Evidence of Recombinant Isolates of Potato Virus Y (PVY) in Argentina

Autores
Colavita, Monica; Lancia, Marcos; Feingold, Sergio Enrique; Massa, Gabriela Alejandra
Año de publicación
2017
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
In order to make a first approach in the identification of the genetic diversity of Potato virus Y (PVY) in Argentina, 46 PVY isolates from different potato growing regions of Argentina were characterized both, biological and serologically. Five of them (ST11, RCA5a, RCA6, RCA14b and SSF6) were selected for further genomic analyses. Four genomic fragments containing hot spot regions of recombination (HSR) reported previously were sequenced in each isolate and compared to PVYN (CS434575.1) and PVYO (U09509.1) reference genomes looking for genomic recombinations. Isolates with one, two or three recombination points were identified among these, including the two strains considered typical PVYN (RCA5b) and PVYO (SSF18) used as controls. This is the first report of the presence of recombined PVY in Argentina using a combination of biological, serological and molecular tools that shed light on the genetic diversity of PVY viruses in this country.
EEA Balcarce
Fil: Colavita, Mónica L. Universidad Nacional de Mar del Plata. Facultad de Ciencias Agrarias, Balcarce; Argentina
Fil: Lancia, Marcos. Universidad Nacional de Mar del Plata, Facultad de Ciencias Exactas y Naturales; Argentina. Universidad Nacional de La Plata. Instituto de Biotecnología y Biología Molecular, La Plata; Argentina
Fil: Feingold, Sergio. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina
Fil: Massa, Gabriela. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina. Universidad Nacional de Mar del Plata, Facultad de Ciencias Agrarias, Balcarce; Argentina
Fuente
American Journal of Potato Research 94 (3) : 275-282 (June 2017 )
Materia
Papa
Virus
Diversidad Genética
Recombinación
Argentina
Potatoes
Viruses
Genetic Diversity
Recombination
Potato Virus Y
Virus Y Patata
Nivel de accesibilidad
acceso restringido
Condiciones de uso
Repositorio
INTA Digital (INTA)
Institución
Instituto Nacional de Tecnología Agropecuaria
OAI Identificador
oai:localhost:20.500.12123/3163
Acceder
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oai_identifier_str oai:localhost:20.500.12123/3163
network_acronym_str INTADig
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network_name_str INTA Digital (INTA)
spelling Evidence of Recombinant Isolates of Potato Virus Y (PVY) in ArgentinaColavita, MonicaLancia, MarcosFeingold, Sergio EnriqueMassa, Gabriela AlejandraPapaVirusDiversidad GenéticaRecombinaciónArgentinaPotatoesVirusesGenetic DiversityRecombinationPotato Virus YVirus Y PatataIn order to make a first approach in the identification of the genetic diversity of Potato virus Y (PVY) in Argentina, 46 PVY isolates from different potato growing regions of Argentina were characterized both, biological and serologically. Five of them (ST11, RCA5a, RCA6, RCA14b and SSF6) were selected for further genomic analyses. Four genomic fragments containing hot spot regions of recombination (HSR) reported previously were sequenced in each isolate and compared to PVYN (CS434575.1) and PVYO (U09509.1) reference genomes looking for genomic recombinations. Isolates with one, two or three recombination points were identified among these, including the two strains considered typical PVYN (RCA5b) and PVYO (SSF18) used as controls. This is the first report of the presence of recombined PVY in Argentina using a combination of biological, serological and molecular tools that shed light on the genetic diversity of PVY viruses in this country.EEA BalcarceFil: Colavita, Mónica L. Universidad Nacional de Mar del Plata. Facultad de Ciencias Agrarias, Balcarce; ArgentinaFil: Lancia, Marcos. Universidad Nacional de Mar del Plata, Facultad de Ciencias Exactas y Naturales; Argentina. Universidad Nacional de La Plata. Instituto de Biotecnología y Biología Molecular, La Plata; ArgentinaFil: Feingold, Sergio. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; ArgentinaFil: Massa, Gabriela. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina. Universidad Nacional de Mar del Plata, Facultad de Ciencias Agrarias, Balcarce; ArgentinaSpringer Netherlands2018-08-23T13:27:49Z2018-08-23T13:27:49Z2017-06info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttps://link.springer.com/article/10.1007/s12230-016-9564-yhttp://hdl.handle.net/20.500.12123/31631099-209X1874-9380https://doi.org/10.1007/s12230-016-9564-yAmerican Journal of Potato Research 94 (3) : 275-282 (June 2017 )reponame:INTA Digital (INTA)instname:Instituto Nacional de Tecnología Agropecuariaenginfo:eu-repo/semantics/restrictedAccess2025-09-04T09:47:26Zoai:localhost:20.500.12123/3163instacron:INTAInstitucionalhttp://repositorio.inta.gob.ar/Organismo científico-tecnológicoNo correspondehttp://repositorio.inta.gob.ar/oai/requesttripaldi.nicolas@inta.gob.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:l2025-09-04 09:47:26.543INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuariafalse
dc.title.none.fl_str_mv Evidence of Recombinant Isolates of Potato Virus Y (PVY) in Argentina
title Evidence of Recombinant Isolates of Potato Virus Y (PVY) in Argentina
spellingShingle Evidence of Recombinant Isolates of Potato Virus Y (PVY) in Argentina
Colavita, Monica
Papa
Virus
Diversidad Genética
Recombinación
Argentina
Potatoes
Viruses
Genetic Diversity
Recombination
Potato Virus Y
Virus Y Patata
title_short Evidence of Recombinant Isolates of Potato Virus Y (PVY) in Argentina
title_full Evidence of Recombinant Isolates of Potato Virus Y (PVY) in Argentina
title_fullStr Evidence of Recombinant Isolates of Potato Virus Y (PVY) in Argentina
title_full_unstemmed Evidence of Recombinant Isolates of Potato Virus Y (PVY) in Argentina
title_sort Evidence of Recombinant Isolates of Potato Virus Y (PVY) in Argentina
dc.creator.none.fl_str_mv Colavita, Monica
Lancia, Marcos
Feingold, Sergio Enrique
Massa, Gabriela Alejandra
author Colavita, Monica
author_facet Colavita, Monica
Lancia, Marcos
Feingold, Sergio Enrique
Massa, Gabriela Alejandra
author_role author
author2 Lancia, Marcos
Feingold, Sergio Enrique
Massa, Gabriela Alejandra
author2_role author
author
author
dc.subject.none.fl_str_mv Papa
Virus
Diversidad Genética
Recombinación
Argentina
Potatoes
Viruses
Genetic Diversity
Recombination
Potato Virus Y
Virus Y Patata
topic Papa
Virus
Diversidad Genética
Recombinación
Argentina
Potatoes
Viruses
Genetic Diversity
Recombination
Potato Virus Y
Virus Y Patata
dc.description.none.fl_txt_mv In order to make a first approach in the identification of the genetic diversity of Potato virus Y (PVY) in Argentina, 46 PVY isolates from different potato growing regions of Argentina were characterized both, biological and serologically. Five of them (ST11, RCA5a, RCA6, RCA14b and SSF6) were selected for further genomic analyses. Four genomic fragments containing hot spot regions of recombination (HSR) reported previously were sequenced in each isolate and compared to PVYN (CS434575.1) and PVYO (U09509.1) reference genomes looking for genomic recombinations. Isolates with one, two or three recombination points were identified among these, including the two strains considered typical PVYN (RCA5b) and PVYO (SSF18) used as controls. This is the first report of the presence of recombined PVY in Argentina using a combination of biological, serological and molecular tools that shed light on the genetic diversity of PVY viruses in this country.
EEA Balcarce
Fil: Colavita, Mónica L. Universidad Nacional de Mar del Plata. Facultad de Ciencias Agrarias, Balcarce; Argentina
Fil: Lancia, Marcos. Universidad Nacional de Mar del Plata, Facultad de Ciencias Exactas y Naturales; Argentina. Universidad Nacional de La Plata. Instituto de Biotecnología y Biología Molecular, La Plata; Argentina
Fil: Feingold, Sergio. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina
Fil: Massa, Gabriela. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina. Universidad Nacional de Mar del Plata, Facultad de Ciencias Agrarias, Balcarce; Argentina
description In order to make a first approach in the identification of the genetic diversity of Potato virus Y (PVY) in Argentina, 46 PVY isolates from different potato growing regions of Argentina were characterized both, biological and serologically. Five of them (ST11, RCA5a, RCA6, RCA14b and SSF6) were selected for further genomic analyses. Four genomic fragments containing hot spot regions of recombination (HSR) reported previously were sequenced in each isolate and compared to PVYN (CS434575.1) and PVYO (U09509.1) reference genomes looking for genomic recombinations. Isolates with one, two or three recombination points were identified among these, including the two strains considered typical PVYN (RCA5b) and PVYO (SSF18) used as controls. This is the first report of the presence of recombined PVY in Argentina using a combination of biological, serological and molecular tools that shed light on the genetic diversity of PVY viruses in this country.
publishDate 2017
dc.date.none.fl_str_mv 2017-06
2018-08-23T13:27:49Z
2018-08-23T13:27:49Z
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv https://link.springer.com/article/10.1007/s12230-016-9564-y
http://hdl.handle.net/20.500.12123/3163
1099-209X
1874-9380
https://doi.org/10.1007/s12230-016-9564-y
url https://link.springer.com/article/10.1007/s12230-016-9564-y
http://hdl.handle.net/20.500.12123/3163
https://doi.org/10.1007/s12230-016-9564-y
identifier_str_mv 1099-209X
1874-9380
dc.language.none.fl_str_mv eng
language eng
dc.rights.none.fl_str_mv info:eu-repo/semantics/restrictedAccess
eu_rights_str_mv restrictedAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Springer Netherlands
publisher.none.fl_str_mv Springer Netherlands
dc.source.none.fl_str_mv American Journal of Potato Research 94 (3) : 275-282 (June 2017 )
reponame:INTA Digital (INTA)
instname:Instituto Nacional de Tecnología Agropecuaria
reponame_str INTA Digital (INTA)
collection INTA Digital (INTA)
instname_str Instituto Nacional de Tecnología Agropecuaria
repository.name.fl_str_mv INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuaria
repository.mail.fl_str_mv tripaldi.nicolas@inta.gob.ar
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score 12.623145

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