Immunogenicity of hypothetical highly conserved proteins as novel antigens in Anaplasma marginale
- Autores
- Nuñez, Pablo Alfredo; Moretta, Rosalia Ester; Ruybal, Paula; Wilkowsky, Silvina Elizabeth; Farber, Marisa Diana
- Año de publicación
- 2014
- Idioma
- inglés
- Tipo de recurso
- artículo
- Estado
- versión publicada
- Descripción
- Anaplasma marginale is a tick-transmitted Gram-negative intraerythrocytic bacterium and the etiological agent of bovine Anaplasmosis. Even though considerable research efforts have been undertaken, Anaplasmosis vaccine development remains a challenging field. Outer-membrane-specific antigens responsible for the ability of more complex immunogens could have a significant role in the protective response. Thus, the identification of outer-membrane antigens represents a major goal in the development of bacterial vaccines. Considering that 40 % of the annotated proteins in A. marginale remain as hypothetical, we selected three candidate antigens, AM1108, AM127, and AM216 based on experimental evidence, in silico structure prediction of β-barrel outer membrane, and orthology clustering. Sequence alignment and analysis demonstrated a high degree of conservation for the three proteins between the isolates from Argentina compared to the American strains. We confirmed the transcription of the three genes in the intraerythrocytic stage. AM1108 and AM216 recombinant proteins elicited specific T-cell response proliferation and a significant rise in TNF-α and IFN-γ transcript levels, respectively. Only AM1108 was able to be recognized by specific antibodies from infected bovines. This study allowed the identification of new candidate components of the outer-membrane fraction of A. marginale. Further studies will be required to analyze their potential as effective antigens for being included in rational vaccine strategies.
Instituto de Biotecnología
Fil: Nuñez, Pablo Alfredo. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina
Fil: Moretta, Rosalia Ester. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina
Fil: Ruybal, Paula. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina
Fil: Wilkowsky, Silvina Elizabeth. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina
Fil: Farber, Marisa Diana. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina - Fuente
- Current Microbiology 68 (3) : 269–277 (March 2014)
- Materia
-
Anaplasma Marginale
Inmunogenética
Antígenos
Antigenos Bacterianos
Vacuna
Immunogenetics
Antigens
Bacterial Antigens
Vaccines - Nivel de accesibilidad
- acceso restringido
- Condiciones de uso
- Repositorio
- Institución
- Instituto Nacional de Tecnología Agropecuaria
- OAI Identificador
- oai:localhost:20.500.12123/4291
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Immunogenicity of hypothetical highly conserved proteins as novel antigens in Anaplasma marginaleNuñez, Pablo AlfredoMoretta, Rosalia EsterRuybal, PaulaWilkowsky, Silvina ElizabethFarber, Marisa DianaAnaplasma MarginaleInmunogenéticaAntígenosAntigenos BacterianosVacunaImmunogeneticsAntigensBacterial AntigensVaccinesAnaplasma marginale is a tick-transmitted Gram-negative intraerythrocytic bacterium and the etiological agent of bovine Anaplasmosis. Even though considerable research efforts have been undertaken, Anaplasmosis vaccine development remains a challenging field. Outer-membrane-specific antigens responsible for the ability of more complex immunogens could have a significant role in the protective response. Thus, the identification of outer-membrane antigens represents a major goal in the development of bacterial vaccines. Considering that 40 % of the annotated proteins in A. marginale remain as hypothetical, we selected three candidate antigens, AM1108, AM127, and AM216 based on experimental evidence, in silico structure prediction of β-barrel outer membrane, and orthology clustering. Sequence alignment and analysis demonstrated a high degree of conservation for the three proteins between the isolates from Argentina compared to the American strains. We confirmed the transcription of the three genes in the intraerythrocytic stage. AM1108 and AM216 recombinant proteins elicited specific T-cell response proliferation and a significant rise in TNF-α and IFN-γ transcript levels, respectively. Only AM1108 was able to be recognized by specific antibodies from infected bovines. This study allowed the identification of new candidate components of the outer-membrane fraction of A. marginale. Further studies will be required to analyze their potential as effective antigens for being included in rational vaccine strategies.Instituto de BiotecnologíaFil: Nuñez, Pablo Alfredo. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; ArgentinaFil: Moretta, Rosalia Ester. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; ArgentinaFil: Ruybal, Paula. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; ArgentinaFil: Wilkowsky, Silvina Elizabeth. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; ArgentinaFil: Farber, Marisa Diana. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; ArgentinaSpringer2019-01-18T12:04:03Z2019-01-18T12:04:03Z2014-03info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttps://link.springer.com/article/10.1007/s00284-013-0475-6http://hdl.handle.net/20.500.12123/42910343-86511432-0991https://doi.org/10.1007/s00284-013-0475-6Current Microbiology 68 (3) : 269–277 (March 2014)reponame:INTA Digital (INTA)instname:Instituto Nacional de Tecnología Agropecuariaenginfo:eu-repo/semantics/restrictedAccess2025-09-04T09:47:46Zoai:localhost:20.500.12123/4291instacron:INTAInstitucionalhttp://repositorio.inta.gob.ar/Organismo científico-tecnológicoNo correspondehttp://repositorio.inta.gob.ar/oai/requesttripaldi.nicolas@inta.gob.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:l2025-09-04 09:47:46.907INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuariafalse |
dc.title.none.fl_str_mv |
Immunogenicity of hypothetical highly conserved proteins as novel antigens in Anaplasma marginale |
title |
Immunogenicity of hypothetical highly conserved proteins as novel antigens in Anaplasma marginale |
spellingShingle |
Immunogenicity of hypothetical highly conserved proteins as novel antigens in Anaplasma marginale Nuñez, Pablo Alfredo Anaplasma Marginale Inmunogenética Antígenos Antigenos Bacterianos Vacuna Immunogenetics Antigens Bacterial Antigens Vaccines |
title_short |
Immunogenicity of hypothetical highly conserved proteins as novel antigens in Anaplasma marginale |
title_full |
Immunogenicity of hypothetical highly conserved proteins as novel antigens in Anaplasma marginale |
title_fullStr |
Immunogenicity of hypothetical highly conserved proteins as novel antigens in Anaplasma marginale |
title_full_unstemmed |
Immunogenicity of hypothetical highly conserved proteins as novel antigens in Anaplasma marginale |
title_sort |
Immunogenicity of hypothetical highly conserved proteins as novel antigens in Anaplasma marginale |
dc.creator.none.fl_str_mv |
Nuñez, Pablo Alfredo Moretta, Rosalia Ester Ruybal, Paula Wilkowsky, Silvina Elizabeth Farber, Marisa Diana |
author |
Nuñez, Pablo Alfredo |
author_facet |
Nuñez, Pablo Alfredo Moretta, Rosalia Ester Ruybal, Paula Wilkowsky, Silvina Elizabeth Farber, Marisa Diana |
author_role |
author |
author2 |
Moretta, Rosalia Ester Ruybal, Paula Wilkowsky, Silvina Elizabeth Farber, Marisa Diana |
author2_role |
author author author author |
dc.subject.none.fl_str_mv |
Anaplasma Marginale Inmunogenética Antígenos Antigenos Bacterianos Vacuna Immunogenetics Antigens Bacterial Antigens Vaccines |
topic |
Anaplasma Marginale Inmunogenética Antígenos Antigenos Bacterianos Vacuna Immunogenetics Antigens Bacterial Antigens Vaccines |
dc.description.none.fl_txt_mv |
Anaplasma marginale is a tick-transmitted Gram-negative intraerythrocytic bacterium and the etiological agent of bovine Anaplasmosis. Even though considerable research efforts have been undertaken, Anaplasmosis vaccine development remains a challenging field. Outer-membrane-specific antigens responsible for the ability of more complex immunogens could have a significant role in the protective response. Thus, the identification of outer-membrane antigens represents a major goal in the development of bacterial vaccines. Considering that 40 % of the annotated proteins in A. marginale remain as hypothetical, we selected three candidate antigens, AM1108, AM127, and AM216 based on experimental evidence, in silico structure prediction of β-barrel outer membrane, and orthology clustering. Sequence alignment and analysis demonstrated a high degree of conservation for the three proteins between the isolates from Argentina compared to the American strains. We confirmed the transcription of the three genes in the intraerythrocytic stage. AM1108 and AM216 recombinant proteins elicited specific T-cell response proliferation and a significant rise in TNF-α and IFN-γ transcript levels, respectively. Only AM1108 was able to be recognized by specific antibodies from infected bovines. This study allowed the identification of new candidate components of the outer-membrane fraction of A. marginale. Further studies will be required to analyze their potential as effective antigens for being included in rational vaccine strategies. Instituto de Biotecnología Fil: Nuñez, Pablo Alfredo. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina Fil: Moretta, Rosalia Ester. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina Fil: Ruybal, Paula. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina Fil: Wilkowsky, Silvina Elizabeth. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina Fil: Farber, Marisa Diana. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina |
description |
Anaplasma marginale is a tick-transmitted Gram-negative intraerythrocytic bacterium and the etiological agent of bovine Anaplasmosis. Even though considerable research efforts have been undertaken, Anaplasmosis vaccine development remains a challenging field. Outer-membrane-specific antigens responsible for the ability of more complex immunogens could have a significant role in the protective response. Thus, the identification of outer-membrane antigens represents a major goal in the development of bacterial vaccines. Considering that 40 % of the annotated proteins in A. marginale remain as hypothetical, we selected three candidate antigens, AM1108, AM127, and AM216 based on experimental evidence, in silico structure prediction of β-barrel outer membrane, and orthology clustering. Sequence alignment and analysis demonstrated a high degree of conservation for the three proteins between the isolates from Argentina compared to the American strains. We confirmed the transcription of the three genes in the intraerythrocytic stage. AM1108 and AM216 recombinant proteins elicited specific T-cell response proliferation and a significant rise in TNF-α and IFN-γ transcript levels, respectively. Only AM1108 was able to be recognized by specific antibodies from infected bovines. This study allowed the identification of new candidate components of the outer-membrane fraction of A. marginale. Further studies will be required to analyze their potential as effective antigens for being included in rational vaccine strategies. |
publishDate |
2014 |
dc.date.none.fl_str_mv |
2014-03 2019-01-18T12:04:03Z 2019-01-18T12:04:03Z |
dc.type.none.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://purl.org/coar/resource_type/c_6501 info:ar-repo/semantics/articulo |
format |
article |
status_str |
publishedVersion |
dc.identifier.none.fl_str_mv |
https://link.springer.com/article/10.1007/s00284-013-0475-6 http://hdl.handle.net/20.500.12123/4291 0343-8651 1432-0991 https://doi.org/10.1007/s00284-013-0475-6 |
url |
https://link.springer.com/article/10.1007/s00284-013-0475-6 http://hdl.handle.net/20.500.12123/4291 https://doi.org/10.1007/s00284-013-0475-6 |
identifier_str_mv |
0343-8651 1432-0991 |
dc.language.none.fl_str_mv |
eng |
language |
eng |
dc.rights.none.fl_str_mv |
info:eu-repo/semantics/restrictedAccess |
eu_rights_str_mv |
restrictedAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Springer |
publisher.none.fl_str_mv |
Springer |
dc.source.none.fl_str_mv |
Current Microbiology 68 (3) : 269–277 (March 2014) reponame:INTA Digital (INTA) instname:Instituto Nacional de Tecnología Agropecuaria |
reponame_str |
INTA Digital (INTA) |
collection |
INTA Digital (INTA) |
instname_str |
Instituto Nacional de Tecnología Agropecuaria |
repository.name.fl_str_mv |
INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuaria |
repository.mail.fl_str_mv |
tripaldi.nicolas@inta.gob.ar |
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12.623145 |