Genetic diversity of two native grassland species from Patagonia: implications for domestication and germplasm conservation

Autores
Ignazi, Griselda; Marchelli, Paula; Lopez, Aldana Soledad; Angeli, Juan Pablo; Guidalevich, Verónica; Nagahama, Nicolás; Azpilicueta, Maria Marta
Año de publicación
2026
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The Patagonian steppe grasslands play a central role in livestock production but are increasingly affected by overgrazing and climate change. To contribute to restoration activities, in this study we evaluated the genetic diversity and population structure of two native grasses across central Patagonia using Sequence-Related Amplified Polymorphism (SRAP) markers. The species Bromus setifolius var. pictus and Poa ligularis var. ligularis present different reproductive strategies, being self-pollinating and cross-pollinating, respectively. Nine populations of each species were analyzed with five primer combinations, producing 87 polymorphic bands in B. setifolius and 95 in P. ligularis. At the species level, P. ligularis showed higher genetic diversity values than B. setifolius, which may be associated with their contrasting reproductive systems. A Bayesian analysis did not reveal clear regional clusters but indicated greater genetic homogeneity in P. ligularis. Gene flow may be promoting genetic mixing among populations, whereas the higher differentiation observed in B. setifolius would result from self-pollination reducing variability and enhancing regional structuring. Analysis of molecular variance indicated that most variation was distributed within populations. Significant genetic differences between populations were observed in both species, with higher differentiation in B. setifolius, including a higher number of private bands in western populations, consistent with in situ persistence in glacial refugia. SRAP markers used for the first time in these species were effective in detecting polymorphism and genetic structure and also allowed us to identify the influence of reproductive systems and historical processes, providing a basis for germplasm conservation and breeding programs to restore degraded Patagonian grasslands under increasing aridity.
EEA Bariloche
Fil: Ignazi, Griselda. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Bariloche. Instituto de Investigaciones Forestales y Agropecuarias Bariloche; Argentina
Fil: Ignazi, Griselda. Consejo Nacional de Investigaciones Cientificas y Tecnicas. Instituto de Investigaciones Forestales y Agropecuarias Bariloche; Argentina
Fil: Marchelli, Paula. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Bariloche. Instituto de Investigaciones Forestales y Agropecuarias Bariloche; Argentina
Fil: Marchelli, Paula. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones Forestales y Agropecuarias Bariloche; Argentina
Fil: López, Aldana Soledad. Universidad Nacional de la Patagonia San Juan Bosco. Facultad de Ciencias Naturales y Ciencias de la Salud. Laboratorio de Bioprospección Aplicada en Plantas y Hongos (LaBIAPH); Argentina
Fil: López, Aldana Soledad. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Patagonia Norte; Argentina
Fil: Angeli, Juan Pablo. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agroforestal Esquel; Argentina
Fil: Angeli, Juan Pablo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Patagonia Norte; Argentina
Fil: Guidalevich, Verónica. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Bariloche. Instituto de Investigaciones Forestales y Agropecuarias Bariloche; Argentina
Fil: Guidalevich, Veronica. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones Forestales y Agropecuarias Bariloche; Argentina
Fil: Nagahama, Nicolás. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agroforestal Esquel; Argentina
Fil: Nagahama, Nicolás. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Patagonia Norte; Argentina
Fil: Azpilicueta, Maria Marta. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Bariloche. Instituto de Investigaciones Forestales y Agropecuarias Bariloche; Argentina
Fil: Azpilicueta, Maria Marta. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones Forestales y Agropecuarias Bariloche; Argentina
Fuente
Journal of Arid Environments 235 : 105601. (May 2026)
Materia
Praderas
Pastizales
Variación Genética
Conservación del Germoplasma
Domesticación de Plantas
Marcadores Genéticos
Grasslands
Pastures
Genetic Variation
Germplasm Conservation
Plant Domestication
Genetic Markers
Región Patagónica
Nivel de accesibilidad
acceso restringido
Condiciones de uso
http://creativecommons.org/licenses/by-nc-sa/4.0/
Repositorio
INTA Digital (INTA)
Institución
Instituto Nacional de Tecnología Agropecuaria
OAI Identificador
oai:localhost:20.500.12123/25578
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spelling Genetic diversity of two native grassland species from Patagonia: implications for domestication and germplasm conservationIgnazi, GriseldaMarchelli, PaulaLopez, Aldana SoledadAngeli, Juan PabloGuidalevich, VerónicaNagahama, NicolásAzpilicueta, Maria MartaPraderasPastizalesVariación GenéticaConservación del GermoplasmaDomesticación de PlantasMarcadores GenéticosGrasslandsPasturesGenetic VariationGermplasm ConservationPlant DomesticationGenetic MarkersRegión PatagónicaThe Patagonian steppe grasslands play a central role in livestock production but are increasingly affected by overgrazing and climate change. To contribute to restoration activities, in this study we evaluated the genetic diversity and population structure of two native grasses across central Patagonia using Sequence-Related Amplified Polymorphism (SRAP) markers. The species Bromus setifolius var. pictus and Poa ligularis var. ligularis present different reproductive strategies, being self-pollinating and cross-pollinating, respectively. Nine populations of each species were analyzed with five primer combinations, producing 87 polymorphic bands in B. setifolius and 95 in P. ligularis. At the species level, P. ligularis showed higher genetic diversity values than B. setifolius, which may be associated with their contrasting reproductive systems. A Bayesian analysis did not reveal clear regional clusters but indicated greater genetic homogeneity in P. ligularis. Gene flow may be promoting genetic mixing among populations, whereas the higher differentiation observed in B. setifolius would result from self-pollination reducing variability and enhancing regional structuring. Analysis of molecular variance indicated that most variation was distributed within populations. Significant genetic differences between populations were observed in both species, with higher differentiation in B. setifolius, including a higher number of private bands in western populations, consistent with in situ persistence in glacial refugia. SRAP markers used for the first time in these species were effective in detecting polymorphism and genetic structure and also allowed us to identify the influence of reproductive systems and historical processes, providing a basis for germplasm conservation and breeding programs to restore degraded Patagonian grasslands under increasing aridity.EEA BarilocheFil: Ignazi, Griselda. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Bariloche. Instituto de Investigaciones Forestales y Agropecuarias Bariloche; ArgentinaFil: Ignazi, Griselda. Consejo Nacional de Investigaciones Cientificas y Tecnicas. Instituto de Investigaciones Forestales y Agropecuarias Bariloche; ArgentinaFil: Marchelli, Paula. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Bariloche. Instituto de Investigaciones Forestales y Agropecuarias Bariloche; ArgentinaFil: Marchelli, Paula. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones Forestales y Agropecuarias Bariloche; ArgentinaFil: López, Aldana Soledad. Universidad Nacional de la Patagonia San Juan Bosco. Facultad de Ciencias Naturales y Ciencias de la Salud. Laboratorio de Bioprospección Aplicada en Plantas y Hongos (LaBIAPH); ArgentinaFil: López, Aldana Soledad. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Patagonia Norte; ArgentinaFil: Angeli, Juan Pablo. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agroforestal Esquel; ArgentinaFil: Angeli, Juan Pablo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Patagonia Norte; ArgentinaFil: Guidalevich, Verónica. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Bariloche. Instituto de Investigaciones Forestales y Agropecuarias Bariloche; ArgentinaFil: Guidalevich, Veronica. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones Forestales y Agropecuarias Bariloche; ArgentinaFil: Nagahama, Nicolás. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agroforestal Esquel; ArgentinaFil: Nagahama, Nicolás. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Patagonia Norte; ArgentinaFil: Azpilicueta, Maria Marta. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Bariloche. Instituto de Investigaciones Forestales y Agropecuarias Bariloche; ArgentinaFil: Azpilicueta, Maria Marta. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones Forestales y Agropecuarias Bariloche; ArgentinaElsevier2026-03-26T11:57:56Z2026-03-26T11:57:56Z2026-05info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://hdl.handle.net/20.500.12123/25578https://www.sciencedirect.com/science/article/abs/pii/S01401963260005340140-19631095-922Xhttps://doi.org/10.1016/j.jaridenv.2026.105601Journal of Arid Environments 235 : 105601. (May 2026)reponame:INTA Digital (INTA)instname:Instituto Nacional de Tecnología Agropecuariaenginfo:eu-repograntAgreement/INTA/2019-PE-E6-I142-001, Mejoramiento genético de leguminosas y gramíneas forrajeras para incrementar la productividad y la sustentabilidad de los sistemas agropecuarios de la Argentinainfo:eu-repograntAgreement/INTA/2023-PD-L01-I100, Genética en forrajeras para ambientes y mercados desafiantesinfo:eu-repograntAgreement/INTA/2023-PD-L01-I087, Caracterización de la diversidad genética de plantas, animales y microorganismos mediante herramientas de genómica aplicada.info:eu-repo/semantics/restrictedAccesshttp://creativecommons.org/licenses/by-nc-sa/4.0/Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)2026-04-23T10:40:32Zoai:localhost:20.500.12123/25578instacron:INTAInstitucionalhttp://repositorio.inta.gob.ar/Organismo científico-tecnológicoNo correspondehttp://repositorio.inta.gob.ar/oai/requesttripaldi.nicolas@inta.gob.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:l2026-04-23 10:40:33.589INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuariafalse
dc.title.none.fl_str_mv Genetic diversity of two native grassland species from Patagonia: implications for domestication and germplasm conservation
title Genetic diversity of two native grassland species from Patagonia: implications for domestication and germplasm conservation
spellingShingle Genetic diversity of two native grassland species from Patagonia: implications for domestication and germplasm conservation
Ignazi, Griselda
Praderas
Pastizales
Variación Genética
Conservación del Germoplasma
Domesticación de Plantas
Marcadores Genéticos
Grasslands
Pastures
Genetic Variation
Germplasm Conservation
Plant Domestication
Genetic Markers
Región Patagónica
title_short Genetic diversity of two native grassland species from Patagonia: implications for domestication and germplasm conservation
title_full Genetic diversity of two native grassland species from Patagonia: implications for domestication and germplasm conservation
title_fullStr Genetic diversity of two native grassland species from Patagonia: implications for domestication and germplasm conservation
title_full_unstemmed Genetic diversity of two native grassland species from Patagonia: implications for domestication and germplasm conservation
title_sort Genetic diversity of two native grassland species from Patagonia: implications for domestication and germplasm conservation
dc.creator.none.fl_str_mv Ignazi, Griselda
Marchelli, Paula
Lopez, Aldana Soledad
Angeli, Juan Pablo
Guidalevich, Verónica
Nagahama, Nicolás
Azpilicueta, Maria Marta
author Ignazi, Griselda
author_facet Ignazi, Griselda
Marchelli, Paula
Lopez, Aldana Soledad
Angeli, Juan Pablo
Guidalevich, Verónica
Nagahama, Nicolás
Azpilicueta, Maria Marta
author_role author
author2 Marchelli, Paula
Lopez, Aldana Soledad
Angeli, Juan Pablo
Guidalevich, Verónica
Nagahama, Nicolás
Azpilicueta, Maria Marta
author2_role author
author
author
author
author
author
dc.subject.none.fl_str_mv Praderas
Pastizales
Variación Genética
Conservación del Germoplasma
Domesticación de Plantas
Marcadores Genéticos
Grasslands
Pastures
Genetic Variation
Germplasm Conservation
Plant Domestication
Genetic Markers
Región Patagónica
topic Praderas
Pastizales
Variación Genética
Conservación del Germoplasma
Domesticación de Plantas
Marcadores Genéticos
Grasslands
Pastures
Genetic Variation
Germplasm Conservation
Plant Domestication
Genetic Markers
Región Patagónica
dc.description.none.fl_txt_mv The Patagonian steppe grasslands play a central role in livestock production but are increasingly affected by overgrazing and climate change. To contribute to restoration activities, in this study we evaluated the genetic diversity and population structure of two native grasses across central Patagonia using Sequence-Related Amplified Polymorphism (SRAP) markers. The species Bromus setifolius var. pictus and Poa ligularis var. ligularis present different reproductive strategies, being self-pollinating and cross-pollinating, respectively. Nine populations of each species were analyzed with five primer combinations, producing 87 polymorphic bands in B. setifolius and 95 in P. ligularis. At the species level, P. ligularis showed higher genetic diversity values than B. setifolius, which may be associated with their contrasting reproductive systems. A Bayesian analysis did not reveal clear regional clusters but indicated greater genetic homogeneity in P. ligularis. Gene flow may be promoting genetic mixing among populations, whereas the higher differentiation observed in B. setifolius would result from self-pollination reducing variability and enhancing regional structuring. Analysis of molecular variance indicated that most variation was distributed within populations. Significant genetic differences between populations were observed in both species, with higher differentiation in B. setifolius, including a higher number of private bands in western populations, consistent with in situ persistence in glacial refugia. SRAP markers used for the first time in these species were effective in detecting polymorphism and genetic structure and also allowed us to identify the influence of reproductive systems and historical processes, providing a basis for germplasm conservation and breeding programs to restore degraded Patagonian grasslands under increasing aridity.
EEA Bariloche
Fil: Ignazi, Griselda. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Bariloche. Instituto de Investigaciones Forestales y Agropecuarias Bariloche; Argentina
Fil: Ignazi, Griselda. Consejo Nacional de Investigaciones Cientificas y Tecnicas. Instituto de Investigaciones Forestales y Agropecuarias Bariloche; Argentina
Fil: Marchelli, Paula. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Bariloche. Instituto de Investigaciones Forestales y Agropecuarias Bariloche; Argentina
Fil: Marchelli, Paula. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones Forestales y Agropecuarias Bariloche; Argentina
Fil: López, Aldana Soledad. Universidad Nacional de la Patagonia San Juan Bosco. Facultad de Ciencias Naturales y Ciencias de la Salud. Laboratorio de Bioprospección Aplicada en Plantas y Hongos (LaBIAPH); Argentina
Fil: López, Aldana Soledad. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Patagonia Norte; Argentina
Fil: Angeli, Juan Pablo. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agroforestal Esquel; Argentina
Fil: Angeli, Juan Pablo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Patagonia Norte; Argentina
Fil: Guidalevich, Verónica. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Bariloche. Instituto de Investigaciones Forestales y Agropecuarias Bariloche; Argentina
Fil: Guidalevich, Veronica. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones Forestales y Agropecuarias Bariloche; Argentina
Fil: Nagahama, Nicolás. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agroforestal Esquel; Argentina
Fil: Nagahama, Nicolás. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Patagonia Norte; Argentina
Fil: Azpilicueta, Maria Marta. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Bariloche. Instituto de Investigaciones Forestales y Agropecuarias Bariloche; Argentina
Fil: Azpilicueta, Maria Marta. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones Forestales y Agropecuarias Bariloche; Argentina
description The Patagonian steppe grasslands play a central role in livestock production but are increasingly affected by overgrazing and climate change. To contribute to restoration activities, in this study we evaluated the genetic diversity and population structure of two native grasses across central Patagonia using Sequence-Related Amplified Polymorphism (SRAP) markers. The species Bromus setifolius var. pictus and Poa ligularis var. ligularis present different reproductive strategies, being self-pollinating and cross-pollinating, respectively. Nine populations of each species were analyzed with five primer combinations, producing 87 polymorphic bands in B. setifolius and 95 in P. ligularis. At the species level, P. ligularis showed higher genetic diversity values than B. setifolius, which may be associated with their contrasting reproductive systems. A Bayesian analysis did not reveal clear regional clusters but indicated greater genetic homogeneity in P. ligularis. Gene flow may be promoting genetic mixing among populations, whereas the higher differentiation observed in B. setifolius would result from self-pollination reducing variability and enhancing regional structuring. Analysis of molecular variance indicated that most variation was distributed within populations. Significant genetic differences between populations were observed in both species, with higher differentiation in B. setifolius, including a higher number of private bands in western populations, consistent with in situ persistence in glacial refugia. SRAP markers used for the first time in these species were effective in detecting polymorphism and genetic structure and also allowed us to identify the influence of reproductive systems and historical processes, providing a basis for germplasm conservation and breeding programs to restore degraded Patagonian grasslands under increasing aridity.
publishDate 2026
dc.date.none.fl_str_mv 2026-03-26T11:57:56Z
2026-03-26T11:57:56Z
2026-05
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/20.500.12123/25578
https://www.sciencedirect.com/science/article/abs/pii/S0140196326000534
0140-1963
1095-922X
https://doi.org/10.1016/j.jaridenv.2026.105601
url http://hdl.handle.net/20.500.12123/25578
https://www.sciencedirect.com/science/article/abs/pii/S0140196326000534
https://doi.org/10.1016/j.jaridenv.2026.105601
identifier_str_mv 0140-1963
1095-922X
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repograntAgreement/INTA/2019-PE-E6-I142-001, Mejoramiento genético de leguminosas y gramíneas forrajeras para incrementar la productividad y la sustentabilidad de los sistemas agropecuarios de la Argentina
info:eu-repograntAgreement/INTA/2023-PD-L01-I100, Genética en forrajeras para ambientes y mercados desafiantes
info:eu-repograntAgreement/INTA/2023-PD-L01-I087, Caracterización de la diversidad genética de plantas, animales y microorganismos mediante herramientas de genómica aplicada.
dc.rights.none.fl_str_mv info:eu-repo/semantics/restrictedAccess
http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
eu_rights_str_mv restrictedAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv Journal of Arid Environments 235 : 105601. (May 2026)
reponame:INTA Digital (INTA)
instname:Instituto Nacional de Tecnología Agropecuaria
reponame_str INTA Digital (INTA)
collection INTA Digital (INTA)
instname_str Instituto Nacional de Tecnología Agropecuaria
repository.name.fl_str_mv INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuaria
repository.mail.fl_str_mv tripaldi.nicolas@inta.gob.ar
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