Bicaudal C is required for the function of the follicular epithelium during oogenesis in Rhodnius prolixus

Autores
Pascual, Agustina; Vilardo, Emiliano Salvador; Taibo, Catalina Beatriz; Sabio Y Garcia, Julia Veronica; Rivera Pomar, Rolando
Año de publicación
2021
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The morphology and physiology of the oogenesis have been well studied in the vector of Chagas disease Rhodnius prolixus. However, the molecular interactions that regulate the process of egg formation, key for the reproductive cycle of the vector, is still largely unknown. In order to understand the molecular and cellular basis of the oogenesis, we examined the function of the gene Bicaudal C (BicC) during oogenesis and early development of R. prolixus. We show that R. prolixus BicC (Rp-BicC) gene is expressed in the germarium, with cytoplasmic distribution, as well as in the follicular epithelium of the developing oocytes. RNAi silencing of Rp-BicC resulted in sterile females that lay few, small, non-viable eggs. The ovaries are reduced in size and show a disarray of the follicular epithelium. This indicates that Rp-BicC has a central role in the regulation of oogenesis. Although the follicular cells are able to form the chorion, the uptake of vitelline by the oocytes is compromised. We show evidence that the polarity of the follicular epithelium and the endocytic pathway, which are crucial for the proper yolk deposition, are affected. This study provides insights into the molecular mechanisms underlying oocyte development and show that Rp-BicC is important for de developmental of the egg and, therefore, a key player in the reproduction of this insect.
Centro de Investigación en Ciencias Veterinarias y Agronómicas
Fil: Pascual, Agustina. Universidad Nacional del Norte de la Provincia de Buenos Aires. Centro de Bioinvestigaciones; Argentina
Fil: Pascual, Agustina. Comisión de Investigaciones Científicas de la Provincia de Buenos Aires. Centro de Bioinvestigaciones; Argentina
Fil: Vilardo, Emiliano Salvador. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Centro Regional de Estudios Genómicos; Argentina
Fil: Taibo, Catalina Beatriz. Instituto Nacional de Tecnología Agropecuaria (INTA). Centro de Investigación en Ciencias Veterinarias y Agronómicas. Laboratorio Integral de Microscopía; Argentina
Fil: Sabio Y Garcia, Julia Veronica. Instituto Nacional de Tecnología Agropecuaria (INTA). Centro de Investigación en Ciencias Veterinarias y Agronómicas. Laboratorio Integral de Microscopía; Argentina
Fil: Rivera Pomar, Rolando. Universidad Nacional del Norte de la Provincia de Buenos Aires. Centro de Bioinvestigaciones; Argentina
Fil: Rivera Pomar, Rolando. Comisión de Investigaciones Científicas de la Provincia de Buenos Aires. Centro de Bioinvestigaciones; Argentina
Fil: Rivera Pomar, Rolando. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Centro Regional de Estudios Genómicos; Argentina
Fuente
Development Genes and Evolution 231 : 33-45. (2021)
Materia
Oogénesis
Rhodnius prolixus
Desarrollo Embrionario
Vitelogénesis
Oogenesis
Embryonic Development
Vitellogenesis
Bicaudal C
Nivel de accesibilidad
acceso restringido
Condiciones de uso
Repositorio
INTA Digital (INTA)
Institución
Instituto Nacional de Tecnología Agropecuaria
OAI Identificador
oai:localhost:20.500.12123/10896
Acceder
id INTADig_40f722ebd85a1e78cb04152bdc0d0276
oai_identifier_str oai:localhost:20.500.12123/10896
network_acronym_str INTADig
repository_id_str l
network_name_str INTA Digital (INTA)
spelling Bicaudal C is required for the function of the follicular epithelium during oogenesis in Rhodnius prolixusPascual, AgustinaVilardo, Emiliano SalvadorTaibo, Catalina BeatrizSabio Y Garcia, Julia VeronicaRivera Pomar, RolandoOogénesisRhodnius prolixusDesarrollo EmbrionarioVitelogénesisOogenesisEmbryonic DevelopmentVitellogenesisBicaudal CThe morphology and physiology of the oogenesis have been well studied in the vector of Chagas disease Rhodnius prolixus. However, the molecular interactions that regulate the process of egg formation, key for the reproductive cycle of the vector, is still largely unknown. In order to understand the molecular and cellular basis of the oogenesis, we examined the function of the gene Bicaudal C (BicC) during oogenesis and early development of R. prolixus. We show that R. prolixus BicC (Rp-BicC) gene is expressed in the germarium, with cytoplasmic distribution, as well as in the follicular epithelium of the developing oocytes. RNAi silencing of Rp-BicC resulted in sterile females that lay few, small, non-viable eggs. The ovaries are reduced in size and show a disarray of the follicular epithelium. This indicates that Rp-BicC has a central role in the regulation of oogenesis. Although the follicular cells are able to form the chorion, the uptake of vitelline by the oocytes is compromised. We show evidence that the polarity of the follicular epithelium and the endocytic pathway, which are crucial for the proper yolk deposition, are affected. This study provides insights into the molecular mechanisms underlying oocyte development and show that Rp-BicC is important for de developmental of the egg and, therefore, a key player in the reproduction of this insect.Centro de Investigación en Ciencias Veterinarias y AgronómicasFil: Pascual, Agustina. Universidad Nacional del Norte de la Provincia de Buenos Aires. Centro de Bioinvestigaciones; ArgentinaFil: Pascual, Agustina. Comisión de Investigaciones Científicas de la Provincia de Buenos Aires. Centro de Bioinvestigaciones; ArgentinaFil: Vilardo, Emiliano Salvador. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Centro Regional de Estudios Genómicos; ArgentinaFil: Taibo, Catalina Beatriz. Instituto Nacional de Tecnología Agropecuaria (INTA). Centro de Investigación en Ciencias Veterinarias y Agronómicas. Laboratorio Integral de Microscopía; ArgentinaFil: Sabio Y Garcia, Julia Veronica. Instituto Nacional de Tecnología Agropecuaria (INTA). Centro de Investigación en Ciencias Veterinarias y Agronómicas. Laboratorio Integral de Microscopía; ArgentinaFil: Rivera Pomar, Rolando. Universidad Nacional del Norte de la Provincia de Buenos Aires. Centro de Bioinvestigaciones; ArgentinaFil: Rivera Pomar, Rolando. Comisión de Investigaciones Científicas de la Provincia de Buenos Aires. Centro de Bioinvestigaciones; ArgentinaFil: Rivera Pomar, Rolando. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Centro Regional de Estudios Genómicos; ArgentinaSpringer2021-12-13T14:48:46Z2021-12-13T14:48:46Z2021-03info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://hdl.handle.net/20.500.12123/10896https://link.springer.com/article/10.1007%2Fs00427-021-00673-01432-041X0949-944Xhttps://doi.org/10.1007/s00427-021-00673-0Development Genes and Evolution 231 : 33-45. (2021)reponame:INTA Digital (INTA)instname:Instituto Nacional de Tecnología Agropecuariaenginfo:eu-repo/semantics/restrictedAccess2025-09-04T09:49:12Zoai:localhost:20.500.12123/10896instacron:INTAInstitucionalhttp://repositorio.inta.gob.ar/Organismo científico-tecnológicoNo correspondehttp://repositorio.inta.gob.ar/oai/requesttripaldi.nicolas@inta.gob.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:l2025-09-04 09:49:12.934INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuariafalse
dc.title.none.fl_str_mv Bicaudal C is required for the function of the follicular epithelium during oogenesis in Rhodnius prolixus
title Bicaudal C is required for the function of the follicular epithelium during oogenesis in Rhodnius prolixus
spellingShingle Bicaudal C is required for the function of the follicular epithelium during oogenesis in Rhodnius prolixus
Pascual, Agustina
Oogénesis
Rhodnius prolixus
Desarrollo Embrionario
Vitelogénesis
Oogenesis
Embryonic Development
Vitellogenesis
Bicaudal C
title_short Bicaudal C is required for the function of the follicular epithelium during oogenesis in Rhodnius prolixus
title_full Bicaudal C is required for the function of the follicular epithelium during oogenesis in Rhodnius prolixus
title_fullStr Bicaudal C is required for the function of the follicular epithelium during oogenesis in Rhodnius prolixus
title_full_unstemmed Bicaudal C is required for the function of the follicular epithelium during oogenesis in Rhodnius prolixus
title_sort Bicaudal C is required for the function of the follicular epithelium during oogenesis in Rhodnius prolixus
dc.creator.none.fl_str_mv Pascual, Agustina
Vilardo, Emiliano Salvador
Taibo, Catalina Beatriz
Sabio Y Garcia, Julia Veronica
Rivera Pomar, Rolando
author Pascual, Agustina
author_facet Pascual, Agustina
Vilardo, Emiliano Salvador
Taibo, Catalina Beatriz
Sabio Y Garcia, Julia Veronica
Rivera Pomar, Rolando
author_role author
author2 Vilardo, Emiliano Salvador
Taibo, Catalina Beatriz
Sabio Y Garcia, Julia Veronica
Rivera Pomar, Rolando
author2_role author
author
author
author
dc.subject.none.fl_str_mv Oogénesis
Rhodnius prolixus
Desarrollo Embrionario
Vitelogénesis
Oogenesis
Embryonic Development
Vitellogenesis
Bicaudal C
topic Oogénesis
Rhodnius prolixus
Desarrollo Embrionario
Vitelogénesis
Oogenesis
Embryonic Development
Vitellogenesis
Bicaudal C
dc.description.none.fl_txt_mv The morphology and physiology of the oogenesis have been well studied in the vector of Chagas disease Rhodnius prolixus. However, the molecular interactions that regulate the process of egg formation, key for the reproductive cycle of the vector, is still largely unknown. In order to understand the molecular and cellular basis of the oogenesis, we examined the function of the gene Bicaudal C (BicC) during oogenesis and early development of R. prolixus. We show that R. prolixus BicC (Rp-BicC) gene is expressed in the germarium, with cytoplasmic distribution, as well as in the follicular epithelium of the developing oocytes. RNAi silencing of Rp-BicC resulted in sterile females that lay few, small, non-viable eggs. The ovaries are reduced in size and show a disarray of the follicular epithelium. This indicates that Rp-BicC has a central role in the regulation of oogenesis. Although the follicular cells are able to form the chorion, the uptake of vitelline by the oocytes is compromised. We show evidence that the polarity of the follicular epithelium and the endocytic pathway, which are crucial for the proper yolk deposition, are affected. This study provides insights into the molecular mechanisms underlying oocyte development and show that Rp-BicC is important for de developmental of the egg and, therefore, a key player in the reproduction of this insect.
Centro de Investigación en Ciencias Veterinarias y Agronómicas
Fil: Pascual, Agustina. Universidad Nacional del Norte de la Provincia de Buenos Aires. Centro de Bioinvestigaciones; Argentina
Fil: Pascual, Agustina. Comisión de Investigaciones Científicas de la Provincia de Buenos Aires. Centro de Bioinvestigaciones; Argentina
Fil: Vilardo, Emiliano Salvador. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Centro Regional de Estudios Genómicos; Argentina
Fil: Taibo, Catalina Beatriz. Instituto Nacional de Tecnología Agropecuaria (INTA). Centro de Investigación en Ciencias Veterinarias y Agronómicas. Laboratorio Integral de Microscopía; Argentina
Fil: Sabio Y Garcia, Julia Veronica. Instituto Nacional de Tecnología Agropecuaria (INTA). Centro de Investigación en Ciencias Veterinarias y Agronómicas. Laboratorio Integral de Microscopía; Argentina
Fil: Rivera Pomar, Rolando. Universidad Nacional del Norte de la Provincia de Buenos Aires. Centro de Bioinvestigaciones; Argentina
Fil: Rivera Pomar, Rolando. Comisión de Investigaciones Científicas de la Provincia de Buenos Aires. Centro de Bioinvestigaciones; Argentina
Fil: Rivera Pomar, Rolando. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Centro Regional de Estudios Genómicos; Argentina
description The morphology and physiology of the oogenesis have been well studied in the vector of Chagas disease Rhodnius prolixus. However, the molecular interactions that regulate the process of egg formation, key for the reproductive cycle of the vector, is still largely unknown. In order to understand the molecular and cellular basis of the oogenesis, we examined the function of the gene Bicaudal C (BicC) during oogenesis and early development of R. prolixus. We show that R. prolixus BicC (Rp-BicC) gene is expressed in the germarium, with cytoplasmic distribution, as well as in the follicular epithelium of the developing oocytes. RNAi silencing of Rp-BicC resulted in sterile females that lay few, small, non-viable eggs. The ovaries are reduced in size and show a disarray of the follicular epithelium. This indicates that Rp-BicC has a central role in the regulation of oogenesis. Although the follicular cells are able to form the chorion, the uptake of vitelline by the oocytes is compromised. We show evidence that the polarity of the follicular epithelium and the endocytic pathway, which are crucial for the proper yolk deposition, are affected. This study provides insights into the molecular mechanisms underlying oocyte development and show that Rp-BicC is important for de developmental of the egg and, therefore, a key player in the reproduction of this insect.
publishDate 2021
dc.date.none.fl_str_mv 2021-12-13T14:48:46Z
2021-12-13T14:48:46Z
2021-03
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/20.500.12123/10896
https://link.springer.com/article/10.1007%2Fs00427-021-00673-0
1432-041X
0949-944X
https://doi.org/10.1007/s00427-021-00673-0
url http://hdl.handle.net/20.500.12123/10896
https://link.springer.com/article/10.1007%2Fs00427-021-00673-0
https://doi.org/10.1007/s00427-021-00673-0
identifier_str_mv 1432-041X
0949-944X
dc.language.none.fl_str_mv eng
language eng
dc.rights.none.fl_str_mv info:eu-repo/semantics/restrictedAccess
eu_rights_str_mv restrictedAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Springer
publisher.none.fl_str_mv Springer
dc.source.none.fl_str_mv Development Genes and Evolution 231 : 33-45. (2021)
reponame:INTA Digital (INTA)
instname:Instituto Nacional de Tecnología Agropecuaria
reponame_str INTA Digital (INTA)
collection INTA Digital (INTA)
instname_str Instituto Nacional de Tecnología Agropecuaria
repository.name.fl_str_mv INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuaria
repository.mail.fl_str_mv tripaldi.nicolas@inta.gob.ar
_version_ 1842341392925327360
score 12.623145

Publicaciones similares