Molecular mapping of vernalization requirement and fertility restoration genes in carrot

Autores
Alessandro, Maria Soledad; Galmarini, Claudio Romulo; Iorizzo, Massimo; Simon, Philipp W.
Año de publicación
2013
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Carrot (Daucus carota L.) is a cool-season vegetable normally classified as a biennial species, requiring vernalization to induce flowering. Nevertheless, some cultivars adapted to warmer climates require less vernalization and can be classified as annual. Most modern carrot cultivars are hybrids which rely upon cytoplasmic male-sterility for commercial production. One major gene controlling floral initiation and several genes restoring male fertility have been reported but none have been mapped. The objective of the present work was to develop the first linkage map of carrot locating the genomic regions that control vernalization response and fertility restoration. Using an F2 progeny, derived from the intercross between the annual cultivar ‘Criolla INTA’ and a petaloid male sterile biennial carrot evaluated over 2 years, both early flowering habit, which we name Vrn1, and restoration of petaloid cytoplasmic male sterility, which we name Rf1, were found to be dominant traits conditioned by single genes. On a map of 355 markers covering all 9 chromosomes with a total map length of 669 cM and an average marker-to-marker distance of 1.88 cM, Vrn1 mapped to chromosome 2 with flanking markers at 0.70 and 0.46 cM, and Rf1 mapped to chromosome 9 with flanking markers at 4.38 and 1.12 cM. These are the first two reproductive traits mapped in the carrot genome, and their map location and flanking markers provide valuable tools for studying traits important for carrot domestication and reproductive biology, as well as facilitating carrot breeding.
EEA La Consulta
Fil: Alessandro, Maria Soledad. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria La Consulta; Argentina
Fil: Galmarini, Claudio Romulo. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria La Consulta; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Cuyo. Facultad de Ciencias Agrarias; Argentina
Fil: Iorizzo, Massimo. University of Wisconsin. Department of Horticulture; Estados Unidos.
Fil: Simon, Philipp W. United States Department of Agriculture–Agricultural Research Service. Vegetable Crops Research Unit; Estados Unidos
Fuente
Theoretical and Applied Genetics 126 (2) : 415–423 (February 2013)
Materia
Zanahoria
Daucus Carota
Marcadores Genéticos
Vernalización
Genética
Polimorfismo de Longitud de Fragmentos Amplificados
Carrots
Genetic Markers
Vernalization
Genetics
Amplified Fragment Length Polymorphism
Marcadores Moleculares
Nivel de accesibilidad
acceso restringido
Condiciones de uso
Repositorio
INTA Digital (INTA)
Institución
Instituto Nacional de Tecnología Agropecuaria
OAI Identificador
oai:localhost:20.500.12123/3253
Acceder
id INTADig_45a9bc82c7360524406f2db791fce07b
oai_identifier_str oai:localhost:20.500.12123/3253
network_acronym_str INTADig
repository_id_str l
network_name_str INTA Digital (INTA)
spelling Molecular mapping of vernalization requirement and fertility restoration genes in carrotAlessandro, Maria SoledadGalmarini, Claudio RomuloIorizzo, MassimoSimon, Philipp W.ZanahoriaDaucus CarotaMarcadores GenéticosVernalizaciónGenéticaPolimorfismo de Longitud de Fragmentos AmplificadosCarrotsGenetic MarkersVernalizationGeneticsAmplified Fragment Length PolymorphismMarcadores MolecularesCarrot (Daucus carota L.) is a cool-season vegetable normally classified as a biennial species, requiring vernalization to induce flowering. Nevertheless, some cultivars adapted to warmer climates require less vernalization and can be classified as annual. Most modern carrot cultivars are hybrids which rely upon cytoplasmic male-sterility for commercial production. One major gene controlling floral initiation and several genes restoring male fertility have been reported but none have been mapped. The objective of the present work was to develop the first linkage map of carrot locating the genomic regions that control vernalization response and fertility restoration. Using an F2 progeny, derived from the intercross between the annual cultivar ‘Criolla INTA’ and a petaloid male sterile biennial carrot evaluated over 2 years, both early flowering habit, which we name Vrn1, and restoration of petaloid cytoplasmic male sterility, which we name Rf1, were found to be dominant traits conditioned by single genes. On a map of 355 markers covering all 9 chromosomes with a total map length of 669 cM and an average marker-to-marker distance of 1.88 cM, Vrn1 mapped to chromosome 2 with flanking markers at 0.70 and 0.46 cM, and Rf1 mapped to chromosome 9 with flanking markers at 4.38 and 1.12 cM. These are the first two reproductive traits mapped in the carrot genome, and their map location and flanking markers provide valuable tools for studying traits important for carrot domestication and reproductive biology, as well as facilitating carrot breeding.EEA La ConsultaFil: Alessandro, Maria Soledad. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria La Consulta; ArgentinaFil: Galmarini, Claudio Romulo. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria La Consulta; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Cuyo. Facultad de Ciencias Agrarias; ArgentinaFil: Iorizzo, Massimo. University of Wisconsin. Department of Horticulture; Estados Unidos.Fil: Simon, Philipp W. United States Department of Agriculture–Agricultural Research Service. Vegetable Crops Research Unit; Estados Unidos2018-08-30T14:10:57Z2018-08-30T14:10:57Z2013-02info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttps://link.springer.com/article/10.1007/s00122-012-1989-1http://hdl.handle.net/20.500.12123/32530040-57521432-2242https://doi.org/10.1007/s00122-012-1989-1Theoretical and Applied Genetics 126 (2) : 415–423 (February 2013)reponame:INTA Digital (INTA)instname:Instituto Nacional de Tecnología Agropecuariaenginfo:eu-repo/semantics/restrictedAccess2025-10-16T09:29:17Zoai:localhost:20.500.12123/3253instacron:INTAInstitucionalhttp://repositorio.inta.gob.ar/Organismo científico-tecnológicoNo correspondehttp://repositorio.inta.gob.ar/oai/requesttripaldi.nicolas@inta.gob.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:l2025-10-16 09:29:17.683INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuariafalse
dc.title.none.fl_str_mv Molecular mapping of vernalization requirement and fertility restoration genes in carrot
title Molecular mapping of vernalization requirement and fertility restoration genes in carrot
spellingShingle Molecular mapping of vernalization requirement and fertility restoration genes in carrot
Alessandro, Maria Soledad
Zanahoria
Daucus Carota
Marcadores Genéticos
Vernalización
Genética
Polimorfismo de Longitud de Fragmentos Amplificados
Carrots
Genetic Markers
Vernalization
Genetics
Amplified Fragment Length Polymorphism
Marcadores Moleculares
title_short Molecular mapping of vernalization requirement and fertility restoration genes in carrot
title_full Molecular mapping of vernalization requirement and fertility restoration genes in carrot
title_fullStr Molecular mapping of vernalization requirement and fertility restoration genes in carrot
title_full_unstemmed Molecular mapping of vernalization requirement and fertility restoration genes in carrot
title_sort Molecular mapping of vernalization requirement and fertility restoration genes in carrot
dc.creator.none.fl_str_mv Alessandro, Maria Soledad
Galmarini, Claudio Romulo
Iorizzo, Massimo
Simon, Philipp W.
author Alessandro, Maria Soledad
author_facet Alessandro, Maria Soledad
Galmarini, Claudio Romulo
Iorizzo, Massimo
Simon, Philipp W.
author_role author
author2 Galmarini, Claudio Romulo
Iorizzo, Massimo
Simon, Philipp W.
author2_role author
author
author
dc.subject.none.fl_str_mv Zanahoria
Daucus Carota
Marcadores Genéticos
Vernalización
Genética
Polimorfismo de Longitud de Fragmentos Amplificados
Carrots
Genetic Markers
Vernalization
Genetics
Amplified Fragment Length Polymorphism
Marcadores Moleculares
topic Zanahoria
Daucus Carota
Marcadores Genéticos
Vernalización
Genética
Polimorfismo de Longitud de Fragmentos Amplificados
Carrots
Genetic Markers
Vernalization
Genetics
Amplified Fragment Length Polymorphism
Marcadores Moleculares
dc.description.none.fl_txt_mv Carrot (Daucus carota L.) is a cool-season vegetable normally classified as a biennial species, requiring vernalization to induce flowering. Nevertheless, some cultivars adapted to warmer climates require less vernalization and can be classified as annual. Most modern carrot cultivars are hybrids which rely upon cytoplasmic male-sterility for commercial production. One major gene controlling floral initiation and several genes restoring male fertility have been reported but none have been mapped. The objective of the present work was to develop the first linkage map of carrot locating the genomic regions that control vernalization response and fertility restoration. Using an F2 progeny, derived from the intercross between the annual cultivar ‘Criolla INTA’ and a petaloid male sterile biennial carrot evaluated over 2 years, both early flowering habit, which we name Vrn1, and restoration of petaloid cytoplasmic male sterility, which we name Rf1, were found to be dominant traits conditioned by single genes. On a map of 355 markers covering all 9 chromosomes with a total map length of 669 cM and an average marker-to-marker distance of 1.88 cM, Vrn1 mapped to chromosome 2 with flanking markers at 0.70 and 0.46 cM, and Rf1 mapped to chromosome 9 with flanking markers at 4.38 and 1.12 cM. These are the first two reproductive traits mapped in the carrot genome, and their map location and flanking markers provide valuable tools for studying traits important for carrot domestication and reproductive biology, as well as facilitating carrot breeding.
EEA La Consulta
Fil: Alessandro, Maria Soledad. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria La Consulta; Argentina
Fil: Galmarini, Claudio Romulo. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria La Consulta; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Cuyo. Facultad de Ciencias Agrarias; Argentina
Fil: Iorizzo, Massimo. University of Wisconsin. Department of Horticulture; Estados Unidos.
Fil: Simon, Philipp W. United States Department of Agriculture–Agricultural Research Service. Vegetable Crops Research Unit; Estados Unidos
description Carrot (Daucus carota L.) is a cool-season vegetable normally classified as a biennial species, requiring vernalization to induce flowering. Nevertheless, some cultivars adapted to warmer climates require less vernalization and can be classified as annual. Most modern carrot cultivars are hybrids which rely upon cytoplasmic male-sterility for commercial production. One major gene controlling floral initiation and several genes restoring male fertility have been reported but none have been mapped. The objective of the present work was to develop the first linkage map of carrot locating the genomic regions that control vernalization response and fertility restoration. Using an F2 progeny, derived from the intercross between the annual cultivar ‘Criolla INTA’ and a petaloid male sterile biennial carrot evaluated over 2 years, both early flowering habit, which we name Vrn1, and restoration of petaloid cytoplasmic male sterility, which we name Rf1, were found to be dominant traits conditioned by single genes. On a map of 355 markers covering all 9 chromosomes with a total map length of 669 cM and an average marker-to-marker distance of 1.88 cM, Vrn1 mapped to chromosome 2 with flanking markers at 0.70 and 0.46 cM, and Rf1 mapped to chromosome 9 with flanking markers at 4.38 and 1.12 cM. These are the first two reproductive traits mapped in the carrot genome, and their map location and flanking markers provide valuable tools for studying traits important for carrot domestication and reproductive biology, as well as facilitating carrot breeding.
publishDate 2013
dc.date.none.fl_str_mv 2013-02
2018-08-30T14:10:57Z
2018-08-30T14:10:57Z
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv https://link.springer.com/article/10.1007/s00122-012-1989-1
http://hdl.handle.net/20.500.12123/3253
0040-5752
1432-2242
https://doi.org/10.1007/s00122-012-1989-1
url https://link.springer.com/article/10.1007/s00122-012-1989-1
http://hdl.handle.net/20.500.12123/3253
https://doi.org/10.1007/s00122-012-1989-1
identifier_str_mv 0040-5752
1432-2242
dc.language.none.fl_str_mv eng
language eng
dc.rights.none.fl_str_mv info:eu-repo/semantics/restrictedAccess
eu_rights_str_mv restrictedAccess
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv Theoretical and Applied Genetics 126 (2) : 415–423 (February 2013)
reponame:INTA Digital (INTA)
instname:Instituto Nacional de Tecnología Agropecuaria
reponame_str INTA Digital (INTA)
collection INTA Digital (INTA)
instname_str Instituto Nacional de Tecnología Agropecuaria
repository.name.fl_str_mv INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuaria
repository.mail.fl_str_mv tripaldi.nicolas@inta.gob.ar
_version_ 1846143504792682496
score 12.712165

Publicaciones similares