Cathelicidin modulates synthesis of Toll-like Receptors (TLRs) 4 and 9 in colonic epithelium

Autores
Marín, Maia Solange; Holani, Ravi; Shah, Chaitanya B.; Odeon, Anselmo Carlos; Cobo, Eduardo Rubén
Año de publicación
2017
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Cathelicidin are innate antimicrobial peptides with broad immunomodulatory functions; however, their role in regulating intestinal defenses is not well characterized. This study aimed to investigate the role of cathelicidin modulating expression of Toll-like receptors (TLRs) 4 and 9 in colonic epithelium in response to bacterial patterns. We demonstrated herein that intestinal epithelial cells, when primed by bacterial lipopolysaccharide (LPS), responded to cathelicidin by increased transcription and protein synthesis of TLR4. This cathelicidin-induced response required the interaction of LPS-TLR4 and activation of MAPK signalling pathways. However, cathelicidin blocked TLR9 responses induced by TLR9 ligand CpG oligodeoxynucleotide (CpG ODN) in these colonic epithelial cells. Modulations of TLRs triggered by cathelicidin in intestinal epithelium occurred mainly in the apical compartment of intestinal cells. Activation of TLR4 by ligands in combination with cathelicidin promoted CXCL8 chemokine secretion and epithelial antimicrobial defenses against Escherichia coli. We concluded that cathelicidin selectively modulated synthesis of TLR4 and 9 in intestinal epithelium, but only when cells were exposed to virulence factors, mostly from apical surfaces. Enhanced TLR4 expression promoted by cathelicidin in intestinal epithelium may be crucial for controlling enteric infectious diseases.
EEA Balcarce
Fil: Marin, Maia Solange. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Holani, Ravi. University of Calgary. Faculty of Veterinary Medicine. Production Animal Health; Canadá
Fil: Shah, Chaitanya B. University of Calgary. Bachelor of Health Sciences; Canadá
Fil: Odeon, Anselmo Carlos. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina
Fil: Cobo, Eduardo R. University of Calgary. Faculty of Veterinary Medicine. Production Animal Health; Canadá
Fuente
Molecular immunology 91 : 249-258. (November 2017)
Materia
Enfermedades de los Animales
Encefalitis
Antimicrobianos
Escherichia coli
Animal Diseases
Encephalitis
Antimicrobials
Nivel de accesibilidad
acceso restringido
Condiciones de uso
Repositorio
INTA Digital (INTA)
Institución
Instituto Nacional de Tecnología Agropecuaria
OAI Identificador
oai:localhost:20.500.12123/2018
Acceder
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oai_identifier_str oai:localhost:20.500.12123/2018
network_acronym_str INTADig
repository_id_str l
network_name_str INTA Digital (INTA)
spelling Cathelicidin modulates synthesis of Toll-like Receptors (TLRs) 4 and 9 in colonic epitheliumMarín, Maia SolangeHolani, RaviShah, Chaitanya B.Odeon, Anselmo CarlosCobo, Eduardo RubénEnfermedades de los AnimalesEncefalitisAntimicrobianosEscherichia coliAnimal DiseasesEncephalitisAntimicrobialsCathelicidin are innate antimicrobial peptides with broad immunomodulatory functions; however, their role in regulating intestinal defenses is not well characterized. This study aimed to investigate the role of cathelicidin modulating expression of Toll-like receptors (TLRs) 4 and 9 in colonic epithelium in response to bacterial patterns. We demonstrated herein that intestinal epithelial cells, when primed by bacterial lipopolysaccharide (LPS), responded to cathelicidin by increased transcription and protein synthesis of TLR4. This cathelicidin-induced response required the interaction of LPS-TLR4 and activation of MAPK signalling pathways. However, cathelicidin blocked TLR9 responses induced by TLR9 ligand CpG oligodeoxynucleotide (CpG ODN) in these colonic epithelial cells. Modulations of TLRs triggered by cathelicidin in intestinal epithelium occurred mainly in the apical compartment of intestinal cells. Activation of TLR4 by ligands in combination with cathelicidin promoted CXCL8 chemokine secretion and epithelial antimicrobial defenses against Escherichia coli. We concluded that cathelicidin selectively modulated synthesis of TLR4 and 9 in intestinal epithelium, but only when cells were exposed to virulence factors, mostly from apical surfaces. Enhanced TLR4 expression promoted by cathelicidin in intestinal epithelium may be crucial for controlling enteric infectious diseases.EEA BalcarceFil: Marin, Maia Solange. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Holani, Ravi. University of Calgary. Faculty of Veterinary Medicine. Production Animal Health; CanadáFil: Shah, Chaitanya B. University of Calgary. Bachelor of Health Sciences; CanadáFil: Odeon, Anselmo Carlos. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; ArgentinaFil: Cobo, Eduardo R. University of Calgary. Faculty of Veterinary Medicine. Production Animal Health; Canadá2018-03-13T17:16:25Z2018-03-13T17:16:25Z2017-11info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://hdl.handle.net/20.500.12123/2018https://www.sciencedirect.com/science/article/pii/S0161589017305102?via%3Dihub#!0161-5890https://doi.org/10.1016/j.molimm.2017.09.011Molecular immunology 91 : 249-258. (November 2017)reponame:INTA Digital (INTA)instname:Instituto Nacional de Tecnología Agropecuariaenginfo:eu-repo/semantics/restrictedAccess2025-10-23T11:16:28Zoai:localhost:20.500.12123/2018instacron:INTAInstitucionalhttp://repositorio.inta.gob.ar/Organismo científico-tecnológicoNo correspondehttp://repositorio.inta.gob.ar/oai/requesttripaldi.nicolas@inta.gob.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:l2025-10-23 11:16:29.023INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuariafalse
dc.title.none.fl_str_mv Cathelicidin modulates synthesis of Toll-like Receptors (TLRs) 4 and 9 in colonic epithelium
title Cathelicidin modulates synthesis of Toll-like Receptors (TLRs) 4 and 9 in colonic epithelium
spellingShingle Cathelicidin modulates synthesis of Toll-like Receptors (TLRs) 4 and 9 in colonic epithelium
Marín, Maia Solange
Enfermedades de los Animales
Encefalitis
Antimicrobianos
Escherichia coli
Animal Diseases
Encephalitis
Antimicrobials
title_short Cathelicidin modulates synthesis of Toll-like Receptors (TLRs) 4 and 9 in colonic epithelium
title_full Cathelicidin modulates synthesis of Toll-like Receptors (TLRs) 4 and 9 in colonic epithelium
title_fullStr Cathelicidin modulates synthesis of Toll-like Receptors (TLRs) 4 and 9 in colonic epithelium
title_full_unstemmed Cathelicidin modulates synthesis of Toll-like Receptors (TLRs) 4 and 9 in colonic epithelium
title_sort Cathelicidin modulates synthesis of Toll-like Receptors (TLRs) 4 and 9 in colonic epithelium
dc.creator.none.fl_str_mv Marín, Maia Solange
Holani, Ravi
Shah, Chaitanya B.
Odeon, Anselmo Carlos
Cobo, Eduardo Rubén
author Marín, Maia Solange
author_facet Marín, Maia Solange
Holani, Ravi
Shah, Chaitanya B.
Odeon, Anselmo Carlos
Cobo, Eduardo Rubén
author_role author
author2 Holani, Ravi
Shah, Chaitanya B.
Odeon, Anselmo Carlos
Cobo, Eduardo Rubén
author2_role author
author
author
author
dc.subject.none.fl_str_mv Enfermedades de los Animales
Encefalitis
Antimicrobianos
Escherichia coli
Animal Diseases
Encephalitis
Antimicrobials
topic Enfermedades de los Animales
Encefalitis
Antimicrobianos
Escherichia coli
Animal Diseases
Encephalitis
Antimicrobials
dc.description.none.fl_txt_mv Cathelicidin are innate antimicrobial peptides with broad immunomodulatory functions; however, their role in regulating intestinal defenses is not well characterized. This study aimed to investigate the role of cathelicidin modulating expression of Toll-like receptors (TLRs) 4 and 9 in colonic epithelium in response to bacterial patterns. We demonstrated herein that intestinal epithelial cells, when primed by bacterial lipopolysaccharide (LPS), responded to cathelicidin by increased transcription and protein synthesis of TLR4. This cathelicidin-induced response required the interaction of LPS-TLR4 and activation of MAPK signalling pathways. However, cathelicidin blocked TLR9 responses induced by TLR9 ligand CpG oligodeoxynucleotide (CpG ODN) in these colonic epithelial cells. Modulations of TLRs triggered by cathelicidin in intestinal epithelium occurred mainly in the apical compartment of intestinal cells. Activation of TLR4 by ligands in combination with cathelicidin promoted CXCL8 chemokine secretion and epithelial antimicrobial defenses against Escherichia coli. We concluded that cathelicidin selectively modulated synthesis of TLR4 and 9 in intestinal epithelium, but only when cells were exposed to virulence factors, mostly from apical surfaces. Enhanced TLR4 expression promoted by cathelicidin in intestinal epithelium may be crucial for controlling enteric infectious diseases.
EEA Balcarce
Fil: Marin, Maia Solange. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Holani, Ravi. University of Calgary. Faculty of Veterinary Medicine. Production Animal Health; Canadá
Fil: Shah, Chaitanya B. University of Calgary. Bachelor of Health Sciences; Canadá
Fil: Odeon, Anselmo Carlos. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina
Fil: Cobo, Eduardo R. University of Calgary. Faculty of Veterinary Medicine. Production Animal Health; Canadá
description Cathelicidin are innate antimicrobial peptides with broad immunomodulatory functions; however, their role in regulating intestinal defenses is not well characterized. This study aimed to investigate the role of cathelicidin modulating expression of Toll-like receptors (TLRs) 4 and 9 in colonic epithelium in response to bacterial patterns. We demonstrated herein that intestinal epithelial cells, when primed by bacterial lipopolysaccharide (LPS), responded to cathelicidin by increased transcription and protein synthesis of TLR4. This cathelicidin-induced response required the interaction of LPS-TLR4 and activation of MAPK signalling pathways. However, cathelicidin blocked TLR9 responses induced by TLR9 ligand CpG oligodeoxynucleotide (CpG ODN) in these colonic epithelial cells. Modulations of TLRs triggered by cathelicidin in intestinal epithelium occurred mainly in the apical compartment of intestinal cells. Activation of TLR4 by ligands in combination with cathelicidin promoted CXCL8 chemokine secretion and epithelial antimicrobial defenses against Escherichia coli. We concluded that cathelicidin selectively modulated synthesis of TLR4 and 9 in intestinal epithelium, but only when cells were exposed to virulence factors, mostly from apical surfaces. Enhanced TLR4 expression promoted by cathelicidin in intestinal epithelium may be crucial for controlling enteric infectious diseases.
publishDate 2017
dc.date.none.fl_str_mv 2017-11
2018-03-13T17:16:25Z
2018-03-13T17:16:25Z
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/20.500.12123/2018
https://www.sciencedirect.com/science/article/pii/S0161589017305102?via%3Dihub#!
0161-5890
https://doi.org/10.1016/j.molimm.2017.09.011
url http://hdl.handle.net/20.500.12123/2018
https://www.sciencedirect.com/science/article/pii/S0161589017305102?via%3Dihub#!
https://doi.org/10.1016/j.molimm.2017.09.011
identifier_str_mv 0161-5890
dc.language.none.fl_str_mv eng
language eng
dc.rights.none.fl_str_mv info:eu-repo/semantics/restrictedAccess
eu_rights_str_mv restrictedAccess
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv Molecular immunology 91 : 249-258. (November 2017)
reponame:INTA Digital (INTA)
instname:Instituto Nacional de Tecnología Agropecuaria
reponame_str INTA Digital (INTA)
collection INTA Digital (INTA)
instname_str Instituto Nacional de Tecnología Agropecuaria
repository.name.fl_str_mv INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuaria
repository.mail.fl_str_mv tripaldi.nicolas@inta.gob.ar
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score 12.982451

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