Pattern of natural introgression in a Nothofagus hybrid zone from South American temperate forests

Autores
El Mujtar, Veronica Andrea; Sola, Georgina Giselle; Aparicio, Alejandro Gabriel; Gallo, Leonardo Ariel
Año de publicación
2017
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Interspecific gene flow is a common phenomenon in Nothofagaceae species; however, the dynamics of introgression in hybrid zones remains largely unknown. We focused on two ecologically and morphologically different Nothofagus species from Patagonia, Nothofagus nervosa and Nothofagus obliqua. In a natural hybrid zone, we established two plots 280mapart in altitude (ca. 1.9 °C difference in mean temperature), and two subplots which captured microsite variation (abundance and spatial distribution of species and predominance of wind direction). We used intensive sampling of individuals (2055, including adults and regeneration) and molecular genotyping of 6 highly species-specific nuclear microsatellites for the identification and classification of hybrids, based on estimates of ancestry and interclass heterozygosity. We evaluated the relative contribution of our sampling effects to variation in hybrid incidence and direction of introgression using generalized linear mixed effects models. We determined that introgressive hybridization occurs at a global rate of 7.8% and that variation was mostly explained by plots (frequency at low altitude was approximately twice that found at high altitude), while it was less influenced by subplots. The high altitude plot was dominated by late-generation backcrosses to N. obliqua (asymmetric bimodality), whereas the low altitude plot consisted of intermediate hybrids (unimodality) and showed asymmetry for introgression between subplots. Differences were not detected between adults and regeneration, suggesting early-acting reproductive isolating barriers. F1 hybrids occur at a global frequency of 3.8%, and are fertile, as the detection of first- and late-generation hybrids indicates.
Estación Experimental Agropecuaria Bariloche
Fil: El Mujtar, Veronica Andrea. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Bariloche. Área Sistemas Forestales. Unidad de Genética Ecológica y Mejoramiento Forestal; Argentina
Fil: Sola, Georgina Giselle. Consejo Nacional de Investigaciones Científicas y Técnicas. CCT-Patagonia Norte; Argentina. Universidad Nacional del Comahue. Cátedra de Ordenación Forestal; Argentina
Fil: Aparicio, Alejandro Gabriel. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Bariloche. Área Sistemas Forestales. Unidad de Genética Ecológica y Mejoramiento Forestal; Argentina
Fil: Gallo, Leonardo Ariel. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Bariloche. Área Sistemas Forestales. Unidad de Genética Ecológica y Mejoramiento Forestal; Argentina
Fuente
Tree Genetics & Genomes 13 : 49 (April 2017)
Materia
Nothofagus
Hibridación
Hybridization
Forests
Bosques
Nothofagus Nervosa
Nothofagus Obliqua
Gradientes Altitudinales
Genética Forestal
Región Patagónica
Nivel de accesibilidad
acceso restringido
Condiciones de uso
Repositorio
INTA Digital (INTA)
Institución
Instituto Nacional de Tecnología Agropecuaria
OAI Identificador
oai:localhost:20.500.12123/5481
Acceder
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oai_identifier_str oai:localhost:20.500.12123/5481
network_acronym_str INTADig
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network_name_str INTA Digital (INTA)
spelling Pattern of natural introgression in a Nothofagus hybrid zone from South American temperate forestsEl Mujtar, Veronica AndreaSola, Georgina GiselleAparicio, Alejandro GabrielGallo, Leonardo ArielNothofagusHibridaciónHybridizationForestsBosquesNothofagus NervosaNothofagus ObliquaGradientes AltitudinalesGenética ForestalRegión PatagónicaInterspecific gene flow is a common phenomenon in Nothofagaceae species; however, the dynamics of introgression in hybrid zones remains largely unknown. We focused on two ecologically and morphologically different Nothofagus species from Patagonia, Nothofagus nervosa and Nothofagus obliqua. In a natural hybrid zone, we established two plots 280mapart in altitude (ca. 1.9 °C difference in mean temperature), and two subplots which captured microsite variation (abundance and spatial distribution of species and predominance of wind direction). We used intensive sampling of individuals (2055, including adults and regeneration) and molecular genotyping of 6 highly species-specific nuclear microsatellites for the identification and classification of hybrids, based on estimates of ancestry and interclass heterozygosity. We evaluated the relative contribution of our sampling effects to variation in hybrid incidence and direction of introgression using generalized linear mixed effects models. We determined that introgressive hybridization occurs at a global rate of 7.8% and that variation was mostly explained by plots (frequency at low altitude was approximately twice that found at high altitude), while it was less influenced by subplots. The high altitude plot was dominated by late-generation backcrosses to N. obliqua (asymmetric bimodality), whereas the low altitude plot consisted of intermediate hybrids (unimodality) and showed asymmetry for introgression between subplots. Differences were not detected between adults and regeneration, suggesting early-acting reproductive isolating barriers. F1 hybrids occur at a global frequency of 3.8%, and are fertile, as the detection of first- and late-generation hybrids indicates.Estación Experimental Agropecuaria BarilocheFil: El Mujtar, Veronica Andrea. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Bariloche. Área Sistemas Forestales. Unidad de Genética Ecológica y Mejoramiento Forestal; ArgentinaFil: Sola, Georgina Giselle. Consejo Nacional de Investigaciones Científicas y Técnicas. CCT-Patagonia Norte; Argentina. Universidad Nacional del Comahue. Cátedra de Ordenación Forestal; ArgentinaFil: Aparicio, Alejandro Gabriel. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Bariloche. Área Sistemas Forestales. Unidad de Genética Ecológica y Mejoramiento Forestal; ArgentinaFil: Gallo, Leonardo Ariel. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Bariloche. Área Sistemas Forestales. Unidad de Genética Ecológica y Mejoramiento Forestal; ArgentinaSpringer-Verlag Berlin Heidelberg2019-07-12T10:52:01Z2019-07-12T10:52:01Z2017-03-29info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://hdl.handle.net/20.500.12123/5481https://link.springer.com/article/10.1007/s11295-017-1132-11614-29421614-2950https://doi.org/10.1007/s11295-017-1132-1Tree Genetics & Genomes 13 : 49 (April 2017)reponame:INTA Digital (INTA)instname:Instituto Nacional de Tecnología Agropecuariaenginfo:eu-repo/semantics/restrictedAccess2025-09-04T09:48:06Zoai:localhost:20.500.12123/5481instacron:INTAInstitucionalhttp://repositorio.inta.gob.ar/Organismo científico-tecnológicoNo correspondehttp://repositorio.inta.gob.ar/oai/requesttripaldi.nicolas@inta.gob.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:l2025-09-04 09:48:07.126INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuariafalse
dc.title.none.fl_str_mv Pattern of natural introgression in a Nothofagus hybrid zone from South American temperate forests
title Pattern of natural introgression in a Nothofagus hybrid zone from South American temperate forests
spellingShingle Pattern of natural introgression in a Nothofagus hybrid zone from South American temperate forests
El Mujtar, Veronica Andrea
Nothofagus
Hibridación
Hybridization
Forests
Bosques
Nothofagus Nervosa
Nothofagus Obliqua
Gradientes Altitudinales
Genética Forestal
Región Patagónica
title_short Pattern of natural introgression in a Nothofagus hybrid zone from South American temperate forests
title_full Pattern of natural introgression in a Nothofagus hybrid zone from South American temperate forests
title_fullStr Pattern of natural introgression in a Nothofagus hybrid zone from South American temperate forests
title_full_unstemmed Pattern of natural introgression in a Nothofagus hybrid zone from South American temperate forests
title_sort Pattern of natural introgression in a Nothofagus hybrid zone from South American temperate forests
dc.creator.none.fl_str_mv El Mujtar, Veronica Andrea
Sola, Georgina Giselle
Aparicio, Alejandro Gabriel
Gallo, Leonardo Ariel
author El Mujtar, Veronica Andrea
author_facet El Mujtar, Veronica Andrea
Sola, Georgina Giselle
Aparicio, Alejandro Gabriel
Gallo, Leonardo Ariel
author_role author
author2 Sola, Georgina Giselle
Aparicio, Alejandro Gabriel
Gallo, Leonardo Ariel
author2_role author
author
author
dc.subject.none.fl_str_mv Nothofagus
Hibridación
Hybridization
Forests
Bosques
Nothofagus Nervosa
Nothofagus Obliqua
Gradientes Altitudinales
Genética Forestal
Región Patagónica
topic Nothofagus
Hibridación
Hybridization
Forests
Bosques
Nothofagus Nervosa
Nothofagus Obliqua
Gradientes Altitudinales
Genética Forestal
Región Patagónica
dc.description.none.fl_txt_mv Interspecific gene flow is a common phenomenon in Nothofagaceae species; however, the dynamics of introgression in hybrid zones remains largely unknown. We focused on two ecologically and morphologically different Nothofagus species from Patagonia, Nothofagus nervosa and Nothofagus obliqua. In a natural hybrid zone, we established two plots 280mapart in altitude (ca. 1.9 °C difference in mean temperature), and two subplots which captured microsite variation (abundance and spatial distribution of species and predominance of wind direction). We used intensive sampling of individuals (2055, including adults and regeneration) and molecular genotyping of 6 highly species-specific nuclear microsatellites for the identification and classification of hybrids, based on estimates of ancestry and interclass heterozygosity. We evaluated the relative contribution of our sampling effects to variation in hybrid incidence and direction of introgression using generalized linear mixed effects models. We determined that introgressive hybridization occurs at a global rate of 7.8% and that variation was mostly explained by plots (frequency at low altitude was approximately twice that found at high altitude), while it was less influenced by subplots. The high altitude plot was dominated by late-generation backcrosses to N. obliqua (asymmetric bimodality), whereas the low altitude plot consisted of intermediate hybrids (unimodality) and showed asymmetry for introgression between subplots. Differences were not detected between adults and regeneration, suggesting early-acting reproductive isolating barriers. F1 hybrids occur at a global frequency of 3.8%, and are fertile, as the detection of first- and late-generation hybrids indicates.
Estación Experimental Agropecuaria Bariloche
Fil: El Mujtar, Veronica Andrea. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Bariloche. Área Sistemas Forestales. Unidad de Genética Ecológica y Mejoramiento Forestal; Argentina
Fil: Sola, Georgina Giselle. Consejo Nacional de Investigaciones Científicas y Técnicas. CCT-Patagonia Norte; Argentina. Universidad Nacional del Comahue. Cátedra de Ordenación Forestal; Argentina
Fil: Aparicio, Alejandro Gabriel. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Bariloche. Área Sistemas Forestales. Unidad de Genética Ecológica y Mejoramiento Forestal; Argentina
Fil: Gallo, Leonardo Ariel. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Bariloche. Área Sistemas Forestales. Unidad de Genética Ecológica y Mejoramiento Forestal; Argentina
description Interspecific gene flow is a common phenomenon in Nothofagaceae species; however, the dynamics of introgression in hybrid zones remains largely unknown. We focused on two ecologically and morphologically different Nothofagus species from Patagonia, Nothofagus nervosa and Nothofagus obliqua. In a natural hybrid zone, we established two plots 280mapart in altitude (ca. 1.9 °C difference in mean temperature), and two subplots which captured microsite variation (abundance and spatial distribution of species and predominance of wind direction). We used intensive sampling of individuals (2055, including adults and regeneration) and molecular genotyping of 6 highly species-specific nuclear microsatellites for the identification and classification of hybrids, based on estimates of ancestry and interclass heterozygosity. We evaluated the relative contribution of our sampling effects to variation in hybrid incidence and direction of introgression using generalized linear mixed effects models. We determined that introgressive hybridization occurs at a global rate of 7.8% and that variation was mostly explained by plots (frequency at low altitude was approximately twice that found at high altitude), while it was less influenced by subplots. The high altitude plot was dominated by late-generation backcrosses to N. obliqua (asymmetric bimodality), whereas the low altitude plot consisted of intermediate hybrids (unimodality) and showed asymmetry for introgression between subplots. Differences were not detected between adults and regeneration, suggesting early-acting reproductive isolating barriers. F1 hybrids occur at a global frequency of 3.8%, and are fertile, as the detection of first- and late-generation hybrids indicates.
publishDate 2017
dc.date.none.fl_str_mv 2017-03-29
2019-07-12T10:52:01Z
2019-07-12T10:52:01Z
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/20.500.12123/5481
https://link.springer.com/article/10.1007/s11295-017-1132-1
1614-2942
1614-2950
https://doi.org/10.1007/s11295-017-1132-1
url http://hdl.handle.net/20.500.12123/5481
https://link.springer.com/article/10.1007/s11295-017-1132-1
https://doi.org/10.1007/s11295-017-1132-1
identifier_str_mv 1614-2942
1614-2950
dc.language.none.fl_str_mv eng
language eng
dc.rights.none.fl_str_mv info:eu-repo/semantics/restrictedAccess
eu_rights_str_mv restrictedAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Springer-Verlag Berlin Heidelberg
publisher.none.fl_str_mv Springer-Verlag Berlin Heidelberg
dc.source.none.fl_str_mv Tree Genetics & Genomes 13 : 49 (April 2017)
reponame:INTA Digital (INTA)
instname:Instituto Nacional de Tecnología Agropecuaria
reponame_str INTA Digital (INTA)
collection INTA Digital (INTA)
instname_str Instituto Nacional de Tecnología Agropecuaria
repository.name.fl_str_mv INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuaria
repository.mail.fl_str_mv tripaldi.nicolas@inta.gob.ar
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score 12.623145

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