Changes in hematological, biochemical, and blood gases prameters in response to progressive inclusion of nitrate in the diet of Holstein calves

Autores
Ortiz Chura, Abimael; Marcoppido, Gisela Ariana; Gere, José Ignacio; Depetris, Gustavo; Stefañuk Bahamonde, Francisco José; Trangoni, Marcos David; Cravero, Silvio Lorenzo Pedro; Faverin, Claudia; Cataldi, Angel Adrian; Ceron Cucchi, Maria Esperanza
Año de publicación
2021
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Background and Aim: Nitrate (NO3-) reduces enteric methane emissions and could be a source of non-protein nitrogen in ruminant feeds. Nonetheless, it has a potential toxic effect that could compromise animal health and production. The purpose of this study was to determine the effects of progressive inclusion of NO3- in the diet on the hematological, biochemical, and blood gases parameters, in turn, the effects on feed intake and live weight gain (LWG) in Holstein calves. Materials and Methods: Eighteen Holstein heifers and steers (nine animals/treatment) were maintained in individual pens for 45 days. Animals were randomly allocated to either a control or nitrate diet (ND) (containing 15 g of NO3-/kg of dry matter [DM]). The biochemical parameters and blood gases were analyzed only in the NO3- group on days: -1, 1, 7, 13, 19, and 25 corresponding to 0, 20, 40, 60, 80, and 100% of the total inclusion of NO3- in the diet, respectively. In addition, DM intake (DMI) and LWG were evaluated among dietary treatments. Results: Feeding the ND did not influence DMI or LWG (p>0.05). Methemoglobin (MetHb) and deoxyhemoglobin increased according to the NO3- concentrations in the diet (p<0.05), while an opposite effect was observed for oxyhemoglobin and carboxyhemoglobin (p<0.05). Hematocrit levels decreased (p<0.05), while albumin, alanine aminotransferase, and gamma-glutamyl transpeptidase concentrations were not modified (p>0.05). However, glucose, urea, aspartate aminotransferase (AST), and retinol concentrations increased (p<0.05) according to the NO3- concentrations in the diet.
Instituto de Patobiología
Fil: Ortiz Chura, Abimael. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología; Argentina
Fil: Ortiz Chura, Abimael. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Marcoppido, Gisela Ariana. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología; Argentina
Fil: Marcoppido, Gisela Ariana. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Gere, José. Universidad Tecnológica Nacional. División Investigación y Desarrollo de Ingenierías; Argentina
Fil: Gere, José. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Depetris, Gustavo. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina
Fil: Stefañuk Bahamonte, Francisco José. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina
Fil: Trangoni, Marcos David. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular. Argentina
Fil: Trangoni, Marcos David. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Cravero, Silvio Lorenzo Pedro. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina
Fil: Cravero, Silvio Lorenzo Pedro. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Faverin, Claudia. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina
Fil: Cataldi, Angel Adrian. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina
Fil: Cataldi, Angel Adrian. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Ceron Cucchi, Maria Esperanza. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología; Argentina
Fil: Ceron Cucchi, Maria Esperanza. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fuente
Veterinary World 14 (1) : 61-69 (Enero 2021)
Materia
Liver Function Tests
Nitrates
Diet
Calves
Toxicity
Pruebas de Función Hepática
Nitratos
Dieta
Ternero
Toxicidad
Raza Holstein
Nivel de accesibilidad
acceso abierto
Condiciones de uso
http://creativecommons.org/licenses/by-nc-sa/4.0/
Repositorio
INTA Digital (INTA)
Institución
Instituto Nacional de Tecnología Agropecuaria
OAI Identificador
oai:localhost:20.500.12123/9104
Acceder
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oai_identifier_str oai:localhost:20.500.12123/9104
network_acronym_str INTADig
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network_name_str INTA Digital (INTA)
spelling Changes in hematological, biochemical, and blood gases prameters in response to progressive inclusion of nitrate in the diet of Holstein calvesOrtiz Chura, AbimaelMarcoppido, Gisela ArianaGere, José IgnacioDepetris, GustavoStefañuk Bahamonde, Francisco JoséTrangoni, Marcos DavidCravero, Silvio Lorenzo PedroFaverin, ClaudiaCataldi, Angel AdrianCeron Cucchi, Maria EsperanzaLiver Function TestsNitratesDietCalvesToxicityPruebas de Función HepáticaNitratosDietaTerneroToxicidadRaza HolsteinBackground and Aim: Nitrate (NO3-) reduces enteric methane emissions and could be a source of non-protein nitrogen in ruminant feeds. Nonetheless, it has a potential toxic effect that could compromise animal health and production. The purpose of this study was to determine the effects of progressive inclusion of NO3- in the diet on the hematological, biochemical, and blood gases parameters, in turn, the effects on feed intake and live weight gain (LWG) in Holstein calves. Materials and Methods: Eighteen Holstein heifers and steers (nine animals/treatment) were maintained in individual pens for 45 days. Animals were randomly allocated to either a control or nitrate diet (ND) (containing 15 g of NO3-/kg of dry matter [DM]). The biochemical parameters and blood gases were analyzed only in the NO3- group on days: -1, 1, 7, 13, 19, and 25 corresponding to 0, 20, 40, 60, 80, and 100% of the total inclusion of NO3- in the diet, respectively. In addition, DM intake (DMI) and LWG were evaluated among dietary treatments. Results: Feeding the ND did not influence DMI or LWG (p>0.05). Methemoglobin (MetHb) and deoxyhemoglobin increased according to the NO3- concentrations in the diet (p<0.05), while an opposite effect was observed for oxyhemoglobin and carboxyhemoglobin (p<0.05). Hematocrit levels decreased (p<0.05), while albumin, alanine aminotransferase, and gamma-glutamyl transpeptidase concentrations were not modified (p>0.05). However, glucose, urea, aspartate aminotransferase (AST), and retinol concentrations increased (p<0.05) according to the NO3- concentrations in the diet.Instituto de PatobiologíaFil: Ortiz Chura, Abimael. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología; ArgentinaFil: Ortiz Chura, Abimael. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Marcoppido, Gisela Ariana. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología; ArgentinaFil: Marcoppido, Gisela Ariana. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Gere, José. Universidad Tecnológica Nacional. División Investigación y Desarrollo de Ingenierías; ArgentinaFil: Gere, José. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Depetris, Gustavo. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; ArgentinaFil: Stefañuk Bahamonte, Francisco José. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; ArgentinaFil: Trangoni, Marcos David. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular. ArgentinaFil: Trangoni, Marcos David. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Cravero, Silvio Lorenzo Pedro. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; ArgentinaFil: Cravero, Silvio Lorenzo Pedro. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Faverin, Claudia. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; ArgentinaFil: Cataldi, Angel Adrian. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; ArgentinaFil: Cataldi, Angel Adrian. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Ceron Cucchi, Maria Esperanza. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología; ArgentinaFil: Ceron Cucchi, Maria Esperanza. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaVeterinary World Publisher2021-04-16T11:00:15Z2021-04-16T11:00:15Z2021-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://hdl.handle.net/20.500.12123/9104http://www.veterinaryworld.org/Vol.14/January-2021/8.html2231-09160972-8988https://www.doi.org/10.14202/vetworld.2021.61-69Veterinary World 14 (1) : 61-69 (Enero 2021)reponame:INTA Digital (INTA)instname:Instituto Nacional de Tecnología Agropecuariaenginfo:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by-nc-sa/4.0/Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)2025-10-23T11:17:33Zoai:localhost:20.500.12123/9104instacron:INTAInstitucionalhttp://repositorio.inta.gob.ar/Organismo científico-tecnológicoNo correspondehttp://repositorio.inta.gob.ar/oai/requesttripaldi.nicolas@inta.gob.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:l2025-10-23 11:17:34.232INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuariafalse
dc.title.none.fl_str_mv Changes in hematological, biochemical, and blood gases prameters in response to progressive inclusion of nitrate in the diet of Holstein calves
title Changes in hematological, biochemical, and blood gases prameters in response to progressive inclusion of nitrate in the diet of Holstein calves
spellingShingle Changes in hematological, biochemical, and blood gases prameters in response to progressive inclusion of nitrate in the diet of Holstein calves
Ortiz Chura, Abimael
Liver Function Tests
Nitrates
Diet
Calves
Toxicity
Pruebas de Función Hepática
Nitratos
Dieta
Ternero
Toxicidad
Raza Holstein
title_short Changes in hematological, biochemical, and blood gases prameters in response to progressive inclusion of nitrate in the diet of Holstein calves
title_full Changes in hematological, biochemical, and blood gases prameters in response to progressive inclusion of nitrate in the diet of Holstein calves
title_fullStr Changes in hematological, biochemical, and blood gases prameters in response to progressive inclusion of nitrate in the diet of Holstein calves
title_full_unstemmed Changes in hematological, biochemical, and blood gases prameters in response to progressive inclusion of nitrate in the diet of Holstein calves
title_sort Changes in hematological, biochemical, and blood gases prameters in response to progressive inclusion of nitrate in the diet of Holstein calves
dc.creator.none.fl_str_mv Ortiz Chura, Abimael
Marcoppido, Gisela Ariana
Gere, José Ignacio
Depetris, Gustavo
Stefañuk Bahamonde, Francisco José
Trangoni, Marcos David
Cravero, Silvio Lorenzo Pedro
Faverin, Claudia
Cataldi, Angel Adrian
Ceron Cucchi, Maria Esperanza
author Ortiz Chura, Abimael
author_facet Ortiz Chura, Abimael
Marcoppido, Gisela Ariana
Gere, José Ignacio
Depetris, Gustavo
Stefañuk Bahamonde, Francisco José
Trangoni, Marcos David
Cravero, Silvio Lorenzo Pedro
Faverin, Claudia
Cataldi, Angel Adrian
Ceron Cucchi, Maria Esperanza
author_role author
author2 Marcoppido, Gisela Ariana
Gere, José Ignacio
Depetris, Gustavo
Stefañuk Bahamonde, Francisco José
Trangoni, Marcos David
Cravero, Silvio Lorenzo Pedro
Faverin, Claudia
Cataldi, Angel Adrian
Ceron Cucchi, Maria Esperanza
author2_role author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Liver Function Tests
Nitrates
Diet
Calves
Toxicity
Pruebas de Función Hepática
Nitratos
Dieta
Ternero
Toxicidad
Raza Holstein
topic Liver Function Tests
Nitrates
Diet
Calves
Toxicity
Pruebas de Función Hepática
Nitratos
Dieta
Ternero
Toxicidad
Raza Holstein
dc.description.none.fl_txt_mv Background and Aim: Nitrate (NO3-) reduces enteric methane emissions and could be a source of non-protein nitrogen in ruminant feeds. Nonetheless, it has a potential toxic effect that could compromise animal health and production. The purpose of this study was to determine the effects of progressive inclusion of NO3- in the diet on the hematological, biochemical, and blood gases parameters, in turn, the effects on feed intake and live weight gain (LWG) in Holstein calves. Materials and Methods: Eighteen Holstein heifers and steers (nine animals/treatment) were maintained in individual pens for 45 days. Animals were randomly allocated to either a control or nitrate diet (ND) (containing 15 g of NO3-/kg of dry matter [DM]). The biochemical parameters and blood gases were analyzed only in the NO3- group on days: -1, 1, 7, 13, 19, and 25 corresponding to 0, 20, 40, 60, 80, and 100% of the total inclusion of NO3- in the diet, respectively. In addition, DM intake (DMI) and LWG were evaluated among dietary treatments. Results: Feeding the ND did not influence DMI or LWG (p>0.05). Methemoglobin (MetHb) and deoxyhemoglobin increased according to the NO3- concentrations in the diet (p<0.05), while an opposite effect was observed for oxyhemoglobin and carboxyhemoglobin (p<0.05). Hematocrit levels decreased (p<0.05), while albumin, alanine aminotransferase, and gamma-glutamyl transpeptidase concentrations were not modified (p>0.05). However, glucose, urea, aspartate aminotransferase (AST), and retinol concentrations increased (p<0.05) according to the NO3- concentrations in the diet.
Instituto de Patobiología
Fil: Ortiz Chura, Abimael. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología; Argentina
Fil: Ortiz Chura, Abimael. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Marcoppido, Gisela Ariana. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología; Argentina
Fil: Marcoppido, Gisela Ariana. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Gere, José. Universidad Tecnológica Nacional. División Investigación y Desarrollo de Ingenierías; Argentina
Fil: Gere, José. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Depetris, Gustavo. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina
Fil: Stefañuk Bahamonte, Francisco José. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina
Fil: Trangoni, Marcos David. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular. Argentina
Fil: Trangoni, Marcos David. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Cravero, Silvio Lorenzo Pedro. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina
Fil: Cravero, Silvio Lorenzo Pedro. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Faverin, Claudia. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina
Fil: Cataldi, Angel Adrian. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina
Fil: Cataldi, Angel Adrian. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Ceron Cucchi, Maria Esperanza. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología; Argentina
Fil: Ceron Cucchi, Maria Esperanza. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
description Background and Aim: Nitrate (NO3-) reduces enteric methane emissions and could be a source of non-protein nitrogen in ruminant feeds. Nonetheless, it has a potential toxic effect that could compromise animal health and production. The purpose of this study was to determine the effects of progressive inclusion of NO3- in the diet on the hematological, biochemical, and blood gases parameters, in turn, the effects on feed intake and live weight gain (LWG) in Holstein calves. Materials and Methods: Eighteen Holstein heifers and steers (nine animals/treatment) were maintained in individual pens for 45 days. Animals were randomly allocated to either a control or nitrate diet (ND) (containing 15 g of NO3-/kg of dry matter [DM]). The biochemical parameters and blood gases were analyzed only in the NO3- group on days: -1, 1, 7, 13, 19, and 25 corresponding to 0, 20, 40, 60, 80, and 100% of the total inclusion of NO3- in the diet, respectively. In addition, DM intake (DMI) and LWG were evaluated among dietary treatments. Results: Feeding the ND did not influence DMI or LWG (p>0.05). Methemoglobin (MetHb) and deoxyhemoglobin increased according to the NO3- concentrations in the diet (p<0.05), while an opposite effect was observed for oxyhemoglobin and carboxyhemoglobin (p<0.05). Hematocrit levels decreased (p<0.05), while albumin, alanine aminotransferase, and gamma-glutamyl transpeptidase concentrations were not modified (p>0.05). However, glucose, urea, aspartate aminotransferase (AST), and retinol concentrations increased (p<0.05) according to the NO3- concentrations in the diet.
publishDate 2021
dc.date.none.fl_str_mv 2021-04-16T11:00:15Z
2021-04-16T11:00:15Z
2021-01
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/20.500.12123/9104
http://www.veterinaryworld.org/Vol.14/January-2021/8.html
2231-0916
0972-8988
https://www.doi.org/10.14202/vetworld.2021.61-69
url http://hdl.handle.net/20.500.12123/9104
http://www.veterinaryworld.org/Vol.14/January-2021/8.html
https://www.doi.org/10.14202/vetworld.2021.61-69
identifier_str_mv 2231-0916
0972-8988
dc.language.none.fl_str_mv eng
language eng
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
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Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
eu_rights_str_mv openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Veterinary World Publisher
publisher.none.fl_str_mv Veterinary World Publisher
dc.source.none.fl_str_mv Veterinary World 14 (1) : 61-69 (Enero 2021)
reponame:INTA Digital (INTA)
instname:Instituto Nacional de Tecnología Agropecuaria
reponame_str INTA Digital (INTA)
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instname_str Instituto Nacional de Tecnología Agropecuaria
repository.name.fl_str_mv INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuaria
repository.mail.fl_str_mv tripaldi.nicolas@inta.gob.ar
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