Epidemiological Characterization of Isolates of Salmonella enterica and Shiga Toxin-Producing Escherichia coli from Backyard Production System Animals in the Valparaíso and Metropo...

Autores
Urzúa Encina, Constanza; Fernández Sanhueza, Bastián; Pavez Muñoz, Erika; Ramírez Toloza, Galia; Tomazic, Mariela Luján; Rodríguez, Anabel Elisa; Alegría Morán, Raúl
Año de publicación
2023
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Backyard production systems (BPS) are distributed worldwide, rearing animals recognized as reservoirs of Salmonella enterica and Shiga toxin-producing Escherichia coli (STEC), both zoonotic pathogens. The aim of this study was to characterize isolates of both pathogens obtained from animals raised in BPS from two central Chile regions. The presence of pathogens was determined by bacterial culture and confirmatory PCR for each sampled BPS, calculating positivity rates. Multivariate logistic regression was used to determine risk factors. Additionally, phenotypic antimicrobial resistance was determined. A positivity rate of 2.88% for S. enterica and 14.39% for STEC was determined for the complete study region (Valparaíso and Metropolitana regions). Risk factor analysis suggests that the presence of ruminants (OR = 1.03; 95% CI = 1.002–1.075) increases the risk of STEC-positive BPS, and the presence of ruminants (OR = 1.05; 95% CI = 1.002–1.075) and the animal handlers being exclusively women (OR = 3.54; 95% CI = 1.029–12.193) increase the risk for S. enterica/STEC positivity. Eighty percent of S. enterica isolates were multidrug resistant, and all STEC were resistant to Cephalexin. This study evidences the circulation of multidrug-resistant zoonotic bacterial strains in animals kept in BPS and the presence of factors that modify the risk of BPS positivity for both pathogens.
Fil: Urzúa Encina, Constanza. Universidad de Chile; Chile
Fil: Fernández Sanhueza, Bastián. Universidad de Chile; Chile. Universidad Santo Tomás;
Fil: Pavez Muñoz, Erika. Universidad de Chile; Chile
Fil: Ramírez Toloza, Galia. Universidad de Chile; Chile
Fil: Tomazic, Mariela Luján. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación En Ciencias Veterinarias y Agronómicas. Instituto de Patobiología Veterinaria. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Patobiología Veterinaria; Argentina
Fil: Rodríguez, Anabel Elisa. Universidad de Buenos Aires; Argentina
Fil: Alegría Morán, Raúl. Universidad Santo Tomás;
Materia
ANTIMICROBIAL RESISTANCE
BACKYARD PRODUCTION SYSTEM
ONE HEALTH
POSITIVITY RATE
RISK FACTORS
SALMONELLA ENTERICA
STEC
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/229167
Acceder
id CONICETDig_9ec4a2c559ef7d16e72e33a7c6914fe5
oai_identifier_str oai:ri.conicet.gov.ar:11336/229167
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Epidemiological Characterization of Isolates of Salmonella enterica and Shiga Toxin-Producing Escherichia coli from Backyard Production System Animals in the Valparaíso and Metropolitana RegionsUrzúa Encina, ConstanzaFernández Sanhueza, BastiánPavez Muñoz, ErikaRamírez Toloza, GaliaTomazic, Mariela LujánRodríguez, Anabel ElisaAlegría Morán, RaúlANTIMICROBIAL RESISTANCEBACKYARD PRODUCTION SYSTEMONE HEALTHPOSITIVITY RATERISK FACTORSSALMONELLA ENTERICASTEChttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Backyard production systems (BPS) are distributed worldwide, rearing animals recognized as reservoirs of Salmonella enterica and Shiga toxin-producing Escherichia coli (STEC), both zoonotic pathogens. The aim of this study was to characterize isolates of both pathogens obtained from animals raised in BPS from two central Chile regions. The presence of pathogens was determined by bacterial culture and confirmatory PCR for each sampled BPS, calculating positivity rates. Multivariate logistic regression was used to determine risk factors. Additionally, phenotypic antimicrobial resistance was determined. A positivity rate of 2.88% for S. enterica and 14.39% for STEC was determined for the complete study region (Valparaíso and Metropolitana regions). Risk factor analysis suggests that the presence of ruminants (OR = 1.03; 95% CI = 1.002–1.075) increases the risk of STEC-positive BPS, and the presence of ruminants (OR = 1.05; 95% CI = 1.002–1.075) and the animal handlers being exclusively women (OR = 3.54; 95% CI = 1.029–12.193) increase the risk for S. enterica/STEC positivity. Eighty percent of S. enterica isolates were multidrug resistant, and all STEC were resistant to Cephalexin. This study evidences the circulation of multidrug-resistant zoonotic bacterial strains in animals kept in BPS and the presence of factors that modify the risk of BPS positivity for both pathogens.Fil: Urzúa Encina, Constanza. Universidad de Chile; ChileFil: Fernández Sanhueza, Bastián. Universidad de Chile; Chile. Universidad Santo Tomás;Fil: Pavez Muñoz, Erika. Universidad de Chile; ChileFil: Ramírez Toloza, Galia. Universidad de Chile; ChileFil: Tomazic, Mariela Luján. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación En Ciencias Veterinarias y Agronómicas. Instituto de Patobiología Veterinaria. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Patobiología Veterinaria; ArgentinaFil: Rodríguez, Anabel Elisa. Universidad de Buenos Aires; ArgentinaFil: Alegría Morán, Raúl. Universidad Santo Tomás;Multidisciplinary Digital Publishing Institute2023-08info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/229167Urzúa Encina, Constanza; Fernández Sanhueza, Bastián; Pavez Muñoz, Erika; Ramírez Toloza, Galia; Tomazic, Mariela Luján; et al.; Epidemiological Characterization of Isolates of Salmonella enterica and Shiga Toxin-Producing Escherichia coli from Backyard Production System Animals in the Valparaíso and Metropolitana Regions; Multidisciplinary Digital Publishing Institute; Animals; 13; 15; 8-2023; 1-152076-2615CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.mdpi.com/2076-2615/13/15/2444/review_reportinfo:eu-repo/semantics/altIdentifier/doi/10.3390/ani13152444info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:32:50Zoai:ri.conicet.gov.ar:11336/229167instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:32:50.483CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Epidemiological Characterization of Isolates of Salmonella enterica and Shiga Toxin-Producing Escherichia coli from Backyard Production System Animals in the Valparaíso and Metropolitana Regions
title Epidemiological Characterization of Isolates of Salmonella enterica and Shiga Toxin-Producing Escherichia coli from Backyard Production System Animals in the Valparaíso and Metropolitana Regions
spellingShingle Epidemiological Characterization of Isolates of Salmonella enterica and Shiga Toxin-Producing Escherichia coli from Backyard Production System Animals in the Valparaíso and Metropolitana Regions
Urzúa Encina, Constanza
ANTIMICROBIAL RESISTANCE
BACKYARD PRODUCTION SYSTEM
ONE HEALTH
POSITIVITY RATE
RISK FACTORS
SALMONELLA ENTERICA
STEC
title_short Epidemiological Characterization of Isolates of Salmonella enterica and Shiga Toxin-Producing Escherichia coli from Backyard Production System Animals in the Valparaíso and Metropolitana Regions
title_full Epidemiological Characterization of Isolates of Salmonella enterica and Shiga Toxin-Producing Escherichia coli from Backyard Production System Animals in the Valparaíso and Metropolitana Regions
title_fullStr Epidemiological Characterization of Isolates of Salmonella enterica and Shiga Toxin-Producing Escherichia coli from Backyard Production System Animals in the Valparaíso and Metropolitana Regions
title_full_unstemmed Epidemiological Characterization of Isolates of Salmonella enterica and Shiga Toxin-Producing Escherichia coli from Backyard Production System Animals in the Valparaíso and Metropolitana Regions
title_sort Epidemiological Characterization of Isolates of Salmonella enterica and Shiga Toxin-Producing Escherichia coli from Backyard Production System Animals in the Valparaíso and Metropolitana Regions
dc.creator.none.fl_str_mv Urzúa Encina, Constanza
Fernández Sanhueza, Bastián
Pavez Muñoz, Erika
Ramírez Toloza, Galia
Tomazic, Mariela Luján
Rodríguez, Anabel Elisa
Alegría Morán, Raúl
author Urzúa Encina, Constanza
author_facet Urzúa Encina, Constanza
Fernández Sanhueza, Bastián
Pavez Muñoz, Erika
Ramírez Toloza, Galia
Tomazic, Mariela Luján
Rodríguez, Anabel Elisa
Alegría Morán, Raúl
author_role author
author2 Fernández Sanhueza, Bastián
Pavez Muñoz, Erika
Ramírez Toloza, Galia
Tomazic, Mariela Luján
Rodríguez, Anabel Elisa
Alegría Morán, Raúl
author2_role author
author
author
author
author
author
dc.subject.none.fl_str_mv ANTIMICROBIAL RESISTANCE
BACKYARD PRODUCTION SYSTEM
ONE HEALTH
POSITIVITY RATE
RISK FACTORS
SALMONELLA ENTERICA
STEC
topic ANTIMICROBIAL RESISTANCE
BACKYARD PRODUCTION SYSTEM
ONE HEALTH
POSITIVITY RATE
RISK FACTORS
SALMONELLA ENTERICA
STEC
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Backyard production systems (BPS) are distributed worldwide, rearing animals recognized as reservoirs of Salmonella enterica and Shiga toxin-producing Escherichia coli (STEC), both zoonotic pathogens. The aim of this study was to characterize isolates of both pathogens obtained from animals raised in BPS from two central Chile regions. The presence of pathogens was determined by bacterial culture and confirmatory PCR for each sampled BPS, calculating positivity rates. Multivariate logistic regression was used to determine risk factors. Additionally, phenotypic antimicrobial resistance was determined. A positivity rate of 2.88% for S. enterica and 14.39% for STEC was determined for the complete study region (Valparaíso and Metropolitana regions). Risk factor analysis suggests that the presence of ruminants (OR = 1.03; 95% CI = 1.002–1.075) increases the risk of STEC-positive BPS, and the presence of ruminants (OR = 1.05; 95% CI = 1.002–1.075) and the animal handlers being exclusively women (OR = 3.54; 95% CI = 1.029–12.193) increase the risk for S. enterica/STEC positivity. Eighty percent of S. enterica isolates were multidrug resistant, and all STEC were resistant to Cephalexin. This study evidences the circulation of multidrug-resistant zoonotic bacterial strains in animals kept in BPS and the presence of factors that modify the risk of BPS positivity for both pathogens.
Fil: Urzúa Encina, Constanza. Universidad de Chile; Chile
Fil: Fernández Sanhueza, Bastián. Universidad de Chile; Chile. Universidad Santo Tomás;
Fil: Pavez Muñoz, Erika. Universidad de Chile; Chile
Fil: Ramírez Toloza, Galia. Universidad de Chile; Chile
Fil: Tomazic, Mariela Luján. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación En Ciencias Veterinarias y Agronómicas. Instituto de Patobiología Veterinaria. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Patobiología Veterinaria; Argentina
Fil: Rodríguez, Anabel Elisa. Universidad de Buenos Aires; Argentina
Fil: Alegría Morán, Raúl. Universidad Santo Tomás;
description Backyard production systems (BPS) are distributed worldwide, rearing animals recognized as reservoirs of Salmonella enterica and Shiga toxin-producing Escherichia coli (STEC), both zoonotic pathogens. The aim of this study was to characterize isolates of both pathogens obtained from animals raised in BPS from two central Chile regions. The presence of pathogens was determined by bacterial culture and confirmatory PCR for each sampled BPS, calculating positivity rates. Multivariate logistic regression was used to determine risk factors. Additionally, phenotypic antimicrobial resistance was determined. A positivity rate of 2.88% for S. enterica and 14.39% for STEC was determined for the complete study region (Valparaíso and Metropolitana regions). Risk factor analysis suggests that the presence of ruminants (OR = 1.03; 95% CI = 1.002–1.075) increases the risk of STEC-positive BPS, and the presence of ruminants (OR = 1.05; 95% CI = 1.002–1.075) and the animal handlers being exclusively women (OR = 3.54; 95% CI = 1.029–12.193) increase the risk for S. enterica/STEC positivity. Eighty percent of S. enterica isolates were multidrug resistant, and all STEC were resistant to Cephalexin. This study evidences the circulation of multidrug-resistant zoonotic bacterial strains in animals kept in BPS and the presence of factors that modify the risk of BPS positivity for both pathogens.
publishDate 2023
dc.date.none.fl_str_mv 2023-08
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/229167
Urzúa Encina, Constanza; Fernández Sanhueza, Bastián; Pavez Muñoz, Erika; Ramírez Toloza, Galia; Tomazic, Mariela Luján; et al.; Epidemiological Characterization of Isolates of Salmonella enterica and Shiga Toxin-Producing Escherichia coli from Backyard Production System Animals in the Valparaíso and Metropolitana Regions; Multidisciplinary Digital Publishing Institute; Animals; 13; 15; 8-2023; 1-15
2076-2615
CONICET Digital
CONICET
url http://hdl.handle.net/11336/229167
identifier_str_mv Urzúa Encina, Constanza; Fernández Sanhueza, Bastián; Pavez Muñoz, Erika; Ramírez Toloza, Galia; Tomazic, Mariela Luján; et al.; Epidemiological Characterization of Isolates of Salmonella enterica and Shiga Toxin-Producing Escherichia coli from Backyard Production System Animals in the Valparaíso and Metropolitana Regions; Multidisciplinary Digital Publishing Institute; Animals; 13; 15; 8-2023; 1-15
2076-2615
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://www.mdpi.com/2076-2615/13/15/2444/review_report
info:eu-repo/semantics/altIdentifier/doi/10.3390/ani13152444
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Multidisciplinary Digital Publishing Institute
publisher.none.fl_str_mv Multidisciplinary Digital Publishing Institute
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.070432

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