineering Center; República Popular ChinaFil: Smith, Libia Yael. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular (IABIMO); ArgentinaFil: Smith, Libia Yael. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Cataldi, Angel Adrian. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular (IABIMO); ArgentinaFil: Cataldi, Angel Adrian. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Ow, David W. Chinese Academy of Sciences. South China Botanical Garden. Guangdong Provincial Key Laboratory of Applied Botany. Key Laboratory of South China Agricultural Plant Molecular Analysis and Genetic Improvement. Plant Gene Engineering Center; República Popular ChinaSpringer2023-10-24T15:00:03Z2023-10-24T15:00:03Z2023-10info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://hdl.handle.net/20.500.12123/15662https://link.springer.com/article/10.1007/s00122-023-04460-51432-2242https://doi.org/10.1007/s00122-023-04460-5Theoretical and Applied Genetics 136 (10) : 214 (Octubre 2023)reponame:INTA Digital (INTA)instname:Instituto Nacional de Tecnología Agropecuariaenginfo:eu-repo/semantics/restrictedAccesshttp://creativecommons.org/licenses/by-nc-sa/4.0/Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)2025-09-29T13:46:10Zoai:localhost:20.500.12123/15662instacron:INTAInstitucionalhttp://repositorio.inta.gob.ar/Organismo científico-tecnológicoNo correspondehttp://repositorio.inta.gob.ar/oai/requesttripaldi.nicolas@inta.gob.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:l2025-09-29 13:46:11.04INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuariafalse |
dc.title.none.fl_str_mv |
Enterohemorrhagic Escherichia coli O157:H7 antigens produced in transgenic lettuce effective as an oral vaccine in mice |
title |
Enterohemorrhagic Escherichia coli O157:H7 antigens produced in transgenic lettuce effective as an oral vaccine in mice |
spellingShingle |
Enterohemorrhagic Escherichia coli O157:H7 antigens produced in transgenic lettuce effective as an oral vaccine in mice Su, Yun‑Lin Escherichia coli Antígenos Lechugas Vacuna Ratón Antigens Lettuces Vaccines Mice Transgenes |
title_short |
Enterohemorrhagic Escherichia coli O157:H7 antigens produced in transgenic lettuce effective as an oral vaccine in mice |
title_full |
Enterohemorrhagic Escherichia coli O157:H7 antigens produced in transgenic lettuce effective as an oral vaccine in mice |
title_fullStr |
Enterohemorrhagic Escherichia coli O157:H7 antigens produced in transgenic lettuce effective as an oral vaccine in mice |
title_full_unstemmed |
Enterohemorrhagic Escherichia coli O157:H7 antigens produced in transgenic lettuce effective as an oral vaccine in mice |
title_sort |
Enterohemorrhagic Escherichia coli O157:H7 antigens produced in transgenic lettuce effective as an oral vaccine in mice |
dc.creator.none.fl_str_mv |
Su, Yun‑Lin Larzabal, Mariano Song, Huan Cheng, Tianfang Wang, Yufang Smith, Libia Yael Cataldi, Angel Adrian Ow, David W. |
author |
Su, Yun‑Lin |
author_facet |
Su, Yun‑Lin Larzabal, Mariano Song, Huan Cheng, Tianfang Wang, Yufang Smith, Libia Yael Cataldi, Angel Adrian Ow, David W. |
author_role |
author |
author2 |
Larzabal, Mariano Song, Huan Cheng, Tianfang Wang, Yufang Smith, Libia Yael Cataldi, Angel Adrian Ow, David W. |
author2_role |
author author author author author author author |
dc.subject.none.fl_str_mv |
Escherichia coli Antígenos Lechugas Vacuna Ratón Antigens Lettuces Vaccines Mice Transgenes |
topic |
Escherichia coli Antígenos Lechugas Vacuna Ratón Antigens Lettuces Vaccines Mice Transgenes |
dc.description.none.fl_txt_mv |
Enterohemorrhagic Escherichia coli (EHEC) O157:H7 is a food-borne pathogen where ruminant farm animals, mainly bovine, serve as reservoirs. Bovine vaccination has been used to prevent disease outbreaks, and the current method relies on vaccines subcutaneously injected three times per year. Since EHEC O157:H7 colonizes mucosal surfaces, an oral vaccine that produces an IgA response could be more convenient. Here, we report on oral vaccination against EHEC O157:H7 in mice orally gavaged with transgenic lettuce that produces EHEC O157:H7 antigens EspB and γ-intimin C280. Younger leaves accumulated a higher concentration of antigens; and in unexpanded leaves of 30-day-old T2 plants, EspB and γ-intimin C280 were up to 32 and 51 μg/g fresh weight, respectively. Mice orally gavaged with lettuce powders containing < 3 µg antigens for 6 days showed a mucosal immune response with reduced colonization of EHEC O157:H7. This suggests that the transgenic lettuce has potential to be used for bovine vaccination. To promote the biosafety of crop plants producing medically relevant proteins, recombination sites were built into our transgenic lines that would permit optional marker removal by Cre-lox recombination, as well as transgene deletion in pollen by CinH-RS2 recombination. The ability to upgrade the transgenic lettuce by stacking additional antigen genes or replacing older genes with newer versions would also be possible through the combined use of Bxb-att and Cre-lox recombination systems. Instituto de Biotecnología Fil: Su, Yun‑Lin. Chinese Academy of Sciences. South China Botanical Garden. Guangdong Provincial Key Laboratory of Applied Botany. Key Laboratory of South China Agricultural Plant Molecular Analysis and Genetic Improvement. Plant Gene Engineering Center; República Popular China Fil: Larzabal, Mariano. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular (IABIMO); Argentina Fil: Larzabal, Mariano. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Song, Huan. Chinese Academy of Sciences. South China Botanical Garden. Guangdong Provincial Key Laboratory of Applied Botany. Key Laboratory of South China Agricultural Plant Molecular Analysis and Genetic Improvement. Plant Gene Engineering Center; República Popular China Fil: Cheng, Tianfang. Chinese Academy of Sciences. South China Botanical Garden. Guangdong Provincial Key Laboratory of Applied Botany. Key Laboratory of South China Agricultural Plant Molecular Analysis and Genetic Improvement. Plant Gene Engineering Center; República Popular China Fil: Wang, Yufang. Chinese Academy of Sciences. South China Botanical Garden. Guangdong Provincial Key Laboratory of Applied Botany. Key Laboratory of South China Agricultural Plant Molecular Analysis and Genetic Improvement. Plant Gene Engineering Center; República Popular China Fil: Smith, Libia Yael. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular (IABIMO); Argentina Fil: Smith, Libia Yael. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Cataldi, Angel Adrian. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular (IABIMO); Argentina Fil: Cataldi, Angel Adrian. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Ow, David W. Chinese Academy of Sciences. South China Botanical Garden. Guangdong Provincial Key Laboratory of Applied Botany. Key Laboratory of South China Agricultural Plant Molecular Analysis and Genetic Improvement. Plant Gene Engineering Center; República Popular China |
description |
Enterohemorrhagic Escherichia coli (EHEC) O157:H7 is a food-borne pathogen where ruminant farm animals, mainly bovine, serve as reservoirs. Bovine vaccination has been used to prevent disease outbreaks, and the current method relies on vaccines subcutaneously injected three times per year. Since EHEC O157:H7 colonizes mucosal surfaces, an oral vaccine that produces an IgA response could be more convenient. Here, we report on oral vaccination against EHEC O157:H7 in mice orally gavaged with transgenic lettuce that produces EHEC O157:H7 antigens EspB and γ-intimin C280. Younger leaves accumulated a higher concentration of antigens; and in unexpanded leaves of 30-day-old T2 plants, EspB and γ-intimin C280 were up to 32 and 51 μg/g fresh weight, respectively. Mice orally gavaged with lettuce powders containing < 3 µg antigens for 6 days showed a mucosal immune response with reduced colonization of EHEC O157:H7. This suggests that the transgenic lettuce has potential to be used for bovine vaccination. To promote the biosafety of crop plants producing medically relevant proteins, recombination sites were built into our transgenic lines that would permit optional marker removal by Cre-lox recombination, as well as transgene deletion in pollen by CinH-RS2 recombination. The ability to upgrade the transgenic lettuce by stacking additional antigen genes or replacing older genes with newer versions would also be possible through the combined use of Bxb-att and Cre-lox recombination systems. |
publishDate |
2023 |
dc.date.none.fl_str_mv |
2023-10-24T15:00:03Z 2023-10-24T15:00:03Z 2023-10 |
dc.type.none.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://purl.org/coar/resource_type/c_6501 info:ar-repo/semantics/articulo |
format |
article |
status_str |
publishedVersion |
dc.identifier.none.fl_str_mv |
http://hdl.handle.net/20.500.12123/15662 https://link.springer.com/article/10.1007/s00122-023-04460-5 1432-2242 https://doi.org/10.1007/s00122-023-04460-5 |
url |
http://hdl.handle.net/20.500.12123/15662 https://link.springer.com/article/10.1007/s00122-023-04460-5 https://doi.org/10.1007/s00122-023-04460-5 |
identifier_str_mv |
1432-2242 |
dc.language.none.fl_str_mv |
eng |
language |
eng |
dc.rights.none.fl_str_mv |
info:eu-repo/semantics/restrictedAccess http://creativecommons.org/licenses/by-nc-sa/4.0/ Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) |
eu_rights_str_mv |
restrictedAccess |
rights_invalid_str_mv |
http://creativecommons.org/licenses/by-nc-sa/4.0/ Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Springer |
publisher.none.fl_str_mv |
Springer |
dc.source.none.fl_str_mv |
Theoretical and Applied Genetics 136 (10) : 214 (Octubre 2023) reponame:INTA Digital (INTA) instname:Instituto Nacional de Tecnología Agropecuaria |
reponame_str |
INTA Digital (INTA) |
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Instituto Nacional de Tecnología Agropecuaria |
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INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuaria |
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tripaldi.nicolas@inta.gob.ar |
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