Specificity Evaluation of a Polyprotein-Based ELISA Designed for the Detection of Paratuberculosis in Multiple Species

Autores
Moyano, Roberto Damian; Colombatti Olivieri, Maria Alejandra; Bascones González, Mercedes; Garrido, Joseba; Juste, Ramón A.; Alonso, Maria Natalia
Año de publicación
2026
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Paratuberculosis (PTB) is a disease affecting ruminant animals. The etiological agent, Mycobacterium avium subspecies PTB (Map), is a short, Gram-positive, acid-fast bacillus. Due to the nature of PTB, diagnosis often occurs at advanced stages of the disease, following the onset of clinical symptoms and prior dissemination of the agent. The specificity of current humoral diagnostic techniques, such as ELISA, is limited, mainly due to the presence of various species of mycobacteria in the environment and other closely related pathogenic mycobacteria that can interfere with the accurate diagnosis of PTB due to the high rate of gene conservation within the genus. In the present study, we evaluated the effectiveness and specificity of an ELISA based on a Map polyprotein for diagnosing bovine PTB. The efficacy of the polyprotein-based ELISA was assessed using serum samples from healthy, Map-infected, and Mycobacterium bovis (MB)-infected cattle. Specificity was further evaluated using serum samples from healthy and MB-infected wild animals. Our findings revealed that in most cases, the degree of cross-reactivity was negligible or low, particularly in cattle, swine, and goats, while it ranged between 10% and 12.5% in sheep and cervids, respectively. These results suggest that the Map polyprotein used in the ELISA represents a valuable tool for the specific diagnosis of PTB in herds.
Instituto de Biotecnología
Fil: Moyano, Roberto Damian. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina
Fil: Moyano, Roberto Damian. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Colombatti Olivieri, Maria Alejandra. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina
Fil: Colombatti Olivieri, Maria Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Bascones González, Mercedes. Basque Institute for Agricultural Research and Development (NEIKER); España
Fil: Garrido, Joseba. Basque Institute for Agricultural Research and Development (NEIKER); España
Fil: Juste, Ramón A. Basque Institute for Agricultural Research and Development (NEIKER); España
Fil: Alonso Maria Natalia. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular (IABIMO); Argentina
Fil: Alonso Maria Natalia. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fuente
Veterinary Medicine International : 1-7 (First published: 20 January 2026)
Materia
Enfermedades de los Animales
Tuberculosis Bovina
Ganado Bovino
Diagnóstico
Rumiante
Animal Diseases
Bovine Tuberculosis
Paratuberculosis
Cattle
Diagnosis
ELISA
Mycobacterium bovis
Ruminants
Nivel de accesibilidad
acceso abierto
Condiciones de uso
http://creativecommons.org/licenses/by-nc-sa/4.0/
Repositorio
INTA Digital (INTA)
Institución
Instituto Nacional de Tecnología Agropecuaria
OAI Identificador
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spelling Specificity Evaluation of a Polyprotein-Based ELISA Designed for the Detection of Paratuberculosis in Multiple SpeciesMoyano, Roberto DamianColombatti Olivieri, Maria AlejandraBascones González, MercedesGarrido, JosebaJuste, Ramón A.Alonso, Maria NataliaEnfermedades de los AnimalesTuberculosis BovinaGanado BovinoDiagnósticoRumianteAnimal DiseasesBovine TuberculosisParatuberculosisCattleDiagnosisELISAMycobacterium bovisRuminantsParatuberculosis (PTB) is a disease affecting ruminant animals. The etiological agent, Mycobacterium avium subspecies PTB (Map), is a short, Gram-positive, acid-fast bacillus. Due to the nature of PTB, diagnosis often occurs at advanced stages of the disease, following the onset of clinical symptoms and prior dissemination of the agent. The specificity of current humoral diagnostic techniques, such as ELISA, is limited, mainly due to the presence of various species of mycobacteria in the environment and other closely related pathogenic mycobacteria that can interfere with the accurate diagnosis of PTB due to the high rate of gene conservation within the genus. In the present study, we evaluated the effectiveness and specificity of an ELISA based on a Map polyprotein for diagnosing bovine PTB. The efficacy of the polyprotein-based ELISA was assessed using serum samples from healthy, Map-infected, and Mycobacterium bovis (MB)-infected cattle. Specificity was further evaluated using serum samples from healthy and MB-infected wild animals. Our findings revealed that in most cases, the degree of cross-reactivity was negligible or low, particularly in cattle, swine, and goats, while it ranged between 10% and 12.5% in sheep and cervids, respectively. These results suggest that the Map polyprotein used in the ELISA represents a valuable tool for the specific diagnosis of PTB in herds.Instituto de BiotecnologíaFil: Moyano, Roberto Damian. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; ArgentinaFil: Moyano, Roberto Damian. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Colombatti Olivieri, Maria Alejandra. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; ArgentinaFil: Colombatti Olivieri, Maria Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Bascones González, Mercedes. Basque Institute for Agricultural Research and Development (NEIKER); EspañaFil: Garrido, Joseba. Basque Institute for Agricultural Research and Development (NEIKER); EspañaFil: Juste, Ramón A. Basque Institute for Agricultural Research and Development (NEIKER); EspañaFil: Alonso Maria Natalia. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular (IABIMO); ArgentinaFil: Alonso Maria Natalia. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaWiley2026-02-18T12:39:09Z2026-02-18T12:39:09Z2026-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://hdl.handle.net/20.500.12123/25219https://onlinelibrary.wiley.com/doi/10.1155/vmi/73257582090-81132042-0048https://doi.org/10.1155/vmi/7325758Veterinary Medicine International : 1-7 (First published: 20 January 2026)reponame:INTA Digital (INTA)instname:Instituto Nacional de Tecnología Agropecuariaenginfo:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by-nc-sa/4.0/Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)2026-02-26T11:47:42Zoai:localhost:20.500.12123/25219instacron:INTAInstitucionalhttp://repositorio.inta.gob.ar/Organismo científico-tecnológicoNo correspondehttp://repositorio.inta.gob.ar/oai/requesttripaldi.nicolas@inta.gob.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:l2026-02-26 11:47:42.633INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuariafalse
dc.title.none.fl_str_mv Specificity Evaluation of a Polyprotein-Based ELISA Designed for the Detection of Paratuberculosis in Multiple Species
title Specificity Evaluation of a Polyprotein-Based ELISA Designed for the Detection of Paratuberculosis in Multiple Species
spellingShingle Specificity Evaluation of a Polyprotein-Based ELISA Designed for the Detection of Paratuberculosis in Multiple Species
Moyano, Roberto Damian
Enfermedades de los Animales
Tuberculosis Bovina
Ganado Bovino
Diagnóstico
Rumiante
Animal Diseases
Bovine Tuberculosis
Paratuberculosis
Cattle
Diagnosis
ELISA
Mycobacterium bovis
Ruminants
title_short Specificity Evaluation of a Polyprotein-Based ELISA Designed for the Detection of Paratuberculosis in Multiple Species
title_full Specificity Evaluation of a Polyprotein-Based ELISA Designed for the Detection of Paratuberculosis in Multiple Species
title_fullStr Specificity Evaluation of a Polyprotein-Based ELISA Designed for the Detection of Paratuberculosis in Multiple Species
title_full_unstemmed Specificity Evaluation of a Polyprotein-Based ELISA Designed for the Detection of Paratuberculosis in Multiple Species
title_sort Specificity Evaluation of a Polyprotein-Based ELISA Designed for the Detection of Paratuberculosis in Multiple Species
dc.creator.none.fl_str_mv Moyano, Roberto Damian
Colombatti Olivieri, Maria Alejandra
Bascones González, Mercedes
Garrido, Joseba
Juste, Ramón A.
Alonso, Maria Natalia
author Moyano, Roberto Damian
author_facet Moyano, Roberto Damian
Colombatti Olivieri, Maria Alejandra
Bascones González, Mercedes
Garrido, Joseba
Juste, Ramón A.
Alonso, Maria Natalia
author_role author
author2 Colombatti Olivieri, Maria Alejandra
Bascones González, Mercedes
Garrido, Joseba
Juste, Ramón A.
Alonso, Maria Natalia
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv Enfermedades de los Animales
Tuberculosis Bovina
Ganado Bovino
Diagnóstico
Rumiante
Animal Diseases
Bovine Tuberculosis
Paratuberculosis
Cattle
Diagnosis
ELISA
Mycobacterium bovis
Ruminants
topic Enfermedades de los Animales
Tuberculosis Bovina
Ganado Bovino
Diagnóstico
Rumiante
Animal Diseases
Bovine Tuberculosis
Paratuberculosis
Cattle
Diagnosis
ELISA
Mycobacterium bovis
Ruminants
dc.description.none.fl_txt_mv Paratuberculosis (PTB) is a disease affecting ruminant animals. The etiological agent, Mycobacterium avium subspecies PTB (Map), is a short, Gram-positive, acid-fast bacillus. Due to the nature of PTB, diagnosis often occurs at advanced stages of the disease, following the onset of clinical symptoms and prior dissemination of the agent. The specificity of current humoral diagnostic techniques, such as ELISA, is limited, mainly due to the presence of various species of mycobacteria in the environment and other closely related pathogenic mycobacteria that can interfere with the accurate diagnosis of PTB due to the high rate of gene conservation within the genus. In the present study, we evaluated the effectiveness and specificity of an ELISA based on a Map polyprotein for diagnosing bovine PTB. The efficacy of the polyprotein-based ELISA was assessed using serum samples from healthy, Map-infected, and Mycobacterium bovis (MB)-infected cattle. Specificity was further evaluated using serum samples from healthy and MB-infected wild animals. Our findings revealed that in most cases, the degree of cross-reactivity was negligible or low, particularly in cattle, swine, and goats, while it ranged between 10% and 12.5% in sheep and cervids, respectively. These results suggest that the Map polyprotein used in the ELISA represents a valuable tool for the specific diagnosis of PTB in herds.
Instituto de Biotecnología
Fil: Moyano, Roberto Damian. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina
Fil: Moyano, Roberto Damian. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Colombatti Olivieri, Maria Alejandra. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina
Fil: Colombatti Olivieri, Maria Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Bascones González, Mercedes. Basque Institute for Agricultural Research and Development (NEIKER); España
Fil: Garrido, Joseba. Basque Institute for Agricultural Research and Development (NEIKER); España
Fil: Juste, Ramón A. Basque Institute for Agricultural Research and Development (NEIKER); España
Fil: Alonso Maria Natalia. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular (IABIMO); Argentina
Fil: Alonso Maria Natalia. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
description Paratuberculosis (PTB) is a disease affecting ruminant animals. The etiological agent, Mycobacterium avium subspecies PTB (Map), is a short, Gram-positive, acid-fast bacillus. Due to the nature of PTB, diagnosis often occurs at advanced stages of the disease, following the onset of clinical symptoms and prior dissemination of the agent. The specificity of current humoral diagnostic techniques, such as ELISA, is limited, mainly due to the presence of various species of mycobacteria in the environment and other closely related pathogenic mycobacteria that can interfere with the accurate diagnosis of PTB due to the high rate of gene conservation within the genus. In the present study, we evaluated the effectiveness and specificity of an ELISA based on a Map polyprotein for diagnosing bovine PTB. The efficacy of the polyprotein-based ELISA was assessed using serum samples from healthy, Map-infected, and Mycobacterium bovis (MB)-infected cattle. Specificity was further evaluated using serum samples from healthy and MB-infected wild animals. Our findings revealed that in most cases, the degree of cross-reactivity was negligible or low, particularly in cattle, swine, and goats, while it ranged between 10% and 12.5% in sheep and cervids, respectively. These results suggest that the Map polyprotein used in the ELISA represents a valuable tool for the specific diagnosis of PTB in herds.
publishDate 2026
dc.date.none.fl_str_mv 2026-02-18T12:39:09Z
2026-02-18T12:39:09Z
2026-01
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/20.500.12123/25219
https://onlinelibrary.wiley.com/doi/10.1155/vmi/7325758
2090-8113
2042-0048
https://doi.org/10.1155/vmi/7325758
url http://hdl.handle.net/20.500.12123/25219
https://onlinelibrary.wiley.com/doi/10.1155/vmi/7325758
https://doi.org/10.1155/vmi/7325758
identifier_str_mv 2090-8113
2042-0048
dc.language.none.fl_str_mv eng
language eng
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
eu_rights_str_mv openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Wiley
publisher.none.fl_str_mv Wiley
dc.source.none.fl_str_mv Veterinary Medicine International : 1-7 (First published: 20 January 2026)
reponame:INTA Digital (INTA)
instname:Instituto Nacional de Tecnología Agropecuaria
reponame_str INTA Digital (INTA)
collection INTA Digital (INTA)
instname_str Instituto Nacional de Tecnología Agropecuaria
repository.name.fl_str_mv INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuaria
repository.mail.fl_str_mv tripaldi.nicolas@inta.gob.ar
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