Genomic comparison of two strains of Mycobacterium avium subsp. paratuberculosis with contrasting pathogenic phenotype

Autores
Colombatti Olivieri, Maria Alejandra; Fresia, Pablo; Graña, Martín; Cuerda, Maria Ximena; Nagel, Ariel Gaston; Alvarado Pinedo, Maria Fiorella; Romano, Maria Isabel; Caimi, Karina Cynthia; Berná, Luisa; Santangelo, María De La Paz
Año de publicación
2023
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
In a previous study, we evaluated the degree of virulence of Mycobacterium avium subsp. paratuberculosis (Map) strains isolated from cattle in Argentina in a murine model. This assay allowed us to differentiate between high-virulent MapARG1347 and low-virulent MapARG1543 strains. To corroborate whether the differences in virulence could be attributed to genetic differences between the strains, we performed Whole Genome Sequencing and compared the genomes and gene content between them and determined the differences related to the reference strain MapK10. We found 233 SNPs/INDELS in one or both strains relative to Map K10. The two strains share most of the variations, but we found 15 mutations present in only one of the strains. Considering NS-SNP/INDELS that produced a severe effect in the coding sequence, we focus the analysis on four predicted proteins, putatively related to virulence. Survival of MapARG1347 strain in bMDM was higher than MapARG1543 and was more resistant to acidic pH and H2O2 stresses than MapK10. The genomic differences between the two strains found in genes MAP1203 (a putative peptidoglycan hydrolase), MAP0403 (a putative serine protease) MAP1003c (a member of the PE-PPE family) and MAP4152 (a putative mycofactocin binding protein) could contribute to explain the contrasting phenotype previously observed in mice models.
Instituto de Biotecnología
Fil: Colombatti Olivieri, Maria Alejandra. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina
Fil: Colombatti Olivieri, Maria Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Fresia, Pablo. Institut Pasteur de Montevideo; Uruguay
Fil: Graña, Martín. Institut Pasteur de Montevideo. Unidad de Bioinformática; Uruguay
Fil: Cuerda, Maria Ximena. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina
Fil: Cuerda, Maria Ximena. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Nagel, Ariel Gaston. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina
Fil: Nagel, Ariel Gaston. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Alvarado Pinedo, María Fiorella. Universidad de La Plata. Facultad de Ciencias Veterinarias. Centro de Diagnóstico e Investigaciones Veterinarias; Argentina
Fil: Romano, Maria Isabel. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina
Fil: Romano, Maria Isabel. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Caimi, Karina Cynthia. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina
Fil: Caimi, Karina Cynthia. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Berná, Luisa. Institut Pasteur de Montevideo. Unidad de Biología Molecular; Uruguay
Fil: Santangelo, María De La Paz. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina
Fil: Santangelo, María De La Paz. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fuente
Tuberculosis 138 : 102299 (January 2023)
Materia
Genomics
Mycobacterium avium subsp. paratuberculosis
Virulence
Oxidative Stress
Pathogenicity
Phenotypes
Genómica
Virulencia
Estrés Oxidativo
Patogenicidad
Fenotipos
Nivel de accesibilidad
acceso restringido
Condiciones de uso
http://creativecommons.org/licenses/by-nc-sa/4.0/
Repositorio
INTA Digital (INTA)
Institución
Instituto Nacional de Tecnología Agropecuaria
OAI Identificador
oai:localhost:20.500.12123/15156
Acceder
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oai_identifier_str oai:localhost:20.500.12123/15156
network_acronym_str INTADig
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network_name_str INTA Digital (INTA)
spelling Genomic comparison of two strains of Mycobacterium avium subsp. paratuberculosis with contrasting pathogenic phenotypeColombatti Olivieri, Maria AlejandraFresia, PabloGraña, MartínCuerda, Maria XimenaNagel, Ariel GastonAlvarado Pinedo, Maria FiorellaRomano, Maria IsabelCaimi, Karina CynthiaBerná, LuisaSantangelo, María De La PazGenomicsMycobacterium avium subsp. paratuberculosisVirulenceOxidative StressPathogenicityPhenotypesGenómicaVirulenciaEstrés OxidativoPatogenicidadFenotiposIn a previous study, we evaluated the degree of virulence of Mycobacterium avium subsp. paratuberculosis (Map) strains isolated from cattle in Argentina in a murine model. This assay allowed us to differentiate between high-virulent MapARG1347 and low-virulent MapARG1543 strains. To corroborate whether the differences in virulence could be attributed to genetic differences between the strains, we performed Whole Genome Sequencing and compared the genomes and gene content between them and determined the differences related to the reference strain MapK10. We found 233 SNPs/INDELS in one or both strains relative to Map K10. The two strains share most of the variations, but we found 15 mutations present in only one of the strains. Considering NS-SNP/INDELS that produced a severe effect in the coding sequence, we focus the analysis on four predicted proteins, putatively related to virulence. Survival of MapARG1347 strain in bMDM was higher than MapARG1543 and was more resistant to acidic pH and H2O2 stresses than MapK10. The genomic differences between the two strains found in genes MAP1203 (a putative peptidoglycan hydrolase), MAP0403 (a putative serine protease) MAP1003c (a member of the PE-PPE family) and MAP4152 (a putative mycofactocin binding protein) could contribute to explain the contrasting phenotype previously observed in mice models.Instituto de BiotecnologíaFil: Colombatti Olivieri, Maria Alejandra. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; ArgentinaFil: Colombatti Olivieri, Maria Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Fresia, Pablo. Institut Pasteur de Montevideo; UruguayFil: Graña, Martín. Institut Pasteur de Montevideo. Unidad de Bioinformática; UruguayFil: Cuerda, Maria Ximena. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; ArgentinaFil: Cuerda, Maria Ximena. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Nagel, Ariel Gaston. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; ArgentinaFil: Nagel, Ariel Gaston. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Alvarado Pinedo, María Fiorella. Universidad de La Plata. Facultad de Ciencias Veterinarias. Centro de Diagnóstico e Investigaciones Veterinarias; ArgentinaFil: Romano, Maria Isabel. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; ArgentinaFil: Romano, Maria Isabel. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Caimi, Karina Cynthia. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; ArgentinaFil: Caimi, Karina Cynthia. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Berná, Luisa. Institut Pasteur de Montevideo. Unidad de Biología Molecular; UruguayFil: Santangelo, María De La Paz. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; ArgentinaFil: Santangelo, María De La Paz. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaElsevier2023-09-08T15:32:40Z2023-09-08T15:32:40Z2023-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://hdl.handle.net/20.500.12123/15156https://www.sciencedirect.com/science/article/pii/S14729792220013661472-9792https://doi.org/10.1016/j.tube.2022.102299Tuberculosis 138 : 102299 (January 2023)reponame:INTA Digital (INTA)instname:Instituto Nacional de Tecnología Agropecuariaenginfo:eu-repograntAgreement/INTA/2019-PD-E5-I105-001, Patógenos animales: su interacción con el hospedador y el medio ambiente. Impacto en productividad, ecosistemas, sanidad animal y salud pública en el marco ?Una Salud?info:eu-repo/semantics/restrictedAccesshttp://creativecommons.org/licenses/by-nc-sa/4.0/Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)2025-09-29T13:46:04Zoai:localhost:20.500.12123/15156instacron:INTAInstitucionalhttp://repositorio.inta.gob.ar/Organismo científico-tecnológicoNo correspondehttp://repositorio.inta.gob.ar/oai/requesttripaldi.nicolas@inta.gob.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:l2025-09-29 13:46:05.07INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuariafalse
dc.title.none.fl_str_mv Genomic comparison of two strains of Mycobacterium avium subsp. paratuberculosis with contrasting pathogenic phenotype
title Genomic comparison of two strains of Mycobacterium avium subsp. paratuberculosis with contrasting pathogenic phenotype
spellingShingle Genomic comparison of two strains of Mycobacterium avium subsp. paratuberculosis with contrasting pathogenic phenotype
Colombatti Olivieri, Maria Alejandra
Genomics
Mycobacterium avium subsp. paratuberculosis
Virulence
Oxidative Stress
Pathogenicity
Phenotypes
Genómica
Virulencia
Estrés Oxidativo
Patogenicidad
Fenotipos
title_short Genomic comparison of two strains of Mycobacterium avium subsp. paratuberculosis with contrasting pathogenic phenotype
title_full Genomic comparison of two strains of Mycobacterium avium subsp. paratuberculosis with contrasting pathogenic phenotype
title_fullStr Genomic comparison of two strains of Mycobacterium avium subsp. paratuberculosis with contrasting pathogenic phenotype
title_full_unstemmed Genomic comparison of two strains of Mycobacterium avium subsp. paratuberculosis with contrasting pathogenic phenotype
title_sort Genomic comparison of two strains of Mycobacterium avium subsp. paratuberculosis with contrasting pathogenic phenotype
dc.creator.none.fl_str_mv Colombatti Olivieri, Maria Alejandra
Fresia, Pablo
Graña, Martín
Cuerda, Maria Ximena
Nagel, Ariel Gaston
Alvarado Pinedo, Maria Fiorella
Romano, Maria Isabel
Caimi, Karina Cynthia
Berná, Luisa
Santangelo, María De La Paz
author Colombatti Olivieri, Maria Alejandra
author_facet Colombatti Olivieri, Maria Alejandra
Fresia, Pablo
Graña, Martín
Cuerda, Maria Ximena
Nagel, Ariel Gaston
Alvarado Pinedo, Maria Fiorella
Romano, Maria Isabel
Caimi, Karina Cynthia
Berná, Luisa
Santangelo, María De La Paz
author_role author
author2 Fresia, Pablo
Graña, Martín
Cuerda, Maria Ximena
Nagel, Ariel Gaston
Alvarado Pinedo, Maria Fiorella
Romano, Maria Isabel
Caimi, Karina Cynthia
Berná, Luisa
Santangelo, María De La Paz
author2_role author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Genomics
Mycobacterium avium subsp. paratuberculosis
Virulence
Oxidative Stress
Pathogenicity
Phenotypes
Genómica
Virulencia
Estrés Oxidativo
Patogenicidad
Fenotipos
topic Genomics
Mycobacterium avium subsp. paratuberculosis
Virulence
Oxidative Stress
Pathogenicity
Phenotypes
Genómica
Virulencia
Estrés Oxidativo
Patogenicidad
Fenotipos
dc.description.none.fl_txt_mv In a previous study, we evaluated the degree of virulence of Mycobacterium avium subsp. paratuberculosis (Map) strains isolated from cattle in Argentina in a murine model. This assay allowed us to differentiate between high-virulent MapARG1347 and low-virulent MapARG1543 strains. To corroborate whether the differences in virulence could be attributed to genetic differences between the strains, we performed Whole Genome Sequencing and compared the genomes and gene content between them and determined the differences related to the reference strain MapK10. We found 233 SNPs/INDELS in one or both strains relative to Map K10. The two strains share most of the variations, but we found 15 mutations present in only one of the strains. Considering NS-SNP/INDELS that produced a severe effect in the coding sequence, we focus the analysis on four predicted proteins, putatively related to virulence. Survival of MapARG1347 strain in bMDM was higher than MapARG1543 and was more resistant to acidic pH and H2O2 stresses than MapK10. The genomic differences between the two strains found in genes MAP1203 (a putative peptidoglycan hydrolase), MAP0403 (a putative serine protease) MAP1003c (a member of the PE-PPE family) and MAP4152 (a putative mycofactocin binding protein) could contribute to explain the contrasting phenotype previously observed in mice models.
Instituto de Biotecnología
Fil: Colombatti Olivieri, Maria Alejandra. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina
Fil: Colombatti Olivieri, Maria Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Fresia, Pablo. Institut Pasteur de Montevideo; Uruguay
Fil: Graña, Martín. Institut Pasteur de Montevideo. Unidad de Bioinformática; Uruguay
Fil: Cuerda, Maria Ximena. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina
Fil: Cuerda, Maria Ximena. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Nagel, Ariel Gaston. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina
Fil: Nagel, Ariel Gaston. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Alvarado Pinedo, María Fiorella. Universidad de La Plata. Facultad de Ciencias Veterinarias. Centro de Diagnóstico e Investigaciones Veterinarias; Argentina
Fil: Romano, Maria Isabel. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina
Fil: Romano, Maria Isabel. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Caimi, Karina Cynthia. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina
Fil: Caimi, Karina Cynthia. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Berná, Luisa. Institut Pasteur de Montevideo. Unidad de Biología Molecular; Uruguay
Fil: Santangelo, María De La Paz. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina
Fil: Santangelo, María De La Paz. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
description In a previous study, we evaluated the degree of virulence of Mycobacterium avium subsp. paratuberculosis (Map) strains isolated from cattle in Argentina in a murine model. This assay allowed us to differentiate between high-virulent MapARG1347 and low-virulent MapARG1543 strains. To corroborate whether the differences in virulence could be attributed to genetic differences between the strains, we performed Whole Genome Sequencing and compared the genomes and gene content between them and determined the differences related to the reference strain MapK10. We found 233 SNPs/INDELS in one or both strains relative to Map K10. The two strains share most of the variations, but we found 15 mutations present in only one of the strains. Considering NS-SNP/INDELS that produced a severe effect in the coding sequence, we focus the analysis on four predicted proteins, putatively related to virulence. Survival of MapARG1347 strain in bMDM was higher than MapARG1543 and was more resistant to acidic pH and H2O2 stresses than MapK10. The genomic differences between the two strains found in genes MAP1203 (a putative peptidoglycan hydrolase), MAP0403 (a putative serine protease) MAP1003c (a member of the PE-PPE family) and MAP4152 (a putative mycofactocin binding protein) could contribute to explain the contrasting phenotype previously observed in mice models.
publishDate 2023
dc.date.none.fl_str_mv 2023-09-08T15:32:40Z
2023-09-08T15:32:40Z
2023-01
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/20.500.12123/15156
https://www.sciencedirect.com/science/article/pii/S1472979222001366
1472-9792
https://doi.org/10.1016/j.tube.2022.102299
url http://hdl.handle.net/20.500.12123/15156
https://www.sciencedirect.com/science/article/pii/S1472979222001366
https://doi.org/10.1016/j.tube.2022.102299
identifier_str_mv 1472-9792
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repograntAgreement/INTA/2019-PD-E5-I105-001, Patógenos animales: su interacción con el hospedador y el medio ambiente. Impacto en productividad, ecosistemas, sanidad animal y salud pública en el marco ?Una Salud?
dc.rights.none.fl_str_mv info:eu-repo/semantics/restrictedAccess
http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
eu_rights_str_mv restrictedAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv Tuberculosis 138 : 102299 (January 2023)
reponame:INTA Digital (INTA)
instname:Instituto Nacional de Tecnología Agropecuaria
reponame_str INTA Digital (INTA)
collection INTA Digital (INTA)
instname_str Instituto Nacional de Tecnología Agropecuaria
repository.name.fl_str_mv INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuaria
repository.mail.fl_str_mv tripaldi.nicolas@inta.gob.ar
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