Epidemiological characterization of isolates of Salmonella enterica and shiga toxin-producing Escherichia coli from backyard production system animals in the Valparaíso and metropo...

Autores: Urzúa-Encina, Constanza; Fernández-Sanhueza, Bastián; Pavez-Muñoz, Erika; Ramírez-Toloza, Galia; Tomazic, Mariela Luján; Rodriguez, Anabel Elisa; Alegría-Morán, Raúl
Año de publicación: 2023
Idioma: inglés
Tipo de recurso: artículo
Estado: versión publicada
Descripción: Backyard production systems (BPS) are distributed worldwide, rearing animals recognized as reservoirs of Salmonella enterica and Shiga toxin-producing Escherichia coli (STEC), both zoonotic pathogens. The aim of this study was to characterize isolates of both pathogens obtained from animals raised in BPS from two central Chile regions. The presence of pathogens was determined by bacterial culture and confirmatory PCR for each sampled BPS, calculating positivity rates. Multivariate logistic regression was used to determine risk factors. Additionally, phenotypic antimicrobial resistance was determined. A positivity rate of 2.88% for S. enterica and 14.39% for STEC was determined for the complete study region (Valparaíso and Metropolitana regions). Risk factor analysis suggests that the presence of ruminants (OR = 1.03; 95% CI = 1.002–1.075) increases the risk of STEC-positive BPS, and the presence of ruminants (OR = 1.05; 95% CI = 1.002–1.075) and the animal handlers being exclusively women (OR = 3.54; 95% CI = 1.029–12.193) increase the risk for S. enterica/STEC positivity. Eighty percent of S. enterica isolates were multidrug resistant, and all STEC were resistant to Cephalexin. This study evidences the circulation of multidrug-resistant zoonotic bacterial strains in animals kept in BPS and the presence of factors that modify the risk of BPS positivity for both pathogens.
Instituto de Patobiología
Fil: Urzúa-Encina, Constanza. Universidad de Chile. Facultad de Ciencias Veterinarias y Pecuarias. Departamento de Medicina Preventiva Animal; Chile
Fil: Urzúa-Encina, Constanza. Universidad de Chile. Facultad de Ciencias Veterinarias y Pecuarias. Laboratorio Centralizado de Investigación Veterinaria; Chile
Fil: Fernández-Sanhueza, Bastián. Universidad de Chile. Facultad de Ciencias Veterinarias y Pecuarias. Departamento de Medicina Preventiva Animal; Chile
Fil: Fernández-Sanhueza, Bastián. Universidad de Chile. Facultad de Ciencias Veterinarias y Pecuarias. Laboratorio Centralizado de Investigación Veterinaria; Chile
Fil: Fernández-Sanhueza, Bastián. Universidad Santo Tomás. Facultad de Recursos Naturales y Medicina Veterinaria. Escuela de Medicina Veterinaria, Sede Santiago; Chile
Fil: Pavez-Muñoz, Erika. Universidad de Chile. Facultad de Ciencias Veterinarias y Pecuarias. Departamento de Medicina Preventiva Animal; Chile
Fil: Pavez-Muñoz, Erika. Universidad de Chile. Facultad de Ciencias Veterinarias y Pecuarias. Laboratorio Centralizado de Investigación Veterinaria; Chile
Fil: Ramírez-Toloza, Galia. Universidad de Chile. Facultad de Ciencias Veterinarias y Pecuarias. Departamento de Medicina Preventiva Animal; Chile
Fil: Ramírez-Toloza, Galia. Universidad de Chile. Facultad de Ciencias Veterinarias y Pecuarias. Laboratorio Centralizado de Investigación Veterinaria; Chile
Fil: Tomazic, Mariela Luján. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología Veterinaria; Argentina
Fil: Tomazic, Mariela Luján. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Tomazic, Mariela Luján. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica; Argentina
Fil: Rodriguez, Anabel Elisa. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología Veterinaria; Argentina
Fil: Rodriguez, Anabel Elisa. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Alegría-Morán, Raúl. Universidad Santo Tomás. Facultad de Recursos Naturales y Medicina Veterinaria. Escuela de Medicina Veterinaria, Sede Santiago; Chile
Fuente: Animals 13 (15) : 2444 (Agosto 2023)
Materia: Risk Factors
Antimicrobial Resistance
One Health Approach
Isolation
Factores de Riesgo
Resistencia a los Antimicrobianos
Enfoque Una Salud
Escherichia coli
Aislamiento
Salmonella enterica
Valparaíso, Chile
Productor de Toxina Shiga
Shiga toxin-producing
Nivel de accesibilidad: acceso abierto
Condiciones de uso: http://creativecommons.org/licenses/by-nc-sa/4.0/
Repositorio
Institución: Instituto Nacional de Tecnología Agropecuaria
OAI Identificador: oai:localhost:20.500.12123/18505

Acceder

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spelling	Epidemiological characterization of isolates of Salmonella enterica and shiga toxin-producing Escherichia coli from backyard production system animals in the Valparaíso and metropolitana regionsUrzúa-Encina, ConstanzaFernández-Sanhueza, BastiánPavez-Muñoz, ErikaRamírez-Toloza, GaliaTomazic, Mariela LujánRodriguez, Anabel ElisaAlegría-Morán, RaúlRisk FactorsAntimicrobial ResistanceOne Health ApproachIsolationFactores de RiesgoResistencia a los AntimicrobianosEnfoque Una SaludEscherichia coliAislamientoSalmonella entericaValparaíso, ChileProductor de Toxina ShigaShiga toxin-producingBackyard production systems (BPS) are distributed worldwide, rearing animals recognized as reservoirs of Salmonella enterica and Shiga toxin-producing Escherichia coli (STEC), both zoonotic pathogens. The aim of this study was to characterize isolates of both pathogens obtained from animals raised in BPS from two central Chile regions. The presence of pathogens was determined by bacterial culture and confirmatory PCR for each sampled BPS, calculating positivity rates. Multivariate logistic regression was used to determine risk factors. Additionally, phenotypic antimicrobial resistance was determined. A positivity rate of 2.88% for S. enterica and 14.39% for STEC was determined for the complete study region (Valparaíso and Metropolitana regions). Risk factor analysis suggests that the presence of ruminants (OR = 1.03; 95% CI = 1.002–1.075) increases the risk of STEC-positive BPS, and the presence of ruminants (OR = 1.05; 95% CI = 1.002–1.075) and the animal handlers being exclusively women (OR = 3.54; 95% CI = 1.029–12.193) increase the risk for S. enterica/STEC positivity. Eighty percent of S. enterica isolates were multidrug resistant, and all STEC were resistant to Cephalexin. This study evidences the circulation of multidrug-resistant zoonotic bacterial strains in animals kept in BPS and the presence of factors that modify the risk of BPS positivity for both pathogens.Instituto de PatobiologíaFil: Urzúa-Encina, Constanza. Universidad de Chile. Facultad de Ciencias Veterinarias y Pecuarias. Departamento de Medicina Preventiva Animal; ChileFil: Urzúa-Encina, Constanza. Universidad de Chile. Facultad de Ciencias Veterinarias y Pecuarias. Laboratorio Centralizado de Investigación Veterinaria; ChileFil: Fernández-Sanhueza, Bastián. Universidad de Chile. Facultad de Ciencias Veterinarias y Pecuarias. Departamento de Medicina Preventiva Animal; ChileFil: Fernández-Sanhueza, Bastián. Universidad de Chile. Facultad de Ciencias Veterinarias y Pecuarias. Laboratorio Centralizado de Investigación Veterinaria; ChileFil: Fernández-Sanhueza, Bastián. Universidad Santo Tomás. Facultad de Recursos Naturales y Medicina Veterinaria. Escuela de Medicina Veterinaria, Sede Santiago; ChileFil: Pavez-Muñoz, Erika. Universidad de Chile. Facultad de Ciencias Veterinarias y Pecuarias. Departamento de Medicina Preventiva Animal; ChileFil: Pavez-Muñoz, Erika. Universidad de Chile. Facultad de Ciencias Veterinarias y Pecuarias. Laboratorio Centralizado de Investigación Veterinaria; ChileFil: Ramírez-Toloza, Galia. Universidad de Chile. Facultad de Ciencias Veterinarias y Pecuarias. Departamento de Medicina Preventiva Animal; ChileFil: Ramírez-Toloza, Galia. Universidad de Chile. Facultad de Ciencias Veterinarias y Pecuarias. Laboratorio Centralizado de Investigación Veterinaria; ChileFil: Tomazic, Mariela Luján. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología Veterinaria; ArgentinaFil: Tomazic, Mariela Luján. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Tomazic, Mariela Luján. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica; ArgentinaFil: Rodriguez, Anabel Elisa. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología Veterinaria; ArgentinaFil: Rodriguez, Anabel Elisa. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Alegría-Morán, Raúl. Universidad Santo Tomás. Facultad de Recursos Naturales y Medicina Veterinaria. Escuela de Medicina Veterinaria, Sede Santiago; ChileMDPI2024-07-15T11:20:25Z2024-07-15T11:20:25Z2023-08info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://hdl.handle.net/20.500.12123/18505https://www.mdpi.com/2076-2615/13/15/24442076-2615https://doi.org/10.3390/ani13152444Animals 13 (15) : 2444 (Agosto 2023)reponame:INTA Digital (INTA)instname:Instituto Nacional de Tecnología Agropecuariaenginfo:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by-nc-sa/4.0/Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)2025-09-29T13:46:39Zoai:localhost:20.500.12123/18505instacron:INTAInstitucionalhttp://repositorio.inta.gob.ar/Organismo científico-tecnológicoNo correspondehttp://repositorio.inta.gob.ar/oai/requesttripaldi.nicolas@inta.gob.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:l2025-09-29 13:46:39.813INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuariafalse
dc.title.none.fl_str_mv	Epidemiological characterization of isolates of Salmonella enterica and shiga toxin-producing Escherichia coli from backyard production system animals in the Valparaíso and metropolitana regions
title	Epidemiological characterization of isolates of Salmonella enterica and shiga toxin-producing Escherichia coli from backyard production system animals in the Valparaíso and metropolitana regions
spellingShingle	Epidemiological characterization of isolates of Salmonella enterica and shiga toxin-producing Escherichia coli from backyard production system animals in the Valparaíso and metropolitana regions Urzúa-Encina, Constanza Risk Factors Antimicrobial Resistance One Health Approach Isolation Factores de Riesgo Resistencia a los Antimicrobianos Enfoque Una Salud Escherichia coli Aislamiento Salmonella enterica Valparaíso, Chile Productor de Toxina Shiga Shiga toxin-producing
title_short	Epidemiological characterization of isolates of Salmonella enterica and shiga toxin-producing Escherichia coli from backyard production system animals in the Valparaíso and metropolitana regions
title_full	Epidemiological characterization of isolates of Salmonella enterica and shiga toxin-producing Escherichia coli from backyard production system animals in the Valparaíso and metropolitana regions
title_fullStr	Epidemiological characterization of isolates of Salmonella enterica and shiga toxin-producing Escherichia coli from backyard production system animals in the Valparaíso and metropolitana regions
title_full_unstemmed	Epidemiological characterization of isolates of Salmonella enterica and shiga toxin-producing Escherichia coli from backyard production system animals in the Valparaíso and metropolitana regions
title_sort	Epidemiological characterization of isolates of Salmonella enterica and shiga toxin-producing Escherichia coli from backyard production system animals in the Valparaíso and metropolitana regions
dc.creator.none.fl_str_mv	Urzúa-Encina, Constanza Fernández-Sanhueza, Bastián Pavez-Muñoz, Erika Ramírez-Toloza, Galia Tomazic, Mariela Luján Rodriguez, Anabel Elisa Alegría-Morán, Raúl
author	Urzúa-Encina, Constanza
author_facet	Urzúa-Encina, Constanza Fernández-Sanhueza, Bastián Pavez-Muñoz, Erika Ramírez-Toloza, Galia Tomazic, Mariela Luján Rodriguez, Anabel Elisa Alegría-Morán, Raúl
author_role	author
author2	Fernández-Sanhueza, Bastián Pavez-Muñoz, Erika Ramírez-Toloza, Galia Tomazic, Mariela Luján Rodriguez, Anabel Elisa Alegría-Morán, Raúl
author2_role	author author author author author author
dc.subject.none.fl_str_mv	Risk Factors Antimicrobial Resistance One Health Approach Isolation Factores de Riesgo Resistencia a los Antimicrobianos Enfoque Una Salud Escherichia coli Aislamiento Salmonella enterica Valparaíso, Chile Productor de Toxina Shiga Shiga toxin-producing
topic	Risk Factors Antimicrobial Resistance One Health Approach Isolation Factores de Riesgo Resistencia a los Antimicrobianos Enfoque Una Salud Escherichia coli Aislamiento Salmonella enterica Valparaíso, Chile Productor de Toxina Shiga Shiga toxin-producing
dc.description.none.fl_txt_mv	Backyard production systems (BPS) are distributed worldwide, rearing animals recognized as reservoirs of Salmonella enterica and Shiga toxin-producing Escherichia coli (STEC), both zoonotic pathogens. The aim of this study was to characterize isolates of both pathogens obtained from animals raised in BPS from two central Chile regions. The presence of pathogens was determined by bacterial culture and confirmatory PCR for each sampled BPS, calculating positivity rates. Multivariate logistic regression was used to determine risk factors. Additionally, phenotypic antimicrobial resistance was determined. A positivity rate of 2.88% for S. enterica and 14.39% for STEC was determined for the complete study region (Valparaíso and Metropolitana regions). Risk factor analysis suggests that the presence of ruminants (OR = 1.03; 95% CI = 1.002–1.075) increases the risk of STEC-positive BPS, and the presence of ruminants (OR = 1.05; 95% CI = 1.002–1.075) and the animal handlers being exclusively women (OR = 3.54; 95% CI = 1.029–12.193) increase the risk for S. enterica/STEC positivity. Eighty percent of S. enterica isolates were multidrug resistant, and all STEC were resistant to Cephalexin. This study evidences the circulation of multidrug-resistant zoonotic bacterial strains in animals kept in BPS and the presence of factors that modify the risk of BPS positivity for both pathogens. Instituto de Patobiología Fil: Urzúa-Encina, Constanza. Universidad de Chile. Facultad de Ciencias Veterinarias y Pecuarias. Departamento de Medicina Preventiva Animal; Chile Fil: Urzúa-Encina, Constanza. Universidad de Chile. Facultad de Ciencias Veterinarias y Pecuarias. Laboratorio Centralizado de Investigación Veterinaria; Chile Fil: Fernández-Sanhueza, Bastián. Universidad de Chile. Facultad de Ciencias Veterinarias y Pecuarias. Departamento de Medicina Preventiva Animal; Chile Fil: Fernández-Sanhueza, Bastián. Universidad de Chile. Facultad de Ciencias Veterinarias y Pecuarias. Laboratorio Centralizado de Investigación Veterinaria; Chile Fil: Fernández-Sanhueza, Bastián. Universidad Santo Tomás. Facultad de Recursos Naturales y Medicina Veterinaria. Escuela de Medicina Veterinaria, Sede Santiago; Chile Fil: Pavez-Muñoz, Erika. Universidad de Chile. Facultad de Ciencias Veterinarias y Pecuarias. Departamento de Medicina Preventiva Animal; Chile Fil: Pavez-Muñoz, Erika. Universidad de Chile. Facultad de Ciencias Veterinarias y Pecuarias. Laboratorio Centralizado de Investigación Veterinaria; Chile Fil: Ramírez-Toloza, Galia. Universidad de Chile. Facultad de Ciencias Veterinarias y Pecuarias. Departamento de Medicina Preventiva Animal; Chile Fil: Ramírez-Toloza, Galia. Universidad de Chile. Facultad de Ciencias Veterinarias y Pecuarias. Laboratorio Centralizado de Investigación Veterinaria; Chile Fil: Tomazic, Mariela Luján. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología Veterinaria; Argentina Fil: Tomazic, Mariela Luján. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Tomazic, Mariela Luján. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica; Argentina Fil: Rodriguez, Anabel Elisa. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología Veterinaria; Argentina Fil: Rodriguez, Anabel Elisa. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Alegría-Morán, Raúl. Universidad Santo Tomás. Facultad de Recursos Naturales y Medicina Veterinaria. Escuela de Medicina Veterinaria, Sede Santiago; Chile
description	Backyard production systems (BPS) are distributed worldwide, rearing animals recognized as reservoirs of Salmonella enterica and Shiga toxin-producing Escherichia coli (STEC), both zoonotic pathogens. The aim of this study was to characterize isolates of both pathogens obtained from animals raised in BPS from two central Chile regions. The presence of pathogens was determined by bacterial culture and confirmatory PCR for each sampled BPS, calculating positivity rates. Multivariate logistic regression was used to determine risk factors. Additionally, phenotypic antimicrobial resistance was determined. A positivity rate of 2.88% for S. enterica and 14.39% for STEC was determined for the complete study region (Valparaíso and Metropolitana regions). Risk factor analysis suggests that the presence of ruminants (OR = 1.03; 95% CI = 1.002–1.075) increases the risk of STEC-positive BPS, and the presence of ruminants (OR = 1.05; 95% CI = 1.002–1.075) and the animal handlers being exclusively women (OR = 3.54; 95% CI = 1.029–12.193) increase the risk for S. enterica/STEC positivity. Eighty percent of S. enterica isolates were multidrug resistant, and all STEC were resistant to Cephalexin. This study evidences the circulation of multidrug-resistant zoonotic bacterial strains in animals kept in BPS and the presence of factors that modify the risk of BPS positivity for both pathogens.
publishDate	2023
dc.date.none.fl_str_mv	2023-08 2024-07-15T11:20:25Z 2024-07-15T11:20:25Z
dc.type.none.fl_str_mv	info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://purl.org/coar/resource_type/c_6501 info:ar-repo/semantics/articulo
format	article
status_str	publishedVersion
dc.identifier.none.fl_str_mv	http://hdl.handle.net/20.500.12123/18505 https://www.mdpi.com/2076-2615/13/15/2444 2076-2615 https://doi.org/10.3390/ani13152444
url	http://hdl.handle.net/20.500.12123/18505 https://www.mdpi.com/2076-2615/13/15/2444 https://doi.org/10.3390/ani13152444
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dc.language.none.fl_str_mv	eng
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dc.publisher.none.fl_str_mv	MDPI
publisher.none.fl_str_mv	MDPI
dc.source.none.fl_str_mv	Animals 13 (15) : 2444 (Agosto 2023) reponame:INTA Digital (INTA) instname:Instituto Nacional de Tecnología Agropecuaria
reponame_str	INTA Digital (INTA)
collection	INTA Digital (INTA)
instname_str	Instituto Nacional de Tecnología Agropecuaria
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repository.mail.fl_str_mv	tripaldi.nicolas@inta.gob.ar
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Epidemiological characterization of isolates of Salmonella enterica and shiga toxin-producing Escherichia coli from backyard production system animals in the Valparaíso and metropo...

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