Molecular evidence of Babesia species in Procyon cancrivorus (Carnivora, Procyonidae) in Uruguay

Autores
Thompson, Carolina Soledad; Mangold, Atilio Jose; Félix, María L.; Carvalho, Luis A.; Armúa-Fernández, María Teresita; Venzal, José Manuel
Año de publicación
2018
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The crab-eating raccoon (Procyon cancrivorus) is a carnivore widely distributed from southern Central America to all South American countries except Chile. In the Southern cone of America, P. cancrivorus has been found parasitized by several Amblyomma spp. Particularly, in Uruguay, A. aureolatum is the only tick found in this wild carnivore. Piroplasmid hemoparasites were found in Procyon lotor from North America and Japan. In this work, molecular evidence Babesia sp. DNA was found in blood and tissues from road-killed P. cancrivorus from different locations in Uruguay. PCRs targeting 18S rRNA gene were carried out. Subsequently, the obtained amplicons were sequenced and full-length sequences was assembled. A phylogenetic tree was constructed and revealed that the Babesia sp. found in this work clustered with other 18sRNA sequences of Babesia spp. obtained from P. lotor from Japan and USA, along with Babesia spp. of maned wolf and I. ovatus. This is the first report of molecular evidence of Babesia sp. parasitizing P. cancrivorus.
EEA Rafaela
Fil: Thompson, Carolina Soledad. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rafaela; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Mangold, Atilio Jose. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rafaela; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Félix, María L. Universidad de la República. CENUR Litoral Norte-Salto. Facultad de Veterinaria. Laboratorio de Vectores y Enfermedades Transmitidas; Uruguay
Fil: Carvalho, Luis A. Universidad de la República. CENUR Litoral Norte-Salto. Facultad de Veterinaria. Laboratorio de Vectores y Enfermedades Transmitidas; Uruguay
Fil: Armúa-Fernández, María Teresita. Universidad de la República. CENUR Litoral Norte-Salto. Facultad de Veterinaria. Laboratorio de Vectores y Enfermedades Transmitidas; Uruguay
Fil: Venzal, José Manuel. Universidad de la República. CENUR Litoral Norte-Salto. Facultad de Veterinaria. Laboratorio de Vectores y Enfermedades Transmitidas; Uruguay
Fuente
Veterinary Parasitology: Regional Studies and Reports 13 : 230-233 (August 2018)
Materia
Procyon
Babesia
Amblyomma
Relaciones Huésped Parásito
Genética
Host Parasite Relations
Genetics
Procyon cancrivorus
Mapache
Amblyomma aureolatum
Uruguay
Nivel de accesibilidad
acceso restringido
Condiciones de uso
Repositorio
INTA Digital (INTA)
Institución
Instituto Nacional de Tecnología Agropecuaria
OAI Identificador
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oai_identifier_str oai:localhost:20.500.12123/3014
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spelling Molecular evidence of Babesia species in Procyon cancrivorus (Carnivora, Procyonidae) in UruguayThompson, Carolina SoledadMangold, Atilio JoseFélix, María L.Carvalho, Luis A.Armúa-Fernández, María TeresitaVenzal, José ManuelProcyonBabesiaAmblyommaRelaciones Huésped ParásitoGenéticaHost Parasite RelationsGeneticsProcyon cancrivorusMapacheAmblyomma aureolatumUruguayThe crab-eating raccoon (Procyon cancrivorus) is a carnivore widely distributed from southern Central America to all South American countries except Chile. In the Southern cone of America, P. cancrivorus has been found parasitized by several Amblyomma spp. Particularly, in Uruguay, A. aureolatum is the only tick found in this wild carnivore. Piroplasmid hemoparasites were found in Procyon lotor from North America and Japan. In this work, molecular evidence Babesia sp. DNA was found in blood and tissues from road-killed P. cancrivorus from different locations in Uruguay. PCRs targeting 18S rRNA gene were carried out. Subsequently, the obtained amplicons were sequenced and full-length sequences was assembled. A phylogenetic tree was constructed and revealed that the Babesia sp. found in this work clustered with other 18sRNA sequences of Babesia spp. obtained from P. lotor from Japan and USA, along with Babesia spp. of maned wolf and I. ovatus. This is the first report of molecular evidence of Babesia sp. parasitizing P. cancrivorus.EEA RafaelaFil: Thompson, Carolina Soledad. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rafaela; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Mangold, Atilio Jose. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rafaela; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Félix, María L. Universidad de la República. CENUR Litoral Norte-Salto. Facultad de Veterinaria. Laboratorio de Vectores y Enfermedades Transmitidas; UruguayFil: Carvalho, Luis A. Universidad de la República. CENUR Litoral Norte-Salto. Facultad de Veterinaria. Laboratorio de Vectores y Enfermedades Transmitidas; UruguayFil: Armúa-Fernández, María Teresita. Universidad de la República. CENUR Litoral Norte-Salto. Facultad de Veterinaria. Laboratorio de Vectores y Enfermedades Transmitidas; UruguayFil: Venzal, José Manuel. Universidad de la República. CENUR Litoral Norte-Salto. Facultad de Veterinaria. Laboratorio de Vectores y Enfermedades Transmitidas; Uruguay2018-08-08T13:34:28Z2018-08-08T13:34:28Z2018-08info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttps://www.sciencedirect.com/science/article/pii/S2405939017302770http://hdl.handle.net/20.500.12123/30142405-9390https://doi.org/10.1016/j.vprsr.2018.07.008Veterinary Parasitology: Regional Studies and Reports 13 : 230-233 (August 2018)reponame:INTA Digital (INTA)instname:Instituto Nacional de Tecnología AgropecuariaengUruguay (nation)info:eu-repo/semantics/restrictedAccess2025-09-29T13:44:23Zoai:localhost:20.500.12123/3014instacron:INTAInstitucionalhttp://repositorio.inta.gob.ar/Organismo científico-tecnológicoNo correspondehttp://repositorio.inta.gob.ar/oai/requesttripaldi.nicolas@inta.gob.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:l2025-09-29 13:44:23.877INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuariafalse
dc.title.none.fl_str_mv Molecular evidence of Babesia species in Procyon cancrivorus (Carnivora, Procyonidae) in Uruguay
title Molecular evidence of Babesia species in Procyon cancrivorus (Carnivora, Procyonidae) in Uruguay
spellingShingle Molecular evidence of Babesia species in Procyon cancrivorus (Carnivora, Procyonidae) in Uruguay
Thompson, Carolina Soledad
Procyon
Babesia
Amblyomma
Relaciones Huésped Parásito
Genética
Host Parasite Relations
Genetics
Procyon cancrivorus
Mapache
Amblyomma aureolatum
Uruguay
title_short Molecular evidence of Babesia species in Procyon cancrivorus (Carnivora, Procyonidae) in Uruguay
title_full Molecular evidence of Babesia species in Procyon cancrivorus (Carnivora, Procyonidae) in Uruguay
title_fullStr Molecular evidence of Babesia species in Procyon cancrivorus (Carnivora, Procyonidae) in Uruguay
title_full_unstemmed Molecular evidence of Babesia species in Procyon cancrivorus (Carnivora, Procyonidae) in Uruguay
title_sort Molecular evidence of Babesia species in Procyon cancrivorus (Carnivora, Procyonidae) in Uruguay
dc.creator.none.fl_str_mv Thompson, Carolina Soledad
Mangold, Atilio Jose
Félix, María L.
Carvalho, Luis A.
Armúa-Fernández, María Teresita
Venzal, José Manuel
author Thompson, Carolina Soledad
author_facet Thompson, Carolina Soledad
Mangold, Atilio Jose
Félix, María L.
Carvalho, Luis A.
Armúa-Fernández, María Teresita
Venzal, José Manuel
author_role author
author2 Mangold, Atilio Jose
Félix, María L.
Carvalho, Luis A.
Armúa-Fernández, María Teresita
Venzal, José Manuel
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv Procyon
Babesia
Amblyomma
Relaciones Huésped Parásito
Genética
Host Parasite Relations
Genetics
Procyon cancrivorus
Mapache
Amblyomma aureolatum
Uruguay
topic Procyon
Babesia
Amblyomma
Relaciones Huésped Parásito
Genética
Host Parasite Relations
Genetics
Procyon cancrivorus
Mapache
Amblyomma aureolatum
Uruguay
dc.description.none.fl_txt_mv The crab-eating raccoon (Procyon cancrivorus) is a carnivore widely distributed from southern Central America to all South American countries except Chile. In the Southern cone of America, P. cancrivorus has been found parasitized by several Amblyomma spp. Particularly, in Uruguay, A. aureolatum is the only tick found in this wild carnivore. Piroplasmid hemoparasites were found in Procyon lotor from North America and Japan. In this work, molecular evidence Babesia sp. DNA was found in blood and tissues from road-killed P. cancrivorus from different locations in Uruguay. PCRs targeting 18S rRNA gene were carried out. Subsequently, the obtained amplicons were sequenced and full-length sequences was assembled. A phylogenetic tree was constructed and revealed that the Babesia sp. found in this work clustered with other 18sRNA sequences of Babesia spp. obtained from P. lotor from Japan and USA, along with Babesia spp. of maned wolf and I. ovatus. This is the first report of molecular evidence of Babesia sp. parasitizing P. cancrivorus.
EEA Rafaela
Fil: Thompson, Carolina Soledad. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rafaela; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Mangold, Atilio Jose. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rafaela; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Félix, María L. Universidad de la República. CENUR Litoral Norte-Salto. Facultad de Veterinaria. Laboratorio de Vectores y Enfermedades Transmitidas; Uruguay
Fil: Carvalho, Luis A. Universidad de la República. CENUR Litoral Norte-Salto. Facultad de Veterinaria. Laboratorio de Vectores y Enfermedades Transmitidas; Uruguay
Fil: Armúa-Fernández, María Teresita. Universidad de la República. CENUR Litoral Norte-Salto. Facultad de Veterinaria. Laboratorio de Vectores y Enfermedades Transmitidas; Uruguay
Fil: Venzal, José Manuel. Universidad de la República. CENUR Litoral Norte-Salto. Facultad de Veterinaria. Laboratorio de Vectores y Enfermedades Transmitidas; Uruguay
description The crab-eating raccoon (Procyon cancrivorus) is a carnivore widely distributed from southern Central America to all South American countries except Chile. In the Southern cone of America, P. cancrivorus has been found parasitized by several Amblyomma spp. Particularly, in Uruguay, A. aureolatum is the only tick found in this wild carnivore. Piroplasmid hemoparasites were found in Procyon lotor from North America and Japan. In this work, molecular evidence Babesia sp. DNA was found in blood and tissues from road-killed P. cancrivorus from different locations in Uruguay. PCRs targeting 18S rRNA gene were carried out. Subsequently, the obtained amplicons were sequenced and full-length sequences was assembled. A phylogenetic tree was constructed and revealed that the Babesia sp. found in this work clustered with other 18sRNA sequences of Babesia spp. obtained from P. lotor from Japan and USA, along with Babesia spp. of maned wolf and I. ovatus. This is the first report of molecular evidence of Babesia sp. parasitizing P. cancrivorus.
publishDate 2018
dc.date.none.fl_str_mv 2018-08-08T13:34:28Z
2018-08-08T13:34:28Z
2018-08
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv https://www.sciencedirect.com/science/article/pii/S2405939017302770
http://hdl.handle.net/20.500.12123/3014
2405-9390
https://doi.org/10.1016/j.vprsr.2018.07.008
url https://www.sciencedirect.com/science/article/pii/S2405939017302770
http://hdl.handle.net/20.500.12123/3014
https://doi.org/10.1016/j.vprsr.2018.07.008
identifier_str_mv 2405-9390
dc.language.none.fl_str_mv eng
language eng
dc.rights.none.fl_str_mv info:eu-repo/semantics/restrictedAccess
eu_rights_str_mv restrictedAccess
dc.format.none.fl_str_mv application/pdf
dc.coverage.none.fl_str_mv Uruguay (nation)
dc.source.none.fl_str_mv Veterinary Parasitology: Regional Studies and Reports 13 : 230-233 (August 2018)
reponame:INTA Digital (INTA)
instname:Instituto Nacional de Tecnología Agropecuaria
reponame_str INTA Digital (INTA)
collection INTA Digital (INTA)
instname_str Instituto Nacional de Tecnología Agropecuaria
repository.name.fl_str_mv INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuaria
repository.mail.fl_str_mv tripaldi.nicolas@inta.gob.ar
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score 12.559606

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