Molecular evidence of Babesia species in Procyon cancrivorus (Carnivora, Procyonidae) in Uruguay

Autores
Thompson, Carolina Soledad; Mangold, Atilio Jose; Félix, María L.; Carvalho, Luis; Armúa Fernández, María T.; Venzal, José M.
Año de publicación
2018
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The crab-eating raccoon (Procyon cancrivorus) is a carnivore widely distributed from southern Central America to all South American countries except Chile. In the Southern cone of America, P. cancrivorus has been found parasitized by several Amblyomma spp. Particularly, in Uruguay, A. aureolatum is the only tick found in this wild carnivore. Piroplasmid hemoparasites were found in Procyon lotor from North America and Japan. In this work, molecular evidence Babesia sp. DNA was found in blood and tissues from road-killed P. cancrivorus from different locations in Uruguay. PCRs targeting 18S rRNA gene were carried out. Subsequently, the obtained amplicons were sequenced and full-length sequences was assembled. A phylogenetic tree was constructed and revealed that the Babesia sp. found in this work clustered with other 18sRNA sequences of Babesia spp. obtained from P. lotor from Japan and USA, along with Babesia spp. of maned wolf and I. ovatus. This is the first report of molecular evidence of Babesia sp. parasitizing P. cancrivorus.
Fil: Thompson, Carolina Soledad. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe; Argentina. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Santa Fe. Estación Experimental Agropecuaria Rafaela; Argentina
Fil: Mangold, Atilio Jose. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe; Argentina. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Buenos Aires; Argentina
Fil: Félix, María L.. Universidad de la República; Uruguay
Fil: Carvalho, Luis. Universidad de la República; Uruguay
Fil: Armúa Fernández, María T.. Universidad de la República; Uruguay
Fil: Venzal, José M.. Universidad de la República; Uruguay
Materia
AMBLYOMMA AUREOLATUM
BABESIA SP.
CRAB-EATING RACCOON
PROCYON CANCRIVORUS
URUGUAY
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/84856
Acceder
id CONICETDig_33c9b0c683229158dbb8a30f7e60a578
oai_identifier_str oai:ri.conicet.gov.ar:11336/84856
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Molecular evidence of Babesia species in Procyon cancrivorus (Carnivora, Procyonidae) in UruguayThompson, Carolina SoledadMangold, Atilio JoseFélix, María L.Carvalho, LuisArmúa Fernández, María T.Venzal, José M.AMBLYOMMA AUREOLATUMBABESIA SP.CRAB-EATING RACCOONPROCYON CANCRIVORUSURUGUAYhttps://purl.org/becyt/ford/4.3https://purl.org/becyt/ford/4The crab-eating raccoon (Procyon cancrivorus) is a carnivore widely distributed from southern Central America to all South American countries except Chile. In the Southern cone of America, P. cancrivorus has been found parasitized by several Amblyomma spp. Particularly, in Uruguay, A. aureolatum is the only tick found in this wild carnivore. Piroplasmid hemoparasites were found in Procyon lotor from North America and Japan. In this work, molecular evidence Babesia sp. DNA was found in blood and tissues from road-killed P. cancrivorus from different locations in Uruguay. PCRs targeting 18S rRNA gene were carried out. Subsequently, the obtained amplicons were sequenced and full-length sequences was assembled. A phylogenetic tree was constructed and revealed that the Babesia sp. found in this work clustered with other 18sRNA sequences of Babesia spp. obtained from P. lotor from Japan and USA, along with Babesia spp. of maned wolf and I. ovatus. This is the first report of molecular evidence of Babesia sp. parasitizing P. cancrivorus.Fil: Thompson, Carolina Soledad. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe; Argentina. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Santa Fe. Estación Experimental Agropecuaria Rafaela; ArgentinaFil: Mangold, Atilio Jose. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe; Argentina. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Buenos Aires; ArgentinaFil: Félix, María L.. Universidad de la República; UruguayFil: Carvalho, Luis. Universidad de la República; UruguayFil: Armúa Fernández, María T.. Universidad de la República; UruguayFil: Venzal, José M.. Universidad de la República; UruguayElsevier2018-08info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/84856Thompson, Carolina Soledad; Mangold, Atilio Jose; Félix, María L.; Carvalho, Luis; Armúa Fernández, María T.; et al.; Molecular evidence of Babesia species in Procyon cancrivorus (Carnivora, Procyonidae) in Uruguay; Elsevier; Veterinary Parasitology: Regional Studies and Reports; 13; 8-2018; 230-2332405-9390CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1016/j.vprsr.2018.07.008info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S2405939017302770info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:06:06Zoai:ri.conicet.gov.ar:11336/84856instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:06:07.011CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Molecular evidence of Babesia species in Procyon cancrivorus (Carnivora, Procyonidae) in Uruguay
title Molecular evidence of Babesia species in Procyon cancrivorus (Carnivora, Procyonidae) in Uruguay
spellingShingle Molecular evidence of Babesia species in Procyon cancrivorus (Carnivora, Procyonidae) in Uruguay
Thompson, Carolina Soledad
AMBLYOMMA AUREOLATUM
BABESIA SP.
CRAB-EATING RACCOON
PROCYON CANCRIVORUS
URUGUAY
title_short Molecular evidence of Babesia species in Procyon cancrivorus (Carnivora, Procyonidae) in Uruguay
title_full Molecular evidence of Babesia species in Procyon cancrivorus (Carnivora, Procyonidae) in Uruguay
title_fullStr Molecular evidence of Babesia species in Procyon cancrivorus (Carnivora, Procyonidae) in Uruguay
title_full_unstemmed Molecular evidence of Babesia species in Procyon cancrivorus (Carnivora, Procyonidae) in Uruguay
title_sort Molecular evidence of Babesia species in Procyon cancrivorus (Carnivora, Procyonidae) in Uruguay
dc.creator.none.fl_str_mv Thompson, Carolina Soledad
Mangold, Atilio Jose
Félix, María L.
Carvalho, Luis
Armúa Fernández, María T.
Venzal, José M.
author Thompson, Carolina Soledad
author_facet Thompson, Carolina Soledad
Mangold, Atilio Jose
Félix, María L.
Carvalho, Luis
Armúa Fernández, María T.
Venzal, José M.
author_role author
author2 Mangold, Atilio Jose
Félix, María L.
Carvalho, Luis
Armúa Fernández, María T.
Venzal, José M.
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv AMBLYOMMA AUREOLATUM
BABESIA SP.
CRAB-EATING RACCOON
PROCYON CANCRIVORUS
URUGUAY
topic AMBLYOMMA AUREOLATUM
BABESIA SP.
CRAB-EATING RACCOON
PROCYON CANCRIVORUS
URUGUAY
purl_subject.fl_str_mv https://purl.org/becyt/ford/4.3
https://purl.org/becyt/ford/4
dc.description.none.fl_txt_mv The crab-eating raccoon (Procyon cancrivorus) is a carnivore widely distributed from southern Central America to all South American countries except Chile. In the Southern cone of America, P. cancrivorus has been found parasitized by several Amblyomma spp. Particularly, in Uruguay, A. aureolatum is the only tick found in this wild carnivore. Piroplasmid hemoparasites were found in Procyon lotor from North America and Japan. In this work, molecular evidence Babesia sp. DNA was found in blood and tissues from road-killed P. cancrivorus from different locations in Uruguay. PCRs targeting 18S rRNA gene were carried out. Subsequently, the obtained amplicons were sequenced and full-length sequences was assembled. A phylogenetic tree was constructed and revealed that the Babesia sp. found in this work clustered with other 18sRNA sequences of Babesia spp. obtained from P. lotor from Japan and USA, along with Babesia spp. of maned wolf and I. ovatus. This is the first report of molecular evidence of Babesia sp. parasitizing P. cancrivorus.
Fil: Thompson, Carolina Soledad. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe; Argentina. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Santa Fe. Estación Experimental Agropecuaria Rafaela; Argentina
Fil: Mangold, Atilio Jose. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe; Argentina. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Buenos Aires; Argentina
Fil: Félix, María L.. Universidad de la República; Uruguay
Fil: Carvalho, Luis. Universidad de la República; Uruguay
Fil: Armúa Fernández, María T.. Universidad de la República; Uruguay
Fil: Venzal, José M.. Universidad de la República; Uruguay
description The crab-eating raccoon (Procyon cancrivorus) is a carnivore widely distributed from southern Central America to all South American countries except Chile. In the Southern cone of America, P. cancrivorus has been found parasitized by several Amblyomma spp. Particularly, in Uruguay, A. aureolatum is the only tick found in this wild carnivore. Piroplasmid hemoparasites were found in Procyon lotor from North America and Japan. In this work, molecular evidence Babesia sp. DNA was found in blood and tissues from road-killed P. cancrivorus from different locations in Uruguay. PCRs targeting 18S rRNA gene were carried out. Subsequently, the obtained amplicons were sequenced and full-length sequences was assembled. A phylogenetic tree was constructed and revealed that the Babesia sp. found in this work clustered with other 18sRNA sequences of Babesia spp. obtained from P. lotor from Japan and USA, along with Babesia spp. of maned wolf and I. ovatus. This is the first report of molecular evidence of Babesia sp. parasitizing P. cancrivorus.
publishDate 2018
dc.date.none.fl_str_mv 2018-08
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/84856
Thompson, Carolina Soledad; Mangold, Atilio Jose; Félix, María L.; Carvalho, Luis; Armúa Fernández, María T.; et al.; Molecular evidence of Babesia species in Procyon cancrivorus (Carnivora, Procyonidae) in Uruguay; Elsevier; Veterinary Parasitology: Regional Studies and Reports; 13; 8-2018; 230-233
2405-9390
CONICET Digital
CONICET
url http://hdl.handle.net/11336/84856
identifier_str_mv Thompson, Carolina Soledad; Mangold, Atilio Jose; Félix, María L.; Carvalho, Luis; Armúa Fernández, María T.; et al.; Molecular evidence of Babesia species in Procyon cancrivorus (Carnivora, Procyonidae) in Uruguay; Elsevier; Veterinary Parasitology: Regional Studies and Reports; 13; 8-2018; 230-233
2405-9390
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1016/j.vprsr.2018.07.008
info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S2405939017302770
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1844613905905614848
score 13.070432

Publicaciones similares