Immunogenicity of hypothetical highly conserved proteins as novel antigens in Anaplasma marginale

Autores
Nuñez, Pablo Alfredo; Moretta, Rosalia Ester; Ruybal, Paula; Wilkowsky, Silvina Elizabeth; Farber, Marisa Diana
Año de publicación
2014
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Anaplasma marginale is a tick-transmitted Gram-negative intraerythrocytic bacterium and the etiological agent of bovine Anaplasmosis. Even though considerable research efforts have been undertaken, Anaplasmosis vaccine development remains a challenging field. Outer-membrane-specific antigens responsible for the ability of more complex immunogens could have a significant role in the protective response. Thus, the identification of outer-membrane antigens represents a major goal in the development of bacterial vaccines. Considering that 40 % of the annotated proteins in A. marginale remain as hypothetical, we selected three candidate antigens, AM1108, AM127, and AM216 based on experimental evidence, in silico structure prediction of β-barrel outer membrane, and orthology clustering. Sequence alignment and analysis demonstrated a high degree of conservation for the three proteins between the isolates from Argentina compared to the American strains. We confirmed the transcription of the three genes in the intraerythrocytic stage. AM1108 and AM216 recombinant proteins elicited specific T-cell response proliferation and a significant rise in TNF-α and IFN-γ transcript levels, respectively. Only AM1108 was able to be recognized by specific antibodies from infected bovines. This study allowed the identification of new candidate components of the outer-membrane fraction of A. marginale. Further studies will be required to analyze their potential as effective antigens for being included in rational vaccine strategies.
Instituto de Biotecnología
Fil: Nuñez, Pablo Alfredo. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina
Fil: Moretta, Rosalia Ester. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina
Fil: Ruybal, Paula. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina
Fil: Wilkowsky, Silvina Elizabeth. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina
Fil: Farber, Marisa Diana. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina
Fuente
Current Microbiology 68 (3) : 269–277 (March 2014)
Materia
Anaplasma Marginale
Inmunogenética
Antígenos
Antigenos Bacterianos
Vacuna
Immunogenetics
Antigens
Bacterial Antigens
Vaccines
Nivel de accesibilidad
acceso restringido
Condiciones de uso
Repositorio
INTA Digital (INTA)
Institución
Instituto Nacional de Tecnología Agropecuaria
OAI Identificador
oai:localhost:20.500.12123/4291
Acceder
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oai_identifier_str oai:localhost:20.500.12123/4291
network_acronym_str INTADig
repository_id_str l
network_name_str INTA Digital (INTA)
spelling Immunogenicity of hypothetical highly conserved proteins as novel antigens in Anaplasma marginaleNuñez, Pablo AlfredoMoretta, Rosalia EsterRuybal, PaulaWilkowsky, Silvina ElizabethFarber, Marisa DianaAnaplasma MarginaleInmunogenéticaAntígenosAntigenos BacterianosVacunaImmunogeneticsAntigensBacterial AntigensVaccinesAnaplasma marginale is a tick-transmitted Gram-negative intraerythrocytic bacterium and the etiological agent of bovine Anaplasmosis. Even though considerable research efforts have been undertaken, Anaplasmosis vaccine development remains a challenging field. Outer-membrane-specific antigens responsible for the ability of more complex immunogens could have a significant role in the protective response. Thus, the identification of outer-membrane antigens represents a major goal in the development of bacterial vaccines. Considering that 40 % of the annotated proteins in A. marginale remain as hypothetical, we selected three candidate antigens, AM1108, AM127, and AM216 based on experimental evidence, in silico structure prediction of β-barrel outer membrane, and orthology clustering. Sequence alignment and analysis demonstrated a high degree of conservation for the three proteins between the isolates from Argentina compared to the American strains. We confirmed the transcription of the three genes in the intraerythrocytic stage. AM1108 and AM216 recombinant proteins elicited specific T-cell response proliferation and a significant rise in TNF-α and IFN-γ transcript levels, respectively. Only AM1108 was able to be recognized by specific antibodies from infected bovines. This study allowed the identification of new candidate components of the outer-membrane fraction of A. marginale. Further studies will be required to analyze their potential as effective antigens for being included in rational vaccine strategies.Instituto de BiotecnologíaFil: Nuñez, Pablo Alfredo. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; ArgentinaFil: Moretta, Rosalia Ester. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; ArgentinaFil: Ruybal, Paula. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; ArgentinaFil: Wilkowsky, Silvina Elizabeth. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; ArgentinaFil: Farber, Marisa Diana. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; ArgentinaSpringer2019-01-18T12:04:03Z2019-01-18T12:04:03Z2014-03info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttps://link.springer.com/article/10.1007/s00284-013-0475-6http://hdl.handle.net/20.500.12123/42910343-86511432-0991https://doi.org/10.1007/s00284-013-0475-6Current Microbiology 68 (3) : 269–277 (March 2014)reponame:INTA Digital (INTA)instname:Instituto Nacional de Tecnología Agropecuariaenginfo:eu-repo/semantics/restrictedAccess2025-09-29T13:44:33Zoai:localhost:20.500.12123/4291instacron:INTAInstitucionalhttp://repositorio.inta.gob.ar/Organismo científico-tecnológicoNo correspondehttp://repositorio.inta.gob.ar/oai/requesttripaldi.nicolas@inta.gob.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:l2025-09-29 13:44:33.502INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuariafalse
dc.title.none.fl_str_mv Immunogenicity of hypothetical highly conserved proteins as novel antigens in Anaplasma marginale
title Immunogenicity of hypothetical highly conserved proteins as novel antigens in Anaplasma marginale
spellingShingle Immunogenicity of hypothetical highly conserved proteins as novel antigens in Anaplasma marginale
Nuñez, Pablo Alfredo
Anaplasma Marginale
Inmunogenética
Antígenos
Antigenos Bacterianos
Vacuna
Immunogenetics
Antigens
Bacterial Antigens
Vaccines
title_short Immunogenicity of hypothetical highly conserved proteins as novel antigens in Anaplasma marginale
title_full Immunogenicity of hypothetical highly conserved proteins as novel antigens in Anaplasma marginale
title_fullStr Immunogenicity of hypothetical highly conserved proteins as novel antigens in Anaplasma marginale
title_full_unstemmed Immunogenicity of hypothetical highly conserved proteins as novel antigens in Anaplasma marginale
title_sort Immunogenicity of hypothetical highly conserved proteins as novel antigens in Anaplasma marginale
dc.creator.none.fl_str_mv Nuñez, Pablo Alfredo
Moretta, Rosalia Ester
Ruybal, Paula
Wilkowsky, Silvina Elizabeth
Farber, Marisa Diana
author Nuñez, Pablo Alfredo
author_facet Nuñez, Pablo Alfredo
Moretta, Rosalia Ester
Ruybal, Paula
Wilkowsky, Silvina Elizabeth
Farber, Marisa Diana
author_role author
author2 Moretta, Rosalia Ester
Ruybal, Paula
Wilkowsky, Silvina Elizabeth
Farber, Marisa Diana
author2_role author
author
author
author
dc.subject.none.fl_str_mv Anaplasma Marginale
Inmunogenética
Antígenos
Antigenos Bacterianos
Vacuna
Immunogenetics
Antigens
Bacterial Antigens
Vaccines
topic Anaplasma Marginale
Inmunogenética
Antígenos
Antigenos Bacterianos
Vacuna
Immunogenetics
Antigens
Bacterial Antigens
Vaccines
dc.description.none.fl_txt_mv Anaplasma marginale is a tick-transmitted Gram-negative intraerythrocytic bacterium and the etiological agent of bovine Anaplasmosis. Even though considerable research efforts have been undertaken, Anaplasmosis vaccine development remains a challenging field. Outer-membrane-specific antigens responsible for the ability of more complex immunogens could have a significant role in the protective response. Thus, the identification of outer-membrane antigens represents a major goal in the development of bacterial vaccines. Considering that 40 % of the annotated proteins in A. marginale remain as hypothetical, we selected three candidate antigens, AM1108, AM127, and AM216 based on experimental evidence, in silico structure prediction of β-barrel outer membrane, and orthology clustering. Sequence alignment and analysis demonstrated a high degree of conservation for the three proteins between the isolates from Argentina compared to the American strains. We confirmed the transcription of the three genes in the intraerythrocytic stage. AM1108 and AM216 recombinant proteins elicited specific T-cell response proliferation and a significant rise in TNF-α and IFN-γ transcript levels, respectively. Only AM1108 was able to be recognized by specific antibodies from infected bovines. This study allowed the identification of new candidate components of the outer-membrane fraction of A. marginale. Further studies will be required to analyze their potential as effective antigens for being included in rational vaccine strategies.
Instituto de Biotecnología
Fil: Nuñez, Pablo Alfredo. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina
Fil: Moretta, Rosalia Ester. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina
Fil: Ruybal, Paula. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina
Fil: Wilkowsky, Silvina Elizabeth. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina
Fil: Farber, Marisa Diana. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina
description Anaplasma marginale is a tick-transmitted Gram-negative intraerythrocytic bacterium and the etiological agent of bovine Anaplasmosis. Even though considerable research efforts have been undertaken, Anaplasmosis vaccine development remains a challenging field. Outer-membrane-specific antigens responsible for the ability of more complex immunogens could have a significant role in the protective response. Thus, the identification of outer-membrane antigens represents a major goal in the development of bacterial vaccines. Considering that 40 % of the annotated proteins in A. marginale remain as hypothetical, we selected three candidate antigens, AM1108, AM127, and AM216 based on experimental evidence, in silico structure prediction of β-barrel outer membrane, and orthology clustering. Sequence alignment and analysis demonstrated a high degree of conservation for the three proteins between the isolates from Argentina compared to the American strains. We confirmed the transcription of the three genes in the intraerythrocytic stage. AM1108 and AM216 recombinant proteins elicited specific T-cell response proliferation and a significant rise in TNF-α and IFN-γ transcript levels, respectively. Only AM1108 was able to be recognized by specific antibodies from infected bovines. This study allowed the identification of new candidate components of the outer-membrane fraction of A. marginale. Further studies will be required to analyze their potential as effective antigens for being included in rational vaccine strategies.
publishDate 2014
dc.date.none.fl_str_mv 2014-03
2019-01-18T12:04:03Z
2019-01-18T12:04:03Z
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv https://link.springer.com/article/10.1007/s00284-013-0475-6
http://hdl.handle.net/20.500.12123/4291
0343-8651
1432-0991
https://doi.org/10.1007/s00284-013-0475-6
url https://link.springer.com/article/10.1007/s00284-013-0475-6
http://hdl.handle.net/20.500.12123/4291
https://doi.org/10.1007/s00284-013-0475-6
identifier_str_mv 0343-8651
1432-0991
dc.language.none.fl_str_mv eng
language eng
dc.rights.none.fl_str_mv info:eu-repo/semantics/restrictedAccess
eu_rights_str_mv restrictedAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Springer
publisher.none.fl_str_mv Springer
dc.source.none.fl_str_mv Current Microbiology 68 (3) : 269–277 (March 2014)
reponame:INTA Digital (INTA)
instname:Instituto Nacional de Tecnología Agropecuaria
reponame_str INTA Digital (INTA)
collection INTA Digital (INTA)
instname_str Instituto Nacional de Tecnología Agropecuaria
repository.name.fl_str_mv INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuaria
repository.mail.fl_str_mv tripaldi.nicolas@inta.gob.ar
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score 12.558318

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