Molecular detection of Thecaphora frezzi in peanut (Arachis Hypogaea L.) seeds

Autores
Cazon, Luis Ignacio; Conforto, Erica Cinthia; Fernandez, Franco Daniel; Paredes, Juan Andrés; Rago, Alejandro Mario
Año de publicación
2016
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Thecaphora frezii is the causal agent of peanut smut, important disease in the Argentine production area. The pathogen can be dispersed by kernels, through surface contamination or small lesions. The aim of this work was to design specific primers to develop a Polymerase Chain Reaction assay for the detection of Thecaphora frezii in peanuts kernels. The ITS region of four T. frezii isolates was amplified and sequenced. A consensus sequence was obtained and a pair of primers specific for the pathogen, named TF-2F and TF- 2R, was synthesized. The primers were used for specificity and sensitivity tests. The former test involved a PCR using DNA from T. frezii and other seed-transmitted pathogens (S. rolfsi, S. minor, Fusarium solani and S. sclerotiorum). A 190 bp fragment was obtained for the T. frezii isolates, whereas no amplification was observed for the remaining pathogens. For the sensitivity test, positive results were observed at concentrations up to 3x10-4 pg of the target DNA. The specificity of this method, along with its sensitivity, makes it an important tool for epidemiological studies of peanut smut.
Inst. Patología Vegetal
Fil: Cazon, Luis Ignacio. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina
Fil: Conforto, Erica Cinthia. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina
Fil: Fernandez, Franco Daniel. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina
Fil: Paredes, Juan Andrés. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina
Fil: Rago, Alejandro Mario Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina. Universidad Nacional de Río Cuarto. Facultad de Agronomía y Veterinaria; Argentina
Fuente
Journal of Plant Pathology 98 (2) : 327-330 (2016)
Materia
Enfermedades de las Plantas
Arachis Hypogaea
Genética
Plant Diseases
Genetics
Maní
Thecaphora Frezzi
Nivel de accesibilidad
acceso abierto
Condiciones de uso
http://creativecommons.org/licenses/by-nc-sa/4.0/
Repositorio
INTA Digital (INTA)
Institución
Instituto Nacional de Tecnología Agropecuaria
OAI Identificador
oai:localhost:20.500.12123/1242
Acceder
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oai_identifier_str oai:localhost:20.500.12123/1242
network_acronym_str INTADig
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network_name_str INTA Digital (INTA)
spelling Molecular detection of Thecaphora frezzi in peanut (Arachis Hypogaea L.) seedsCazon, Luis IgnacioConforto, Erica CinthiaFernandez, Franco DanielParedes, Juan AndrésRago, Alejandro MarioEnfermedades de las PlantasArachis HypogaeaGenéticaPlant DiseasesGeneticsManíThecaphora FrezziThecaphora frezii is the causal agent of peanut smut, important disease in the Argentine production area. The pathogen can be dispersed by kernels, through surface contamination or small lesions. The aim of this work was to design specific primers to develop a Polymerase Chain Reaction assay for the detection of Thecaphora frezii in peanuts kernels. The ITS region of four T. frezii isolates was amplified and sequenced. A consensus sequence was obtained and a pair of primers specific for the pathogen, named TF-2F and TF- 2R, was synthesized. The primers were used for specificity and sensitivity tests. The former test involved a PCR using DNA from T. frezii and other seed-transmitted pathogens (S. rolfsi, S. minor, Fusarium solani and S. sclerotiorum). A 190 bp fragment was obtained for the T. frezii isolates, whereas no amplification was observed for the remaining pathogens. For the sensitivity test, positive results were observed at concentrations up to 3x10-4 pg of the target DNA. The specificity of this method, along with its sensitivity, makes it an important tool for epidemiological studies of peanut smut.Inst. Patología VegetalFil: Cazon, Luis Ignacio. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; ArgentinaFil: Conforto, Erica Cinthia. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; ArgentinaFil: Fernandez, Franco Daniel. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; ArgentinaFil: Paredes, Juan Andrés. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; ArgentinaFil: Rago, Alejandro Mario Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina. Universidad Nacional de Río Cuarto. Facultad de Agronomía y Veterinaria; Argentina2017-09-18T17:05:13Z2017-09-18T17:05:13Z2016info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://hdl.handle.net/20.500.12123/1242http://sipav.org/main/jpp/index.php/jpp/article/view/3570/22371125-4653http://dx.doi.org/10.4454/JPP.V98I2.034Journal of Plant Pathology 98 (2) : 327-330 (2016)reponame:INTA Digital (INTA)instname:Instituto Nacional de Tecnología Agropecuariaenginfo:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by-nc-sa/4.0/Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)2025-11-27T08:37:36Zoai:localhost:20.500.12123/1242instacron:INTAInstitucionalhttp://repositorio.inta.gob.ar/Organismo científico-tecnológicoNo correspondehttp://repositorio.inta.gob.ar/oai/requesttripaldi.nicolas@inta.gob.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:l2025-11-27 08:37:37.014INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuariafalse
dc.title.none.fl_str_mv Molecular detection of Thecaphora frezzi in peanut (Arachis Hypogaea L.) seeds
title Molecular detection of Thecaphora frezzi in peanut (Arachis Hypogaea L.) seeds
spellingShingle Molecular detection of Thecaphora frezzi in peanut (Arachis Hypogaea L.) seeds
Cazon, Luis Ignacio
Enfermedades de las Plantas
Arachis Hypogaea
Genética
Plant Diseases
Genetics
Maní
Thecaphora Frezzi
title_short Molecular detection of Thecaphora frezzi in peanut (Arachis Hypogaea L.) seeds
title_full Molecular detection of Thecaphora frezzi in peanut (Arachis Hypogaea L.) seeds
title_fullStr Molecular detection of Thecaphora frezzi in peanut (Arachis Hypogaea L.) seeds
title_full_unstemmed Molecular detection of Thecaphora frezzi in peanut (Arachis Hypogaea L.) seeds
title_sort Molecular detection of Thecaphora frezzi in peanut (Arachis Hypogaea L.) seeds
dc.creator.none.fl_str_mv Cazon, Luis Ignacio
Conforto, Erica Cinthia
Fernandez, Franco Daniel
Paredes, Juan Andrés
Rago, Alejandro Mario
author Cazon, Luis Ignacio
author_facet Cazon, Luis Ignacio
Conforto, Erica Cinthia
Fernandez, Franco Daniel
Paredes, Juan Andrés
Rago, Alejandro Mario
author_role author
author2 Conforto, Erica Cinthia
Fernandez, Franco Daniel
Paredes, Juan Andrés
Rago, Alejandro Mario
author2_role author
author
author
author
dc.subject.none.fl_str_mv Enfermedades de las Plantas
Arachis Hypogaea
Genética
Plant Diseases
Genetics
Maní
Thecaphora Frezzi
topic Enfermedades de las Plantas
Arachis Hypogaea
Genética
Plant Diseases
Genetics
Maní
Thecaphora Frezzi
dc.description.none.fl_txt_mv Thecaphora frezii is the causal agent of peanut smut, important disease in the Argentine production area. The pathogen can be dispersed by kernels, through surface contamination or small lesions. The aim of this work was to design specific primers to develop a Polymerase Chain Reaction assay for the detection of Thecaphora frezii in peanuts kernels. The ITS region of four T. frezii isolates was amplified and sequenced. A consensus sequence was obtained and a pair of primers specific for the pathogen, named TF-2F and TF- 2R, was synthesized. The primers were used for specificity and sensitivity tests. The former test involved a PCR using DNA from T. frezii and other seed-transmitted pathogens (S. rolfsi, S. minor, Fusarium solani and S. sclerotiorum). A 190 bp fragment was obtained for the T. frezii isolates, whereas no amplification was observed for the remaining pathogens. For the sensitivity test, positive results were observed at concentrations up to 3x10-4 pg of the target DNA. The specificity of this method, along with its sensitivity, makes it an important tool for epidemiological studies of peanut smut.
Inst. Patología Vegetal
Fil: Cazon, Luis Ignacio. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina
Fil: Conforto, Erica Cinthia. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina
Fil: Fernandez, Franco Daniel. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina
Fil: Paredes, Juan Andrés. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina
Fil: Rago, Alejandro Mario Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina. Universidad Nacional de Río Cuarto. Facultad de Agronomía y Veterinaria; Argentina
description Thecaphora frezii is the causal agent of peanut smut, important disease in the Argentine production area. The pathogen can be dispersed by kernels, through surface contamination or small lesions. The aim of this work was to design specific primers to develop a Polymerase Chain Reaction assay for the detection of Thecaphora frezii in peanuts kernels. The ITS region of four T. frezii isolates was amplified and sequenced. A consensus sequence was obtained and a pair of primers specific for the pathogen, named TF-2F and TF- 2R, was synthesized. The primers were used for specificity and sensitivity tests. The former test involved a PCR using DNA from T. frezii and other seed-transmitted pathogens (S. rolfsi, S. minor, Fusarium solani and S. sclerotiorum). A 190 bp fragment was obtained for the T. frezii isolates, whereas no amplification was observed for the remaining pathogens. For the sensitivity test, positive results were observed at concentrations up to 3x10-4 pg of the target DNA. The specificity of this method, along with its sensitivity, makes it an important tool for epidemiological studies of peanut smut.
publishDate 2016
dc.date.none.fl_str_mv 2016
2017-09-18T17:05:13Z
2017-09-18T17:05:13Z
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/20.500.12123/1242
http://sipav.org/main/jpp/index.php/jpp/article/view/3570/2237
1125-4653
http://dx.doi.org/10.4454/JPP.V98I2.034
url http://hdl.handle.net/20.500.12123/1242
http://sipav.org/main/jpp/index.php/jpp/article/view/3570/2237
http://dx.doi.org/10.4454/JPP.V98I2.034
identifier_str_mv 1125-4653
dc.language.none.fl_str_mv eng
language eng
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
eu_rights_str_mv openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv Journal of Plant Pathology 98 (2) : 327-330 (2016)
reponame:INTA Digital (INTA)
instname:Instituto Nacional de Tecnología Agropecuaria
reponame_str INTA Digital (INTA)
collection INTA Digital (INTA)
instname_str Instituto Nacional de Tecnología Agropecuaria
repository.name.fl_str_mv INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuaria
repository.mail.fl_str_mv tripaldi.nicolas@inta.gob.ar
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score 13.011256

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