Congenital Tuberculosis in a Stillborn Calf

Autores
Alvarado Pinedo, Maria Fiorella; Di Paolo, Adrián; Zumarraga, Martin Jose; Illanes, Federico; Moyano, Roberto Damian; Peralta, Luis María; Travería, Gabriel
Año de publicación
2026
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Bovine tuberculosis is a zoonotic infectious disease of cattle caused by the bacterium Mycobacterium bovis. In adult cattle, transmission is mainly via the respiratory route, whereas, in young calves, oral infection is also common. Congenital tuberculosis is rare. The purpose of this study was to describe in utero infection of a bovine stillbirth. The fetus was necropsied and tissue samples were processed for histopathology, bacteriology and PCR; a sample of the isolated strain was genotyped using spoligotyping. The whole herd was tuberculin skin tested and the dam’s serum was also assessed for the presence of antibodies against bovine tuberculosis with indirect ELISA. The macroscopic findings in different organs were consistent with tuberculosis. The histopathology demonstrated typical granulomatous lesions in the liver, spleen, hepatic and mediastinal lymph nodes. Acid-fast bacilli were observed in the smears stained using the Ziehl–Neelsen method. The colonies isolated were PCR-positive for IS6110 and the spoligotype corresponded to SB0140. The dam of the stillborn was positive for indirect ELISA and reacted to a single caudal skin test with bovine tuberculin. The fetal infection in utero with Mycobacterium bovis was confirmed through necropsy, histopathology and bacteriology, reinforcing the importance of testing young animals.
Instituto de Biotecnología
Fil: Alvarado Pinedo, María Fiorella. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Centro de Diagnóstico e Investigaciones Veterinarias; Argentina
Fil: Di Paolo, Adrián. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Centro de Diagnóstico e Investigaciones Veterinarias; Argentina
Fil: Zumarraga, Martin Jose. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina
Fil: Zumarraga, Martin Jose. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Agrobiotecnología y Biología Molecular; Argentina
Fil: Illanes, Federico. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Centro de Diagnóstico e Investigaciones Veterinarias; Argentina
Fil: Moyano, Roberto Damian. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina
Fil: Moyano, Roberto Damian. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Agrobiotecnología y Biología Molecular; Argentina
Fil: Peralta, Luis María. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Centro de Diagnóstico e Investigaciones Veterinarias; Argentina
Fil: Travería, Gabriel. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Centro de Diagnóstico e Investigaciones Veterinarias; Argentina
Fuente
Pathogens 15 (1) : 114. (January 2026)
Materia
Tuberculosis Bovina
Enfermedades de los Animales
Ganado Bovino
Ternero
Bovine Tuberculosis
Mycobacterium bovis
Animal Diseases
Cattle
Calves
Foetus
Feto
Nivel de accesibilidad
acceso abierto
Condiciones de uso
http://creativecommons.org/licenses/by-nc-sa/4.0/
Repositorio
INTA Digital (INTA)
Institución
Instituto Nacional de Tecnología Agropecuaria
OAI Identificador
oai:localhost:20.500.12123/25220
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oai_identifier_str oai:localhost:20.500.12123/25220
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network_name_str INTA Digital (INTA)
spelling Congenital Tuberculosis in a Stillborn CalfAlvarado Pinedo, Maria FiorellaDi Paolo, AdriánZumarraga, Martin JoseIllanes, FedericoMoyano, Roberto DamianPeralta, Luis MaríaTravería, GabrielTuberculosis BovinaEnfermedades de los AnimalesGanado BovinoTerneroBovine TuberculosisMycobacterium bovisAnimal DiseasesCattleCalvesFoetusFetoBovine tuberculosis is a zoonotic infectious disease of cattle caused by the bacterium Mycobacterium bovis. In adult cattle, transmission is mainly via the respiratory route, whereas, in young calves, oral infection is also common. Congenital tuberculosis is rare. The purpose of this study was to describe in utero infection of a bovine stillbirth. The fetus was necropsied and tissue samples were processed for histopathology, bacteriology and PCR; a sample of the isolated strain was genotyped using spoligotyping. The whole herd was tuberculin skin tested and the dam’s serum was also assessed for the presence of antibodies against bovine tuberculosis with indirect ELISA. The macroscopic findings in different organs were consistent with tuberculosis. The histopathology demonstrated typical granulomatous lesions in the liver, spleen, hepatic and mediastinal lymph nodes. Acid-fast bacilli were observed in the smears stained using the Ziehl–Neelsen method. The colonies isolated were PCR-positive for IS6110 and the spoligotype corresponded to SB0140. The dam of the stillborn was positive for indirect ELISA and reacted to a single caudal skin test with bovine tuberculin. The fetal infection in utero with Mycobacterium bovis was confirmed through necropsy, histopathology and bacteriology, reinforcing the importance of testing young animals.Instituto de BiotecnologíaFil: Alvarado Pinedo, María Fiorella. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Centro de Diagnóstico e Investigaciones Veterinarias; ArgentinaFil: Di Paolo, Adrián. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Centro de Diagnóstico e Investigaciones Veterinarias; ArgentinaFil: Zumarraga, Martin Jose. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; ArgentinaFil: Zumarraga, Martin Jose. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Agrobiotecnología y Biología Molecular; ArgentinaFil: Illanes, Federico. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Centro de Diagnóstico e Investigaciones Veterinarias; ArgentinaFil: Moyano, Roberto Damian. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; ArgentinaFil: Moyano, Roberto Damian. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Agrobiotecnología y Biología Molecular; ArgentinaFil: Peralta, Luis María. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Centro de Diagnóstico e Investigaciones Veterinarias; ArgentinaFil: Travería, Gabriel. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Centro de Diagnóstico e Investigaciones Veterinarias; ArgentinaMDPI2026-02-18T13:19:51Z2026-02-18T13:19:51Z2026-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://hdl.handle.net/20.500.12123/25220https://www.mdpi.com/2076-0817/15/1/1142076-0817https://doi.org/10.3390/pathogens15010114Pathogens 15 (1) : 114. (January 2026)reponame:INTA Digital (INTA)instname:Instituto Nacional de Tecnología Agropecuariaenginfo:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by-nc-sa/4.0/Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)2026-02-26T11:47:42Zoai:localhost:20.500.12123/25220instacron:INTAInstitucionalhttp://repositorio.inta.gob.ar/Organismo científico-tecnológicoNo correspondehttp://repositorio.inta.gob.ar/oai/requesttripaldi.nicolas@inta.gob.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:l2026-02-26 11:47:42.698INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuariafalse
dc.title.none.fl_str_mv Congenital Tuberculosis in a Stillborn Calf
title Congenital Tuberculosis in a Stillborn Calf
spellingShingle Congenital Tuberculosis in a Stillborn Calf
Alvarado Pinedo, Maria Fiorella
Tuberculosis Bovina
Enfermedades de los Animales
Ganado Bovino
Ternero
Bovine Tuberculosis
Mycobacterium bovis
Animal Diseases
Cattle
Calves
Foetus
Feto
title_short Congenital Tuberculosis in a Stillborn Calf
title_full Congenital Tuberculosis in a Stillborn Calf
title_fullStr Congenital Tuberculosis in a Stillborn Calf
title_full_unstemmed Congenital Tuberculosis in a Stillborn Calf
title_sort Congenital Tuberculosis in a Stillborn Calf
dc.creator.none.fl_str_mv Alvarado Pinedo, Maria Fiorella
Di Paolo, Adrián
Zumarraga, Martin Jose
Illanes, Federico
Moyano, Roberto Damian
Peralta, Luis María
Travería, Gabriel
author Alvarado Pinedo, Maria Fiorella
author_facet Alvarado Pinedo, Maria Fiorella
Di Paolo, Adrián
Zumarraga, Martin Jose
Illanes, Federico
Moyano, Roberto Damian
Peralta, Luis María
Travería, Gabriel
author_role author
author2 Di Paolo, Adrián
Zumarraga, Martin Jose
Illanes, Federico
Moyano, Roberto Damian
Peralta, Luis María
Travería, Gabriel
author2_role author
author
author
author
author
author
dc.subject.none.fl_str_mv Tuberculosis Bovina
Enfermedades de los Animales
Ganado Bovino
Ternero
Bovine Tuberculosis
Mycobacterium bovis
Animal Diseases
Cattle
Calves
Foetus
Feto
topic Tuberculosis Bovina
Enfermedades de los Animales
Ganado Bovino
Ternero
Bovine Tuberculosis
Mycobacterium bovis
Animal Diseases
Cattle
Calves
Foetus
Feto
dc.description.none.fl_txt_mv Bovine tuberculosis is a zoonotic infectious disease of cattle caused by the bacterium Mycobacterium bovis. In adult cattle, transmission is mainly via the respiratory route, whereas, in young calves, oral infection is also common. Congenital tuberculosis is rare. The purpose of this study was to describe in utero infection of a bovine stillbirth. The fetus was necropsied and tissue samples were processed for histopathology, bacteriology and PCR; a sample of the isolated strain was genotyped using spoligotyping. The whole herd was tuberculin skin tested and the dam’s serum was also assessed for the presence of antibodies against bovine tuberculosis with indirect ELISA. The macroscopic findings in different organs were consistent with tuberculosis. The histopathology demonstrated typical granulomatous lesions in the liver, spleen, hepatic and mediastinal lymph nodes. Acid-fast bacilli were observed in the smears stained using the Ziehl–Neelsen method. The colonies isolated were PCR-positive for IS6110 and the spoligotype corresponded to SB0140. The dam of the stillborn was positive for indirect ELISA and reacted to a single caudal skin test with bovine tuberculin. The fetal infection in utero with Mycobacterium bovis was confirmed through necropsy, histopathology and bacteriology, reinforcing the importance of testing young animals.
Instituto de Biotecnología
Fil: Alvarado Pinedo, María Fiorella. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Centro de Diagnóstico e Investigaciones Veterinarias; Argentina
Fil: Di Paolo, Adrián. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Centro de Diagnóstico e Investigaciones Veterinarias; Argentina
Fil: Zumarraga, Martin Jose. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina
Fil: Zumarraga, Martin Jose. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Agrobiotecnología y Biología Molecular; Argentina
Fil: Illanes, Federico. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Centro de Diagnóstico e Investigaciones Veterinarias; Argentina
Fil: Moyano, Roberto Damian. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina
Fil: Moyano, Roberto Damian. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Agrobiotecnología y Biología Molecular; Argentina
Fil: Peralta, Luis María. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Centro de Diagnóstico e Investigaciones Veterinarias; Argentina
Fil: Travería, Gabriel. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Centro de Diagnóstico e Investigaciones Veterinarias; Argentina
description Bovine tuberculosis is a zoonotic infectious disease of cattle caused by the bacterium Mycobacterium bovis. In adult cattle, transmission is mainly via the respiratory route, whereas, in young calves, oral infection is also common. Congenital tuberculosis is rare. The purpose of this study was to describe in utero infection of a bovine stillbirth. The fetus was necropsied and tissue samples were processed for histopathology, bacteriology and PCR; a sample of the isolated strain was genotyped using spoligotyping. The whole herd was tuberculin skin tested and the dam’s serum was also assessed for the presence of antibodies against bovine tuberculosis with indirect ELISA. The macroscopic findings in different organs were consistent with tuberculosis. The histopathology demonstrated typical granulomatous lesions in the liver, spleen, hepatic and mediastinal lymph nodes. Acid-fast bacilli were observed in the smears stained using the Ziehl–Neelsen method. The colonies isolated were PCR-positive for IS6110 and the spoligotype corresponded to SB0140. The dam of the stillborn was positive for indirect ELISA and reacted to a single caudal skin test with bovine tuberculin. The fetal infection in utero with Mycobacterium bovis was confirmed through necropsy, histopathology and bacteriology, reinforcing the importance of testing young animals.
publishDate 2026
dc.date.none.fl_str_mv 2026-02-18T13:19:51Z
2026-02-18T13:19:51Z
2026-01
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/20.500.12123/25220
https://www.mdpi.com/2076-0817/15/1/114
2076-0817
https://doi.org/10.3390/pathogens15010114
url http://hdl.handle.net/20.500.12123/25220
https://www.mdpi.com/2076-0817/15/1/114
https://doi.org/10.3390/pathogens15010114
identifier_str_mv 2076-0817
dc.language.none.fl_str_mv eng
language eng
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
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Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
eu_rights_str_mv openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv MDPI
publisher.none.fl_str_mv MDPI
dc.source.none.fl_str_mv Pathogens 15 (1) : 114. (January 2026)
reponame:INTA Digital (INTA)
instname:Instituto Nacional de Tecnología Agropecuaria
reponame_str INTA Digital (INTA)
collection INTA Digital (INTA)
instname_str Instituto Nacional de Tecnología Agropecuaria
repository.name.fl_str_mv INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuaria
repository.mail.fl_str_mv tripaldi.nicolas@inta.gob.ar
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