A recombinant multi-antigen vaccine formulation containing Babesia bovis merozoite surface antigens MSA-2a1, MSA- 2b and MSA-2c elicits invasion-inhibitory antibodies and IFN-γ pro...

Autores
Gimenez, Alba Marina; Françoso, Katia S.; Ersching, Jonatan; Icimoto, Marcelo Y.; Oliveira, Vitor; Rodriguez, Anabel Elisa; Schnittger, Leonhard; Florin-Christensen, Mónica; Rodrigues, Mauricio M.; Soares, Irene S.
Año de publicación
2016
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Background: Babesia bovis is a tick-transmitted protozoan hemoparasite and the causative agent of bovine babesiosis, a potential risk to more than 500 million cattle worldwide. The vaccines currently available are based on attenuated parasites, which are difficult to produce, and are only recommended for use in bovines under one year of age. When used in older animals, these vaccines may cause life-threatening clinical symptoms and eventually death. The development of a multi-subunit recombinant vaccine against B. bovis would be attractive from an economic standpoint and, most importantly, could be recommended for animals of any age. In the present study, recombinant ectodomains of MSA-2a1, MSA-2b and MSA-2c antigens were expressed in Pichia pastoris yeast as secreted soluble peptides. Results: The antigens were purified to homogeneity, and biochemically and immunologically characterized. A vaccine formulation was obtained by emulsifying a mixture of the three peptides with the adjuvant Montanide ISA 720, which elicited high IgG antibody titers against each of the above antigens. IgG antibodies generated against each MSA-antigen recognized merozoites and significantly inhibited the invasion of bovine erythrocytes. Cellular immune responses were also detected, which were characterized by splenic and lymph node CD4+ T cells producing IFN-γ and TNF-α upon stimulation with the antigens MSA-2a1 or MSA-2c.
Inst. de Patobiología
Fil: Gimenez, Alba Marina. Universidade Federal de São Paulo. Escola Paulista de Medicina. Departamento de Microbiologia, Imunologia e Parasitologia; Brasil
Fil: Françoso, Katia S. Universidade de Sao Paulo. Faculdade de Ciências Farmacêuticas. Departamento de Análises Clínicas e Toxicológicas; Brasil
Fil: Ersching, Jonatan. Universidade Federal de São Paulo. Escola Paulista de Medicina. Departamento de Microbiologia, Imunologia e Parasitologia; Brasil
Fil: Icimoto, Marcelo Y. Universidade Federal de Sao Paulo. Departamento de Biofísica; Brasil
Fil: Oliveira, Vitor. Universidade Federal de Sao Paulo. Departamento de Biofísica; Brasil
Fil: Rodriguez, Anabel Elisa. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología; Argentina
Fil: Schnittger, Leonhard. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Florin-Christensen, Mónica. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Françoso, Katia S. Universidade de São Paulo. Faculdade de Ciências Farmacêuticas. Departamento de Análises Clínicas e Toxicológicas; Brasil
Fil: Rodrigues, Mauricio M. Universidade Federal de São Paulo. Escola Paulista de Medicina. Departamento de Microbiologia, Imunologia e Parasitologia; Brasil
Fil: Soares, Irene S. Universidade de Sao Paulo. Faculdade de Ciências Farmacêuticas. Departamento de Análises Clínicas e Toxicológicas; Brasil
Fuente
Parasites and vectors 9 (1) : 577. (2016)
Materia
Babesia Bovis
Enfermedades de los Animales
Vacuna
Antígenos
Anticuerpos
Vaccines
Antigens
Antibodies
Animal Diseases
Nivel de accesibilidad
acceso abierto
Condiciones de uso
http://creativecommons.org/licenses/by-nc-sa/4.0/
Repositorio
INTA Digital (INTA)
Institución
Instituto Nacional de Tecnología Agropecuaria
OAI Identificador
oai:localhost:20.500.12123/1000
Acceder
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oai_identifier_str oai:localhost:20.500.12123/1000
network_acronym_str INTADig
repository_id_str l
network_name_str INTA Digital (INTA)
spelling A recombinant multi-antigen vaccine formulation containing Babesia bovis merozoite surface antigens MSA-2a1, MSA- 2b and MSA-2c elicits invasion-inhibitory antibodies and IFN-γ producing cellsGimenez, Alba MarinaFrançoso, Katia S.Ersching, JonatanIcimoto, Marcelo Y.Oliveira, VitorRodriguez, Anabel ElisaSchnittger, LeonhardFlorin-Christensen, MónicaRodrigues, Mauricio M.Soares, Irene S.Babesia BovisEnfermedades de los AnimalesVacunaAntígenosAnticuerposVaccinesAntigensAntibodiesAnimal DiseasesBackground: Babesia bovis is a tick-transmitted protozoan hemoparasite and the causative agent of bovine babesiosis, a potential risk to more than 500 million cattle worldwide. The vaccines currently available are based on attenuated parasites, which are difficult to produce, and are only recommended for use in bovines under one year of age. When used in older animals, these vaccines may cause life-threatening clinical symptoms and eventually death. The development of a multi-subunit recombinant vaccine against B. bovis would be attractive from an economic standpoint and, most importantly, could be recommended for animals of any age. In the present study, recombinant ectodomains of MSA-2a1, MSA-2b and MSA-2c antigens were expressed in Pichia pastoris yeast as secreted soluble peptides. Results: The antigens were purified to homogeneity, and biochemically and immunologically characterized. A vaccine formulation was obtained by emulsifying a mixture of the three peptides with the adjuvant Montanide ISA 720, which elicited high IgG antibody titers against each of the above antigens. IgG antibodies generated against each MSA-antigen recognized merozoites and significantly inhibited the invasion of bovine erythrocytes. Cellular immune responses were also detected, which were characterized by splenic and lymph node CD4+ T cells producing IFN-γ and TNF-α upon stimulation with the antigens MSA-2a1 or MSA-2c.Inst. de PatobiologíaFil: Gimenez, Alba Marina. Universidade Federal de São Paulo. Escola Paulista de Medicina. Departamento de Microbiologia, Imunologia e Parasitologia; BrasilFil: Françoso, Katia S. Universidade de Sao Paulo. Faculdade de Ciências Farmacêuticas. Departamento de Análises Clínicas e Toxicológicas; BrasilFil: Ersching, Jonatan. Universidade Federal de São Paulo. Escola Paulista de Medicina. Departamento de Microbiologia, Imunologia e Parasitologia; BrasilFil: Icimoto, Marcelo Y. Universidade Federal de Sao Paulo. Departamento de Biofísica; BrasilFil: Oliveira, Vitor. Universidade Federal de Sao Paulo. Departamento de Biofísica; BrasilFil: Rodriguez, Anabel Elisa. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología; ArgentinaFil: Schnittger, Leonhard. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Florin-Christensen, Mónica. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Françoso, Katia S. Universidade de São Paulo. Faculdade de Ciências Farmacêuticas. Departamento de Análises Clínicas e Toxicológicas; BrasilFil: Rodrigues, Mauricio M. Universidade Federal de São Paulo. Escola Paulista de Medicina. Departamento de Microbiologia, Imunologia e Parasitologia; BrasilFil: Soares, Irene S. Universidade de Sao Paulo. Faculdade de Ciências Farmacêuticas. Departamento de Análises Clínicas e Toxicológicas; Brasil2017-08-22T12:48:55Z2017-08-22T12:48:55Z2016info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://hdl.handle.net/20.500.12123/1000https://parasitesandvectors.biomedcentral.com/articles/10.1186/s13071-016-1862-1http://ri.conicet.gov.ar/handle/11336/182731756-3305https://doi.org/10.1186/s13071-016-1862-1Parasites and vectors 9 (1) : 577. (2016)reponame:INTA Digital (INTA)instname:Instituto Nacional de Tecnología Agropecuariaenginfo:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by-nc-sa/4.0/Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)2025-09-29T13:44:09Zoai:localhost:20.500.12123/1000instacron:INTAInstitucionalhttp://repositorio.inta.gob.ar/Organismo científico-tecnológicoNo correspondehttp://repositorio.inta.gob.ar/oai/requesttripaldi.nicolas@inta.gob.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:l2025-09-29 13:44:09.38INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuariafalse
dc.title.none.fl_str_mv A recombinant multi-antigen vaccine formulation containing Babesia bovis merozoite surface antigens MSA-2a1, MSA- 2b and MSA-2c elicits invasion-inhibitory antibodies and IFN-γ producing cells
title A recombinant multi-antigen vaccine formulation containing Babesia bovis merozoite surface antigens MSA-2a1, MSA- 2b and MSA-2c elicits invasion-inhibitory antibodies and IFN-γ producing cells
spellingShingle A recombinant multi-antigen vaccine formulation containing Babesia bovis merozoite surface antigens MSA-2a1, MSA- 2b and MSA-2c elicits invasion-inhibitory antibodies and IFN-γ producing cells
Gimenez, Alba Marina
Babesia Bovis
Enfermedades de los Animales
Vacuna
Antígenos
Anticuerpos
Vaccines
Antigens
Antibodies
Animal Diseases
title_short A recombinant multi-antigen vaccine formulation containing Babesia bovis merozoite surface antigens MSA-2a1, MSA- 2b and MSA-2c elicits invasion-inhibitory antibodies and IFN-γ producing cells
title_full A recombinant multi-antigen vaccine formulation containing Babesia bovis merozoite surface antigens MSA-2a1, MSA- 2b and MSA-2c elicits invasion-inhibitory antibodies and IFN-γ producing cells
title_fullStr A recombinant multi-antigen vaccine formulation containing Babesia bovis merozoite surface antigens MSA-2a1, MSA- 2b and MSA-2c elicits invasion-inhibitory antibodies and IFN-γ producing cells
title_full_unstemmed A recombinant multi-antigen vaccine formulation containing Babesia bovis merozoite surface antigens MSA-2a1, MSA- 2b and MSA-2c elicits invasion-inhibitory antibodies and IFN-γ producing cells
title_sort A recombinant multi-antigen vaccine formulation containing Babesia bovis merozoite surface antigens MSA-2a1, MSA- 2b and MSA-2c elicits invasion-inhibitory antibodies and IFN-γ producing cells
dc.creator.none.fl_str_mv Gimenez, Alba Marina
Françoso, Katia S.
Ersching, Jonatan
Icimoto, Marcelo Y.
Oliveira, Vitor
Rodriguez, Anabel Elisa
Schnittger, Leonhard
Florin-Christensen, Mónica
Rodrigues, Mauricio M.
Soares, Irene S.
author Gimenez, Alba Marina
author_facet Gimenez, Alba Marina
Françoso, Katia S.
Ersching, Jonatan
Icimoto, Marcelo Y.
Oliveira, Vitor
Rodriguez, Anabel Elisa
Schnittger, Leonhard
Florin-Christensen, Mónica
Rodrigues, Mauricio M.
Soares, Irene S.
author_role author
author2 Françoso, Katia S.
Ersching, Jonatan
Icimoto, Marcelo Y.
Oliveira, Vitor
Rodriguez, Anabel Elisa
Schnittger, Leonhard
Florin-Christensen, Mónica
Rodrigues, Mauricio M.
Soares, Irene S.
author2_role author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Babesia Bovis
Enfermedades de los Animales
Vacuna
Antígenos
Anticuerpos
Vaccines
Antigens
Antibodies
Animal Diseases
topic Babesia Bovis
Enfermedades de los Animales
Vacuna
Antígenos
Anticuerpos
Vaccines
Antigens
Antibodies
Animal Diseases
dc.description.none.fl_txt_mv Background: Babesia bovis is a tick-transmitted protozoan hemoparasite and the causative agent of bovine babesiosis, a potential risk to more than 500 million cattle worldwide. The vaccines currently available are based on attenuated parasites, which are difficult to produce, and are only recommended for use in bovines under one year of age. When used in older animals, these vaccines may cause life-threatening clinical symptoms and eventually death. The development of a multi-subunit recombinant vaccine against B. bovis would be attractive from an economic standpoint and, most importantly, could be recommended for animals of any age. In the present study, recombinant ectodomains of MSA-2a1, MSA-2b and MSA-2c antigens were expressed in Pichia pastoris yeast as secreted soluble peptides. Results: The antigens were purified to homogeneity, and biochemically and immunologically characterized. A vaccine formulation was obtained by emulsifying a mixture of the three peptides with the adjuvant Montanide ISA 720, which elicited high IgG antibody titers against each of the above antigens. IgG antibodies generated against each MSA-antigen recognized merozoites and significantly inhibited the invasion of bovine erythrocytes. Cellular immune responses were also detected, which were characterized by splenic and lymph node CD4+ T cells producing IFN-γ and TNF-α upon stimulation with the antigens MSA-2a1 or MSA-2c.
Inst. de Patobiología
Fil: Gimenez, Alba Marina. Universidade Federal de São Paulo. Escola Paulista de Medicina. Departamento de Microbiologia, Imunologia e Parasitologia; Brasil
Fil: Françoso, Katia S. Universidade de Sao Paulo. Faculdade de Ciências Farmacêuticas. Departamento de Análises Clínicas e Toxicológicas; Brasil
Fil: Ersching, Jonatan. Universidade Federal de São Paulo. Escola Paulista de Medicina. Departamento de Microbiologia, Imunologia e Parasitologia; Brasil
Fil: Icimoto, Marcelo Y. Universidade Federal de Sao Paulo. Departamento de Biofísica; Brasil
Fil: Oliveira, Vitor. Universidade Federal de Sao Paulo. Departamento de Biofísica; Brasil
Fil: Rodriguez, Anabel Elisa. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología; Argentina
Fil: Schnittger, Leonhard. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Florin-Christensen, Mónica. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Françoso, Katia S. Universidade de São Paulo. Faculdade de Ciências Farmacêuticas. Departamento de Análises Clínicas e Toxicológicas; Brasil
Fil: Rodrigues, Mauricio M. Universidade Federal de São Paulo. Escola Paulista de Medicina. Departamento de Microbiologia, Imunologia e Parasitologia; Brasil
Fil: Soares, Irene S. Universidade de Sao Paulo. Faculdade de Ciências Farmacêuticas. Departamento de Análises Clínicas e Toxicológicas; Brasil
description Background: Babesia bovis is a tick-transmitted protozoan hemoparasite and the causative agent of bovine babesiosis, a potential risk to more than 500 million cattle worldwide. The vaccines currently available are based on attenuated parasites, which are difficult to produce, and are only recommended for use in bovines under one year of age. When used in older animals, these vaccines may cause life-threatening clinical symptoms and eventually death. The development of a multi-subunit recombinant vaccine against B. bovis would be attractive from an economic standpoint and, most importantly, could be recommended for animals of any age. In the present study, recombinant ectodomains of MSA-2a1, MSA-2b and MSA-2c antigens were expressed in Pichia pastoris yeast as secreted soluble peptides. Results: The antigens were purified to homogeneity, and biochemically and immunologically characterized. A vaccine formulation was obtained by emulsifying a mixture of the three peptides with the adjuvant Montanide ISA 720, which elicited high IgG antibody titers against each of the above antigens. IgG antibodies generated against each MSA-antigen recognized merozoites and significantly inhibited the invasion of bovine erythrocytes. Cellular immune responses were also detected, which were characterized by splenic and lymph node CD4+ T cells producing IFN-γ and TNF-α upon stimulation with the antigens MSA-2a1 or MSA-2c.
publishDate 2016
dc.date.none.fl_str_mv 2016
2017-08-22T12:48:55Z
2017-08-22T12:48:55Z
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/20.500.12123/1000
https://parasitesandvectors.biomedcentral.com/articles/10.1186/s13071-016-1862-1
http://ri.conicet.gov.ar/handle/11336/18273
1756-3305
https://doi.org/10.1186/s13071-016-1862-1
url http://hdl.handle.net/20.500.12123/1000
https://parasitesandvectors.biomedcentral.com/articles/10.1186/s13071-016-1862-1
http://ri.conicet.gov.ar/handle/11336/18273
https://doi.org/10.1186/s13071-016-1862-1
identifier_str_mv 1756-3305
dc.language.none.fl_str_mv eng
language eng
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
eu_rights_str_mv openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv Parasites and vectors 9 (1) : 577. (2016)
reponame:INTA Digital (INTA)
instname:Instituto Nacional de Tecnología Agropecuaria
reponame_str INTA Digital (INTA)
collection INTA Digital (INTA)
instname_str Instituto Nacional de Tecnología Agropecuaria
repository.name.fl_str_mv INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuaria
repository.mail.fl_str_mv tripaldi.nicolas@inta.gob.ar
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