Characterization of ZAT12 protein from Prunus persica: role in fruit chilling injury tolerance and identification of gene targets

Autores
Gismondi, Mauro; Strologo, Laura; Gabilondo, Julieta; Budde, Claudio Olaf; Drincovich, María Fabiana; Bustamante, Claudia Anabel
Año de publicación
2025
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
ZAT (zinc finger of Arabidopsis thaliana) proteins play roles in multiple abiotic stress tolerance in Arabidopsis and other species; however, there are few reports on these transcription factors (TFs) in fruit crops. This study aimed to evaluate PpZAT12, a C2H2 TF up-regulated in peach fruit by a heat treatment applied before postharvest cold storage for reducing chilling injury (CI) symptoms. Here, the expression of PpZAT12 in different tissues and fruits subjected to either postharvest heat or cold treatments, was evaluated in peach varieties with differential susceptibility to develop CI. PpZAT12 increased by cold storage in CI-resistant cultivars (‘Elegant Lady’ and ‘Rojo 2’), while it was not modified in a cultivar susceptible to develop CI (‘Flordaking’). Besides, we expressed PpZAT12 in Arabidopsis (35S::PpZAT12) and found that these plants show impaired plant growth and development, rendering small plants with senescence delay and aborted seeds. We applied a proteomic approach to decipher the peptides responding to PpZAT12 in Arabidopsis and found 348 differential expressed proteins (DEPs) relative to the wild type. Besides, comparing the DEPs between Arabidopsis plants expressing PpZAT12 or AtZAT12 (35S::AtZAT12) we found common and specific responses to these TFs. Based on the proteomic information obtained here and published data about AtZAT12, we searched ZAT12-targets in peach allowing the identification of a putative ZAT12 regulon in this species. The identified peach ZAT12-protein targets could underlie the differential susceptibility to CI among different peach varieties and can be used as future targets to improve adaptation to refrigeration in fleshy fruits.
EEA San Pedro
Fil: Gismondi, Mauro. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Centro de Estudios Fotosintéticos y Bioquímicos (CEFOBI); Argentina
Fil: Strologo, Laura. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Centro de Estudios Fotosintéticos y Bioquímicos (CEFOBI); Argentina
Fil: Gabilondo, Julieta. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria San Pedro; Argentina
Fil: Budde, Claudio Olaf. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria San Pedro; Argentina
Fil: Drincovich, María Fabiana. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Centro de Estudios Fotosintéticos y Bioquímicos (CEFOBI); Argentina
Fil: Drincovich, María Fabiana. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Bustamante, Claudia A. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Centro de Estudios Fotosintéticos y Bioquímicos (CEFOBI); Argentina
Fil: Bustamante, Claudia A. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fuente
Planta 261 : article number 14. (January 2025)
Materia
Prunus persica
Peaches
Arabidopsis thaliana
Cooling
Genetics
Resistance to Injurious Factors
Cold Tolerance
Durazno
Enfriamiento
Genética
Resistencia a Agentes Dañinos
Tolerancia al Frío
Chilling
ZAT12
Nivel de accesibilidad
acceso restringido
Condiciones de uso
http://creativecommons.org/licenses/by-nc-sa/4.0/
Repositorio
INTA Digital (INTA)
Institución
Instituto Nacional de Tecnología Agropecuaria
OAI Identificador
oai:localhost:20.500.12123/22107
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oai_identifier_str oai:localhost:20.500.12123/22107
network_acronym_str INTADig
repository_id_str l
network_name_str INTA Digital (INTA)
spelling Characterization of ZAT12 protein from Prunus persica: role in fruit chilling injury tolerance and identification of gene targetsGismondi, MauroStrologo, LauraGabilondo, JulietaBudde, Claudio OlafDrincovich, María FabianaBustamante, Claudia AnabelPrunus persicaPeachesArabidopsis thalianaCoolingGeneticsResistance to Injurious FactorsCold ToleranceDuraznoEnfriamientoGenéticaResistencia a Agentes DañinosTolerancia al FríoChillingZAT12ZAT (zinc finger of Arabidopsis thaliana) proteins play roles in multiple abiotic stress tolerance in Arabidopsis and other species; however, there are few reports on these transcription factors (TFs) in fruit crops. This study aimed to evaluate PpZAT12, a C2H2 TF up-regulated in peach fruit by a heat treatment applied before postharvest cold storage for reducing chilling injury (CI) symptoms. Here, the expression of PpZAT12 in different tissues and fruits subjected to either postharvest heat or cold treatments, was evaluated in peach varieties with differential susceptibility to develop CI. PpZAT12 increased by cold storage in CI-resistant cultivars (‘Elegant Lady’ and ‘Rojo 2’), while it was not modified in a cultivar susceptible to develop CI (‘Flordaking’). Besides, we expressed PpZAT12 in Arabidopsis (35S::PpZAT12) and found that these plants show impaired plant growth and development, rendering small plants with senescence delay and aborted seeds. We applied a proteomic approach to decipher the peptides responding to PpZAT12 in Arabidopsis and found 348 differential expressed proteins (DEPs) relative to the wild type. Besides, comparing the DEPs between Arabidopsis plants expressing PpZAT12 or AtZAT12 (35S::AtZAT12) we found common and specific responses to these TFs. Based on the proteomic information obtained here and published data about AtZAT12, we searched ZAT12-targets in peach allowing the identification of a putative ZAT12 regulon in this species. The identified peach ZAT12-protein targets could underlie the differential susceptibility to CI among different peach varieties and can be used as future targets to improve adaptation to refrigeration in fleshy fruits.EEA San PedroFil: Gismondi, Mauro. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Centro de Estudios Fotosintéticos y Bioquímicos (CEFOBI); ArgentinaFil: Strologo, Laura. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Centro de Estudios Fotosintéticos y Bioquímicos (CEFOBI); ArgentinaFil: Gabilondo, Julieta. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria San Pedro; ArgentinaFil: Budde, Claudio Olaf. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria San Pedro; ArgentinaFil: Drincovich, María Fabiana. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Centro de Estudios Fotosintéticos y Bioquímicos (CEFOBI); ArgentinaFil: Drincovich, María Fabiana. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Bustamante, Claudia A. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Centro de Estudios Fotosintéticos y Bioquímicos (CEFOBI); ArgentinaFil: Bustamante, Claudia A. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaSpringer2025-04-29T16:36:57Z2025-04-29T16:36:57Z2025-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://hdl.handle.net/20.500.12123/22107https://link.springer.com/article/10.1007/s00425-024-04593-x0032-09351432-2048https://doi.org/10.1007/s00425-024-04593-xPlanta 261 : article number 14. (January 2025)reponame:INTA Digital (INTA)instname:Instituto Nacional de Tecnología Agropecuariaenginfo:eu-repo/semantics/restrictedAccesshttp://creativecommons.org/licenses/by-nc-sa/4.0/Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)2026-01-08T10:40:05Zoai:localhost:20.500.12123/22107instacron:INTAInstitucionalhttp://repositorio.inta.gob.ar/Organismo científico-tecnológicoNo correspondehttp://repositorio.inta.gob.ar/oai/requesttripaldi.nicolas@inta.gob.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:l2026-01-08 10:40:05.367INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuariafalse
dc.title.none.fl_str_mv Characterization of ZAT12 protein from Prunus persica: role in fruit chilling injury tolerance and identification of gene targets
title Characterization of ZAT12 protein from Prunus persica: role in fruit chilling injury tolerance and identification of gene targets
spellingShingle Characterization of ZAT12 protein from Prunus persica: role in fruit chilling injury tolerance and identification of gene targets
Gismondi, Mauro
Prunus persica
Peaches
Arabidopsis thaliana
Cooling
Genetics
Resistance to Injurious Factors
Cold Tolerance
Durazno
Enfriamiento
Genética
Resistencia a Agentes Dañinos
Tolerancia al Frío
Chilling
ZAT12
title_short Characterization of ZAT12 protein from Prunus persica: role in fruit chilling injury tolerance and identification of gene targets
title_full Characterization of ZAT12 protein from Prunus persica: role in fruit chilling injury tolerance and identification of gene targets
title_fullStr Characterization of ZAT12 protein from Prunus persica: role in fruit chilling injury tolerance and identification of gene targets
title_full_unstemmed Characterization of ZAT12 protein from Prunus persica: role in fruit chilling injury tolerance and identification of gene targets
title_sort Characterization of ZAT12 protein from Prunus persica: role in fruit chilling injury tolerance and identification of gene targets
dc.creator.none.fl_str_mv Gismondi, Mauro
Strologo, Laura
Gabilondo, Julieta
Budde, Claudio Olaf
Drincovich, María Fabiana
Bustamante, Claudia Anabel
author Gismondi, Mauro
author_facet Gismondi, Mauro
Strologo, Laura
Gabilondo, Julieta
Budde, Claudio Olaf
Drincovich, María Fabiana
Bustamante, Claudia Anabel
author_role author
author2 Strologo, Laura
Gabilondo, Julieta
Budde, Claudio Olaf
Drincovich, María Fabiana
Bustamante, Claudia Anabel
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv Prunus persica
Peaches
Arabidopsis thaliana
Cooling
Genetics
Resistance to Injurious Factors
Cold Tolerance
Durazno
Enfriamiento
Genética
Resistencia a Agentes Dañinos
Tolerancia al Frío
Chilling
ZAT12
topic Prunus persica
Peaches
Arabidopsis thaliana
Cooling
Genetics
Resistance to Injurious Factors
Cold Tolerance
Durazno
Enfriamiento
Genética
Resistencia a Agentes Dañinos
Tolerancia al Frío
Chilling
ZAT12
dc.description.none.fl_txt_mv ZAT (zinc finger of Arabidopsis thaliana) proteins play roles in multiple abiotic stress tolerance in Arabidopsis and other species; however, there are few reports on these transcription factors (TFs) in fruit crops. This study aimed to evaluate PpZAT12, a C2H2 TF up-regulated in peach fruit by a heat treatment applied before postharvest cold storage for reducing chilling injury (CI) symptoms. Here, the expression of PpZAT12 in different tissues and fruits subjected to either postharvest heat or cold treatments, was evaluated in peach varieties with differential susceptibility to develop CI. PpZAT12 increased by cold storage in CI-resistant cultivars (‘Elegant Lady’ and ‘Rojo 2’), while it was not modified in a cultivar susceptible to develop CI (‘Flordaking’). Besides, we expressed PpZAT12 in Arabidopsis (35S::PpZAT12) and found that these plants show impaired plant growth and development, rendering small plants with senescence delay and aborted seeds. We applied a proteomic approach to decipher the peptides responding to PpZAT12 in Arabidopsis and found 348 differential expressed proteins (DEPs) relative to the wild type. Besides, comparing the DEPs between Arabidopsis plants expressing PpZAT12 or AtZAT12 (35S::AtZAT12) we found common and specific responses to these TFs. Based on the proteomic information obtained here and published data about AtZAT12, we searched ZAT12-targets in peach allowing the identification of a putative ZAT12 regulon in this species. The identified peach ZAT12-protein targets could underlie the differential susceptibility to CI among different peach varieties and can be used as future targets to improve adaptation to refrigeration in fleshy fruits.
EEA San Pedro
Fil: Gismondi, Mauro. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Centro de Estudios Fotosintéticos y Bioquímicos (CEFOBI); Argentina
Fil: Strologo, Laura. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Centro de Estudios Fotosintéticos y Bioquímicos (CEFOBI); Argentina
Fil: Gabilondo, Julieta. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria San Pedro; Argentina
Fil: Budde, Claudio Olaf. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria San Pedro; Argentina
Fil: Drincovich, María Fabiana. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Centro de Estudios Fotosintéticos y Bioquímicos (CEFOBI); Argentina
Fil: Drincovich, María Fabiana. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Bustamante, Claudia A. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Centro de Estudios Fotosintéticos y Bioquímicos (CEFOBI); Argentina
Fil: Bustamante, Claudia A. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
description ZAT (zinc finger of Arabidopsis thaliana) proteins play roles in multiple abiotic stress tolerance in Arabidopsis and other species; however, there are few reports on these transcription factors (TFs) in fruit crops. This study aimed to evaluate PpZAT12, a C2H2 TF up-regulated in peach fruit by a heat treatment applied before postharvest cold storage for reducing chilling injury (CI) symptoms. Here, the expression of PpZAT12 in different tissues and fruits subjected to either postharvest heat or cold treatments, was evaluated in peach varieties with differential susceptibility to develop CI. PpZAT12 increased by cold storage in CI-resistant cultivars (‘Elegant Lady’ and ‘Rojo 2’), while it was not modified in a cultivar susceptible to develop CI (‘Flordaking’). Besides, we expressed PpZAT12 in Arabidopsis (35S::PpZAT12) and found that these plants show impaired plant growth and development, rendering small plants with senescence delay and aborted seeds. We applied a proteomic approach to decipher the peptides responding to PpZAT12 in Arabidopsis and found 348 differential expressed proteins (DEPs) relative to the wild type. Besides, comparing the DEPs between Arabidopsis plants expressing PpZAT12 or AtZAT12 (35S::AtZAT12) we found common and specific responses to these TFs. Based on the proteomic information obtained here and published data about AtZAT12, we searched ZAT12-targets in peach allowing the identification of a putative ZAT12 regulon in this species. The identified peach ZAT12-protein targets could underlie the differential susceptibility to CI among different peach varieties and can be used as future targets to improve adaptation to refrigeration in fleshy fruits.
publishDate 2025
dc.date.none.fl_str_mv 2025-04-29T16:36:57Z
2025-04-29T16:36:57Z
2025-01
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/20.500.12123/22107
https://link.springer.com/article/10.1007/s00425-024-04593-x
0032-0935
1432-2048
https://doi.org/10.1007/s00425-024-04593-x
url http://hdl.handle.net/20.500.12123/22107
https://link.springer.com/article/10.1007/s00425-024-04593-x
https://doi.org/10.1007/s00425-024-04593-x
identifier_str_mv 0032-0935
1432-2048
dc.language.none.fl_str_mv eng
language eng
dc.rights.none.fl_str_mv info:eu-repo/semantics/restrictedAccess
http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
eu_rights_str_mv restrictedAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Springer
publisher.none.fl_str_mv Springer
dc.source.none.fl_str_mv Planta 261 : article number 14. (January 2025)
reponame:INTA Digital (INTA)
instname:Instituto Nacional de Tecnología Agropecuaria
reponame_str INTA Digital (INTA)
collection INTA Digital (INTA)
instname_str Instituto Nacional de Tecnología Agropecuaria
repository.name.fl_str_mv INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuaria
repository.mail.fl_str_mv tripaldi.nicolas@inta.gob.ar
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score 12.747614

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